In vitro cryopreservation of date palm caulogenic meristems

Cryopreservation is the technology of choice not only for plant genetic resource preservation but also for virus eradication and for the efficient management of large-scale micropropagation. In this chapter, we describe three cryopreservation protocols (standard vitrification, droplet vitrification, and encapsulation vitrification) for date palm highly proliferating meristems that are initiated from vitro-cultures using plant growth regulator-free MS medium. The positive impact of sucrose preculture and cold hardening treatments on survival rates is significant. Regeneration rates obtained with standard vitrification, encapsulation-vitrification, and droplet-vitrification protocols can reach 30, 40, and 70%, respectively. All regenerated plants from non-cryopreserved or cryopreserved explants don't show morphological variation by maintaining genetic integrity without adverse effect of cryogenic treatment. Cryopreservation of date palm vitro-cultures enables commercial tissue culture laboratories to move to large-scale propagation from cryopreserved cell lines producing true-to-type plants after clonal field-testing trials. When comparing the cost of cryostorage and in-field conservation of date palm cultivars, tissue cryopreservation is the most cost-effective. Moreover, many of the risks linked to field conservation like erosion due to climatic, edaphic, and phytopathologic constraints are circumvented. (Résumé d'auteur

Enhanced Graph Rewriting Systems for Complex Software Domain

International audienceMethodologies for correct by construction reconfigurations can efficiently solve consistency issues in dynamic software architecture. Graph-based models are appropriate for designing such architectures and methods. At the same time, they may be unfit to characterize a system from a non functional perspective. This stems from efficiency and applicability limitations in handling time-varying characteristics and their related dependencies. In order to lift these restrictions, an extension to graph rewriting systems is proposed herein. The suitability of this approach, as well as the restraints of currently available ones, are illustrated, analysed and experimentally evaluated with reference to a concrete example. This investigation demonstrates that the conceived solution can: (i) express any kind of algebraic dependencies between evolving requirements and properties; (ii) significantly ameliorate the efficiency and scalability of system modifications with respect to classic methodologies; (iii) provide an efficient access to attribute values; (iv) be fruitfully exploited in software management systems; (v) guarantee theoretical properties of a grammar, like its termination

Genetic diversity at the Dhn3 locus in Turkish Hordeum spontaneum populations with comparative structural analyses

We analysed Hordeum spontaneum accessions from 21 different locations to understand the genetic diversity of HsDhn3 alleles and effects of single base mutations on the intrinsically disordered structure of the resulting polypeptide (HsDHN3). HsDHN3 was found to be YSK2-type with a low-frequency 6-aa deletion in the beginning of Exon 1. There is relatively high diversity in the intron region of HsDhn3 compared to the two exon regions. We have found subtle differences in K segments led to changes in amino acids chemical properties. Predictions for protein interaction profiles suggest the presence of a protein-binding site in HsDHN3 that coincides with the K1 segment. Comparison of DHN3 to closely related cereals showed that all of them contain a nuclear localization signal sequence flanking to the K1 segment and a novel conserved region located between the S and K1 segments [E(D/T)DGMGGR]. We found that H. vulgare, H. spontaneum, and Triticum urartu DHN3s have a greater number of phosphorylation sites for protein kinase C than other cereal species, which may be related to stress adaptation. Our results show that the nature and extent of mutations in the conserved segments of K1 and K2 are likely to be key factors in protection of cells

ISO-SWS calibration and the accurate modelling of cool-star atmospheres - IV. G9 to M2 stars

presented. The 2.38 -- 4.08 micron wavelength-range of band 1 of ISO-SWS (Short-Wavelength Spectrometers on board of the Infrared Space Observatory) in which many different molecules -- with their own dependence on each of the stellar parameters -- are absorbing, enables us to estimate the effective temperature, the gravity, the microturbulence, the metallicity, the CNO-abundances, the 12C/13C-ratio and the angular diameter from the ISO-SWS data. Using the Hipparcos' parallax, the radius, luminosity and gravity-inferred mass are derived. The stellar parameters obtained are in good agreement with other published values, though also some discrepancies with values deduced by other authors are noted. For a few stars (Delta Dra, Xi Dra, Alpha Tuc, H Sco and Alpha Cet) some parameters -- e.g. the CNO-abundances -- are derived for the first time. By examining the correspondence between different ISO-SWS observations of the same object and between the ISO-SWS data and the corresponding synthetic spectrum, it is shown that the relative accuracy of ISO-SWS in band 1 (2.38 -- 4.0 micron) is better than 2% for these high-flux sources. The high level of correspondence between observations and theoretical predictions, together with a confrontation of the estimated Teff(ISO) value with Teff-values derived from colours -- which demonstrates the consistency between V-K, BC(K), Teff and the angular diameter derived from optical or IR data -- proves that both the used MARCS models to derive the stellar quantities and the flux calibration of the ISO-SWS detectors have reached a high level of reliability.Comment: 19 pages, 15 figures; Astronomy and Astrophysics, in press; preprints can be obtained by contacting [email protected] or via WWW on http://www.ster.kuleuven.ac.be/~leen or via anonymous ftp on ftp://ftp.ster.kuleuven.ac.be/dist/leen/latex/h3318 Appendix electronically available (26 pages, 22 figures

Distribution of microplankton in relation to environmental variables during summer stratification in the Gulf of Hammamet, Tunisia

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