A Dataset of Flash and Ambient Illumination Pairs from the Crowd

Illumination is a critical element of photography and is essential for many computer vision tasks. Flash light is unique in the sense that it is a widely available tool for easily manipulating the scene illumination. We present a dataset of thousands of ambient and flash illumination pairs to enable studying flash photography and other applications that can benefit from having separate illuminations. Different than the typical use of crowdsourcing in generating computer vision datasets, we make use of the crowd to directly take the photographs that make up our dataset. As a result, our dataset covers a wide variety of scenes captured by many casual photographers. We detail the advantages and challenges of our approach to crowdsourcing as well as the computational effort to generate completely separate flash illuminations from the ambient light in an uncontrolled setup. We present a brief examination of illumination decomposition, a challenging and underconstrained problem in flash photography, to demonstrate the use of our dataset in a data-driven approach. Keywords: Flash photography; Dataset collection; Crowdsourcing; Illumination decompositio

Molecular cloning, expression, and functional analysis of the chitin synthase 1 gene and its two alternative splicing variants in the white-backed planthopper, Sogatella furcifera (Hemiptera: Delphacidae)

Abstract Chitin synthase is responsible for chitin synthesis in the cuticles and cuticular linings of other tissues in insects. We cloned two alternative splicing variants of the chitin synthase 1 gene (SfCHS1) from the white-backed planthopper, Sogatella furcifera. The full-length cDNA of the two variants (SfCHS1a and SfCHS1b) consists of 6408 bp, contains a 4719-bp open reading frame encoding 1572 amino acids, and has 5′ and 3′ non-coding regions of 283 and 1406 bp, respectively. The two splicing variants occur at the same position in the cDNA sequence between base pairs 4115 and 4291, and consist of 177 nucleotides that encode 59 amino acids but show 74.6% identity at the amino acid level. Analysis in different developmental stages showed that expression of SfCHS1 and SfCHS1a were highest just after molting, whereas SfCHS1b reached its highest expression level 2 days after molting. Further, SfCHS1 and SfCHS1a were mainly expressed in the integument, whereas SfCHS1b was predominately expressed in the gut and fat body. RNAi-based gene silencing inhibited transcript levels of the corresponding mRNAs in S. furcifera nymphs injected with double-stranded RNA of SfCHS1, SfCHS1a, and SfCHS1b, resulted in malformed phenotypes, and killed most of the treated nymphs. Our results indicate that SfCHS1 may be a potential target gene for RNAi-based S. furcifera control