DISEÑO Y CONSTRUCCIÓN DE UN SISTEMA PROTOTIPO PARA EL APROVECHAMIENTO DE BIOGAS EN EL PROCESO DE CURADO DE TABACO VIRGINIA PARA HORNOS FLUE CURED

En este artículo se presentan los resultados del proyecto “Diseño y construcción de un sistema prototipo para el aprovechamiento de biogás en el proceso de curado de tabaco virginia para hornos Flue Cured en los departamentos de Santander y Huila.” Este proyecto está dirigido a la Cadena Productiva del Tabaco para el aprovechamiento energético a partir de un sistema de generación y compresión de Biogás en los proceso de curado de Tabaco Virginia. El objeto del proyecto es plantear una solución energética alternativa que ofrezca de forma particular y muy creativa solución al problema de los altos costos de producción por la utilización de los actuales combustibles, mitigando los actuales problemas ambientales por las emisiones generadas por los mismos durante el proceso

Treatment with tocilizumab or corticosteroids for COVID-19 patients with hyperinflammatory state: a multicentre cohort study (SAM-COVID-19)

Objectives: The objective of this study was to estimate the association between tocilizumab or corticosteroids and the risk of intubation or death in patients with coronavirus disease 19 (COVID-19) with a hyperinflammatory state according to clinical and laboratory parameters. Methods: A cohort study was performed in 60 Spanish hospitals including 778 patients with COVID-19 and clinical and laboratory data indicative of a hyperinflammatory state. Treatment was mainly with tocilizumab, an intermediate-high dose of corticosteroids (IHDC), a pulse dose of corticosteroids (PDC), combination therapy, or no treatment. Primary outcome was intubation or death; follow-up was 21 days. Propensity score-adjusted estimations using Cox regression (logistic regression if needed) were calculated. Propensity scores were used as confounders, matching variables and for the inverse probability of treatment weights (IPTWs). Results: In all, 88, 117, 78 and 151 patients treated with tocilizumab, IHDC, PDC, and combination therapy, respectively, were compared with 344 untreated patients. The primary endpoint occurred in 10 (11.4%), 27 (23.1%), 12 (15.4%), 40 (25.6%) and 69 (21.1%), respectively. The IPTW-based hazard ratios (odds ratio for combination therapy) for the primary endpoint were 0.32 (95%CI 0.22-0.47; p < 0.001) for tocilizumab, 0.82 (0.71-1.30; p 0.82) for IHDC, 0.61 (0.43-0.86; p 0.006) for PDC, and 1.17 (0.86-1.58; p 0.30) for combination therapy. Other applications of the propensity score provided similar results, but were not significant for PDC. Tocilizumab was also associated with lower hazard of death alone in IPTW analysis (0.07; 0.02-0.17; p < 0.001). Conclusions: Tocilizumab might be useful in COVID-19 patients with a hyperinflammatory state and should be prioritized for randomized trials in this situatio

Impact of Biological Agents on Postsurgical Complications in Inflammatory Bowel Disease: A Multicentre Study of Geteccu

Background: The impact of biologics on the risk of postoperative complications (PC) in inflammatory bowel disease (IBD) is still an ongoing debate. This lack of evidence is more relevant for ustekinumab and vedolizumab. Aims: To evaluate the impact of biologics on the risk of PC. Methods: A retrospective study was performed in 37 centres. Patients treated with biologics within 12 weeks before surgery were considered “exposed”. The impact of the exposure on the risk of 30-day PC and the risk of infections was assessed by logistic regression and propensity score-matched analysis. Results: A total of 1535 surgeries were performed on 1370 patients. Of them, 711 surgeries were conducted in the exposed cohort (584 anti-TNF, 58 vedolizumab and 69 ustekinumab). In the multivariate analysis, male gender (OR: 1.5; 95% CI: 1.2–2.0), urgent surgery (OR: 1.6; 95% CI: 1.2–2.2), laparotomy approach (OR: 1.5; 95% CI: 1.1–1.9) and severe anaemia (OR: 1.8; 95% CI: 1.3–2.6) had higher risk of PC, while academic hospitals had significantly lower risk. Exposure to biologics (either anti-TNF, vedolizumab or ustekinumab) did not increase the risk of PC (OR: 1.2; 95% CI: 0.97–1.58), although it could be a risk factor for postoperative infections (OR 1.5; 95% CI: 1.03–2.27). Conclusions: Preoperative administration of biologics does not seem to be a risk factor for overall PC, although it may be so for postoperative infections

Twist exome capture allows for lower average sequence coverage in clinical exome sequencing

Background Exome and genome sequencing are the predominant techniques in the diagnosis and research of genetic disorders. Sufficient, uniform and reproducible/consistent sequence coverage is a main determinant for the sensitivity to detect single-nucleotide (SNVs) and copy number variants (CNVs). Here we compared the ability to obtain comprehensive exome coverage for recent exome capture kits and genome sequencing techniques. Results We compared three different widely used enrichment kits (Agilent SureSelect Human All Exon V5, Agilent SureSelect Human All Exon V7 and Twist Bioscience) as well as short-read and long-read WGS. We show that the Twist exome capture significantly improves complete coverage and coverage uniformity across coding regions compared to other exome capture kits. Twist performance is comparable to that of both short- and long-read whole genome sequencing. Additionally, we show that even at a reduced average coverage of 70× there is only minimal loss in sensitivity for SNV and CNV detection. Conclusion We conclude that exome sequencing with Twist represents a significant improvement and could be performed at lower sequence coverage compared to other exome capture techniques

A Solve-RD ClinVar-based reanalysis of 1522 index cases from ERN-ITHACA reveals common pitfalls and misinterpretations in exome sequencing

Purpose Within the Solve-RD project (https://solve-rd.eu/), the European Reference Network for Intellectual disability, TeleHealth, Autism and Congenital Anomalies aimed to investigate whether a reanalysis of exomes from unsolved cases based on ClinVar annotations could establish additional diagnoses. We present the results of the “ClinVar low-hanging fruit” reanalysis, reasons for the failure of previous analyses, and lessons learned. Methods Data from the first 3576 exomes (1522 probands and 2054 relatives) collected from European Reference Network for Intellectual disability, TeleHealth, Autism and Congenital Anomalies was reanalyzed by the Solve-RD consortium by evaluating for the presence of single-nucleotide variant, and small insertions and deletions already reported as (likely) pathogenic in ClinVar. Variants were filtered according to frequency, genotype, and mode of inheritance and reinterpreted. Results We identified causal variants in 59 cases (3.9%), 50 of them also raised by other approaches and 9 leading to new diagnoses, highlighting interpretation challenges: variants in genes not known to be involved in human disease at the time of the first analysis, misleading genotypes, or variants undetected by local pipelines (variants in off-target regions, low quality filters, low allelic balance, or high frequency). Conclusion The “ClinVar low-hanging fruit” analysis represents an effective, fast, and easy approach to recover causal variants from exome sequencing data, herewith contributing to the reduction of the diagnostic deadlock

Eines bioinformàtiques aplicades a l'estudi de l'espermatozoide i el fluid seminal

Aquest treball cerca per establir nous mètodes pel tractament de dades de proteòmica provinents de l'ús de TMT 10plex i cromatografia líquida seguida d'espectrometria de masses en tàndem (LC-MS/MS) i el seu estudi posterior, així com per l'estudi de RNAs i circRNAs d'espermatozoide provinents de la tecnologia d'alt rendiment, RNA-seq. En conclusió aquest treball s'aconsegueix crear un nou mètode per analitzar mostres de TMT seguides de LC-MS/MS amb problemes de quantificació i establir les bases per posar en marxa un punt de anàlisi bioinformàtic per a transcriptòmica.This work seeks to establish new methods for the analysis of proteomic data derived from the use of TMT 10plex and liquid chromatography followed by tandem mass spectrometry (LC-MS / MS) and its subsequent study, and for the study of RNA and circRNAs of sperm derived from RNA-seq technology. In conclusion, this work achieves the creation of a new method for analyzing TMT data followed by LC-MS / MS with quantification problems and establishing the bases to launch a bioinformatic analysis for RN-seq data.Este trabajo busca para establecer nuevos métodos por el tratamiento de datos de proteómica provenientes del uso de TMT 10plex y cromatografía líquida seguida de espectrometría de masas en tándem (LC-MS/MS) y su estudio posterior, así como por el estudio de RNAs y circRNAs de espermatozoide provenientes de la tecnología de alto rendimiento, RNA-seq. En conclusión este trabajo se consigue crear un nuevo método para analizar muestras de TMT seguidas de LC-MS/MS con problemas de cuantificación y establecer las bases para poner en marcha un punto de análisis bioinformàtic para transcriptòmica

Localización de atractores en sistemas caóticos y simulación de su dinámica basdo en esta misma

Teiss (Maestría en Ciencias en Sistemas Digitales), Instituto Politécnico Nacional, CITEDI, 2008, 1 archivo PDF, (79 páginas). tesis.ipn.m

Proteomic changes in human sperm during sequential in vitro capacitation and acrosome reaction

The male gamete is not completely mature after ejaculation and requires further events in the female genital tract to acquire fertilizing ability, including the processes of capacitation and acrosome reaction. In order to shed light on protein changes experienced by the sperm cell in preparation for fertilization, a comprehensive quantitative proteomic profiling based on isotopic peptide labeling and liquid chromatography followed by tandem mass spectrometry was performed on spermatozoa from three donors of proven fertility under three sequential conditions: purification with density gradient centrifugation, incubation with capacitation medium, and induction of acrosome reaction by exposure to the calcium ionophore A23187. After applying strict selection criteria for peptide quantification and for statistical analyses, 36 proteins with significant changes in their relative abundance within sperm protein extracts were detected. Moreover, the presence of peptide residues potentially harboring sites for post-translational modification was revealed, suggesting that protein modification may be an important mechanism in sperm maturation. In this regard, increased levels of proteins mainly involved in motility and signaling, both regulated by protein modifiers, were detected in sperm lysates following incubation with capacitation medium. In contrast, less abundant proteins in acrosome-reacted cell lysates did not contain potentially modifiable residues, suggesting the possibility that all those proteins might be relocated or released during the process. Protein-protein interaction analysis revealed a subset of proteins potentially involved in sperm maturation, including the proteins Erlin-2 (ERLIN2), Gamma-glutamyl hydrolase (GGH) and Transmembrane emp24 domain-containing protein 10 (TMED10). These results contribute to the current knowledge of the molecular basis of human fertilization. It should now be possible to further validate the potential role of the detected altered proteins as modulators of male infertility

La mirada y la comida: Un ejercicio de antropología visual acerca de prácticas alimenticias en Colombia

Hablamos entonces del comer como una experiencia que nos condiciona permanentemente como individuos, como sociedad,&nbsp; omo seres culturales y espirituales. El cocinar y el comer entendidos como conocimiento, herencia y sabiduría, pertenecientes a un grupo de personas, que hace parte de su cotidianidad, que se transfiere y que muta con el paso del tiempo, tiene un altísimo valor identificador, por lo que su culto y la continuidad de sus prácticas son elementales para la fijación de la identidad cultural y la memoria colectiva de cualquier grupo social, sea una familia, una comunidad, un pueblo, una región o un país