Feasibility of abstinence as a preventive strategy for HIV/AIDS control in the University student community in Kumasi, Ghana.

HIV is spreading and the youth bear the brunt of its onslaught. Though abstinence until marriage is thought to be the most effective method of HIV prevention for the youth, others think it is ineffective. This study assessed the feasibility of abstinence in preventing HIV/AIDS spread among tertiary students of the KNUST. Study type was non- interventional, descriptive and design current cross- sectional. Study participants were selected by stratified sampling, followed by systematic sampling. A total of 300 participants were sampled. Seventy nine (79%) (95% CI, 73.9-83.8) said STIs could be avoided by abstaining from sex. Ninety six (96%) (95% CI, 93.5-98.3%) said HIV could be acquired via sex. Ninety six (96%) (95% CI, 93.8- 98.5%) of those who said HIV could be avoided said it could be done by abstaining from sex. Seventy two (72%) were of the view that sex should start only after marriage. Sixty nine 69% (95% CI, 63.3-74.4%) said they would wait till after marriage to involve in sex. Sixty seven (67%) (95% CI, 60.7- 72.1%) were encouraged by peers to abstain from sex, Seventy four (74%) (95% CI, 68.5- 79.1%) thought colleagues their age had premarital sex and 28% (95% CI, 22.5- 33.4%) said they were pressured to have sex. Thirty one (31%) (95% CI, 25.6- 36.7%) of respondents were sexually experienced. There is the general view that HIV/AIDS spread among the youth can be reduced by abstaining from sex until marriage and that abstinence could and should be encouraged as a preventive strategy for HIV/AIDS.Journal of Science and Technology (Ghana) Vol. 27 (2) 2007: pp. 1-

An improved predictive recognition model for Cys2-His2 zinc finger proteins

Cys2-His2 zinc finger proteins (ZFPs) are the largest family of transcription factors in higher metazoans. They also represent the most diverse family with regards to the composition of their recognition sequences. Although there are a number of ZFPs with characterized DNA-binding preferences, the specificity of the vast majority of ZFPs is unknown and cannot be directly inferred by homology due to the diversity of recognition residues present within individual fingers. Given the large number of unique zinc fingers and assemblies present across eukaryotes, a comprehensive predictive recognition model that could accurately estimate the DNA-binding specificity of any ZFP based on its amino acid sequence would have great utility. Toward this goal, we have used the DNA-binding specificities of 678 two-finger modules from both natural and artificial sources to construct a random forest-based predictive model for ZFP recognition. We find that our recognition model outperforms previously described determinant-based recognition models for ZFPs, and can successfully estimate the specificity of naturally occurring ZFPs with previously defined specificities

Synthesizing evidence of diagnostic accuracy

Clinicians have long relied upon diagnostic tests for ‘evidence’ of the presence or absence of a disease or a condition. Similarly, policy makers must evaluate the value of a particular diagnostic test, compare it to any others, and decide which test should be made available or funded. Methods to synthesize evidence from diagnostic test accuracy studies are now emerging and this text examines the methodological basis to the synthesis of diagnostic test accuracy data and describes the processes involved in the conduct of a diagnostic test accuracy systematic review. Although screening studies share some similarities with diagnostic studies and may report similar statistics, screening is typically applied to uncover very early signs of disease or the risk of disease, whereas diagnostic tests are generally applied to individuals with signs or symptoms of disease. Issues of meta-analysis of screening studies are discussed elsewhere.Sarahlouise Jones, Tim Schultz and Yeetey Enuame

Dissociation of halted T7 RNA polymerase elongation complexes proceeds via a forward-translocation mechanism

A recent model for the mechanism of intrinsic transcription termination involves dissociation of the RNA from forward-translocated (hypertranslocated) states of the complex [Yarnell WS, Roberts JW (1999) Science, 284:611-615]. The current study demonstrates that halted elongation complexes of T7 RNA polymerase in the absence of termination signals can also dissociate via a forward-translocation mechanism. Shortening of the downstream DNA or the introduction of a stretch of mismatched DNA immediately downstream of the halt site reduces a barrier to forward translocation and correspondingly reduces the lifetime of halted complexes. Conversely, introduction of a cross-link downstream of the halt site increases the same barrier and leads to an increase in complex lifetime. Introduction of a mismatch within the bubble reduces a driving force for forward translocation and correspondingly increases the lifetime of the complex, but only for mismatches at the upstream edge of the bubble, as predicted by the model. Mismatching only the two most upstream of the eight bases in the bubble provides a maximal increase in complex stability, suggesting that dissociation occurs primarily from early forward-translocated states. Finally, addition in trans of an oligonucleotide complementary to the nascent RNA just beyond the hybrid complements the loss of driving force derived from placement of a mismatch within the bubble, confirming the expected additivity of effects. Thus, forward translocation is likely a general mechanism for dissociation of elongation complexes, both in the presence and absence of intrinsic termination signals

The benefits or otherwise of managing malaria cases with or without laboratory diagnosis: the experience in a district hospital in Ghana.

BACKGROUND: This study was conducted at the Kintampo Municipal Hospital in Ghana to determine whether there was any benefit (or otherwise) in basing the management of cases of suspected malaria solely on laboratory confirmation (microscopy or by RDT) as compared with presumptive diagnosis. METHOD: Children under five years who reported at the Out-Patient Department of the Hospital with axillary temperature ≥37.5°C or with a 48 hr history of fever were enrolled and had malaria microscopy and RDT performed. The attending clinician was blinded from laboratory results unless a request for these tests had been made earlier. Diagnosis of malaria was based on three main methods: presumptive or microscopy and/or RDT. Cost implication for adopting laboratory diagnosis or not was determined to inform malaria control programmes. RESULTS: In total, 936 children were enrolled in the study. Proportions of malaria diagnosed presumptively, by RDT and microscopy were 73.6% (689/936), 66.0% (618/936) and 43.2% (404/936) respectively. Over 50% (170/318) of the children who were RDT negative and 60% (321/532) who were microscopy negative were treated for malaria when presumptive diagnoses were used. Comparing the methods of diagnoses, the cost of malaria treatment could have been reduced by 24% and 46% in the RDT and microscopy groups respectively; the reduction was greater in the dry season (43% vs. 50%) compared with the wet season (20% vs. 45%) for the RDT and microscopy confirmed cases respectively. DISCUSSION/CONCLUSION: Over-diagnosis of malaria was prevalent in Kintampo during the period of the study. Though the use of RDT for diagnosis of malaria might have improved the quality of care for children, it appeared not to have a cost saving effect on the management of children with suspected malaria. Further research may be needed to confirm this