Evidence for Increased Beta-Adrenoreceptor Responsiveness Induced by 14 Days of Simulated Microgravity in Humans

We studied hemodynamic responses to alpha and beta receptor agonists in 8 healthy men ( 38+- 2 yrs) before and after 14 days of 6 degree head-down tilt (HDT) to test the hypothesis that increased adrenergic responsiveness is induced by prolonged exposure to microgravity. Immediately following a 30-min baseline period, a steady-state infusion of isoproterenol (ISO) was used to assess beta 1- and beta 2-adrenergic responsiveness. ISO was infused at three graded constant rates of 0.005, 0.01 and 0.02 ug/kg/min. After heart rate and blood pressure had been allowed to return to baseline levels following ISO infusion graded infusion of phenylephrine (PE) was used to assess responsiveness of alpha I-vascular receptors. PE was infused at three graded constant rates of 0.25, 0.50 and 1.00 ug/kg/min. Each infusion interval for both drugs was 9 min. During the infusions, constant monitoring of beat-to-beat blood pressure and heart rate was performed and leg blood flow was measured with occlusion plethysmography at each infusion level. The slopes calculated from linear regressions between ISO and PE doses and changes in heart rate, blood pressure, and leg vascular resistance for each subject were used to represent alpha- and beta- adrenoreceptor responsiveness. Fourteen days HDT increased the slopes of heart rate (1056 +- 107 to 1553 +- 83 beats/ug/kg/min; P= 0.014) and vasodilation (-469ft +- 111 to -l446 +- 309 PRU/ug/kg/min; P =0.0224) to ISO infusion. There was no alteration in blood pressure or vascular resistance responses to PE infusion after HDT. Our results provide evidence that microgravity causes selective increases in beta 1- and beta 2-adrenergic responsiveness without affecting alpha 1-vascular responses

Enhanced Biostability and Cellular Uptake of Zinc Oxide Nanocrystals Shielded with Phospholipid Bilayer

The widespread use of ZnO nanomaterials for biomedical applications, including therapeutic drug delivery or stimuli-responsive activation, as well as imaging, imposes a careful control over the colloidal stability and long-term behaviour of ZnO in biological media. Moreover, the effect of ZnO nanostructures on living cells, in particular cancer cells, is still under debate. This paper discusses the role of surface chemistry and charge of zinc oxide nanocrystals, of around 15 nm in size, which influence their behaviour in biological fluids and effect on cancer cells. In particular, we address this problem by modifying the surface of pristine ZnO nanocrystals (NCs), rich of hydroxyl groups, with positively charged amino-propyl chains or, more innovatively, by self-assembling a double-lipidic membrane, shielding the ZnO NCs. Our findings show that the prolonged immersion in simulated human plasma and in the cell culture medium leads to highly colloidally dispersed ZnO NCs only when coated by the lipidic bilayer. In contrast, the pristine and amine-functionalized NCs form huge aggregates after already one hour of immersion. Partial dissolution of these two samples into potentially cytotoxic Zn2+ cations takes place, together with the precipitation of phosphate and carbonate salts on the NCs’ surface. When exposed to living HeLa cancer cells, higher amounts of lipid-shielded ZnO NCs are internalized with respect to the other samples, thus showing a reduced cytotoxicity, based on the same amount of internalized NCs. These results pave the way for the development of novel theranostic platforms based on ZnO NCs. The new formulation of ZnO shielded with a lipid-bilayer will prevent strong aggregation and premature degradation into toxic by-products, and promote a highly efficient cell uptake for further therapeutic or diagnostic functions

GALEX selected Lyman Break Galaxies at z~2: Comparison with other Populations

We present results of a search for bright Lyman break galaxies at 1.5<=z<=2.5 in the GOODS-S field using a NUV-dropout technique in combination with color-selection. We derived a sample of 73 LBG candidates. We compare our selection efficiencies to BM/BX- and BzK methods (techniques solely based on ground-based data sets), and find the NUV data to provide greater efficiency for selecting star-forming galaxies. We estimate LBG candidate ages, masses, star formation rates, and extinction from fitting PEGASE synthesis evolution models. We find about 20% of our LBG candidates are comparable to infrared luminous LBGs or sub-millimeter galaxies which are thought to be precursors of massive elliptical galaxies today. Overall, we can show that although BM/BX and BzK methods do identify star-forming galaxies at z~2, the sample they provide biases against those star-forming galaxies which are more massive and contain sizeable red stellar populations. A true Lyman break criterion at z~2 is therefore more directly comparable to the populations found at z~3, which does contain a red fraction.Comment: 14 pages, 11 figures, accepted for publication in Ap

Insights into the shock initiation/detonation of homogeneous and heterogeneous HE

It has long been known that there are fundamental differences between homogeneous and heterogeneous high explosives. The shock initiation behavior of these materials was first described in the literature by Campbell et al, in 1961. Chaiken was also involved in describing this process for liquid nitromethane. Since then, there have been a number of studies which have added considerable incite into the shock initiation/detonation behavior of these materials. We only give a few references here (Refs. 4 - 11) and these should be considered representative; e.g. they do not represent an exhaustive list of references available. Many of these studies were done on homogeneous explosives, most often nitromethane (NM) and include particle velocity gauge measurements, optical temperature measurements, VISAR measurements, as well as streak camera measurements of interfaces. In some cases NM was heterogenized by gelling and adding silica particles. Homogeneous materials are typically liquids or single crystals in which there are a minimal number of physical imperfections (e.g. bubbles or voids) that can cause perturbations in the input shock and the flow behind it. Homogeneous materials viewed with macroscopic probes characteristic of detonation physics experiments appear uniform. Heterogeneous explosives are generally all other types; these are usually pressed, cast, machined, or extruded into the shapes or parts desired. These materials contain imperfections of a variety of types that cause fluid-mechanical irregularities (called hot spots) when a shock or detonation wave passes over them. Such hot spots cause associated space/time fluctuations in the thermodynamic fields (e.g., the pressure or temperature fields) in the material. These thermodynamic variations affect the local chemical-heat-release rate - they produce an average heat-release rate that is a combination of chemistry and mechanics. Hot spots could be the result of voids, shock interactions, jetting, shock impedance mismatches, etc. Shock initiation of homogeneous explosives is due to a thermal explosion that occurs in the material shocked the longest. This reaction produces a reactive wave that grows behind the front and eventually overtakes the front. The reactive wave may grow into what is called a superdetonation before it overtakes the initial shock and settles down to a steady detonation. The shock initiation process in heterogeneous explosives differs a great deal because the hot spots cause early chemical reaction as soon as the shock passing over a region creates them. This causes reactive growth both in and behind the shock front. This leads to a relatively smooth growth of the initiating shock to a detonation, in contrast to the abrupt changes that occur in the homogeneous case. These differences are apparent in both the in-situ reaction wave profiles and the acceleration of the shock front

Absolute Flux Calibration of the IRAC Instrument on the Spitzer Space Telescope using Hubble Space Telescope Flux Standards

The absolute flux calibration of the James Webb Space Telescope will be based on a set of stars observed by the Hubble and Spitzer Space Telescopes. In order to cross-calibrate the two facilities, several A, G, and white dwarf (WD) stars are observed with both Spitzer and Hubble and are the prototypes for a set of JWST calibration standards. The flux calibration constants for the four Spitzer IRAC bands 1-4 are derived from these stars and are 2.3, 1.9, 2.0, and 0.5% lower than the official cold-mission IRAC calibration of Reach et al. (2005), i.e. in agreement within their estimated errors of ~2%. The causes of these differences lie primarily in the IRAC data reduction and secondarily in the SEDs of our standard stars. The independent IRAC 8 micron band-4 fluxes of Rieke et al. (2008) are about 1.5 +/- 2% higher than those of Reach et al. and are also in agreement with our 8 micron result.Comment: 16 pages, 6 figure

Thoracic and Lumbar Vertebral Bone Mineral Density Changes in a Natural Occurring Dog Model of Diffuse Idiopathic Skeletal Hyperostosis

Ankylosing spinal disorders can be associated with alterations in vertebral bone mineral density (BMD). There is however controversy about vertebral BMD in patients wuse idiopathic skeletal hyperostosis (DISH). DISH in Boxer dogs has been considered a natural occurring disease model for DISH in people. The purpose of this study was to compare vertebral BMD between Boxers with and without DISH. Fifty-nine Boxers with (n=30) or without (n=29) DISH that underwent computed tomography were included. Vertebral BMD was calculated for each thoracic and lumbar vertebra by using an earlier reported and validated protocol. For each vertebral body, a region of interest was drawn on the axial computed tomographic images at three separate locations: immediately inferior to the superior end plate, in the middle of the vertebral body, and superior to the inferior end plate. Values from the three axial slices were averaged to give a mean Hounsfield Unit value for each vertebral body. Univariate statistical analysis was performed to identify factors to be included in a multivariate model. The multivariate model including all dogs demonstrated that vertebral DISH status (Coefficient 24.63; 95% CI 16.07 to 33.19; p <0.001), lumbar vertebrae (Coefficient -17.25; 95% CI -23.42 to -11.09; p < 0.01), and to a lesser extent higher age (Coefficient -0.56; 95% CI -1.07 to -0.05; p = 0.03) were significant predictors for vertebral BMD. When the multivariate model was repeated using only dogs with DISH, vertebral DISH status (Coefficient 20.67; 95% CI, 10.98 to 30.37; p < 0.001) and lumbar anatomical region (Coefficient -38.24; 95% CI, -47.75 to -28.73; p < 0.001) were again predictors for vertebral BMD but age was not. The results of this study indicate that DISH can be associated with decreased vertebral BMD. Further studies are necessary to evaluate the clinical importance and pathophysiology of this finding

Atomic Resonance and Scattering

Contains reports on eight research projects.National Science Foundation (Grant PHY79-09743)National Bureau of Standards (Grant NB-8-NAHA-3017)Joint Services Electronics Program (Contract DAAG29-80-C-0104)National Science Foundation (Grant PHY82-10486)U.S. Navy - Office of Naval Research (Contract N00014-79-C-0183)National Science Foundation (Grant CHE79-02967-A04)U.S. Air Force - Office of Scientific Research (Contract AFOSR-81-0067)Joint Services Electronics Program (Contract DAAG29-83-K-0003

Early postnatal hypotension and developmental delay at 24 months of age among extremely low gestational age newborns

To evaluate, in extremely low gestational age newborns (ELGANs), relationships between indicators of hypotension during the first 24 postnatal hours and developmental delay at 24 months of age

Reproducibility of a peripheral quantitative computed tomography scan protocol to measure the material properties of the second metatarsal

Background: Peripheral quantitative computed tomography (pQCT) is an established technology that allows for the measurement of the material properties of bone. Alterations to bone architecture are associated with an increased risk of fracture. Further pQCT research is necessary to identify regions of interest that are prone to fracture risk in people with chronic diseases. The second metatarsal is a common site for the development of insufficiency fractures, and as such the aim of this study was to assess the reproducibility of a novel scanning protocol of the second metatarsal using pQCT. Methods. Eleven embalmed cadaveric leg specimens were scanned six times; three times with and without repositioning. Each foot was positioned on a custom-designed acrylic foot plate to permit unimpeded scans of the region of interest. Sixty-six scans were obtained at 15% (distal) and 50% (mid shaft) of the second metatarsal. Voxel size and scan speed were reduced to 0.40 mm and 25 mm.sec-1. The reference line was positioned at the most distal portion of the 2nd metatarsal. Repeated measurements of six key variables related to bone properties were subject to reproducibility testing. Data were log transformed and reproducibility of scans were assessed using intraclass correlation coefficients (ICC) and coefficients of variation (CV%). Results: Reproducibility of the measurements without repositioning were estimated as: trabecular area (ICC 0.95; CV% 2.4), trabecular density (ICC 0.98; CV% 3.0), Strength Strain Index (SSI) - distal (ICC 0.99; CV% 5.6), cortical area (ICC 1.0; CV% 1.5), cortical density (ICC 0.99; CV% 0.1), SSI - mid shaft (ICC 1.0; CV% 2.4). Reproducibility of the measurements after repositioning were estimated as: trabecular area (ICC 0.96; CV% 2.4), trabecular density (ICC 0.98; CV% 2.8), SSI - distal (ICC 1.0; CV% 3.5), cortical area (ICC 0.99; CV%2.4), cortical density (ICC 0.98; CV% 0.8), SSI - mid shaft (ICC 0.99; CV% 3.2). Conclusions: The scanning protocol generated excellent reproducibility for key bone properties measured at the distal and mid-shaft regions of the 2 nd metatarsal. This protocol extends the capabilities of pQCT to evaluate bone quality in people who may be at an increased risk of metatarsal insufficiency fractures

Radiation exposure in X-ray-based imaging techniques used in osteoporosis

Recent advances in medical X-ray imaging have enabled the development of new techniques capable of assessing not only bone quantity but also structure. This article provides (a) a brief review of the current X-ray methods used for quantitative assessment of the skeleton, (b) data on the levels of radiation exposure associated with these methods and (c) information about radiation safety issues. Radiation doses associated with dual-energy X-ray absorptiometry are very low. However, as with any X-ray imaging technique, each particular examination must always be clinically justified. When an examination is justified, the emphasis must be on dose optimisation of imaging protocols. Dose optimisation is more important for paediatric examinations because children are more vulnerable to radiation than adults. Methods based on multi-detector CT (MDCT) are associated with higher radiation doses. New 3D volumetric hip and spine quantitative computed tomography (QCT) techniques and high-resolution MDCT for evaluation of bone structure deliver doses to patients from 1 to 3 mSv. Low-dose protocols are needed to reduce radiation exposure from these methods and minimise associated health risks