Influence of Ground Reaction Forces and Joint Velocities on Kicking Velocity

Introduction: Kicking is a vital component in the game of soccer. One major factor that influences the success of a scoring attempt is ball velocity. Ground reaction force (GRF) and joint velocities of the lower extremities are variables of interest for increasing kicking velocity. Previous studies have shown exercise programs used to strengthen the muscles used in kicking have been successful in increasing kicking velocity (Manolopoulos, et al., 2013).[GJR1] To date, no known studies have analyzed the specific relationship between GRF, joint velocity and kicking velocity. Purpose: The goal of this pilot study was to analyze the influence of ground reaction forces and joint velocities on kicking velocity. Methods: Four female Division II collegiate soccer players [GJR2] completed 3 instep soccer kicks [GJR3] using their dominant, right leg. Their motion was captured using the Cortex 8.1 Motion Analysis Software. Ball velocity, right and left anterior superior iliac spine (ASIS) and right and left ankle velocities were calculated using the motion analysis software. Ground reaction forces from the plant leg were also measured using force plate data from the Cortex software. Bivariate Pearson correlations with 0.95 confidence intervals were computed using SPSS version 28 for the resultant velocities of the right and left ASIS, right and left ankles, and the velocity of the ball. They were also calculated for the peak GRF in the anteroposterior (X), mediolateral (Y) and vertical (Z) directions and ball velocity. A correlation value of \u3e0.800 or \u3c -0.800 was considered significant. Results: Significant correlations were found between peak resultant ball velocity and GRF in the X direction (-0.907), GRF in the Y direction (0.867), R ASIS velocity (0.950), R ankle velocity (0.855), and L ankle velocity (0.977). No significant correlations were found between peak resultant ball velocity and GRF in the Z direction (0.788), or peak resultant ball velocity and peak joint velocity of the L ASIS (0.692). Conclusion: Braking force of the planting leg is shown to correlate significantly with kicking velocity. Although high braking force allows for a faster ball, this can have other implications to injury [GJR4] (Ball, 2012; Jones & Graham-Smith, 2016). Linear velocity of the hip also allows for greater ball velocity. As a pilot study, this study lacks the statistical power to extrapolate the information to larger populations. Therefore, additional studies are needed to further investigate the relationships between kicking mechanics and the resulting ball velocity

Correlation Between Quadriceps and Hamstring Isokinetic Strength to Ball Velocity During a Soccer Kick

When kicking a soccer ball, large forces are generated by the quadriceps and hamstring muscles that extend and flex the knee. The angular acceleration[GJR1] at this joint and the torques produced are[GJR2] related. PURPOSE: The goal of this pilot study was to explore the relationship between isokinetic strength[GJR3] of the quadriceps and hamstring muscles to velocity of a kicked soccer ball and determine if isokinetic testing of quadriceps and hamstring strength can predict soccer ball velocity during a kick. Methods: Four female NCAA Division II soccer athletes completed maximal effort knee flexion and extension at three isokinetic speeds, 60°/second, 180°/second, and 300°/second using the Biodex 3 Isokinetic Dynamometer. Cortex 8.1 Motion Analysis Software was used to record three maximal kicks with the dominant leg. Bivariate Pearson correlation coefficients were calculated between both data sets using SPSS version 28. Results: Ball velocity was significantly and positively correlated with Right Leg Flexion Acceleration time at 60°/second(r= 0.860),[GJR4] Left Leg Extension Acceleration at 180°/second (r= 0.950), and Left Leg Extension Acceleration at 300°/second (r= 0.915). Two significant negative relationships were discovered between ball velocity and left leg extension acceleration at 300°/second (r= -0.950), and left angle of peak extension torque at 300°/second (r= - 0.915). Conclusion: The ability to quickly accelerate the non-kicking leg to extension combined with the ability to reach angle of peak extension torque is associated with the ability to quickly stabilize the plant leg. Flexion of the kicking leg at a lower angular velocity corresponds with a higher force production and when combined with a positive correlation to ball velocity, suggests increased loading of the kicking leg prior to ball contact. Lastly, the negative correlation between ball velocity and kicking-leg extension acceleration would suggest that faster acceleration leads to increased ball velocity. Because of this, isokinetic testing of the quadricep and hamstring strength is likely a good predictor of kicking velocity. Further testing is required to determine if present correlations are applicable to other populations of soccer athletes, which can affect training and return-to-play practices

A Community-Wide Collaboration to Reduce Cardiovascular Disease Risk: The Hearts of Sonoma County Initiative.

PURPOSE AND OBJECTIVES: Collaboration across multiple sectors is needed to bring about health system transformation, but creating effective and sustainable collaboratives is challenging. We describe outcomes and lessons learned from the Hearts of Sonoma County (HSC) initiative, a successful multi-sector collaborative effort to reduce cardiovascular disease (CVD) risk in Sonoma County, California. INTERVENTION APPROACH: HSC works in both clinical systems and communities to reduce CVD risk. The initiative grew out of a longer-term county-wide collaborative effort known as Health Action. The clinical component involves activating primary care providers around management of CVD risk factors; community activities include community health workers conducting blood pressure screenings and a local heart disease prevention campaign. EVALUATION METHODS: The impact of the clinical improvement efforts was tracked using blood pressure data from the 4 health systems participating in HSC. Descriptive information on the community-engagement efforts was obtained from program records. Lessons learned in developing and maintaining the collaborative were gathered through document review and interviews with key informants. RESULTS: Favorable trends were seen in blood pressure control among patients with hypertension in the participating health systems: patients with controlled blood pressure increased from 58% in 2014 to 67% in 2016 (P \u3c .001). Between 2017 and 2019, the community engagement effort conducted 99 outreach events, reaching 1,751 individuals, and conducted 1,729 blood pressure screenings, with 441 individuals referred to clinical providers for follow-up care. HSC scored highly on 6 essential elements of an effective coalition and achieved a degree of sustainability that has eluded many other collaboratives. IMPLICATIONS FOR PUBLIC HEALTH: Factors contributing to the success of HSC include 1) starting small and focused to build trust among participants and demonstrate value, 2) working within the framework of a larger effort, and 3) providing long-term, open-ended backbone support

The rhesus macaque is three times as diverse but more closely equivalent in damaging coding variation as compared to the human

Abstract Background As a model organism in biomedicine, the rhesus macaque (Macaca mulatta) is the most widely used nonhuman primate. Although a draft genome sequence was completed in 2007, there has been no systematic genome-wide comparison of genetic variation of this species to humans. Comparative analysis of functional and nonfunctional diversity in this highly abundant and adaptable non-human primate could inform its use as a model for human biology, and could reveal how variation in population history and size alters patterns and levels of sequence variation in primates. Results We sequenced the mRNA transcriptome and H3K4me3-marked DNA regions in hippocampus from 14 humans and 14 rhesus macaques. Using equivalent methodology and sampling spaces, we identified 462,802 macaque SNPs, most of which were novel and disproportionately located in the functionally important genomic regions we had targeted in the sequencing. At least one SNP was identified in each of 16,797 annotated macaque genes. Accuracy of macaque SNP identification was conservatively estimated to be >90%. Comparative analyses using SNPs equivalently identified in the two species revealed that rhesus macaque has approximately three times higher SNP density and average nucleotide diversity as compared to the human. Based on this level of diversity, the effective population size of the rhesus macaque is approximately 80,000 which contrasts with an effective population size of less than 10,000 for humans. Across five categories of genomic regions, intergenic regions had the highest SNP density and average nucleotide diversity and CDS (coding sequences) the lowest, in both humans and macaques. Although there are more coding SNPs (cSNPs) per individual in macaques than in humans, the ratio of dN/dS is significantly lower in the macaque. Furthermore, the number of damaging nonsynonymous cSNPs (have damaging effects on protein functions from PolyPhen-2 prediction) in the macaque is more closely equivalent to that of the human. Conclusions This large panel of newly identified macaque SNPs enriched for functionally significant regions considerably expands our knowledge of genetic variation in the rhesus macaque. Comparative analysis reveals that this widespread, highly adaptable species is approximately three times as diverse as the human but more closely equivalent in damaging variation.http://deepblue.lib.umich.edu/bitstream/2027.42/112453/1/12863_2011_Article_1004.pd

P120-Catenin Isoforms 1 and 3 Regulate Proliferation and Cell Cycle of Lung Cancer Cells via β-Catenin and Kaiso Respectively

<div><h3>Background</h3><p>The different mechanisms involved in p120-catenin (p120ctn) isoforms' 1/3 regulation of cell cycle progression are still not elucidated to date.</p> <h3>Methods and Findings</h3><p>We found that both cyclin D1 and cyclin E could be effectively restored by restitution of p120ctn-1A or p120ctn-3A in p120ctn depleted lung cancer cells. When the expression of cyclin D1 was blocked by co-transfection with siRNA-cyclin D1 in p120ctn depleted cells restoring p120ctn-1A or 3A, the expression of cyclin E was slightly decreased, not increased, implying that p120ctn isoforms 1 and 3 cannot up-regulate cyclin E directly but may do so through up-regulation of cyclin D1. Interestingly, overexpression of p120ctn-1A increased β-catenin and cyclin D1 expression, while co-transfection with siRNA targeting β-catenin abolishes the effect of p120ctn-1A on up-regulation of cyclin D1, suggesting a role of β-catenin in mediating p120ctn-1A's regulatory function on cyclin D1 expression. On the other hand, overexpression of p120ctn isoform 3A reduced nuclear Kaiso localization, thus decreasing the binding of Kaiso to KBS on the cyclin D1 promoter and thereby enhancing the expression of cyclin D1 gene by relieving the repressor effect of Kaiso. Because overexpressing NLS-p120ctn-3A (p120ctn-3A nuclear target localization plasmids) or inhibiting nuclear export of p120ctn-3 by Leptomycin B (LMB) caused translocation of Kaiso to the nucleus, it is plausible that the nuclear export of Kaiso is p120ctn-3-dependent.</p> <h3>Conclusions</h3><p>Our results suggest that p120ctn isoforms 1 and 3 up-regulate cyclin D1, and thereby cyclin E, resulting in the promotion of cell proliferation and cell cycle progression in lung cancer cells probably via different protein mediators, namely, β-catenin for isoform 1 and Kaiso, a negative transcriptional factor of cyclin D1, for isoform 3.</p> </div

Fine-mapping of the HNF1B multicancer locus identifies candidate variants that mediate endometrial cancer risk.

Common variants in the hepatocyte nuclear factor 1 homeobox B (HNF1B) gene are associated with the risk of Type II diabetes and multiple cancers. Evidence to date indicates that cancer risk may be mediated via genetic or epigenetic effects on HNF1B gene expression. We previously found single-nucleotide polymorphisms (SNPs) at the HNF1B locus to be associated with endometrial cancer, and now report extensive fine-mapping and in silico and laboratory analyses of this locus. Analysis of 1184 genotyped and imputed SNPs in 6608 Caucasian cases and 37 925 controls, and 895 Asian cases and 1968 controls, revealed the best signal of association for SNP rs11263763 (P = 8.4 × 10(-14), odds ratio = 0.86, 95% confidence interval = 0.82-0.89), located within HNF1B intron 1. Haplotype analysis and conditional analyses provide no evidence of further independent endometrial cancer risk variants at this locus. SNP rs11263763 genotype was associated with HNF1B mRNA expression but not with HNF1B methylation in endometrial tumor samples from The Cancer Genome Atlas. Genetic analyses prioritized rs11263763 and four other SNPs in high-to-moderate linkage disequilibrium as the most likely causal SNPs. Three of these SNPs map to the extended HNF1B promoter based on chromatin marks extending from the minimal promoter region. Reporter assays demonstrated that this extended region reduces activity in combination with the minimal HNF1B promoter, and that the minor alleles of rs11263763 or rs8064454 are associated with decreased HNF1B promoter activity. Our findings provide evidence for a single signal associated with endometrial cancer risk at the HNF1B locus, and that risk is likely mediated via altered HNF1B gene expression

Recruiting women with ductal carcinoma in situ to a randomised controlled trial: lessons from the LORIS study

BackgroundThe LOw RISk DCIS (LORIS) study was set up to compare conventional surgical treatment with active monitoring in women with ductal carcinoma in situ (DCIS). Recruitment to trials with a surveillance arm is known to be challenging, so strategies to maximise patient recruitment, aimed at both patients and recruiting centres, were implemented.MethodsWomen aged ≥ 46 years with a histologically confirmed diagnosis of non-high-grade DCIS were eligible for 1:1 randomisation to either surgery or active monitoring. Prior to randomisation, all eligible women were invited to complete: (1) the Clinical Trials Questionnaire (CTQ) examining reasons for or against participation, and (2) interviews exploring in depth opinions about the study information sheets and film. Women agreeing to randomisation completed validated questionnaires assessing health status, physical and mental health, and anxiety levels. Hospital site staff were invited to communication workshops and refresher site initiation visits to support recruitment. Their perspectives on LORIS recruitment were collected via surveys and interviews.ResultsEighty percent (181/227) of eligible women agreed to be randomised. Over 40% of participants had high anxiety levels at baseline. On the CTQ, the most frequent most important reasons for accepting randomisation were altruism and belief that the trial offered the best treatment, whilst worries about randomisation and the influences of others were the most frequent most important reasons for declining. Most women found the study information provided clear and useful. Communication workshops for site staff improved knowledge and confidence but only about half said they themselves would join LORIS if eligible. The most common recruitment barriers identified by staff were low numbers of eligible patients and patient preference.ConclusionsRecruitment to LORIS was challenging despite strategies aimed at both patients and site staff. Ensuring that recruiting staff support the study could improve recruitment in similar future trials