Evaluation in a dog model of three antimicrobial glassy coatings: Prevention of bone loss around implants and microbial assessments

This is an open access article distributed under the terms of the Creative Commons Attribution License.-- et al.[Objectives]: The aim of the present study is to evaluate, in a ligature-induced peri-implantitis model, the efficacy of three antimicrobial glassy coatings in the prevention of biofilm formation, intrasulcular bacterial growth and the resulting peri-implant bone loss. [Methods]: Mandibular premolars were bilaterally extracted from five beagle dogs. Four dental implants were inserted on each hemiarch. Eight weeks after, one control zirconia abutment and three with different bactericidal coatings (G1n-Ag, ZnO35, G3) were connected. After a plaque control period, bacterial accumulation was allowed and biofilm formation on abutments was observed by Scanning Electron Microscopy (SEM). Peri-implantitis was induced by cotton ligatures. Microbial samples and peri-implant crestal bone levels of all implant sites were obtained before, during and after the breakdown period. [Results]: During experimental induce peri-implantitis: colony forming units counts from intrasulcular microbial samples at implants with G1n-Ag coated abutment remained close to the basal inoculum; G3 and ZnO35 coatings showed similar low counts; and anaerobic bacterias counts at control abutments exhibited a logarithmic increase by more than 2. Bone loss during passive breakdown period was no statistically significant. Additional bone loss occurred during ligature-induce breakdown: 0.71 (SD 0.48) at G3 coating, 0.57 (SD 0.36) at ZnO35 coating, 0.74 (SD 0.47) at G1n-Ag coating, and 1.29 (SD 0.45) at control abutments; and statistically significant differences (p<0.001) were found. The lowest bone loss at the end of the experiment was exhibited by implants dressing G3 coated abutments (mean 2.1; SD 0.42). [Significance]: Antimicrobial glassy coatings could be a useful tool to ward off, diminish or delay periimplantitis progression.This work was supported by the Spanish Ministry of Science and Innovation (MICINN) and The Spanish National Research Council (CSIC) under the projects MAT2012-38645 and 201360E012 respectively. B. Cabal acknowledges financial support from JAE-Doc program (CSIC, cofounded by FSE).Peer Reviewe

Inhibitory effect on in vitro Streptococcus oralis biofilm of a soda-lime glass containing silver nanoparticles coating on titanium alloy

This is an open-access article.-- et al.This paper reports the effect of soda-lime-glass-nAg coating on the viability of an in vitro biofilm of Streptococcus oralis. Three strains (ATCC 35037 and two clinical isolates from periodontitis patients) were grown on coated with glass, glass containing silver nanoparticles, and uncoated titanium alloy disks. Two different methods were used to quantify biofilm formation abilities: crystal violet staining and determination of viable counts. The influence of the surface morphology on the cell attachment was studied. The surface morphology was characterized by scanning electron microscopy (SEM) and using a profilometer. SEM was also used to study the formation and the development of biofilm on the coated and uncoated disks. At least a >99.7% inocula reduction of biofilm respect to titanium disks and also to glass coated disks was observed in the glass-nAg coated disks for all the studied strains. A quantitative evaluation of the release of silver was conducted in vitro to test whether and to what extend the biocidal agent (silver) could leach from the coating. These findings suggest that the biofilm formation of S. oralis strains is highly inhibited by the glass-nAg and may be useful for materials which require durable antibacterial effect on their surfaces, as it is the case of dental implants. © 2012 Cabal et al.This work was supported by the Spanish Ministry of Science and Innovation under the project MAT2009-14542-C02-01.Peer Reviewe

Re-evaluating the Neolithic: The Impact and the Consolidation of Farming Practices in the Cantabrian Region (Northern Spain)

Abstract:Research projects undertaken in the Cantabrian region since 1980 have produced new, high-quality information about the neolithisation process(es) in this area. It is now necessary to review this archaeological information and test the main hypotheses put forward to explain it. This paper presents an update on the archaeological evidence (sites, chronological dates, archaeozoological, archaeobotanical and technological information) for the early Neolithic in the Cantabrian region. It summarizes recent research on neolithisation in the region, and assesses the impact of this process during the early Neolithic, and its later consolidation. Although the available information is still incomplete, it is now possible to identify the focal point of the introduction of elements characteristic of the Neolithic way of life in the region. Current evidence suggests that it is in the eastern sector, where the earliest arrival of domesticates and new technologies such as pottery has been attested. The existence of continuities?such as sustained reliance on hunting and gathering and the coexistence of old and new funerary rites?suggests the persistence of native populations, which gradually participated in the neolithisation process after an ?availability phase?

La arquitectura defensiva y doméstica del castro del Alto de La Garma (Cantabria)

RESUMEN: El castro del Alto de La Garma, en Omoño, en Ribamontán al Monte, es un pequeño recinto fortificado de la Primera Edad del Hierro situado en la zona costera de Cantabria. Las excavaciones realizadas han permitido documentar una ocupación en dos fases. La primera, que se enmarca en la Edad del Hierro i, cuenta con una muralla defensiva construida en terrazas y viviendas circulares, cuya cronología puede situarse entre finales del s. VIII a. C. y finales del s. VI a. C. La segunda fase, tras una centuria de abandono, se fecha entre el final del s. V y el IV a. C., se caracteriza por la construcción de una muralla de doble careado. Algunos materiales arqueológicos y la datación absoluta abren la posibilidad de que esta fortificación, tras un nuevo abandono, hubiera sido utilizada durante la Segunda Edad del Hierro o durante la etapa romana. En este artículo se analizan sus ocupaciones a partir del estudio de la arquitectura defensiva y doméstica, poniéndose en relación con otros yacimientos de la Edad del Hierro de la región cantábrica.ABSTRACT: The archaeological site of Alto de La Garma, in Omoño, Ribamontán al Monte, is an Early Iron Age little hillfort located in the coastal area of Cantabria. The excavations carried out have documented a settlement with two occupation phases. The first, which dates from the Iron Age i, has a defensive wall built in terraces and circular houses, whose chronology is between the end of the 8th century bc and the end of the 6th century bc. The second phase, after a century of abandonment, dates between the end of the 5th and the 4th century bc, is characterized by the construction of a double-faced wall. Some archaeological materials and absolute dating also open the possibility that the hillfort, after a new abandonment, was in use during the Second Iron Age or the Roman Age. In this paper we present a study of all their occupations focusing on the analysis of its defensive and domestic architecture, in relation to others archaeological sites from the Iron Age of the Cantabrian Region

Histological response of soda-lime glass-ceramic bactericidal rods implanted in the jaws of beagle dogs

Bacterial and fungal infections remain a major clinical challenge. Implant infections very often require complicated revision procedures that are troublesome to patients and costly to the healthcare system. Innovative approaches to tackle infections are urgently needed. We investigated the histological response of novel free P2O5 glass-ceramic rods implanted in the jaws of beagle dogs. Due to the particular percolated morphology of this glass-ceramic, the dissolution of the rods in the animal body environment and the immature bone formation during the fourth months of implantation maintained the integrity of the glass-ceramic rod. No clinical signs of inflammation took place in any of the beagle dogs during the four months of implantation. This new glass-ceramic biomaterial with inherent bactericidal and fungicidal properties can be considered as an appealing candidate for bone tissue engineeringThis work was supported by the Spanish Ministry of Science and Innovation (MICINN) under the projects MAT2012-38645. A.P. Tomsia work was supported by the National Institutes of Health/National Institute of Dental and Craniofacial Research (NIH/NIDCR) Grant No. 1R01DE015633S

Contribution to the study of the Alto de La Garma hillfort (Cantabria): the pottery of the Early Iron Age

RESUMEN: El castro del Alto de La Garma (Omoño, Ribamontán al Monte) es un pequeño recinto fortificado de la Primera Edad del Hierro situado en la zona costera de Cantabria. Las excavaciones realizadas han permitido documentar una fortificación datada en el s. vii a. C., reformada en un momento posterior, en cuyo interior se levantaron estructuras habitacionales de planta circular. Dentro del registro material recuperado destaca el conjunto cerámico sobre el que se centra el siguiente trabajo. En este artículo se presenta el análisis macroscópico del material cerámico atendiendo a sus rasgos tecnológicos, morfológicos y decorativos. Esto permitirá abordar desde bases más sólidas futuros estudios de este tipo de materiales en otros yacimientos de la región. Finalmente, se presenta una comparación del conjunto con el documentado en otros yacimientos coetáneos en la zona, destacando las similitudes o diferencias entre los materiales recuperados.ABSTRACT: The archaeological site of Alto de La Garma (Omoño, Ribamontán al Monte, Spain) is an early Iron Age hillfort located in the littoral of Cantabria. The excavations have documented a fortification dated to the viith century bc, rebuilt at a later time. Inside the fortified area, some circular huts have been found. This paper presents the macroscopic analysis of the pottery ensemble focused on the technological, morphological and decorative features. That will provide a solid ground for future studies of this type of material in other sites of the region. To finish, it presents a comparison of this ensemble with the remains recorded in others contemporary archaeological sites of the area, highlighting the similarities and differences between them

Geoarchaeological and Chronostratigraphic study of the Upper Pleistocene record of the Cierro cave (Fresno, Ribadesella, Asturias, Spain)

Resumen: La cueva de El Cierro (Fresno, Ribadesella, Asturias) es una cavidad kárstica situada en la margen oeste del río Sella y desarrollada en las calizas carboníferas del Macizo Asturiano de la Cordillera Cantábrica (norte de la Península Ibérica), que contiene un importante registro sedimentario, arqueológico y paleontológico del Pleistoceno superior y Holoceno inferior, con abundantes evidencias tecnológicas y restos faunísticos. El registro arqueológico arranca con un nivel que podría corresponder al Paleolítico medio final, sigue con una completa secuencia del Paleolítico superior de la que se cuenta con un nivel del Magdaleniense inferior claramente identificado y datado, y termina con unos niveles datados a finales del Paleolítico superior/Aziliense y en el Mesolítico que configuran un conchero. La secuencia estratigráfica se compone de catorce niveles agrupados en dos unidades litoestratigráficas con características sedimentarias muy distintas, una inferior, siliciclástica y otra superior biogénica formada por la acumulación de conchas y huesos de aporte antrópico, que se estudian con metodología geoarqueológica. En este trabajo se analiza la secuencia litoestratigráfica y se presentan los datos de los análisis granulométricos, mineralógicos, edafológicos y radiométricos. El resultado de estos análisis permite interpretar con precisión tanto la litoestratigrafía del depósito como los procesos sedimentarios y diagenéticos responsables de su formación y posterior evolución. Las dataciones radiocarbónicas disponibles permiten situar los niveles superiores de la secuencia siliciclástica en el GS 2b y el conchero inferior en el GI 1 y el GS 1 (Dryas reciente), al final del Pleistoceno superior, mientras que el conchero superior estaría situado en la cronozona Boreal en los inicios del Holoceno.Abstract: The Cierro Cave (Fresno, Ribadesella, Asturias) is a karst cavity located in the west bank of the Sella river anddeveloped in the Carboniferous limestone of the Asturian Massif of the Cantabrian Range (northern Iberian Peninsula). This cave contains an important sedimentary, archaeological and palaeontological record of the Upper Pleistocene and Early Holocene with abundant technological remains (lithic and bone industries) and bone remains of mammals and others vertebrates. The archaeological record starts with a probably Middle Palaeolithic level, followed by a complete sequence of the Upper Paleolithic, with a Lower Magdalenian level clearly identified and dated, and ends with levels of the Late Upper Magdalenian/Azilian and the Mesolithic forming three shell middens. The stratigraphic sequence consists of fourteen levels grouped in two litostratigraphic units with very different sedimentary characteristics studied by geoarchaeological methods. The lower unit is characterized by the significant presence of siliceous sands and silts while the upper unit is formed by three anthropic shell middens cemented by carbonates. This paper analyzes the lithostratigraphic sequence and shows the results of the granulometric, mineralogical, edaphic and radiometric analysis. These results allows us to interpret precisely the lithostratigraphy of the deposits and the sedimentary and diagenetic processes responsible for their formation and evolution. In addition, the radiocarbon dates obtained allow us to place the upper levels of the clastic sequence in the GS 2b and the lower shell midden in GI 1 and GS 1 at the end of the Upper Pleistocene, while the upper shell midden would be located in the Boreal cronozona in the Early Holocene.Este trabajo ha sido financiado por el Plan Nacional de I+D+I del Ministerio de Economía y Competitividad en el marco de los proyectos de investigación HAR2011-29907-\ud C03-03/HIST (investigador principal: Dr. Esteban Álvarez- Fernández) y HAR2011-29907-C03-00/HIST (investigador principal: Dr. Pablo Arias Cabal)

Tito Bustillo Cave (Ardines, Ribadesella, Asturias, Spain): archaeological excavations in the Living Area

RESUMEN: En este artículo se revisa la información disponible sobre las intervenciones arqueológicas llevadas a cabo en la conocida como Área de Estancia de la cueva de Tito Bustillo, tanto por Miguel Ángel García Guinea (campaña de 1970), como por J. Alfonso Moure Romanillo (campañas de 1972 a 1986). Se hace particular hincapié en la publicación de nuevos datos sobre el yacimiento, relacionados con dataciones radiocarbónicas y con determinados análisis llevados a cabo a partir de los restos de origen biótico (mamíferos, aves, peces, moluscos, industria ósea y objetos de adorno) y abiótico (procedencia de las materias primas líticas). Por último, se adelantan los primeros resultados sobre la intervención arqueológica llevada a cabo en el año 2020 en esta zona de la cueva, que consistió en la realización de la topografía y en la limpieza de los perfiles dejados en las intervenciones de los años setenta y ochenta del siglo pasado, con el objeto de tomar muestras de diferentes tipos, destinadas a comprender mejor la dinámica del yacimiento.ABSTRACT: This paper reviews the available information about the excavations carried out by Miguel Ángel García Guinea (1970) and J. Alfonso Moure Romanillo (1972-1986) in the chamber in Tito Bustillo cave known as the Living Area. It highlights the publication of new data about the deposit, comprising radiocarbon dates and the analyses of biotic remains (large mammals, birds, fish, molluscs, bone industry and objects of adornment) and abiotic artefacts (provenance of lithic raw materials). It also provides the preliminary results of the archaeological fieldwork in the same part of the cave in 2020, which consisted of surveying the area and cleaning up the sections left by the excavations in the 1970s and 80s, in order to collect different types of samples and acquire a better understanding of the dynamic of the deposit.Este artículo ha sido realizado dentro de dos proyectos. En primer lugar, del proyecto Símbolos subterráneos: una aproximación al pensamiento de los cazadores-recolectores del Tardiglaciar y del Holoceno usando tecnologías informáticas (SimTIC) (HAR2017-82557-P), financiado por el Ministerio de Economía, Industria y Competitividad. Gobierno de España; en segundo lugar, Contactos a corta, media y larga distancia entre los grupos humanos del Paleolítico superior en el Golfo de Bizkaia a través de los recursos bióticos y abióticos (PaleontheMove) (PID2020-114462GB-I00), financiado por el Ministerio de Ciencia e Innovación. Gobierno de España