Ethanol exposure perturbs sea urchin development and disrupts developmental timing

Ethanol is a known vertebrate teratogen that causes craniofacial defects as a component of fetal alcohol syndrome (FAS). Our results show that sea urchin embryos treated with ethanol similarly show broad skeletal patterning defects, potentially analogous to the defects associated with FAS. The sea urchin larval skeleton is a simple patterning system that involves only two cell types: the primary mesenchymal cells (PMCs) that secrete the calcium carbonate skeleton and the ectodermal cells that provide migratory, positional, and differentiation cues for the PMCs. Perturbations in RA biosynthesis and Hh signaling pathways are thought to be causal for the FAS phenotype in vertebrates. Surprisingly, our results indicate that these pathways are not functionally relevant for the teratogenic effects of ethanol in developing sea urchins. We found that developmental morphology as well as the expression of ectodermal and PMC genes was delayed by ethanol exposure. Temporal transcriptome analysis revealed significant impacts of ethanol on signaling and metabolic gene expression, and a disruption in the timing of GRN gene expression that includes both delayed and precocious gene expression throughout the specification network. We conclude that the skeletal patterning perturbations in ethanol-treated embryos likely arise from a loss of temporal synchrony within and between the instructive and responsive tissues.IOS-1656752 - National Science Foundation; National Science FoundationFirst author draf

Sustaining The Saco Estuary: Final Report 2015

This study focuses on the Saco estuary, the tidal portion of the Saco River, which drains the largest watershed in southern Maine. With headwaters in the White Mountains of New Hampshire, the watershed encompasses more than 4,400 km2, and provides clean healthy drinking water to over 100,000 people living and working in communities in southern Maine. When the study began in 2009, very little was known about the ecology of the Saco estuary. Researchers at the University of New England and the Wells National Estuarine Research Reserve employed the process of collaborative learning to bring together people who care about the estuary in order to identify their concerns. A Stewardship Network composed of people employed by municipal, state and federal governments, water supply organizations and businesses, volunteers from municipal boards making land use decisions, land trusts, property owners and representatives from other organizations that are uniquely focused on the region was formed. The Stewardship Network helped to define the project goals and objectives, and provided input and guidance over the five-year project. This report explains what the researchers discovered about the ecology of the estuary, along with what they learned about its social and economic components. This baseline assessment contributes to the long-term goal of restoring and sustaining the structure and function of the estuary, and supports the efforts of government, businesses and local organizations that value the estuary and depend upon the natural services it provides

Clinically Suspected Myocarditis Temporally Related to COVID-19 Vaccination in Adolescents and Young Adults: Suspected Myocarditis After COVID-19 Vaccination

Background: Understanding the clinical course and short-term outcomes of suspected myocarditis after the coronavirus disease 2019 (COVID-19) vaccination has important public health implications in the decision to vaccinate youth. Methods: We retrospectively collected data on patients <21 years old presenting before July 4, 2021, with suspected myocarditis within 30 days of COVID-19 vaccination. Lake Louise criteria were used for cardiac MRI findings. Myocarditis cases were classified as confirmed or probable on the basis of the Centers for Disease Control and Prevention definitions. Results: We report on 139 adolescents and young adults with 140 episodes of suspected myocarditis (49 confirmed, 91 probable) at 26 centers. Most patients were male (n=126, 90.6%) and White (n=92, 66.2%); 29 (20.9%) were Hispanic; and the median age was 15.8 years (range, 12.1–20.3; interquartile range [IQR], 14.5–17.0). Suspected myocarditis occurred in 136 patients (97.8%) after the mRNA vaccine, with 131 (94.2%) after the Pfizer-BioNTech vaccine; 128 (91.4%) occurred after the second dose. Symptoms started at a median of 2 days (range, 0–22; IQR, 1–3) after vaccination. The most common symptom was chest pain (99.3%). Patients were treated with nonsteroidal anti-inflammatory drugs (81.3%), intravenous immunoglobulin (21.6%), glucocorticoids (21.6%), colchicine (7.9%), or no anti-inflammatory therapies (8.6%). Twenty-six patients (18.7%) were in the intensive care unit, 2 were treated with inotropic/vasoactive support, and none required extracorporeal membrane oxygenation or died. Median hospital stay was 2 days (range, 0–10; IQR, 2–3). All patients had elevated troponin I (n=111, 8.12 ng/mL; IQR, 3.50–15.90) or T (n=28, 0.61 ng/mL; IQR, 0.25–1.30); 69.8% had abnormal ECGs and arrhythmias (7 with nonsustained ventricular tachycardia); and 18.7% had left ventricular ejection fraction <55% on echocardiogram. Of 97 patients who underwent cardiac MRI at a median 5 days (range, 0–88; IQR, 3–17) from symptom onset, 75 (77.3%) had abnormal findings: 74 (76.3%) had late gadolinium enhancement, 54 (55.7%) had myocardial edema, and 49 (50.5%) met Lake Louise criteria. Among 26 patients with left ventricular ejection fraction <55% on echocardiogram, all with follow-up had normalized function (n=25). Conclusions: Most cases of suspected COVID-19 vaccine myocarditis occurring in persons <21 years have a mild clinical course with rapid resolution of symptoms. Abnormal findings on cardiac MRI were frequent. Future studies should evaluate risk factors, mechanisms, and long-term outcomes

A 25-year perspective on levels of analysis in leadership research

The purpose of this article is to present a comprehensive 25-year review of the incorporation of levels of analysis into conceptual and empirical leadership research published within Leadership Quarterly throughout its history. We assessed the population of Leadership Quarterly's research (790 research articles) on four key levels of analysis-based issues: (1) explicit statement of the focal level(s) of analysis; (2) appropriate measurement given level of constructs; (3) use of a multi-level data analysis technique; and, (4) alignment of theory and data. Prior reviews regarding levels of analysis incorporation into leadership research have been limited to major research domains. Results revealed that while both conceptual and empirical articles only explicitly state the focal level of analysis in approximately one-third of the articles, appropriate levels-based measurement and alignment between theory and data are relatively strong areas of achievement for the articles within Leadership Quarterly. Multi-level data analysis techniques are used in less than one-fifth of all articles. Although there is room for improvement, there is evidence that Leadership Quarterly is a premier outlet for levels-based leadership research. Given the increasing complexity of organizational science with regard to groups, teams and collectives, Leadership Quarterly has an opportunity to model for organizational research on how to build and test complicated multi-level theories and models

Leadership emergence in face-to-face and virtual teams : a multi-level model with agent-based simulations, quasi-experimental and experimental tests

With leadership as a major predictor of team performance in both face-to-face and virtual teams, research on differences in leadership emergence in these contexts seems warranted. We offer a multi-level model analyzing the roles of degree of team virtuality and density of social network ties as boundary conditions on leadership emergence, viewed as a fundamentally social–cognitive process. Using agent-based modeling and simulations, our results suggest that virtuality moderates the relationships between cognitive ability, extraversion, and self-efficacy (as independent variables) and leadership emergence (as dependent variable); and density of network ties serves as a moderator for the associations of cognitive ability and self-efficacy with leadership emergence. Subsequent quasi-experimental and experimental tests support the role of density of network ties as a moderator for the association of extraversion with leadership emergence. Implications of these findings and future paths for research bridging the fields of leadership, team virtuality and social networks are discussed

The 6dF Galaxy Survey: samples, observational techniques and the first data release

The 6dF Galaxy Survey (6dFGS) aims to measure the redshifts of around 150,000 galaxies, and the peculiar velocities of a 15,000-member sub-sample, over almost the entire southern sky. When complete, it will be the largest redshift survey of the nearby universe, reaching out to about z ~ 0.15, and more than an order of magnitude larger than any peculiar velocity survey to date. The targets are all galaxies brighter than K_tot = 12.75 in the 2MASS Extended Source Catalog (XSC), supplemented by 2MASS and SuperCOSMOS galaxies that complete the sample to limits of (H, J, r_F, b_J) = (13.05, 13.75, 15.6, 16.75). Central to the survey is the Six-Degree Field (6dF) multi-fibre spectrograph, an instrument able to record 150 simultaneous spectra over the 5.7-degree field of the UK Schmidt Telescope. An adaptive tiling algorithm has been employed to ensure around 95% fibering completeness over the 17046 sq.deg of the southern sky with | b | > 10 deg. Spectra are obtained in two observations using separate V and R gratings, that together give R ~ 1000 over at least 4000 -- 7500 Angstroms and signal-to-noise ratio ~10 per pixel. The 6dFGS database is available at http://www-wfau.roe.ac.uk/6dFGS/, with public data releases occuring after the completion of each third of the survey.Comment: 17 pages, 10 figures, submitted to MNRAS. Full resolution version of the paper available at http://www-wfau.roe.ac.uk/6dFGS/ and http://www.mso.anu.edu.au/6dFGS

Single-nucleus cross-tissue molecular reference maps toward understanding disease gene function

Understanding gene function and regulation in homeostasis and disease requires knowledge of the cellular and tissue contexts in which genes are expressed. Here, we applied four single-nucleus RNA sequencing methods to eight diverse, archived, frozen tissue types from 16 donors and 25 samples, generating a cross-tissue atlas of 209,126 nuclei profiles, which we integrated across tissues, donors, and laboratory methods with a conditional variational autoencoder. Using the resulting cross-tissue atlas, we highlight shared and tissue-specific features of tissue-resident cell populations; identify cell types that might contribute to neuromuscular, metabolic, and immune components of monogenic diseases and the biological processes involved in their pathology; and determine cell types and gene modules that might underlie disease mechanisms for complex traits analyzed by genome-wide association studies.</jats:p