665 research outputs found
Etude Comparative de l’effet de Souches de Champignons Mycorhiziens Arbusculaires sur la Croissance et la Nutrition Minérale du Sésame Cultivé au Sénégal
La croissance et la nutrition minérale de huit variétés locales de Sésame (Sesamum indicum L.) ont été évaluées en réponse à l’inoculation mycorhizienne arbusculaire. Les essais conduits en serre pendant trois mois, ont montré un meilleur développement et une meilleure nutrition minérale chez les plants inoculés comparé aux témoins non inoculés. Les variétés testées se sont révélées très dépendantes de la mycorhization arbusculaire. Le degré de dépendance est généralement supérieur à 55 % et est fortement corrélé à l’isolat fongique et à la variété testée. Il ressort de cette étude que les biofertilisants à base de champignons mycorhiziens procurent des bénéfices importants à la plante, et peuvent être utilisés pour améliorer la croissance et le rendement du sésame.Mots Clés: Biofertilisation, Mycorhizienne, Sesamum indicumThe growth and mineral nutrition of eight local varieties of Sesame (Sesamum indicum L.) were evaluated in response to arbuscular mycorrhiza inoculation in a greenhouse. There was greater plant growth and better mineral nutrition in inoculated pots compared to non-inoculated controls. The varieties tested showed high dependence on arbuscular mycorrhiza. The degree of dependence was generally greater than 55% and was highly correlated with the fungal isolates and variety tested. It is evident that mycorrhizae fungi-based biofertilisers provide significant benefits to the plant and can be used to improve growth and yield in sesame.Keywords: Biofertilisation, Mycorrhiza, Sesamum indicu
Electron-phonon coupling and its evidence in the photoemission spectra of lead
We present a detailed study on the influence of strong electron-phonon
coupling to the photoemission spectra of lead. Representing the strong-coupling
regime of superconductivity, the spectra of lead show characteristic features
that demonstrate the correspondence of physical properties in the normal and
the superconducting state, as predicted by the Eliashberg theory. These
features appear on an energy scale of a few meV and are accessible for
photoemission only by using modern spectrometers with high resolution in energy
and angle.Comment: 4 pages, 4 figures, accepted for publication in Phys. Rev. Let
Effect of an Outpatient Education Program on Knowledge and Healthy Behavior Change of Patients Who Have Undergone Percutaneous Transluminal Coronary Angioplasty
72 leavesAn outpatient education program for patients who have undergone percutaneous transluminal coronary angioplasty was evaluated to determine if there was an increase in knowledge of a change in healthy behaviors as a
result of the program. The sample consisted of 48 patients; 24 who experienced an outpatient
education program and 24 who did not. Three instruments developed by the investigator were used. Both groups completed a demographics form, a knowledge assessment form and a lifestyle assessment form before the program
began. The knowledge assessment form and lifestyle assessment form were completed again six weeks after the program.
Both groups showed a statistically significant increase in knowledge
(p<.05). The experimental group showed a statistically significant increase in
exercise behavior (p<.05). Because both groups showed a statistically significant increase in knowledge, this change could not be attributed to the program. Only the experimental group showed an increase in healthy behaviors, however. Therefore, the education program was effective in influencing patients to become more responsible for their treatment and care
The high-pressure alpha/beta phase transition in lead sulphide (PbS): X-ray powder diffraction and quantum mechanical calculations
The high-pressure behaviour of PbS was investigated by angular dispersive
X-ray powder diffraction up to pressures of 6.8 GPa. Experiments were
accompanied by first principles calculations at the density functional theory
level. By combining both methods reliable data for the elastic properties of
rock-salt type alpha- and high-pressure beta-PbS could be obtained. beta-PbS
could be determined to crystallise in the CrB-type (B33), with space group
Cmcm. The reversible ferro-elastic alpha/beta transition is of first order. It
is accompanied by a large volume discontinuity of about 5% and a coexistence
region of the two phases. A gliding mechanism of {001} bilayers along one of
the cubic -directions governs the phase transition which can be described
in terms of group/subgroup relationships via a common subgroup, despite its
reconstructive character. The quadrupling of the primitive unit cell indicates
a wave vector (0,0,pi/a) on the Delta-line of the Brillouin zone.Comment: 7 pages, 5 figures, 3 tables submitted to EP
Experimental study of slow sand filtration for the treatment of various wastewaters in tropical environment
Wastewater treatment by slow sand filtration is a biological process which consists in filtering wastewater through a porous media. The objective of this study was to evaluate the performances of this biotechnology under tropical climate. Three sand filters were monitored at ONAS (Cambérène wastewater treatment plant in Dakar, Senegal). The filters were built with local materials, and were constituted with a feeding tank of approximately 180 litters, a column (two meters high and 30-25 cm diameter). The filters were filled with sand (75 cm), gravel (25 cm) and wastewater (90 cm). The results showed that with sand of d10 equal to 0.58 mm and flow rate of 0.05 m/h, the filtration cycle was relatively long for the first run with pretreated wastewater. At the level of the primary settling tank, with flow a rate of 0.08 m/h, the removal rates were about 12.75%, 16.31%, 12.92%, 5.45%, 7.09% and 10.50% for TSS, COD, BOD5, nitrogen phosphorus and faecal coliforms respectively. At the level of the clarifier, with a filtration of 0.15 m/h, the removals were 1.4% for TSS, 1.84% for COD, 1.09% for BOD5 11.38% for nitrogen, 5.18% for phosphorus and 1.74% of faecal coliforms.Keywords: Flow rate; filtration cycle, removal; sand filtration; tropical climate, wastewater
Signature of quantum criticality in photoemission spectroscopy at elevated temperature
A quantum phase transition (QPT) in a heavy-fermion (HF) compound may destroy
the Fermi liquid groundstate. However, the conditions for this breakdown have
remained obscure. We report the first direct investigation of heavy
quasiparticle formation and breakdown in the canonical system
CeCuAu by ultraviolet photoemission spectroscopy at elevated
temperatures without the complications of lattice coherence. Surprisingly, the
single-ion Kondo energy scale exhibits an abrupt step near the quantum
critical Au concentration of . We show theoretically that this step is
expected from a highly non-linear renormalization of the local spin coupling at
each Ce site, induced by spin fluctuations on neighboring sites. It provides a
general high-temperature indicator for HF quasiparticle breakdown at a QPT.Comment: Published version, PRL, minor changes in wordin
Comparing variant calling algorithms for target-exon sequencing in a large sample
Abstract
Background
Sequencing studies of exonic regions aim to identify rare variants contributing to complex traits. With high coverage and large sample size, these studies tend to apply simple variant calling algorithms. However, coverage is often heterogeneous; sites with insufficient coverage may benefit from sophisticated calling algorithms used in low-coverage sequencing studies. We evaluate the potential benefits of different calling strategies by performing a comparative analysis of variant calling methods on exonic data from 202 genes sequenced at 24x in 7,842 individuals. We call variants using individual-based, population-based and linkage disequilibrium (LD)-aware methods with stringent quality control. We measure genotype accuracy by the concordance with on-target GWAS genotypes and between 80 pairs of sequencing replicates. We validate selected singleton variants using capillary sequencing.
Results
Using these calling methods, we detected over 27,500 variants at the targeted exons; >57% were singletons. The singletons identified by individual-based analyses were of the highest quality. However, individual-based analyses generated more missing genotypes (4.72%) than population-based (0.47%) and LD-aware (0.17%) analyses. Moreover, individual-based genotypes were the least concordant with array-based genotypes and replicates. Population-based genotypes were less concordant than genotypes from LD-aware analyses with extended haplotypes. We reanalyzed the same dataset with a second set of callers and showed again that the individual-based caller identified more high-quality singletons than the population-based caller. We also replicated this result in a second dataset of 57 genes sequenced at 127.5x in 3,124 individuals.
Conclusions
We recommend population-based analyses for high quality variant calls with few missing genotypes. With extended haplotypes, LD-aware methods generate the most accurate and complete genotypes. In addition, individual-based analyses should complement the above methods to obtain the most singleton variants.http://deepblue.lib.umich.edu/bitstream/2027.42/110906/1/12859_2015_Article_489.pd
Comparing variant calling algorithms for target-exon sequencing in a large sample
Abstract
Background
Sequencing studies of exonic regions aim to identify rare variants contributing to complex traits. With high coverage and large sample size, these studies tend to apply simple variant calling algorithms. However, coverage is often heterogeneous; sites with insufficient coverage may benefit from sophisticated calling algorithms used in low-coverage sequencing studies. We evaluate the potential benefits of different calling strategies by performing a comparative analysis of variant calling methods on exonic data from 202 genes sequenced at 24x in 7,842 individuals. We call variants using individual-based, population-based and linkage disequilibrium (LD)-aware methods with stringent quality control. We measure genotype accuracy by the concordance with on-target GWAS genotypes and between 80 pairs of sequencing replicates. We validate selected singleton variants using capillary sequencing.
Results
Using these calling methods, we detected over 27,500 variants at the targeted exons; >57% were singletons. The singletons identified by individual-based analyses were of the highest quality. However, individual-based analyses generated more missing genotypes (4.72%) than population-based (0.47%) and LD-aware (0.17%) analyses. Moreover, individual-based genotypes were the least concordant with array-based genotypes and replicates. Population-based genotypes were less concordant than genotypes from LD-aware analyses with extended haplotypes. We reanalyzed the same dataset with a second set of callers and showed again that the individual-based caller identified more high-quality singletons than the population-based caller. We also replicated this result in a second dataset of 57 genes sequenced at 127.5x in 3,124 individuals.
Conclusions
We recommend population-based analyses for high quality variant calls with few missing genotypes. With extended haplotypes, LD-aware methods generate the most accurate and complete genotypes. In addition, individual-based analyses should complement the above methods to obtain the most singleton variants.http://deepblue.lib.umich.edu/bitstream/2027.42/134735/1/12859_2015_Article_489.pd
Demand-Side Food Policies for Public and Planetary Health
Background: The current food system has major consequences for the environment and for human health. Alignment of the food policy areas of mitigating climate change and public health will ensure coherent and effective policy interventions for sustaining human health and the environment. Thispaperexploresliteratureondemand-sidepoliciesthataimtoreduceconsumptionof animal-basedfoods,increaseplant-basedfoods,andreduceoverconsumption. Methods:Wesearched for publications, published between January 2000 and December 2019, considering the above policy domains. Articles were distinguished for type of policy instrument, for topic via keywords and examples were given. Results: The majority of demand-side policies focus on preventing overweight and obesity, using all types of policy instruments including more forceful market-based policies. Hardly any examples of public policies explicitly aiming to lower animal-based foods consumption were found. Policies combining health and sustainability objectives are few and mainly of the information type. Discussion: Moving towards environmentally sustainable and healthy diets is challenging as the implemented demand-side policies focus largely on human health, and not yet on environmental outcomes, or on win-wins. Policies targeting foods from the health perspective can contribute to lower environmental impacts, by indicating suitable animal-based food replacers, and aiming at avoiding overconsumption of energy dense-nutrient poor foods. Preferred policies include a variety of instruments, including strong measures. Conclusions: Working solutions are available to ensure coherent and effective demand side food policies aligning public health and environmental aims. Implementation of aligned and effective policy packages is urgent and needed.© 2020 by the authorspublishedVersio
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