Isolation and identification of halophilic bacteria producing exopolysaccharides from whey and milk permeate

The aim of this research was to utilization of the salt whey and milk permeate to produce exopolysaccharides (EPSs). Where cheese whey is simultaneously an effluent with nutritional value and a strong organic and saline content. However, it is drained in the sewers, the EPSs are highly heterogeneous polymers produced by different species of bacteria and have recently been attracting considerable attention from biotechnologists because of their potential applications in many fields. Thus, we have isolated some halophilic bacteria that showed the ability to produce EPS from whey and milk permeate. A total of 46 strains of moderately halophilic bacteria were isolated from two types of samples. The first type was dairy samples (Baramily cheese whey and mish cheese), while the second type was water samples (salty lake water and its sediments) of Wadi El-Natrun Valley (lakes Hamra, UmRisha, and Baida), Beheira Governorate, Egypt. From isolated strains there are twelve strains were having the ability to produce exopolysaccharides but only seven strains can produce EPS from whey and milk permeate. The growth conditions i.e. concentrations of NaCl, pH value and different incubation temperature, of isolates were determined. The effect of these conditions on the production of EPS was investigated. The obtained results indicated that the optimum conditions for the production of EPS by these strains were 10 % NaCl, pH 7 and the optimum incubation temperature was 37°C. Three strains showed the highest production of exopolysaccharides. These strains were identified using two methods the first method was biology system and the second one was 16S rRNA sequence analysis method. It could be identified as Alteribacillus bidgolensis and Bacillus licheniformis. Alteribacillus bidgolensis (strain P4B) produced the highest amount of EPS (52 g/L) from whey followed by Bacillus licheniformis (DSM 13) (42 g/L), while the highest amount of EPS produced from milk permeate was (43 g/L) by Alteribacillus bidgolensis (strain P4B) followed by Bacillus licheniformis (DSM 13) (36 g/L)

Food Safety - Some Global Trends

Food safety is defined as the concept that food will not cause harm to the consumer when it is prepared and/or eaten according to its intended use. Most food product recalls and food-related outbreaks are fully considered as food safety failures. Many risk-based food safety standards, e.g., HACCP, BRC, SQF, ISO/FSSC 22000, are designed to prevent such issues from occurring. Any food recall or food-related outbreak may be attributed to the likelihood of a risk assessment, which in some way failed to identify and control the risk. The essence and true nature of food safety hazards are affected by resources of the food facility, e.g., human, work environment, infrastructure, availability and accessibility of food safety information. Thus, food specialists should establish and manage the parameters of the applied food safety systems to achieve the food safety objectives that produce food in compliance with regulatory and statutory requirements. It is important to understand what exactly will make an end product unsafe and ensure that the necessary control measures are in place to prevent it from happening. Understanding the basic food safety concepts can lead to improvement of the current food safety systems and/or standards

Production of Ultrafiltered Skim Milk Retentate Powder 1

Raw skim milk retentate with 20% solids produced by UF was subjected to different heat treatments and pH adjustments prior to spray-drying. The heat treatments were 65 degrees C for 30 min, 75 degrees C for 28 s, and 85 degrees C for 28 s. pH was adjusted to 6.4, 6.7, and 7.0. Retentate powders were analyzed for moisture, protein, lactose, fat, ash, titratable acidity, and pH. Physical property determinations included solubility index, dispersibility, viscosity, scorched particles, poured density, packed density, and water absorption isotherm. No interaction effects of heat treatment and pH adjustment were observed. pH adjustments affected ash content and solution viscosity. Heat treatment affected solubility and poured density. pH adjustments and heat treatment had only minor effects on the measured properties