A numerically efficient technique for the analysis of slots in multilayer media

Cataloged from PDF version of article.A numerically efficient technique for the analysis of slot geometries in multilayer media is presented using closed-form Green's functions in spatial domain in conjunction with the method of moments (MoM). The slot is represented by an equivalent magnetic-current distribution, which is then used to determine the total power crossing through the slot and the input impedance. In order to calculate power and current distribution, spatial domain closed form Green's functions are expanded as power series of the radial distance rho, which makes the analytical evaluation of the spatial domain integrals possible, saving a considerable amount of computation time

k mer

Motivation: De novo transcriptome assembly is an integral part for many RNA-seq workflows. Common applications include sequencing of non-model organisms, cancer or meta transcriptomes. Most de novo transcriptome assemblers use the de Bruijn graph (DBG) as the underlying data structure. The quality of the assemblies produced by such assemblers is highly influenced by the exact word length k. As such no single kmer value leads to optimal results. Instead, DBGs over different kmer values are built and the assemblies are merged to improve sensitivity. However, no studies have investigated thoroughly the problem of automatically learning at which kmer value to stop the assembly. Instead a suboptimal selection of kmer values is often used in practice. Results: Here we investigate the contribution of a single kmer value in a multi-kmer based assembly approach. We find that a comparative clustering of related assemblies can be used to estimate the importance of an additional kmer assembly. Using a model fit based algorithm we predict the kmer value at which no further assemblies are necessary. Our approach is tested with different de novo assemblers for datasets with different coverage values and read lengths. Further, we suggest a simple post processing step that significantly improves the quality of multi-kmer assemblies. Conclusion: We provide an automatic method for limiting the number of kmer values without a significant loss in assembly quality but with savings in assembly time. This is a step forward to making multi-kmer methods more reliable and easier to use. Availability and Implementation:A general implementation of our approach can be found under: https://github.com/SchulzLab/KREATION. Supplementary information: Supplementary data are available at Bioinformatics online. Contact: [email protected]

Supply Chain Performance Measurement Practices of Indian Industries

In any Industry the Supply chain performance plays a crucial role and it is vital in growth of the industry. Through this study an attempt is made to find some insight to the supply chain performance measurement practices of Indian industries through an exploratory survey. The study reveals almost all the respondents (84%) felt that supply chain performance measurement system employed in their organisation has a clear purpose. Also the study reveals that most supply chain performance measurement system provides high importance to quality measurements and includes both financial and non-financial indicators. The study gave clarity in understanding the objectives of implementing supply chain performance measurement systems and metrics (measures) used in supply chain performance measurement systems The Multivariate analysis revealed three factors emerged from this study are ‘Strategic Orientation’ followed by ‘Internal Focus’ and ‘Motivation and Control’

Comparitive study of onlay and preperitoneal mesh repair in the management of ventral hernia

BRIEF RESUME OF INTENDED WORK: Ventral hernia in the anterior abdominal wall includes both spontaneous and, most commonly, incisional hernias after an abdominal operation. . Hernia recurrence is distressing to patient and embarrassing to surgeons. Mesh repair can be pre- peritoneal or onlay. Controversy exists among the surgeons regarding the use of type of either meshoplasty, due to differences in ease in performing the surgery, time of surgery, complications occurring in the post operative period and the recurrence. Only few institution do preperitoneal mesh repair due to the need of skilled surgeon, so we are comparing onlay and preperitoneal mesh repair. AIMS AND OBJECTIVES OF THE STUDY: To compare outcome of onlay and preperitoneal mesh repair in the management of ventral hernia. MATERIALS AND METHODS: METHOD OF COLLECTION OF DATA Patient admitted with ventral hernia are included in the study with details of cases, clinical examination and symptoms are included in the study after confirming the diagnosis by ultrasonography and are divided randomly into onlay and preperitoneal group with 25 patient in each group. Patient are followed for six months to study the outcome reccurence. PERIOD OF STUDY : November 2014 to April 2015 TYPE OF STUDY : Randomized control study. SOURCE OF DATA : Patient diagnosed as ventral hernia in department of general surgery, Royapettah hospital and kilpauk medical college hospital. 50 of them are to be selected on basis of non probability (purposive) sampling method. INCLUSION CRITERIA : Patient with ventral hernia including • Umbilical hernia, • Paraumblical hernia, • Epigastric hernia • Incisional hernia. EXCLUSION CRITERIA : Patient admitted with • Groin hernia , • Divarication of recti, • Recurrent hernia, • Patient medically unfit for surgery, • Obstructed and strangulated hernias. CONCLUSION : By analyzing the outcome of seroma, wound infection, flap necrosis and recurrence in both groups the final result will be submitted in my dissertation

Mafb lineage tracing to distinguish macrophages from other immune lineages reveals dual identity of Langerhans cells

Current systems for conditional gene deletion within mouse macrophage lineages are limited by ectopic activity or low efficiency. In this study, we generated a Mafb-driven Cre strain to determine whether any dendritic cells (DCs) identified by Zbtb46-GFP expression originate from a Mafb-expressing population. Lineage tracing distinguished macrophages from classical DCs, neutrophils, and B cells in all organs examined. At steady state, Langerhans cells (LCs) were lineage traced but also expressed Zbtb46-GFP, a phenotype not observed in any other population. After exposure to house dust mite antigen, Zbtb46-negative CD64(+) inflammatory cells infiltrating the lung were substantially lineage traced, but Zbtb46-positive CD64(−) cells were not. These results provide new evidence for the unique identity of LCs and challenge the notion that some inflammatory cells are a population of monocyte-derived DCs

Spinal Cord Regeneration using Stem Cell Transplantation and Other Novel Techniques

This thesis was an explorative study to evaluate potential possibilities for spinal cord regeneration. At present there is no medical treatment available to cure spinal cord injury and the person remain paralysed and incontinent for life. Based on the literature three major strategies were evaluated namely cell therapy, administration of growth factor and enzyme. All these studies were conducted in rat model of spinal cord injury. Rat olfactory mucosa isolated from the posterior region of nasal septum, and lamina propria was enzymatically separated from the epithelium. Lamina propria was enzymatically dissociated to yield olfactory ensheathing cells (OEC) and olfactory nerve fibroblast (OEC). The cells were cultured, characterized by IHC and flow cytometry to test p75NTR (OEC), fibronectin (ONF). Fresh second passage cells were labeled with lentiviral-GFP and transplanted on the 9th day following spinal cord injury with different dosage/combination into injured cord. Olfactory epithelium enzymatic digested to yield cells. These cells were cultured and Globose basal stem cells (GBC) were isolated by GBC III antibody and characterized immunohistochemically and flow cytometry methods for expression of neural stem cell marker (nestin, SOX2, NCAM), bone marrow MSC marker (CD90, CD54, CD29,CD105, CD73) and haematopoietic marker (CD45, CD34). The result showed that GBC has the properties of both neural stem cell and mesenchymal stem cell. In addition, GBC formed neurosphere in culture condition, which is the characteristic of neural stem cell in brain. These cells were neuronally induced and characterized for neuronal marker (βIII-tubulin, MAP2, NeuN, Neurofilament). The result showed invitro differentiation of GBC into neurons, and its multipotency. So this is considered as an alternative source of transplantation for spinal cord injury. Second passage GBCwere transplanted into spinal cord on day 9 following injury. Bone marrow collected from rat femur and tibia, and then isolated MSC were cultured. Cultured MSC were characterized for MSC marker (CD90, CD54, CD29, CD105, CD73) and haematopoietic marker (CD45,CD14, CD34) by flow cytometry and IHC to prove purity of MSC, not contaminated with haematopoietic cells. These cells neuronally induced and characterized for positive marker of neurons and glia (MAP2, NF, NeuN,βIII tubulin, O4 and GFAP). Mature neuron express voltage-gated sodium channel, which is the hallmark of functional excitable cells. To address this issue, voltage-gated sodium channel (Nav1.1) expression was seen by IHC and patch-clamp studies was done to prove the existence of gated sodium channel; but only K+ channel was expressed. Multipotent bone marrow mesenchymal stem cells (MSC) were lentiviral GFP labeled and then transplanted on 9th day after spinal cord injury in rat. Acidic fibroblast growth factor was administered to minimize the initial damage after spinal cord injury and inhibit secondary inflammatory cascade. The results show neuroprotective effects of aFGF. Hence, it is concluded that the combinatorial treatment for CNS injury, taking into consideration of the therapeutic value of each stem cell type tried in this study, may not provide complete cure as expected. Nevertheless, cell therapy could give a definite relief to the patient suffering from spinal cord injury. Prolonged assessment of motor recovery for a year or so after cell transplantation is beyond the scope of this study. Importantly; OEC, olfactory epithelial cells can provide an accessible source compared to intra-cranially located neural stem/progenitors for autologous neurotransplantation, eliminating the need for immunosuppression thus reducing GVHD problems. While olfactory bulb derived OECs shows remarkable regenerative potential, but practically olfactory bulb tissue harvesting is invasive for therapeutic autologous transplantation strategies. Bone marrow mesenchymal stem cell can also be isolated less invasively from iliac crest and could be a source of stem cell. Autologous neurotransplantation for spinal cord injury should be preceded by further studies in larger animals models for future clinical practice

Haematological Abnormalities in Decompensated Chronic Liver Disease

In our prevalence study, 100 patients admitted as inpatients at Government General Hospital are taken for our study to assess the hematological profile and the hemostatic profile. All the patients were evaluated for the diagnosis of cirrhosis. Then patients were subjected to investigations for the hematological profile and hemostatic profile. Patients were done liver biopsy, upper GI endoscopy, USG abdomen and clinical signs for the diagnosis of cirrhosis. Blood investigations were done to assess the anaemia, nature of anemia, WBCs total count and differential count, platelet count, prothrombin time and APTT. All the investigations were collected and tabulated. According to the study, the most common anemia in cirrhotics is normochromic normocytic anaemia. Microcytosis occur in patients with bleeding tendencies and macrocytosis occur mostly in alcoholics. Leucopenia occurs in a small fraction of patients and leucocytosis occurs in patients with history of repeated paracentesis and peritonitis. Eosinophilia is associated with parasitic infections. Thrombocytopenia is present in most of the cirrhosis patients and are associated with increased bleeding tendencies. Most of the patients had increased prothrombin time and APTT due to decreased synthesis of clotting factors. Thus in cirrhosis patients most of them had abnormalities in haematological parameters and hemostasis