New Mediterranean Marine biodiversity records (December, 2013)

M. Bilecenoglu [et al.]Based on recent biodiversity studies carried out in different parts of the Mediterranean, the following 19 species are included as new records on the floral or faunal lists of the relevant ecosystems: the green algae Penicillus capitatus (Maltese waters); the nemertean Amphiporus allucens (Iberian Peninsula, Spain); the salp Salpa maxima (Syria); the opistobranchs Felimida britoi and Berghia coerulescens (Aegean Sea, Greece); the dusky shark Carcharhinus obscurus (central-west Mediterranean and Ionian Sea, Italy); Randall’s threadfin bream Nemipterus randalli, the broadbanded cardinalfish Apogon fasciatus and the goby Gobius kolombatovici (Aegean Sea, Turkey); the reticulated leatherjack Stephanolepis diaspros and the halacarid Agaue chevreuxi (Sea of Marmara, Turkey); the slimy liagora Ganonema farinosum, the yellowstripe barracuda Sphyraena chrysotaenia, the rayed pearl oyster Pinctada imbricata radiata and the Persian conch Conomurex persicus (south-eastern Crete, Greece); the blenny Microlipophrys dalmatinus and the bastard grunt Pomadasys incisus (Ionian Sea, Italy); the brown shrimp Farfantepenaeus aztecus (north-eastern Levant, Turkey); the blue-crab Callinectes sapidus (Corfu, Ionian Sea, Greece). In addition, the findings of the following rare species improve currently available biogeographical knowledge: the oceanic pufferfish Lagocephalus lagocephalus (Malta); the yellow sea chub Kyphosus incisor (Almuñécar coast of Spain); the basking shark Cetorhinus maximus and the shortfin mako Isurus oxyrinchus (north-eastern Levant, Turkey).Peer reviewe

An onto-phylogenetic journey through the life history of flying squids (Cephalopoda: Ommastrephidae)

Versió amb diverses seccions retallades, per drets de l'editorFlying squids develop all its life cycle in the water column, as planktonic paralarvae and then as nektonic subadults and adults. In this Ph. D. Thesis, light was shed over several poorly understood aspects of the ontogeny and phylogeny of the Family Ommastrephidae. The mechanism of sperm migration from spermatangia to the female seminal receptacles was studied. Spermatozoa are able to actively migrate between both structures. The morphology of the hatchling of three Mediterranean ommastrephid species was studied based on embryos obtained by in vitro fertilization and a dichotomous key was develop to identify NE Atlantic species. The first feeding diet of paralarvae was assessed through laser-capture microdissection and DNA metabarcoding. The results indicate an ontogenetic shift from detritivorism to active predation. Molecular data indicate that the taxonomic name Ommastrephes bartramii actually hides four biological species. These advances in scientific knowledge have potential applications for a better understanding of the ecology, physiology, biodiversity and fishery science that will foster a deeper understanding of flying squids.Las potas desarrollan todo su ciclo vital en la columna de agua, como paralarvas planctónicas y luego como subadultos y adultos nectónicos. En esta Tesis Doctoral, se ha arrojado luz sobre algunos aspectos poco estudiados de la ontogenia y la filogenia de Familia Ommastrephidae. Se estudió el mecanismo de migración espermática desde los espermatangios hasta los receptáculos seminales de la hembra. Los espermatozoides son capaces de migrar activamente entre ambas estructuras. Se estudió la morfología de los recién nacidos de tres especies mediterráneas de pota basándose en embriones generados por fecundación in vitro y se desarrolló una clave dicotómica para identificar las especies del Atlántico NE. Se estudió la primera alimentación de las paralarvas mediante una combinación de microdisección láser y DNA metabarcoding: los resultados indican una fase detritívora seguida de un cambio ontogenético hacia la depredación activa. Nuestros datos moleculares muestran que bajo el nombre taxonómico Ommastrephes bartramii en realidad se esconden cuatro especies biológicas. Estos avances en el conocimiento científico tienen aplicaciones potenciales para una mejor comprensión de la ecología, fisiología, biodiversidad y ciencia pesquera de las potasPostprint (published version

Primera cita del elusivo calamar oceánico Thysanoteuthis rhombus Troschel, 1857 (Cephalopoda: Thysanoteuthidae) en la costa catalana

The diamondback squid Thysanoteuthis rhombus Troschel, 1857 is a large and elusive oceanic squid species. Here, we provide the first record of the species for the Catalan coast and integrate this finding in the current knowledge of the species in close areas.El chipirón volantín (Thysanoteuthis rhombus Troschel, 1857) es una especie de calamar oceánico grande y elusivo. En este trabajo se proporciona el primer registro de la especie para la costa catalana y se discute en el contexto de registros de la especie en zonas cercanas

Spatiotemporal pattern of brain electrical activity related to immediate and delayed episodic memory retrieval

In the present study we used the event-related brain potentials (ERP) technique and eLORETA (exact low-resolution electromagnetic tomography) method in order to characterize and compare the performance and the spatiotemporal pattern of the brain electrical activity related to the immediate episodic retrieval of information (words) that is being learned relative to delayed episodic retrieval twenty-minutes later. For this purpose, 16 young participants carried out an old/new word recognition task with source memory (word colour). The task included an immediate memory phase (with three study-test blocks) followed (20 min later) by a delayed memory phase with one test block. The behavioural data showed progressive learning and consolidation of the information (old words) during the immediate memory phase. The ERP data to correctly identified old words for which the colour was subsequently recollected (H/H) compared to the correctly rejected new words (CR) showed: (1) a significant more positive-going potential in the 500–675 ms post-stimulus interval (parietal old/new effect, related to recollection), and (2) a more negative-going potential in the 950–1850 ms interval (LPN effect, related to retrieval and post-retrieval processes). The eLORETA data also revealed that the successful recognition of old words (and probably retrieval of their colour) was accompanied by activation of (1) left medial temporal (parahippocampal gyrus) and parietal regions involved in the recollection in both memory phases, and (2) prefrontal regions and the superior temporal gyrus (in the immediate and delayed memory phases respectively) involved in monitoring, evaluating and maintaining the retrieval products. These findings indicate that episodic memory retrieval depends on a network involving medial temporal lobe and frontal, parietal and temporal neocortical structures. That network was involved in immediate and delayed memory retrieval and during the course of memory consolidation, with greater activation of some nodes (mobilization of more processing resources) for the delayed respect to the immediate retrieval conditionThis study was supported by grants from the Spanish Government, Ministerio de Ciencia e Innovación (PSI2014-55316-C3-3-R; PSI2017-89389-C2-2-R), with FEDER Funds; the Galician Government, Consellería de Cultura, Educación e Ordenación Universitaria, Axudas para a Consolidación e Estruturación de Unidades de Investigación Competitivas do Sistema Universitario de Galicia: GRC (GI-1807-USC); Ref: ED431-2017/27, with FEDER fundsS

New Mediterranean Biodiversity Records (March 2017)

23 pages, 26 figures, 3 tablesThis Collective Article presents information on 22 species belonging to 5 Phyla, arranged geographically from west to east. The new records were found in 8 countries as follows: Spain: first record of the two scarcely known nemerteans Baseodiscus delineatus and Notospermus geniculatus in Formentera; Malta: second record of the alien fish Lagocephalus sceleratus; Italy: the alien polychaete Syllis pectinans and the isopod Paranthura japonica, as well as the cryptogenic opisthobranch Anteaeolidiella lurana, were found in the fouling assemblages along the docks of the port of Livorno. New decapod records are reported from Sicily (the alien Callinectes sapidus and the native Pachygrapsus maurus) and Apulia (Percnon gibbesi and Procambarus clarkii); the lesser amberjack Seriola fasciata extended its geographical range to the Egadi Isands and Siganus luridus was documented for the first time along the Ionian coasts of Apulia and Calabria. Slovenia: the first record of the alien bivalve Brachidontes pharaonis is reported, together with a survey of up to date Adriatic records. Greece: the first record of the gastropod Rhinoclavis kochi is reported from Gavdos island. In addition, two records of endangered and rare cartilaginous fish were reported, namely, the shark Hexanchus griseus and the ray Leucoraja fullonica, as well as additional records of Siganus luridus for Lesvos and Malleus regula and Fulvia fragilis from Astypalaia. Turkey: the black wing flyingfish Hirundichthys rondeletii is reported for the very first time from the Black Sea. Egypt: the Indo-Pacific crab Halimede ochtodes is reported as established in Port Said. In addition, biometric parameters and meristic counts are reported for Anthias anthias in Damietta. Cyprus: the alien opisthobranch gastropod BursatellaPeer Reviewe

A phylum-wide survey reveals multiple independent gains of head regeneration in nemertea

Animals vary widely in their ability to regenerate, suggesting that regenerative ability has a rich evolutionary history. However, our understanding of this history remains limited because regenerative ability has only been evaluated in a tiny fraction of species. Available comparative regeneration studies have identified losses of regenerative ability, yet clear documentation of gains is lacking. We assessed ability to regenerate heads and tails either through our own experiments or from literature reports for 35 species of Nemertea spanning the diversity of the phylum, including representatives of 10 families and all three orders. We generated a phylogenetic framework using sequence data to reconstruct the evolutionary history of head and tail regenerative ability across the phylum and found that all evaluated species can remake a posterior end but surprisingly few could regenerate a complete head. Our analysis reconstructs a nemertean ancestor unable to regenerate a head and indicates independent gains of head regenerative ability in at least four separate lineages, with one of these gains taking place as recently as the last 10-15 Myr. Our study highlights nemerteans as a valuable group for studying evolution of regeneration and identifying mechanisms associated with repeated gains of regenerative ability.Fil: Zattara, Eduardo Enrique. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto de Investigaciones en Biodiversidad y Medioambiente. Universidad Nacional del Comahue. Centro Regional Universidad Bariloche. Instituto de Investigaciones en Biodiversidad y Medioambiente; Argentina. National Museum of Natural History; Estados Unidos. University of Maryland; Estados UnidosFil: Fernández Álvarez, Fernando Ángel. Consejo Superior de Investigaciones Científicas. Instituto de Ciencias del Mar; EspañaFil: Hiebert, Terra. University of Oregon; Estados UnidosFil: Bely, Alexandra. University of Maryland; Estados UnidosFil: Norenburg, Jon L.. National Museum of Natural History; Estados Unido

OTP-PRL: an app for occupational risk prevention in policing activities

Background: The rapid progress in information and communication technologies has promoted the development of smartphone applications (apps) for a wide variety of purposes including workplace safety. However, no specific apps for occupational risk prevention in the police domain seemingly exist as yet. In this work, an app running under the iOS and Android operating systems was developed to help police officers become acquainted with policing-related occupational risks and to prevent their damaging consequences. Results: The proposed app, which uses an iterative user-centered design to avoid occupational risks in policing activities, was assessed for performance by a group of users and experts through a System Usability Scale (SUS) questionnaire. The mean overall score for the questionnaire was 82.3. The app has backend support to facilitate continual improvement through contributions from users and administrators. A field test revealed increased awareness of policing occupational risks after using the app in many users. Conclusions: A novel product that covers the needs of ORP requirements of police officers has been developed

Targeted bacterial conjugation mediated by synthetic cell-to-cell adhesions

Genetic interventions on microbiomes, for clinical or biotechnological purposes, remain challenging. Conjugation-based delivery of genetic cargo is still unspecific and limited by low conjugation rates. Here we report an approach to overcome these problems, based on a synthetic bacterial adhesion system. Mating assemblers consist on a synthetic adhesion formed by the expression on the surface of donor and target cells of specific nanobodies (Nb) and their cognate antigen (Ag). The Nb?Ag bridge increased 1?3 logs transfer of a variety of plasmids, especially in liquid media, confirming that cell-cell docking is a main determinant limiting mating efficiency. Synthetic cell-to-cell adhesion allows efficient conjugation to targeted recipients, enhancing delivery of desired genes to a predefined subset of prey species, or even specific pathogenic strains such as enterohemorrhagic Escherichia coli (EHEC), within a bacterial community. The synthetic conjugation enhancer presented here optimizes plasmid delivery by selecting the target hosts with high selectivity.FUNDING: Spanish Science and Innovation Ministry (MCIN) [PID2020-117923GB-I00 to F.d.l.C.]; MCIN/AEI and FEDER [BIO2017-89081-R]; MCIN/AEI and NextGeneration EU/PRTR [PLEC2021-007739 to L.A.F.]. Conflict of interest statement. None declared ACKNOWLEDGEMENTS: We thank R. Fernandez-López for providing us with the BWmKate2 strain and, along with M. P. Garcillan-Barcia, for helpful discussions. We also thank E. Zechner for providing the pAR106 plasmid, and I. Rosenshine for EHEC strains and V. Campa for technical assistance in setting up the microscopy assay

Estilo personal del terapeuta y dirección de intereses

El objetivo principal del presente estudio es profundizar en el conocimiento del Estilo Personal del Terapeuta (EPT) y estudiar las relaciones entre diferentes funciones de este estilo y la dirección de intereses de los terapeutas en formación. E] EPT es evaluado con el cuestionario EPT-C (Fernández Álvarez, García, LoBianco y Corbella, 2003) que distingue seis funciones que permiten dibujar el perfil personal de cada terapeuta: instruccional, atencional, expresiva, operacional e involucrativa. La dirección de interés del terapeuta es evaluada a través del cuestionario DIQ (Caine. Wijesinghe y Winter. 1981) que distingue entre una dirección interna o externa, La muestra del estudio está formada por 9] licenciados 9.9% hombres y 90,1% mujeres) que están cursando un Máster en psicoterapia. Los resultados de los análisis indican unas correlaciones moderadas y significativas entre las funciones atencional (receptiva) y operativa (espontáneo) y la dirección de intereses (interna) de los participantes

Remote sensing for the Spanish forests in the 21st century: a review of advances, needs, and opportunities

[EN] Forest ecosystems provide a host of services and societal benefits, including carbon storage, habitat for fauna, recreation, and provision of wood or non-wood products. In a context of complex demands on forest resources, identifying priorities for biodiversity and carbon budgets require accurate tools with sufficient temporal frequency. Moreover, understanding long term forest dynamics is necessary for sustainable planning and management. Remote sensing (RS) is a powerful means for analysis, synthesis, and report, providing insights and contributing to inform decisions upon forest ecosystems. In this communication we review current applications of RS techniques in Spanish forests, examining possible trends, needs, and opportunities offered by RS in a forestry context. Currently, wall-to-wall optical and LiDAR data are extensively used for a wide range of applications-many times in combination-whilst radar or hyperspectral data are rarely used in the analysis of Spanish forests. Unmanned Aerial Vehicles (UAVs) carrying visible and infrared sensors are gaining ground in acquisition of data locally and at small scale, particularly for health assessments. Forest fire identification and characterization are prevalent applications at the landscape scale, whereas structural assessments are the most widespread analyses carried out at limited extents. Unparalleled opportunities are offered by the availability of diverse RS data like those provided by the European Copernicus programme and recent satellite LiDAR launches, processing capacity, and synergies with other ancillary sources to produce information of our forests. Overall, we live in times of unprecedented opportunities for monitoring forest ecosystems with a growing support from RS technologies.Part of this work was funded by the Spanish Ministry of Science, innovation and University through the project AGL2016-76769-C2-1-R "Influence of natural disturbance regimes and management on forests dynamics. structure and carbon balance (FORESTCHANGE)".Gómez, C.; Alejandro, P.; Hermosilla, T.; Montes, F.; Pascual, C.; Ruiz Fernández, LÁ.; Álvarez-Taboada, F.... (2019). Remote sensing for the Spanish forests in the 21st century: a review of advances, needs, and opportunities. Forest Systems. 28(1):1-33. https://doi.org/10.5424/fs/2019281-14221S133281Ungar S, Pearlman J, Mendenhall J, Reuter D, 2003. Overview of the Earth Observing-1 (EO-1) mission. IEEE T Geosci Remote 41: 1149−1159.Valbuena R, Mauro F, Arjonilla FJ, Manzanera JA, 2011. Comparing Airborne Laser Scanning-Imagery Fusion Methods Based on Geometric Accuracy in Forested Areas. Remote Sens Environ 115(8): 1942-1956.Valbuena R, Mauro F, Rodríguez-Solano R, Manzanera JA, 2012. Partial Least Squares for Discriminating Variance Components in GNSS Accuracy Obtained Under Scots Pine Canopies. Forest Sci 58(2): 139-153.Valbuena R, De Blas A, Martín Fernández S, Maltamo M, Nabuurs GJ, Manzanera JA, 2013a. Within-Species Benefits of Back-projecting Laser Scanner and Multispectral Sensors in Monospecific P. sylvestris Forests. Eur J Remote Sens 46: 401-416.Valbuena R, Maltamo M, Martín-Fernández S, Packalen P, Pascual C, Nabuurs G-J, 2013b. Patterns of covariance between airborne laser scanning metrics and Lorenz curve descriptors of tree size inequality. Can J Remote Sens 39(1): 18-31.Valbuena R, Packalen P, García-Abril A, Mehtätalo L, Maltamo M, 2013c. Characterizing Forest Structural Types and Shelterwood Dynamics from Lorenz-based Indicators Predicted by Airborne Laser Scanning. Can J For Res 43: 1063-1074.Valbuena R, Maltamo M, Packalen P, 2016a. Classification of Multi-Layered Forest Development Classes from Low-Density National Airborne LiDAR Datasets. Forestry 89: 392-341.Valbuena R, Maltamo M, Packalen P, 2016b. Classification of Forest Development Stages from National Low-Density LiDAR Datasets: a Comparison of Machine Learning Methods. Revista de Teledetección 45: 15-25.Valbuena R, Hernando A, Manzanera JA, Martínez-Falero E, García-Abril A, Mola-Yudego B, 2017a. Most Similar Neighbour Imputation of Forest Attributes Using Metrics Derived from Combined Airborne LIDAR and Multispectral Sensors. Int J Digit Earth 11 (12): 1205-1218.Valbuena R, Hernando A, Manzanera JA, Görgens EB, Almeida DRA, Mauro F, García-Abril A, Coomes DA, 2017b. Enhancing of accuracy assessment for forest above-ground biomass estimates obtained from remote sensing via hypothesis testing and overfitting evaluation. Eco Mod 622: 15-26.Valbuena-Rabadán M, Santamaría-Pe-a J, Sanz-Adán F, 2016. Estimation of diameter and height of individual trees for Pinus sylvestris L. based on the individualising of crowns using airborne LiDAR and the National Forest Inventory data. For Sys 25(1): e046Varo-Martínez MA, Navarro-Cerrillo RM, Hernández-Clemente R, Duque-Lazo J, 2017. Semi-automated stand delineation in Mediterranean Pinus sylvestris plantations through segmentation of LiDAR data: The influence of pulse density. Int J Appl Earth Obs 56: 54-64.Vázquez de la Cueva A, 2008. Structural attributes of three forest types in central Spain and Landsat ETM+ information evaluated with redundancy analysis. Int J Remote Sens 29: 5657-5676.Verdú F, Salas J, 2010. Cartografía de áreas quemadas mediante análisis visual de imágenes de satélite en la Espa-a peninsular para el periodo 1991–2005. Geofocus 10: 54–81.Viana-Soto A, Aguado I, Martínez S, 2017. Assessment of post-fire vegetation recovery using fire severity and geographical data in the Mediterranean region (Spain). 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Natural Resources Canada, Victoria, BC, Canada. 89 pp.Wulder MA, Masek JG, Cohen WB, Loveland TR, Woodcock CE, 2012. Opening the archive: how free data has enabled the science and monitoring promise of Landsat. Remote Sens Environ 122: 2-10.Wulder MA, Hilker T, White JC, Coops NC, Masek JG, Pflugmacher D, Crevier Y, 2015. Virtual constellations for global terrestrial monitoring. Remote Sens Environ 170: 62-76.Wulder MA, White JC, Loveland TR, Woodcock CE, Belward AS, Cohen WB, Fosnight EA, Shaw J, Masek JG, Roy DP, 2016. The global Landsat archive: Status, consolidation, and direction. Remote Sens Environ 185: 271-283.Xie Q, Zhu J, Wang Ch, Fu H, López-Sánchez JM, Ballester-Berman JD, 2017. A modified dual-baseline PolInSAR method for forest height estimation. Remote Sens-Basel 9 (8): 819.Xie Y, Sha Z, Yu M, 2008. Remote sensing imagery in vegetation mapping: a review. J Plant Ecol 1 (1): 9-23.Zald HSJ, Wulder MA, White JC, Hilker T, Hermosilla T, Hobart GW, Coops NC, 2016. Integrating Landsat pixel composites and change metrics with LiDAR plots to predictively map forest structure and aboveground biomass in Saskatchewan, Canada. Remote Sens Environ 176: 188-201.Zarco-Tejada PJ, Diaz-Varela R, Angileri V, Loudjani P, 2014. Tree height quantification using very high resolution imagery acquired from an unmanned aerial vehicle (UAV) and automatic 3D photo-reconstruction methods. Eur J Agron 55: 89-99.Zarco-Tejada PJ, Hornero A, Hernández-Clemente R, Beck PSA, 2018. Understanding the temporal dimension of the red-edge spectral region for forest decline detection using high-resolution hyperspectral and Sentinel-2A imagery. ISPRS J Photogramm 137: 134-148