El panorama audiovisual en República Dominicana: la industria cinematográfica

[ES] República Dominicana, primer asentamiento del Nuevo Mundo, es un país que goza de una gran riqueza natural, histórica y cultural. Sin embargo, también enfrenta enormes retos sociales y dificultades durante un lento desarrollo que, paralelamente, va quedando reflejado en sus producciones audiovisuales. El presente trabajo tiene como objetivo principal dar a conocer la industria del cine dominicano y sus características generales, así como evaluar las fortalezas y debilidades que presenta en la actualidad. Para realizar este discurso, en primer lugar, se elabora una contextualización socioeconómica del país en general y del sector de entretenimiento en específico, y más tarde se exponen las producciones más relevantes dentro de la trayectoria cinematográfica dominicana. Mediante esta dinámica y a través de gráficos informativos, en las siguientes páginas se muestran desde las oportunidades ofrecidas por organismos e instituciones hasta la descompensación de género comprendida en el registro artístico. Así pues, para la consecución de las metas, se emplean tanto recursos bibliográficos y electrónicos como material audiovisual y entrevistas a referentes femeninos del medio. Finalmente, con la información y conocimientos obtenidos, se elaboran una serie de conclusiones y críticas constructivas sobre las necesidades de evolución en éste sector.[EN] Dominican Republic, first settlement of the new world, is a country that enjoys a great natural, historical and cultural wealth. However, it also faces enormous social challenges and difficulties during a slow development that, at the same time, is being reflected in their audiovisual productions. The present work aims to inform about the industry of the Dominican cinema and its general characteristics, as well as assess the strengths and weaknesses that currently characterize it. To make this speech, first of all, prepares a socio-economic context of the country in general and the entertainment sector in specific, and later discusses the most relevant productions of the Dominican cinematographic trajectory. Through this dynamic and informative graphics, on the following pages are exposed from the opportunities offered by agencies and institutions to the decompensation of gender in the artistic record. For the achievement of the goals, both bibliographic and electronic resources are used as well as audiovisual material and interviews with female representatives of the media. Finally, the testimonials and knowledge gained are made of a series of conclusions and constructive criticism about the needs of evolution in this sector.León Fernández-Yáñez, RM. (2019). El panorama audiovisual en República Dominicana: la industria cinematográfica. Universitat Politècnica de València. http://hdl.handle.net/10251/125936TFG

Impact of heavy metals in the microalga Chlorella sorokiniana and assessment of its potential use in cadmium bioremediation

The chlorophyte microalga Chlorella sorokiniana was tested for the bioremediation of heavy metals pollution. It was cultured with different concentrations of Cu2+, Cd2+, As (III) and As (V), showing a significant inhibition on its growth at concentrations of 500 µM Cu2+, 250 µM Cd2+, 750 µM AsO33- and 5 mM AsO43- or higher. Moreover, the consumption of ammonium was also studied, showing significant differences for concentrations higher than 1 mM of Cu2+ and As (III), and 5 mM of As (V). The determination of intracellular heavy metals concentration revealed that Chlorella sorokiniana is an outstanding Cd accumulator organism, able to accumulate 11,232 mg kg−1 of Cd, and removing 65% of initial concentration of this heavy metal. Finally, antioxidant enzymes, such as catalase (CAT) and ascorbate peroxidase (APX), and enzymes involved in the production of glutamate and cysteine, such as glutamine syntethase (GS), glutamate dehydrogenase (GDH), O-acetylserine (thiol) lyase (OASTL) and NAD-isocitrate dehydrogenase (NAD-IDH) were studied both at gene expression and enzymatic activity levels. These enzymes exhibited different grades of upregulation, especially in response to Cd and As stress. However, GS expression was downregulated when Chlorella sorokiniana was cultured in the presence of these heavy metals.This work was supported in part by research grants from the Euro- pean governments (INTERREG VAPOCTEP-2014-2020; 0055_ALGAR- ED_PLUS_5_E), the Operative FEDER Program-Andalucía 2014-2020, the University of Huelva (UHU-1257518), and by the European Regional Development Fund through the Agencia Estatal de Investigaci ́on grant (PID 2019-110438RB-C22

Dataset for proteomic analysis of Chlorella sorokiniana cells under cadmium stress

Cadmium is one of the most hazardous heavy metal for aquatic environments and one of the most toxic contaminants for phytoplankton. This work provides the dataset associated with the research publication “Effect of cadmium in the microalga Chlorella sorokiniana : a proteomic study” [1] . This dataset describes a proteomic approach, based on the sequential window acquisition of all theoretical fragment ion spectra mass spectrometry (SWATH-MS), derived from exposure of Chlorella sorokiniana to 250 μM Cd 2 + for 40 h, showing the proteins that are up- or downregulated. The processing of data included the identification of the Chlamydomonas reinhardtii protein sequences equivalent to the corresponding of Chlorella sorokiniana sequences obtained, which made possible to use KEGG Database. MS and MS/MS information, and quantitative data were deposited PRIDE public repository under accession number PXD015932 .This work was supported in part by research grants from the European governments (IN- TERREG VA-POCTEP- 2014-2020; 0055_ALGARED_PLUS_5_E), the Operative FEDER Program- Andalucía 2014-2020 ( UHU-1257518 ) University of Huelva and by the European Regional De- velopment Fund through the Agencia Estatal de Investigación grants ( PID 2019-110438RB-C22 and PID 2019-109785 GB-100 )

NK-lysin peptides ameliorate viral encephalopathy and retinopathy disease signs and provide partial protection against nodavirus infection in European sea bass

Antimicrobial peptides (AMP) comprise a wide range of small molecules with direct antibacterial activity and immunostimulatory role and are proposed as promising substitutes of the antibiotics. Additionally, they also exert a role against other pathogens such as viruses and fungi less evaluated. NK-lysin, a human granulysin orthologue, possess a double function, taking part in the innate immunity as AMP and also as direct effector in the cell-mediated cytotoxic (CMC) response. This molecule is suggested as a pivotal molecule involved in the defence upon nervous necrosis virus (NNV), an epizootic virus provoking serious problems in welfare and health status in Asian and Mediterranean fish destined to human consumption. Having proved that NK-lysin derived peptides (NKLPs) have a direct antiviral activity against NNV in vitro, we aimed to evaluate their potential use as a prophylactic treatment for European sea bass (Dicentrarchus labrax), one of the most susceptible cultured-fish species. Thus, intramuscular injection of synthetic NKLPs resulted in a very low transcriptional response of some innate and adaptive immune markers. However, the injection of NKLPs ameliorated disease signs and increased fish survival upon challenge with pathogenic NNV. Although NKLPs showed promising results in treatments against NNV, more efforts are needed to understand their mechanisms of action and their applicability to the aquaculture industry.This work was supported by Ministerio de Economía y Competitividad (MINECO; grant AGL2016-74866-C3-1-R with FEDER co-funds), Agencia Espanola ˜ de Investigacion ´ (PID2019-105522 GB-I00), Fundacion ´ S´eneca, Grupo de Excelencia de la Regi´ on de Murcia (grant 19883/GERM/15) and National Commission for Scientific & Technological Research Chile (grant FONDECYT N◦ 1170379)

On statistical convergence and strong Cesàro convergence by moduli

In this paper we will establish a result by Connor, Khan and Orhan (Analysis 8:47–63, 1988; Publ. Math. (Debr.) 76:77–88, 2010) in the framework of the statistical convergence and the strong Cesàro convergence defined by a modulus function f . Namely, for every modulus function f , we will prove that a f -strongly Cesàro convergent sequence is always f -statistically convergent and uniformly integrable. The converse of this result is not true even for bounded sequences. We will characterize analytically the modulus functions f for which the converse is true. We will prove that these modulus functions are those for which the statistically convergent sequences are f -statistically convergent, that is, we show that Connor–Khan–Orhan’s result is sharp in this sense

Amino acids profile of 56 species of microalgae reveals that free amino acids allow to distinguish between phylogenetic groups

Microalgae represent a phototrophic resource with a high protein content, whose nutritional value is very high as a result of its amino acid profile. Although the total amino acid profile has been repeatedly described to change little among phylogenetic groups of microalgae, some variability has occasionally been reported. Part of this uncertainty is associated with the low phylogenetic diversity encompassed in the work to date and the high methodological variability between studies. Among these studies, very few have differentiated between total and free amino acid content for common microalgae samples. Thus, in the present work, the profile of total and free amino acids has been determined in a diverse group composed of 56 species of microalgae belonging to 7 phyla. A multivariate analysis of the total amino acid profile in the studied microalgae group revealed close amino acid patterns throughout microalgae phylogeny and agreed with results obtained from similar analysis performed with published data. Conversely, the free amino acid profile strongly differentiated between phylogenetic groups. On the one hand, species of Cyanobacteria, Plantae, Cryptophyta, and Bacillariophyta showed close free amino acid patterns, characterized by the highest abundance of free glutamic acid. Ochrophyta species were particularly rich in free proline, while the free amino acid profile of Miozoa and Haptophyta species stood out from the rest of the phylogenetic groups for their outstanding levels of the two essential amino acids phenylalanine and lysine. Haptophyta species were also characterized by their much higher free tyrosine content.This work was supported by the European Union Cooperation Program INTERREG VA POCTEP-055 ALGARED PLUS5E Spain-Portugal (2014–2020). The authors are grateful to the Fertinagro Biotech Foundation (C´atedra Fertinagro Biotech Universidad de Huelva). Funding for open access charge: Universidad de Huelva / CBUA is acknowledged

Simultaneous production of carotenoids and chemical building blocks precursors from chlorophyta microalgae

Replacement of fossil fuels has to be accompanied by the incorporation of bio-based procedures for the production of fine chemicals. With this aim, the microalga Chlamydomonas reinhardtii was selected for its ability to accumulate starch, an environmentally-friendly alternative source of chemical building blocks, such as 5′ -hydroxymethylfurfural or levulinic acid. The content of appreciated lipophilic coproducts was assessed in the selected microalga cultured at different nutritional conditions; and the parameters for the acidic hydrolysis of the algal biomass, obtained after pigments extraction, were optimized using a Central Composite Design. Response Surface Methodology predicted that the optimal hydrolysis conditions were elevated temperature, high DMSO % and short hydrolysis time for glucose. LA was favored at long times and high acid % and 5′ -HMF at lower acid % and high DMSO %. Chlamydomonas can therefore be used as a sustainable feedstock for the simultaneous production of high-added value lipophilic compounds and platform chemicals.Funding for open access charge: Universidad de Huelva / CBUA

From Olive Fruits to Olive Oil: Phenolic Compound Transfer in Six Different Olive Cultivars Grown under the Same Agronomical Conditions

Phenolic compounds are responsible of the nutritional and sensory quality of extra-virgin olive oil (EVOO). The composition of phenolic compounds in EVOO is related to the initial content of phenolic compounds in the olive-fruit tissues and the activity of enzymes acting on these compounds during the industrial process to produce the oil. In this work, the phenolic composition was studied in six major cultivars grown in the same orchard under the same agronomical and environmental conditions in an effort to test the effects of cultivars on phenolic composition in fruits and oils as well as on transfer between matrices. The phenolic fractions were identified and quantified using high-performance liquid chromatography-diode array detector-time-of-flight-mass spectrometry. A total of 33 phenolic compounds were determined in the fruit samples and a total of 20 compounds in their corresponding oils. Qualitative and quantitative differences in phenolic composition were found among cultivars in both matrices, as well as regarding the transfer rate of phenolic compounds from fruits to oil. The results also varied according to the different phenolic groups evaluated, with secoiridoids registering the highest transfer rates from fruits to oils. Moreover, wide-ranging differences have been noticed between cultivars for the transfer rates of secoiridoids (4.36%–65.63% of total transfer rate) and for flavonoids (0.18%–0.67% of total transfer rate). ‘Picual’ was the cultivar that transferred secoiridoids to oil at the highest rate, whereas ‘Changlot Real’ was the cultivar that transferred flavonoids at the highest rates instead. Principal-component analysis confirmed a strong genetic effect on the basis of the phenolic profile both in the olive fruits and in the oils.This research was partly funded by project P11-AGR-7301 of the Andalusian Regional Government Council of Innovation and Science and the Spanish Ministry of Economy and Competitiveness. The analytical part was financially supported by Spanish Ministry of Education and Science (AGL2015-67995-C3) and the Andalusian Regional Government Council of Innovation and Science (P10-FQM-6563, P11-CTS-7625). The authors thank the International Olive Oil Council (IOC) for the grant (Ref.: T1 6-Doct 1/12) and the Ministry of Education and Competitiveness for the postdoctoral contract Juan de la Cierva (JCI-2012-12566)