The role of C-terminal amidation in the membrane interactions of the anionic antimicrobial peptide, maximin H5

Maximin H5 is an anionic antimicrobial peptide from amphibians, which carries a C-terminal amide moiety, and was found to be moderately haemolytic (20%). The α-helicity of the peptide was 42% in the presence of lipid mimics of erythrocyte membranes and was found able to penetrate (10.8mNm(-1)) and lyse these model membranes (64 %). In contrast, the deaminated peptide exhibited lower levels of haemolysis (12%) and α-helicity (16%) along with a reduced ability to penetrate (7.8mNm(-1)) and lyse (55%) lipid mimics of erythrocyte membranes. Taken with molecular dynamic simulations and theoretical analysis, these data suggest that native maximin H5 primarily exerts its haemolytic action via the formation of an oblique orientated α-helical structure and tilted membrane insertion. However, the C-terminal deamination of maximin H5 induces a loss of tilted α-helical structure, which abolishes the ability of the peptide's N-terminal and C-terminal regions to H-bond and leads to a loss in haemolytic ability. Taken in combination, these observations strongly suggest that the C-terminal amide moiety carried by maximin H5 is required to stabilise the adoption of membrane interactive tilted structure by the peptide. Consistent with previous reports, these data show that the efficacy of interaction and specificity of maximin H5 for membranes can be attenuated by sequence modification and may assist in the development of variants of the peptide with the potential to serve as anti-infective

Frames: A Corpus for Adding Memory to Goal-Oriented Dialogue Systems

This paper presents the Frames dataset (Frames is available at http://datasets.maluuba.com/Frames), a corpus of 1369 human-human dialogues with an average of 15 turns per dialogue. We developed this dataset to study the role of memory in goal-oriented dialogue systems. Based on Frames, we introduce a task called frame tracking, which extends state tracking to a setting where several states are tracked simultaneously. We propose a baseline model for this task. We show that Frames can also be used to study memory in dialogue management and information presentation through natural language generation

Knowledge on Sexually Transmitted Infections among school-going adolescents in the Sunyani West District of Ghana

The physiological and behavioural characteristics associated with adolescence increase the risk of contracting HIV and other sexually transmitted infections which may have devastating effects on their sexual and reproductive health. The aim of this study was to assess the knowledge of sexually transmitted infections of school going adolescents in the Sunyani West District of Ghana. A cross-sectional study design was used in gathering appropriate survey data from 316 senior high school students. Descriptive and univariate analyses were conducted using STATA version 14.0 software. The age group 16 - 18 years were the majority of respondents. More male than female adolescents knew about all four areas of knowledge as regards sexually transmitted infections. The study showed a statistically significant association (P = 0.001) between level of knowledge of sexually transmitted infections among adolescents and their class/form. Adolescents had good knowledge about sexually transmitted infections despite some misconceptions that could affect their sexual behaviour. Educational levels of students have to be considered in the design of sex education curriculum

The Application of Building Modifications and their Effects on Energy Consumption in Buildings

A huge amount of energy is used for air-conditioning in residential buildings in hot climates. Passive design features such as shading and advanced glazing can help to reduce energy use and carbon emissions, and thus mitigate the impact on climate change. This paper aimed at demonstrating how the application of selected modification devices such as solar films and shading devices affects the energy consumption patterns and levels in a residential building. A model of a building was constructed with VE using “Model IT” module, which was then analysed in a variety of different ways. A Virtual Integrated Environmental Solutions (IES-VE) was used to assess the energy gain and consumption parameters such as solar gains, shading devices, solar cloud and chilli clouds in residential buildings in Tripoli, Libya. The findings indicate that the best way to control and reduce the energy gains pattern in a building is to introduce energy modification devices such as shading device, solar films, emissivity paints and roof slab absorbers among others. In specific terms, the best device would be the application of external solar film, follow by shading device and internal solar film. An application of emissivity paints and roof slab absorbers does not contribute significantly to the energy reduction in the building. The study concludes that the application of modification devices in buildings can reduces the heat gain significantly. This study underscores the need and importance of the applications of energy modification devices in buildings in order to reduce their energy gains in the context of tropical regions. Though the climatological characteristics of tropical regions are similar, the generalisation of the findings in this study requires caution since the findings are limited in geographical context. Future research should also explore the impact of urban forms, street layout and orientation on solar penetration and energy use in buildings. Keywords: Architecture, Buildings, Climate change, Energy consumption, Energy gains, Liby

Readability and content analysis of lifestyle education resources for weight management in Australian general practice

© 2016 El-Haddad et al. Background: Weight management education is one of the key strategies to assist patients to manage their weight. Educational resources provide an important adjunct in the chain of communication between practitioners and patients. However, one in five Australian adults has low health literacy. The purpose of this study was to assess the readability and analyse the content of weight management resources. Methods: This study is based on the analysis of 23 resources found in the waiting rooms of ten Sydney-based general practices and downloaded from two clinical software packages used at these practices. The reading grade level of these resources was calculated using the Flesch Reading Ease, Flesch-Kincaid Grade Level, Fry Readability Graph, and the Simplified Measure of Gobbledygook. Resources' content was analysed for the presence of dietary, physical activity, and behaviour change elements, as recommended by the Clinical practice guidelines for the management of overweight and obesity in adults, adolescents, and children in Australia. Results: The resources' average reading grade level was for a 10th grader (9.5 ± 1.8). These findings highlight that the average reading grade level was two grades higher than the recommended reading grade level for health education resources of 8th grade level or below. Seventy percent of resources contained dietary and behaviour change elements. Physical activity was included in half of the resources. Two messages were identified to be inconsistent with the guidelines and three messages had no scientific basis. Conclusion: A body of evidence now exists that supports the need to develop evidence-based education resources for weight management that place low demand on literacy, without compromising content accuracy. The findings from this study suggest that there is significant room for improvement in the educational resources provided in general practices

The relationship of female physical attractiveness to body fatness

Funding This work was supported by NSFC grant 91431102 from the National Science Foundation of China. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Acknowledgements We are grateful to all the participants from all the countries and all the members of Molecular Energetics Group for their help on the investigation and discussion of the results.Peer reviewedPublisher PD

Parton rescattering and screening in Au+Au collisions at RHIC

We study the microscopic dynamics of quarks and gluons in relativistic heavy ion collisions in the framework of the Parton Cascade Model. We use lowest order perturbative QCD cross sections with fixed lower momentum cutoff p_0. We calculate the time-evolution of the Debye-screening mass for Au+Au collisions at sqrt(s)=200 GeV per nucleon pair. The screening mass is used to determine a lower limit for the allowed range of p_0. We also determine the energy density reached through hard and semi-hard processes at RHIC, obtain a lower bound for the rapidity density of charged hadrons produced by semihard interactions, and analyze the extent of perturbative rescattering among partons.Comment: 6 pages, 4 figures, uses RevTeX 4.0; revised version with minor corrections and one updated figur