A highly sensitive sandwich ELISA for the determination of glycomacropeptide to detect liquid whey in raw milk

Milk processing industries and distributors have problems with adulteration of liquid milk by the addition of bovine cheese whey. Recently, the detection of fraudulent manipulation of milk with whey has focused on the identification of glycomacropeptide (GMP). Current non-immunological methods to detect GMP in dairy products are expensive and time-consuming or have low sensitivity. In this study, a novel sandwich enzyme-linked immunosorbent assay (ELISA) for the detection and quantification of whey in raw milk was developed, using a polyclonal rabbit anti-GMP antibody. Calibration curves were constructed by analyzing raw milk standards containing different known concentrations of liquid cheese whey (0.02–20%). The method had a detection limit of 0.047% (v/v) and a quantification limit of 0.14% (v/v). The antibody showed high specificity and no cross-reaction with milk components (other than κ-casein) and was successful in detecting GMP in dairy commercial products. The recovery ratio was between 95.62% and 113.88% for all matrices tested. The intra-assay and interassay coefficients of variation were <6% and <7%, respectively. Finally, it can be stored for 3 months in the form of a ready-to-use kit, while maintaining its accuracy and reproducibility

El quiste óseo aneurismático en niños y adolescentes

Desde el año 1981 hasta el año 1996 hemos tratado en nuestro servicio un total de 12 pacientes con diagnóstico de Quiste Óseo Aneurismático (QOA). Esta entidad clínico-patológica continúa hoy teniendo un origen incierto que en la mayoría de las ocasiones no puede reconocerse. Los tratamientos empleados fueron: legrado e injerto óseo en siete casos, resección en bloque en tres casos, y en 2 casos tratamiento expectante. Los resultados del tratamiento quirúrgico fueron satisfactorios en el 75% de los casos, encontrando un 25% de recidivas, que ocurrieron en los pacientes más jóvenes con quistes más agresivos. El tiempo medio de seguimiento fue de 4 años (mínimo 1 - máximo 8). Realizamos en el trabajo, además, una comparación con otros métodos de tratamiento publicados como son la crioterapia intratumoral y la embolizacón arterial selectiva, los cuales encontramos de utilidad relativa ya que requieren una alta especialización y una selección precisa de los casos. También la radioterapia que tiene una utilidad restringida a aquellos casos de inaccesibilidad operatoria.From 1981 to 1996 twelve patients were treated in our orthopaedic department with the diagnosis of Aneurismal Bone Cyst (ABC). This pathologic and clinical entity has an unknown etiology that in the majority of cases it can¿t be elucidated. The threatment was: curettage and bone graft in seven cases; in bloc excision in three cases and expectant in two. Surgical treatment results were satisfactory in 75% of cases, with 25% of local recurrences that took place in younger patients with more aggressive cysts. Mean follow-up was four years (range: 1-8). In this study we make a comparison with other methods of treatment reported like intratumoral criotherapy and selective arterial embolization. We find these methods of relative utility due to their high level of orthopaedist specialization and precise selection of cases. The radiotherapy has restricted application to cases of unresectable tumours

Fracturas femorales en pacientes portadores de artroplastia de cadera

Se han revisado 25 pacientes con fractura femoral portadores de artroplatia de cadera. Las fracturas se clasifican según los tipos de Johansson. Se encontraron 9 tipo I, 9 tipo II y 7 tipo III. El tratamiento fue en 4 ocasiones conservador y en 21 quirúrgico: 2 osteosíntesis con tornillos de compresión, 9 recambios de vástago y 9 osteosíntesis con placa. Se valora el resultado del tratamiento de la fractura. Consolidaron todas salvo una tras el tratamiento inicial. El resultado de la artroplastia fue valorado según la Escala de Merle D'Aubigne siendo bueno en un 68% de los casos. Las complicaciones fueron 2 consolidaciones en varo, una infección superficial y una infección profunda. Se evalúan los diferentes tratamientos realizados y se expone nuestra actitud actual ante estas fracturas.The present study includes 25 patients with hip replacement who presented an ipsilateral fracture of the femur. According to Johansson's grading system, there were 9 types I fractures, 9 types II and 7 types III. Surgical treatment was indicated in 21 cases, and conservative treatment in 4. Of the 21 patients operated on 9, required stem replacement, 9 internal fixations with plate and screws, and inter-fragmentary compression screws in 2 cases. Outcome assessment showed that, except one case, all fractures healded after treatment. The results of the arthroplasty were good in 68% of cases according to the Merle D'Aubigne scale. Complications included 2 various deformities, one superficial infection and one deep infection

Tratamiento de las fracturas complejas de fémur mediante enclavado intramedular encerrojado de Brooker-Wills

Se realiza un estudio retrospectivo de 19 pacientes con fracturas complejas de fé- mur tratados mediante el clavo bloqueado de Brooker-Wills. La causa más frecuente de dichas fracturas fueron los accidentes de tráfico. Hubo lesiones asociadas en el 63% de los casos. El tiempo medio de seguimiento fue de 27 meses. Nuestros resultados han sido evaluados mediante los criteros de Thorensen, obteniendo un 84% de resultados favorables. Hubo problemas técnicos intraoperatorios en 8 pacientes, la mayoría debidos a la penetración de la cortical femoral distal por una de las aletas. Todas las fracturas consolidaron. La mayor ventaja de este clavo la encontramos en la sencillez del bloqueo distal, lo cual supone una menor exposición a la radiación y un acortamiento del tiempo quirúrgico.A retrospective study of 19 patients with complex fermoral fractures treated with the Brooker-Wills interlocking intramedullary nail is reported. The traffic-accident was the most common cause of these fractures. Associated injuries were found in 63% of cases. The mean time follow-up period was 27 months. Results were assessed according to Thorensen's criteria. We found excellent or good results in 84% of these patients. There were intraoperative technical problems without clinical complications in 8 patients, most of them due to distal femoral cortex penetration with the pins. Union occurred in all fractures. The greatest advantage of this nail comes from the easy insertion of distal pins, providings less exposition to radiation and a shorter operative tim

Effects of cytomegalovirus infection in human neural precursor cells depend on their differentiation state

© 2015, Journal of NeuroVirology, Inc. Cytomegalovirus (CMV) is the most common cause of congenital infection in developed countries and a major cause of neurological disability in children. Although CMV can affect multiple organs, the most important sequelae of intrauterine infection are related to lesions of the central nervous system. However, little is known about the pathogenesis and the cellular events responsible for neuronal damage in infants with congenital infection. Some studies have demonstrated that neural precursor cells (NPCs) show the greatest susceptibility to CMV infection in the developing brain. We sought to establish an in vitro model of CMV infection of the developing brain in order to analyze the cellular events associated with invasion by this virus. To this end, we employed two cell lines as a permanent source of NPC, avoiding the continuous use of human fetal tissue, the human SK-N-MC neuroblastoma cell line, and an immortalized cell line of human fetal neural origin, hNS-1. We also investigated the effect of the differentiation stage in relation to the susceptibility of these cell lines by comparing the neuroblastoma cell line with the multipotent cell line hNS-1. We found that the effects of the virus were more severe in the neuroblastoma cell line. Additionally, we induced hNS-1 to differentiate and evaluated the effect of CMV in these differentiated cells. Like SK-N-MC cells, hNS-1-differentiated cells were also susceptible to infection. Viability of differentiated hNS-1 cells decreased after CMV infection in contrast to undifferentiated cells. In addition, differentiated hNS-1 cells showed an extensive cytopathic effect whereas the effect was scarce in undifferentiated cells. We describe some of the effects of CMV in neural stem cells, and our observations suggest that the degree of differentiation is important in the acquisition of susceptibility.CONACYT (CB16782 and #120452), PROMEP (103.5/10/7697), and FAI-UASLP (C12-FAI-03-62.62).Peer Reviewe

Towards a "Book Publishers Citation Reports". First approach using the "Book Citation Index"

The absence of books and book chapters in the Web of Science Citation Indexes (SCI, SSCI and A&HCI) has always been considered an important flaw but the Thomson Reuters ‘Book Citation Index’ database was finally available in October of 2010 indexing 29,618 books and 379,082 book chapters. The Book Citation Index opens a new window of opportunities for analyzing Humanities and Social Sciences from a bibliometric point of view. The main objective of this article is to analyze different impact indicators referred to the scientific publishers included in the Book Citation Index for the Social Sciences and Humanities fields during 2006-2011. This way we construct what we have called the ‘Book Publishers Citation Reports’. For this, we present a total of 19 rankings according to the different disciplines in Humanities & Arts and Social Sciences & Law with six indicators for scientific publishers.La ausencia de libros y capítulos de libros en los índices de citas presentes en las bases de datos de la Web of Science ha sido tradicionalmente una de sus más importantes debilidades. Sin embargo, Thomson Reuters en Octubre de 2010 lanzó el Book Citation Index, un nuevo índice de citas que contaba con 29.618 libros y 379.082 capítulos de libros. Este producto ha abierto nuevas posibilidades para el análisis bibliométrico de campos como las Humanidades y las Ciencias Sociales. Precisamente el objetivo principal de esta nota es analizar a través de diferentes indicadores las editoriales de los ámbitos de Humanidades y Ciencias Sociales indexadas en el Book Citation Index durante los años 2006-2011. Más concretamente se ha probado la posibilidad de desarrollar un ranking de editoriales de libros basado en la citación y la producción de las mismas. Para ello se presentan una colección de rankings con seis indicadores bibliométricos para un total de 19 disciplinas científicas

Consultoría integral en una empresa de servicios de ingeniería estructural y supervisión e inspección de estructura en el municipio de Guadalajara

Este reporte de consultoría integral en una empresa de servicios de ingeniería estructural y supervisión e inspección de estructura en el municipio de Guadalajara ha sido elaborado por Ricardo Enrique Salinas Quintero, Luis Daniel Muñoz Guerrero, Álvaro Rizzo Martínez, Javier Jiménez Ruiz, Miguel Moreno Morrill, y Karla Nicole Welcomme Delgado, con la dirección de las profesoras Laura Tiburcio Silver y María Elena Ríos Morfín. El documento se estructura por la descripción del proyecto, los aprendizajes, las conclusiones, fuentes consultadas, y lista de anexos. Al efecto, se trabajó con la metodología de la consultoría en MYPE del Centro Universidad Empresa. Las fuentes primarias utilizadas fueron los archivos proporcionados por la empresa, así como las entrevistas y las reuniones con el empresario y los colaboradores. Así mismo también se utilizaron fuentes secundarias como páginas de internet y plataformas de instituciones que ayudaron a complementar la información. Los contenidos principales están integrados por las generalidades y contexto de la empresa, el diagnóstico, el planteamiento estratégico, la propuesta de mejora incluyendo el plan de acción, la implementación, los resultados obtenidos y las recomendaciones finales. Se concluye que las MYPE son empresas que están cruzando una etapa importante en su formación cuando empiezan a caminar por si solas y buscar cursar la etapa de institucionalización que toda organización desea alcanzar. Son empresas que están formadas por trabajadores amateur y empresarios que pusieron todo su tiempo, patrimonio y esfuerzo en crear un nuevo ser que influya en la sociedad y en la vida de todos sus colaboradores.ITESO, A.C

5-Hydroxymethylfurfural (HMF) formation during subcritical water extraction

The aim of this study was to investigate the effect of material type (artichoke leave, lemon peel, flaxseed meal), extraction temperature (50, 100, 120, 140, 160, 180, 200 C) and static extraction time (5, 15, 30, 45 min) on 5-hydroxymethylfurfural (5-HMF) formation during subcritical water extraction. 5-HMF content of artichoke leave and lemon peel extracts increased 7.2 and 26.1 times with the rise of extraction temperature from 160 to 180 C for 5 min during subcritical water extraction, respectively. Besides, 5-HMF content of artichoke leave, lemon peel and flaxseed meal extracts increased 1.4, 2.0 and 4.5 times as static extraction time increased from 15 to 45 min at 180 C during subcritical water extraction, respectively. The highest 5-HMF content of artichoke leave and lemon peel extracts were obtained as 58.83 and 231.21 mg/L at 180 C and 45 min, respectively. However, for flaxseed meal, the highest 5-HMF content (222.94 mg/L) was obtained at 200 C and 15 min during subcritical water extraction.Project Nos. 2014.M80.02.03, 2014.M80.02.04 by Artvin Coruh University Scientific Research Project Uni

Above- and below-ground net primary productivity across ten Amazonian forests on contrasting soils

Copyright © 2009 European Geosciences Union. This is the published version available at http://www.biogeosciences.net/6/2759/2009/bg-6-2759-2009.html © Author(s) 2009. This work is distributed under the Creative Commons Attribution 3.0 License.The net primary productivity (NPP) of tropical forests is one of the most important and least quantified components of the global carbon cycle. Most relevant studies have focused particularly on the quantification of the above-ground coarse wood productivity, and little is known about the carbon fluxes involved in other elements of the NPP, the partitioning of total NPP between its above- and below-ground components and the main environmental drivers of these patterns. In this study we quantify the above- and below-ground NPP of ten Amazonian forests to address two questions: (1) How do Amazonian forests allocate productivity among its above- and below-ground components? (2) How do soil and leaf nutrient status and soil texture affect the productivity of Amazonian forests? Using a standardized methodology to measure the major elements of productivity, we show that NPP varies between 9.3±1.3MgC ha−1 yr−1 (mean±standard error), at a white sand plot, and 17.0±1.4MgC ha−1 yr−1 at a very fertile Terra Preta site, with an overall average of 12.8±0.9MgC ha−1 yr−1. The studied forests allocate on average 64±3% and 36±3% of the total NPP to the above and below-ground components, respectively. The ratio of above-ground and below-ground NPP is almost invariant with total NPP. Litterfall and fine root production both increase with total NPP, while stem production shows no overall trend. Total NPP tends to increase with soil phosphorus and leaf nitrogen status. However, allocation of NPP to below-ground shows no relationship to soil fertility, but appears to decrease with the increase of soil clay content