Strength distribution of solar magnetic fields in photospheric quiet Sun regions

The magnetic topology of the solar photosphere in its quietest regions is hidden by the difficulties to disentangle magnetic flux through the resolution element from the field strength of unresolved structures. The observation of spectral lines with strong coupling with hyperfine structure, like the observed MnI line at 553.7 nm, allows such differentiation. The main aim is to analyse the distribution of field strengths in the network and intranetwork of the solar photosphere through inversion of the MnI line at 553.7 nm. An inversion code for the magnetic field using the Principal Component Analysis (PCA) has been developed. Statistical tests are run on the code to validate it. The code has to draw information from the small-amplitude spectral feature oppearing in the core of the Stokes V profile of the observed line for field strengths below a certain threshold, coinciding with lower limit of the Paschen-Back effect in the fine structure of the involved atomic levels. The inversion of the observed profiles, using the circular polarization (V) and the intensity (I), shows the presence of magnetic fields strengths in a range from 0 to 2 kG, with predominant weak strength values. Mixed regions with mean strength field values of 1130 and 435 Gauss are found associated with the network and intranetwork respectively. The MnI line at 553 nm probes the field strength distribution in the quiet sun and shows the predominance of weak, hectoGauss fields in the intranetwork, and strong, kiloGauss fields in the network. It also shows that both network and intranetwork are to be understood at our present spatial resolutions as field distributions of which we hint the mean properties.Comment: 10 pages, 6 figure

Survey on the management of Pompe disease in routine clinical practice in Spain

Antibodies; Diagnosis; Pompe diseaseAnticuerpos; Diagnóstico; Enfermedad de pompeAnticossos; Diagnòstic; Malaltia de pompeBackground Despite the availability of several clinical guidelines, not all health professionals use their recommendations to manage patients with Pompe disease, a rare genetic disorder involving high-impact therapy. Through several discussion meetings and a survey, the present study aimed to learn about the management of Pompe disease in routine clinical practice in Spain, to improve clinical care in a real-life situation. Results The survey was sent to 42 healthcare professionals who manage patients with Pompe disease in their clinical practice. Although most respondents followed the clinical guidelines, clinical practice differed from the expert recommendations in many cases. Approximately 7% did not request a genetic study to confirm the diagnosis before starting treatment, and 21% considered that only two dried blood spot determinations suffice to establish the diagnosis. About 76% requested anti-GAA antibodies when there is a suspicion of lack of treatment efficacy, though a significant percentage of respondents have never requested such antibodies. According to 31% of the respondents, significant impairment of motor function and/or respiratory insufficiency is a requirement for authorizing medication at their hospital. Up to 26% waited for improvements over the clinical follow-up to maintain treatment and withdrew it in the absence of improvement since they did not consider disease stabilization to be a satisfactory outcome. Conclusions The results highlight the lack of experience and/or knowledge of some professionals caring for patients with Pompe disease. It is necessary to develop and disseminate simple guidelines that help to apply the expert recommendations better or centralize patient follow-up in highly specialized centers.Sanofi has sponsored this project without participating in the article’s design, data analysis, or writing

Identification of Potential Muscle Biomarkers in McArdle Disease: Insights from Muscle Proteome Analysis

McArdle disease; Proteomics; Skeletal muscleEnfermedad de McArdle; Proteómica; Músculo esqueléticoMalaltia de McArdle; Proteòmica; Múscul esquelèticGlycogen storage disease type V (GSDV, McArdle disease) is a rare genetic myopathy caused by deficiency of the muscle isoform of glycogen phosphorylase (PYGM). This results in a block in the use of muscle glycogen as an energetic substrate, with subsequent exercise intolerance. The pathobiology of GSDV is still not fully understood, especially with regard to some features such as persistent muscle damage (i.e., even without prior exercise). We aimed at identifying potential muscle protein biomarkers of GSDV by analyzing the muscle proteome and the molecular networks associated with muscle dysfunction in these patients. Muscle biopsies from eight patients and eight healthy controls showing none of the features of McArdle disease, such as frequent contractures and persistent muscle damage, were studied by quantitative protein expression using isobaric tags for relative and absolute quantitation (iTRAQ) followed by artificial neuronal networks (ANNs) and topology analysis. Protein candidate validation was performed by Western blot. Several proteins predominantly involved in the process of muscle contraction and/or calcium homeostasis, such as myosin, sarcoplasmic/endoplasmic reticulum calcium ATPase 1, tropomyosin alpha-1 chain, troponin isoforms, and alpha-actinin-3, showed significantly lower expression levels in the muscle of GSDV patients. These proteins could be potential biomarkers of the persistent muscle damage in the absence of prior exertion reported in GSDV patients. Further studies are needed to elucidate the molecular mechanisms by which PYGM controls the expression of these proteins.This research was funded by Instituto de Salud Carlos III (ISCIII) y FEDER (ERDF) funds “a way to construct Europe”; Ministerio de Ciencia e Innovación (Madrid, Spain), grant numbers (PI17/02052 and PI19/01313). G.N.-G is supported by a ISCIII contract CPII19/00021. P.S.-L. is supported by a ISCIII-CIBERER contract

Are beryllium abundances anomalous in stars with giant planets?

In this paper we present beryllium (Be) abundances in a large sample of 41 extra-solar planet host stars, and for 29 stars without any known planetary-mass companion, spanning a large range of effective temperatures. The Be abundances were derived through spectral synthesis done in standard Local Thermodynamic Equilibrium, using spectra obtained with various instruments. The results seem to confirm that overall, planet-host stars have ``normal'' Be abundances, although a small, but not significant, difference might be present. This result is discussed, and we show that this difference is probably not due to any stellar ``pollution'' events. In other words, our results support the idea that the high-metal content of planet-host stars has, overall, a ``primordial'' origin. However, we also find a small subset of planet-host late-F and early-G dwarfs that might have higher than average Be abundances. The reason for the offset is not clear, and might be related either to the engulfment of planetary material, to galactic chemical evolution effects, or to stellar-mass differences for stars of similar temperature.Comment: 15 pages, 9 figures, accepted for publication in Astronomy & Astrophysic

EVIDENT 3 Study: A randomized, controlled clinical trial to reduce inactivity and caloric intake in sedentary and overweight or obese people using a smartphone application: Study protocol

Introduction: Mobile technology, when included within multicomponent interventions, could contribute to more effective weight loss. The objective of this project is to assess the impact of adding the use of the EVIDENT 3 application, designed to promote healthy living habits, to traditional modification strategies employed for weight loss. Other targeted behaviors (walking, caloric-intake, sitting time) and outcomes (quality of life, inflammatory markers, measurements of arterial aging) will also be evaluated. Methods: Randomized, multicentre clinical trial with 2 parallel groups. The study will be conducted in the primary care setting and will include 700 subjects 20 to 65 years, with a body mass index (27.5-40kg/m2), who are clinically classified as sedentary. The primary outcome will be weight loss. Secondary outcomes will include change in walking (steps/d), sitting time (min/wk), caloric intake (kcal/d), quality of life, arterial aging (augmentation index), and pro-inflammatory marker levels. Outcomes will be measured at baseline, after 3 months, and after 1 year. Participants will be randomly assigned to either the intervention group (IG) or the control group (CG). Both groups will receive the traditional primary care lifestyle counseling prior to randomization. The subjects in the IG will be lent a smartphone and a smartband for a 3-month period, corresponding to the length of the intervention. The EVIDENT 3 application integrates the information collected by the smartband on physical activity and the self-reported information by participants on daily food intake. Using this information, the application generates recommendations and personalized goals for weight loss. Discussion: There is a great diversity in the applications used obtaining different results on lifestyle improvement and weight loss. The populations studied are not homogeneous and generate different results. The results of this study will help our understanding of the efficacy of new technologies, combined with traditional counseling, towards reducing obesity and enabling healthier lifestyles. Ethicsanddissemination: The study was approved by the Clinical Research Ethics Committee of the Health Area of Salamanca ("CREC of Health Area of Salamanca") on April 2016. A SPIRIT checklist is available for this protocol. The trial was registered in ClinicalTrials.gov provided by the US National Library of Medicine-number NCT03175614

Enhanced lithium depletion in Sun-like stars with orbiting planets

The surface abundance of lithium on the Sun is 140 times less than protosolar, yet the temperature at the base of the surface convective zone is not hot enough to burn Li. A large range of Li abundances in solar type stars of the same age, mass and metallicity is observed, but theoretically difficult to understand. An earlier suggestion that Li is more depleted in stars with planets was weakened by the lack of a proper comparison sample of stars without detected planets. Here we report Li abundances for an unbiased sample of solar-analogue stars with and without detected planets. We find that the planet-bearing stars have less than 1 per cent of the primordial Li abundance, while about 50 per cent of the solar analogues without detected planets have on average 10 times more Li. The presence of planets may increase the amount of mixing and deepen the convective zone to such an extent that the Li can be burned.Comment: 13 pages, 2 figure

Lithium-rich giants in the Galactic thick disk

Context: Lithium is a fragile element, which is easily destroyed in the stellar interior. The existence of lithium-rich giants still represents a challenge for stellar evolution models. Aims: We have collected a large database of high-resolution stellar spectra of 824 candidate thick-disk giants having 2\,MASS photometry and proper motions measured by the Southern Proper-Motion Program (SPM). In order to investigate the nature of Li-rich giants, we searched this database for giants presenting a strong Li\,I resonance line. Methods: We performed a chemical abundance analysis on the selected stars with the MOOG code along with proper ATLAS-9 model atmospheres. The iron content and atmospheric parameters were fixed by using the equivalent width of a sample of Fe lines. We also derive abundances for C, N, and O and measure or derive lower limits on the $^{12}$C/$^{13}$C isotopic ratios, which is a sensible diagnostic of the stars evolutionary status. Results: We detected five stars with a lithium abundance higher than 1.5, i.e. Li-rich according to the current definition. One of them (SPM-313132) has A(Li)$>$3.3 and, because of this, belongs to the group of the rare super Li-rich giants. Its kinematics makes it a likely thin-disk member and its atmospheric parameters are compatible with it being a 4\,M$_\odot$ star either on the red giant branch (RGB) or the early asymptotic giant branch. This object is the first super Li-rich giant detected at this phase. The other four are likely low-mass thick-disk stars evolved past the RGB luminosity bump, as determined from their metallicities and atmospheric parameters. The most evolved of them lies close to the RGB-tip. It has A(Li)$>$2.7 and a low $^{12}$C/$^{13}$C isotopic ratio, close to the cool bottom processing predictions.Comment: 11 pages, 7 tables, 7 figures. Accepted for publication in A&

Local helioseismology of sunspot regions: comparison of ring-diagram and time-distance results

Local helioseismology provides unique information about the subsurface structure and dynamics of sunspots and active regions. However, because of complexity of sunspot regions local helioseismology diagnostics require careful analysis of systematic uncertainties and physical interpretation of the inversion results. We present new results of comparison of the ring-diagram analysis and time-distance helioseismology for active region NOAA 9787, for which a previous comparison showed significant differences in the subsurface sound-speed structure, and discuss systematic uncertainties of the measurements and inversions. Our results show that both the ring-diagram and time-distance techniques give qualitatively similar results, revealing a characteristic two-layer seismic sound-speed structure consistent with the results for other active regions. However, a quantitative comparison of the inversion results is not straightforward. It must take into account differences in the sensitivity, spatial resolution and the averaging kernels. In particular, because of the acoustic power suppression, the contribution of the sunspot seismic structure to the ring-diagram signal can be substantially reduced. We show that taking into account this effect reduces the difference in the depth of transition between the negative and positive sound-speed variations inferred by these methods. Further detailed analysis of the sensitivity, resolution and averaging properties of the local helioseismology methods is necessary for consolidation of the inversion results. It seems to be important that both methods indicate that the seismic structure of sunspots is rather deep and extends to at least 20 Mm below the surface, putting constraints on theoretical models of sunspots.Comment: 10 pages, 10 figures, submitted to Journal of Physics: Conference Series (JPCS) GONG 2010 - SoHO 24 "A new era of seismology of the Sun and solar-like stars", June 27 - July 2, 2010 Aix-en-Provence, Franc

Formation of left-handed helices by C2′-fluorinated nucleic acids under physiological salt conditions

Recent findings in cell biology have rekindled interest in Z-DNA, the left-handed helical form of DNA. We report here that two minimally modified nucleosides, 2'F-araC and 2'F-riboG, induce the formation of the Z-form under low ionic strength. We show that oligomers entirely made of these two nucleosides exclusively produce left-handed duplexes that bind to the Zα domain of ADAR1. The effect of the two nucleotides is so dramatic that Z-form duplexes are the only species observed in 10 mM sodium phosphate buffer and neutral pH, and no B-form is observed at any temperature. Hence, in contrast to other studies reporting formation of Z/B-form equilibria by a preference for purine glycosidic angles in syn, our NMR and computational work revealed that sequential 2'F…H2N and intramolecular 3'H…N3' interactions stabilize the left-handed helix. The equilibrium between B- and Z- forms is slow in the 19F NMR time scale (≥ms), and each conformation exhibited unprecedented chemical shift differences in the 19F signals. This observation led to a reliable estimation of the relative population of B and Z species and enabled us to monitor B-Z transitions under different conditions. The unique features of 2'F-modified DNA should thus be a valuable addition to existing techniques for specific detection of new Z-binding proteins and ligands