Automatically tracking feeding behavior in populations of foraging worms

C. elegans feeds on bacteria and other small microorganisms which it ingests using its pharynx, a neuromuscular pump. Currently, measuring feeding behavior requires tracking a single animal, indirectly estimating food intake from population-level metrics, or using restrained animals. Therefore, to enable large throughput feeding measurements of unrestrained, crawling worms on agarose plates, we developed an imaging protocol and a complementary image analysis tool called PharaGlow. We image up to 50 freely moving worms simultaneously and extract locomotion and feeding behaviors. Our tool reliably detects pharyngeal pumping in adult worms with a maximum deviation of 5% in the number of pumps compared to an expert annotator. We demonstrate the tool's robustness and highthroughput capabilities by measuring feeding in different use-case scenarios. This includes tracing pharyngeal dynamics during development, revealing their highly conserved nature throughout all life cycle stages. We also observed pumping after food deprivation, corroborating previous studies in which starvation time strongly influences pumping. Finally, we further validated our behavioral tracker by exploring two previously characterized pumping defective mutants: unc-31 and eat-18. Remarkably, our analysis of eat-18 mutants identified unreported defects in pumping and overall locomotion regulation, highlighting the potential of this toolkit. Pharaglow therefore enables the observation and analysis of the temporal dynamics of food intake with high-throughput and precision in a user-friendly system

Automatically tracking feeding behavior in populations of foraging C. elegans

Caenorhabditis elegans feeds on bacteria and other small microorganisms which it ingests using its pharynx, a neuromuscular pump. Currently, measuring feeding behavior requires tracking a single animal, indirectly estimating food intake from population-level metrics, or using restrained animals. To enable large throughput feeding measurements of unrestrained, crawling worms on agarose plates at a single worm resolution, we developed an imaging protocol and a complementary image analysis tool called PharaGlow. We image up to 50 unrestrained crawling worms simultaneously and extract locomotion and feeding behaviors. We demonstrate the tool’s robustness and high-throughput capabilities by measuring feeding in different use-case scenarios, such as through development, with genetic and chemical perturbations that result in faster and slower pumping, and in the presence or absence of food. Finally, we demonstrate that our tool is capable of long-term imaging by showing behavioral dynamics of mating animals and worms with different genetic backgrounds. The low-resolution fluorescence microscopes required are readily available in C. elegans laboratories, and in combination with our python-based analysis workflow makes this methodology easily accessible. PharaGlow therefore enables the observation and analysis of the temporal dynamics of feeding and locomotory behaviors with high-throughput and precision in a user-friendly system.eLife digest: A small worm called C. elegans is constantly hungry. It spends all its time looking for food or eating. Hunger and environmental factors, like light, influence its feeding behavior. Studying these worms has helped scientists learn how feeding affects health, longevity, and aging. Feeding studies might also help scientists learn how the nervous system works and how it controls feeding. Most studies have used one of two approaches. Scientists may measure how much food a group of C. elegans eat by measuring food before and after it is offered to the worms. Or they restrain individual worms and measure the movement of a tube-like muscle, called the pharynx, which the animals use to vacuum up food. Restraining the worms can alter their behavior or brain activity, and studying group feeding habits may miss individual differences, so neither is optimal. Ideally, scientists could measure the feeding activity of many free-ranging worms, but because the movements of the pharynx are small, that too can be a challenge. Bonnard, Liu et al. developed a software tool that automatically detects and measures feeding behavior in a group of about 30 free-ranging C. elegans simultaneously. In the experiments, Bonnard, Liu et al. genetically engineered worms expressing a fluorescent protein in their pharynx, making it possible to measure its movements with a microscope. They used the microscope to capture images of 30-50 animals at a time as they foraged for food in a dish. Then, they used the software to analyze the data they collected. Over three days and five imaging sessions, Bonnard and Liu et al. tracked the feeding behavior of about 1,000 animals under different conditions. The experiments show that the pharynx grows rapidly during early worm development when the worms quadruple their length, but the rate of pharynx muscle contractions stays the same. They also showed the technique could measure feeding behaviors in animals with different genetic backgrounds, ages, or those engaged in behaviors like mating. The tool allows for larger and longer-term studies of worm feeding behaviors than previous approaches. Bonnard, Liu et al. made their software, called PharaGlow, available for use by other researchers. The tool may make feeding measurements a routine part of C. elegans studies. It will allow scientists to gain new insights into the role of feeding in a range of processes, including aging, fitness, mating, and overall health. Follow-up studies could determine if these findings are general strategies that also apply to other animals

Power Parameters Automated Calculation for Transmission with Intermediate Rolling Bodies and Free Cage

In the article, techniques complex of the transmission with IRBFC power parameters determination are considered. Moreover, software of automated calculation of the power parameters is created based on the techniques. The software consists of two parts: program of the mechanical transmission creating as a computer object; program of the graphical user interface creating. In general, the software makes it possible to determine the distribution of forces and contact stresses in engagement along the cycloidal profile of gear, to determine the maximum force and contact stress in engagement and to generate a file in excel format with all calculated data

The concordance of MRI and arthroscopy in traumatic meniscal lesions in children

SummaryIntroductionTraumatic meniscal lesions in children must be diagnosed quickly and efficiently as a priority in order to conserve the meniscus and safeguard the future of the knee. They are often isolated and difficult to identify clinically. In the diagnostic work up stage, an excessive resort to diagnostic arthroscopy has given way to increasing use of MRI by radiologists without pediatric specialization. The present study examined the agreement between MRI aspect and arthroscopic exploration in traumatic meniscal lesions in children.Patients and methodsSixty-nine knees in children aged 9 to 16 years having undergone MRI followed by arthroscopy for knee trauma between 1995 and 2008 were included in a retrospective design. Discoid meniscus was excluded. Files were reviewed by a single clinician and MRI scans by a radiologist specialized in pediatric pathology. Cases of epiphyseal fusion were excluded. All files were analyzable. Agreement with arthroscopic findings as reference was assessed for presence, location and type of meniscal lesion.ResultsOverall agreement with arthroscopy was respectively 78% and 82% on first and second MRI readings: 77% and 80% for the medial, and 78% and 84% for the lateral meniscus. On the first reading, there were 13 false positives for the medial and 5 for the lateral meniscus, versus 9 and 0 respectively on second reading. Overall sensitivity was 70% on first reading and 64% on second, and overall specificity 81% and 90%, respectively.DiscussionThe present results, in line with the literature, may appear encouraging, but hide considerable disparity between analysis of the medial and of the lateral meniscus: MRI overestimated medial and underestimated lateral meniscus lesions.ConclusionMRI serves only as a support and does not provide sure diagnosis of meniscus lesion. Interpretation should take account of the clinical examination and the pediatric orthopedic specialist's experience.Level of evidenceLevel IV, retrospective study

Translated from Itogi Nauki i Tekhniki, Seriya Sovremennaya Matematika i Ee Prilozheniya

Preliminaries Consider the local SR-geometry (U, D, g), where U is a neighborhood of 0 ∈ R 3 , D is a Martinet-type distribution, which can be taken in the normal form D = Ker ω, ω = dz − y 2 2 dx, and g is a C ω metric on D, which can be written (see Expanding F 1 and F 2 in Taylor series according to the previous weights and identifying at the order p two elements whose Taylor series are the same at the order p, we obtain the following normal forms of order −1 and 0: • Normal form of order −1: (flat case); • Normal form of order 0: 2 dx 2 + (1 + βx + γy) 2 dy 2 , α, β, γ ∈ R. 1.1. Geodesics equations. The energy minimization problem equivalent to the SR-problem is the following optimal control problem: from Pontryagin's maximum principle [9], minimizing solutions are solutions of the following equations: where H ν is the pseudo-Hamiltonian where ν is a constant normalized to 0 or 1/2. A solution of the previous equations is called an extremal; when ν = 1/2 (resp. ν = 0), the solutions are called normal (resp. abnormal), and their projections onto the state space are called the geodesics. They can be easily computed

Progresses in the development of a Step-NC compliant additive manufacturing system

The new standard of numerical control, known as STEP-NC, is categorized as the future of the advanced manufacturing systems. Greater flexibility and interoperability are some potential benefits offered by STEP-NC to meet the challenges of the new industrial landscape that is envisaged with the advent of Industry 4.0. Meanwhile, STEP-NC object-oriented programming has been partially applied and developed for machining processes (milling, turning...). But with the processes of additive manufacturing has not happened the same and the development is still incipient. This work presents the advances in the development of a new STEP-NC compliant additive manufacturing system, focusing particularly on the development of the information model. The application model activities in the IDEF0 nomenclature and application reference model in EXPRESS are presented. The AM-layer-feature concept has been introduced to define the manufacturing feature of additive processes based on material deposition layer-by-layer. Finally, a STEP-NC program generated from the EXPRESS model is presented, which can be implemented on an additive manufacturing system to validate the proposed model.El nuevo estándar de control numérico, conocido como STEP-NC, es categorizado como el futuro de los sistemas avanzados de manufactura. Mayor flexibilidad e interoperabilidad son algunos de los potenciales beneficios que ofrece STEP-NC para enfrentar los desafíos del nuevo panorama industrial que se vislumbra con el advenimiento de la Industria 4.0. Mientras tanto, la programación orientada a objeto de STEP-NC ha sido parcialmente aplicada y desarrollada hasta el momento para los procesos de mecanizado (fresado, torneado…). Pero con los procesos de manufactura aditiva no ha sucedido lo mismo y el desarrollo es aún incipiente. Este trabajo presenta los avances en el desarrollo de un nuevo sistema de manufactura aditiva basado en STEP-NC, enfocándose particularmente sobre el desarrollo del modelo de información. EL modelo de actividades de aplicación en la nomenclatura del IDEF0 y el modelo de referencia de aplicación en EXPRESS son presentados. El concepto AM-layer-feature ha sido introducido para definir la feature de manufactura de los procesos aditivos basados en deposición de material capa por capa. Finalmente, se presenta un programa en STEP-NC generado desde el modelo EXPRESS, el cual puede ser implementado sobre un sistema de manufactura aditiva para validar el modelo propuesto

Influence of metal process micronic and submicronic particles on vegetables quality and ecosystems

International audienceImpact of atmospheric process particles enriched with metals (PM) on various vegetables was studied. Foliar metal interception was measured and calculated. Soil-plant transfer and phyto-toxicity were also studied. Influence of species and washing procedure on metal burning was observed. High correlation was obtained between measured and simulated lead plant uptake values. Ageing effect in polluted soils was highlighted with stabilisation or mobilization of metals in function of contact duration between soils and PM

Geometric Approach to Pontryagin's Maximum Principle

Since the second half of the 20th century, Pontryagin's Maximum Principle has been widely discussed and used as a method to solve optimal control problems in medicine, robotics, finance, engineering, astronomy. Here, we focus on the proof and on the understanding of this Principle, using as much geometric ideas and geometric tools as possible. This approach provides a better and clearer understanding of the Principle and, in particular, of the role of the abnormal extremals. These extremals are interesting because they do not depend on the cost function, but only on the control system. Moreover, they were discarded as solutions until the nineties, when examples of strict abnormal optimal curves were found. In order to give a detailed exposition of the proof, the paper is mostly self\textendash{}contained, which forces us to consider different areas in mathematics such as algebra, analysis, geometry.Comment: Final version. Minors changes have been made. 56 page