How will disenfranchised Peoples adapt to Climate Change? Strengthening the Ecojustice Movement

The Fourth assessment of the Intergovernmental Panel on Climate Change (IPCC) acknowledged That millions of people are currently, and will increasingly be, affected by the impacts of climate change, in the form of floods, droughts and other extreme events, as well as related threats to food security. In response to these global environmental changes, the international community, including civil society, is acting on the need for immediate adaptation measures and is developing strategies for future adaptation. However, the impacts of climate change are unevenly distributed, with many of the poorest, most vulnerable peoples experiencing the immediate effects of climate change, in the here and now. As the IPCC noted, developing countries are disproportionately affected by climate change and often, the least able to adapt due to lack of infrastructure and resources

The Drosophila Anion Exchanger (DAE) lacks a detectable interaction with the spectrin cytoskeleton

<p>Abstract</p> <p>Background</p> <p>Current models suggest that the spectrin cytoskeleton stabilizes interacting ion transport proteins at the plasma membrane. The human erythrocyte anion exchanger (AE1) was the first membrane transport protein found to be associated with the spectrin cytoskeleton. Here we evaluated a conserved anion exchanger from Drosophila (DAE) as a marker for studies of the downstream effects of spectrin cytoskeleton mutations.</p> <p>Results</p> <p>Sequence comparisons established that DAE belongs to the SLC4A1-3 subfamily of anion exchangers that includes human AE1. Striking sequence conservation was observed in the C-terminal membrane transport domain and parts of the N-terminal cytoplasmic domain, but not in the proposed ankyrin-binding site. Using an antibody raised against DAE and a recombinant transgene expressed in <it>Drosophila </it>S2 cells DAE was shown to be a 136 kd plasma membrane protein. A major site of expression was found in the stomach acid-secreting region of the larval midgut. DAE codistributed with an infolded subcompartment of the basal plasma membrane of interstitial cells. However, spectrin did not codistribute with DAE at this site or in anterior midgut cells that abundantly expressed both spectrin and DAE. Ubiquitous knockdown of DAE with dsRNA eliminated antibody staining and was lethal, indicating that DAE is an essential gene product in <it>Drosophila</it>.</p> <p>Conclusions</p> <p>Based on the lack of colocalization and the lack of sequence conservation at the ankyrin-binding site, it appears that the well-characterized interaction between AE1 and the spectrin cytoskeleton in erythrocytes is not conserved in <it>Drosophila</it>. The results establish a pattern in which most of the known interactions between the spectrin cytoskeleton and the plasma membrane in mammals do not appear to be conserved in <it>Drosophila</it>.</p

The warm interstellar medium around the Cygnus Loop

Observations of the oxygen lines [OII]3729 and [OIII]5007 in the medium immediately beyond the Cygnus Loop supernova remnant were carried out with the scanning Fabry-P\'erot spectrophotometer ESOP. Both lines were detected in three different directions - east, northeast and southwest - and up to a distance of 15 pc from the shock front. The ionized medium is in the immediate vicinity of the remnant, as evinced by the smooth brightening of both lines as the adiabatic shock transition (defined by the X-ray perimeter) is crossed. These lines are usually brighter around the Cygnus Loop than in the general background in directions where the galactic latitude is above 5 degrees. There is also marginal (but significant) evidence that the degree of ionization is somewhat larger around the Cygnus Loop. We conclude that the energy necessary to ionize this large bubble of gas could have been supplied by an O8 or O9 type progenitor or the particles heated by the expanding shock front. The second possibility, though highly atractive, would have to be assessed by extensive modelling.Comment: 18 pages, 8 figures, ApJ 512 in pres

Rho activation patterns after spinal cord injury and the role of activated Rho in apoptosis in the central nervous system

Growth inhibitory proteins in the central nervous system (CNS) block axon growth and regeneration by signaling to Rho, an intracellular GTPase. It is not known how CNS trauma affects the expression and activation of RhoA. Here we detect GTP-bound RhoA in spinal cord homogenates and report that spinal cord injury (SCI) in both rats and mice activates RhoA over 10-fold in the absence of changes in RhoA expression. In situ Rho-GTP detection revealed that both neurons and glial cells showed Rho activation at SCI lesion sites. Application of a Rho antagonist (C3–05) reversed Rho activation and reduced the number of TUNEL-labeled cells by ∼50% in both injured mouse and rat, showing a role for activated Rho in cell death after CNS injury. Next, we examined the role of the p75 neurotrophin receptor (p75NTR) in Rho signaling. After SCI, an up-regulation of p75NTR was detected by Western blot and observed in both neurons and glia. Treatment with C3–05 blocked the increase in p75NTR expression. Experiments with p75NTR-null mutant mice showed that immediate Rho activation after SCI is p75NTR dependent. Our results indicate that blocking overactivation of Rho after SCI protects cells from p75NTR-dependent apoptosis

Особенности развития гаптофитовых и динофитовых водорослей в олигоценовых бассейнах Северного Перитетиса

На основании изучения наннопланктона и диноцист проведены палеоэкологические реконструкции разных типов олигоценовых бассейнов Северного Перитетиса. Установлены ассоциации фитопланктона морских глубоководных, мелководных, относительно холодно- и тепловодных, а также лагунных палеобассейнов. Гаптофитовые (наннопланктон) присутствуют только в карбонатных прослоях пород, наиболее благоприятные условия для их развития были в Карпатском бассейне кросненского типа, в бассейнах самого раннего рюпеля Германии и юга Украины. Диноцисты представлены во всех типах олигоценовых бассейнов. Установлены корреляционные уровни по нанно- и динопланктону, позволяющие обосновать нижнюю и верхнюю границы олигоцена и уровень опреснения в середине рюпеля.Вивчення нанопланктону та диноцист з різних типів олігоценових басейнів Північного Перитетіса дозволило провести палеоекологічні реконструкції. Встановлені асоціації фітопланктону морських глибоководних, прибережно-мілководних відносно холодно- и тепловодних, лагунних, напівізольованих з ендеміками. Нанопланктон виявлено лише в карбонатних прошарках, найбільш сприятливі умови для його розвитку були у Карпатському басейні кросненського типу, у самому ранньому рюпелі Німеччини та півдня України. Диноцисти виявлені у всіх типах олігоценових басейнів. Встановлені корелятивні рівні за нано- та динопланктоном, які дозволяють обґрунтувати нижню і верхню границі олігоцену та рівень розпріснення в середині рюпелю.A study of nannofossils and dinocysts from different types of Oligocene basins of the Northern Peri-Thetys resulted in paleoecological reconstructions. The following phytoplankton associations were recognized: cold-water; littoral shallow-water relatively cold- and warm-water; lagoon; semi-isolated with endemics. Nannofossils were present in carbonaceous sediments, with more favorable conditions for them being in the Carpathian basin of Krosnensky type, in the earliest Rupelian of Germany and Southern Ukraine. Dinocysts were identified in all types of Oligocene basins. Correlation levels identified by nannofossils and dinocysts allowed us to substantiate the lower and upper Oligocene boundaries and degree of desalination in the Middle Rupelian

Remote Monitoring of Fish in Small Streams: A Unified Approach Using Pit Tags

Accurate assessments of fish populations are often limited by re-observation or recapture events. Since the early 1990s, passive integrated transponders (PIT tags) have been used to understand the biology of many fish species. Until recently, PIT applications in small streams have been limited to physical recapture events. To maximize recapture probability, we constructed PIT antenna arrays in small streams to remotely detect individual fish. Experiences from two different laboratories (three case studies) allowed us to develop a unified approach to applying PIT technology for enhancing data assessments. Information on equipment, its installation, tag considerations, and array construction is provided. Theoretical and practical definitions are introduced to standardize metrics for assessing detection efficiency. We demonstrate how certain conditions (stream discharge, vibration, and ambient radio frequency noise) affect the detection efficiency and suggest that by monitoring these conditions, expectations of efficiency can be modified. We emphasize the importance of consistently estimating detection efficiency for fisheries applications

Effect of the Structure of Amido-polynitrogen Molecules on the Complexation of Actinides

AbstractThe complexation and solvent extraction of Eu(III) and actinides in different oxidation states (Am(III), Pu(IV), Np(V)) by bitopic molecules with a dipyridyl-phenanthroline cycle as nitrogen unit and one or two amido functions are described. The complexation has been studied in methanol-water solution with hydrophilic molecules to enhance knowledge about this new family of ligands and to identify the most interesting structural effect. Some extraction tests have been performed with lipophilic molecules of the family to check the possible utility of the new class of ligands under representative fuel reprocessing conditions. These first studies have demonstrated that the presence of a preorganized N-donors unit like dipyridyl-phenanthroline improves the ligand's affinity for actinides and its An/Ln selectivity

Study of timing characteristics of a 3 m long plastic scintillator counter using waveform digitizers

A plastic scintillator bar with dimensions 300 cm x 2.5 cm x 11 cm was exposed to a focused muon beam to study its light yield and timing characteristics as a function of position and angle of incidence. The scintillating light was read out at both ends by photomultiplier tubes whose pulse shapes were recorded by waveform digitizers. Results obtained with the WAVECATCHER and SAMPIC digitizers are analyzed and compared. A discussion of the various factors affecting the timing resolution is presented. Prospects for applications of plastic scintillator technology in large-scale particle physics detectors with timing resolution around 100 ps are provided in light of the results