Elevated atmospheric concentrations of carbon dioxide reduce monarch tolerance and increase parasite virulence by altering the medicinal properties of milkweeds

Hosts combat their parasites using mechanisms of resistance and tolerance, which together determine parasite virulence. Environmental factors, including diet, mediate the impact of parasites on hosts, with diet providing nutritional and medicinal properties. Here, we present the first evidence that ongoing environmental change decreases host tolerance and increases parasite virulence through a loss of dietary medicinal quality. Monarch butterflies use dietary toxins (cardenolides) to reduce the deleterious impacts of a protozoan parasite. We fed monarch larvae foliage from four milkweed species grown under either elevated or ambient CO2, and measured changes in resistance, tolerance, and virulence. The most high‐cardenolide milkweed species lost its medicinal properties under elevated CO2; monarch tolerance to infection decreased, and parasite virulence increased. Declines in medicinal quality were associated with declines in foliar concentrations of lipophilic cardenolides. Our results emphasize that global environmental change may influence parasite–host interactions through changes in the medicinal properties of plants.Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/145415/1/ele13101-sup-0003-TableS1.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/145415/2/ele13101-sup-0007-TableS5.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/145415/3/ele13101-sup-0006-TableS4.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/145415/4/ele13101.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/145415/5/ele13101_am.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/145415/6/ele13101-sup-0004-TableS2.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/145415/7/ele13101-sup-0009-AppendixS1.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/145415/8/ele13101-sup-0005-TableS3.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/145415/9/ele13101-sup-0002-FigS2.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/145415/10/ele13101-sup-0008-TableS6.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/145415/11/ele13101-sup-0001-FigS1.pd

Phytochemical changes in milkweed induced by elevated CO2 alter wing morphology but not toxin sequestration in monarch butterflies

Environmental change has the potential to influence trophic interactions by altering the defensive phenotype of prey.Here, we examine the effects of a pervasive environmental change driver, elevated atmospheric concentrations of CO2 (eCO2), on toxin sequestration and flight morphology of a specialist herbivore.We fed monarch butterfly larvae, Danaus plexippus, foliage from four milkweed, Asclepias, species of varying chemical defence profiles grown under either ambient or eCO2. We also infected a subset of these herbivores with a protozoan parasite, Ophryocystis elektroscirrha, to understand how infection and environmental change combine to alter herbivore defences. We measured changes in phytochemistry induced by eCO2 and assessed cardenolide, toxic steroid, sequestration and wing morphology of butterflies.Monarchs compensated for lower plant cardenolide concentrations under eCO2 by increasing cardenolide sequestration rate, maintaining similar cardenolide composition and concentrations in their wings under both CO2 treatments. We suggest that these increases in sequestration rate are a by‐product of compensatory feeding aimed at maintaining a nutritional target in response to declining dietary quality under eCO2.Monarch wings were more suitable for sustained flight (more elongated) when reared on plants grown under eCO2 or when reared on Asclepias syriaca or Asclepias incarnata rather than on Asclepias curassavica or Asclepias speciosa. Parasite infection engendered wings less suitable for sustained flight (wings became rounder) on three of four milkweed species. Wing loading (associated with powered flight) was higher on A. syriaca than on other milkweeds, whereas wing density was lower on A. curassavica. Monarchs that fed on high cardenolide milkweed developed rounder, thinner wings, which are less efficient at gliding flight.Ingesting foliage from milkweed high in cardenolides may provide protection from enemies through sequestration yet come at a cost to monarchs manifested as lower quality flight phenotypes: rounder, thinner wings with lower wing loading values.Small changes in morphology may have important consequences for enemy evasion and migration success in many animals. Energetic costs associated with alterations in defence and morphology may, therefore, have important consequences for trophic interactions in a changing world.A plain language summary is available for this article.Plain Language SummaryPeer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/148239/1/fec13270-sup-0006-TableS2.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/148239/2/fec13270-sup-0003-FigS2.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/148239/3/fec13270-sup-0004-FigS3.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/148239/4/fec13270-sup-0002-FigS1.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/148239/5/fec13270-sup-0008-TableS4.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/148239/6/fec13270-sup-0005-TableS1.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/148239/7/fec13270-sup-0009-AppendixS1.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/148239/8/fec13270_am.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/148239/9/fec13270-sup-0001-Summary.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/148239/10/fec13270.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/148239/11/fec13270-sup-0007-TableS3.pd

Genetics of randomly bred cats support the cradle of cat domestication being in the Near East

Cat domestication likely initiated as a symbiotic relationship between wildcats (Felis silvestris subspecies) and the peoples of developing agrarian societies in the Fertile Crescent. As humans transitioned from hunter-gatherers to farmers ~12,000 years ago, bold wildcats likely capitalized on increased prey density (i.e., rodents). Humans benefited from the cats’ predation on these vermin. To refine the site(s) of cat domestication, over 1000 random-bred cats of primarily Eurasian descent were genotyped for single-nucleotide variants and short tandem repeats. The overall cat population structure suggested a single worldwide population with significant isolation by the distance of peripheral subpopulations. The cat population heterozygosity decreased as genetic distance from the proposed cat progenitor’s (F.s. lybica) natural habitat increased. Domestic cat origins are focused in the eastern Mediterranean Basin, spreading to nearby islands, and southernly via the Levantine coast into the Nile Valley. Cat population diversity supports the migration patterns of humans and other symbiotic species

Use of Motor Abundance in Young and Older Adults during Dual-Task Treadmill Walking

Contains fulltext : 110120.pdf (publisher's version ) (Open Access)Motor abundance allows individuals to perform any task reliably while being variable in movement's particulars. The study investigated age-related differences in this feature when young adults (YA) and older adults (OA) performed challenging tasks, namely treadmill walking alone and while performing a cognitive task. A goal function for treadmill walking was first defined, i.e., maintain constant speed at each step, which led to a goal equivalent manifold (GEM) containing all combinations of step time and step length that equally satisfied the function. Given the GEM, amounts of goal-equivalent and non-goal-equivalent variability were afterwards determined and used to define an index providing information about the set of effective motor solutions relative to the GEM. The set was limited in OA compared to YA in treadmill walking alone, indicating that OA made less flexible use of motor abundance than YA. However, this differentiation between YA and OA disappeared when concurrently performing the cognitive task. It is proposed that OA might have benefited from cognitive compensation

Applications and efficiencies of the first cat 63K DNA array

Correction, Volume: 8 Article Number: 8746 DOI: 10.1038/s41598-018-26885-5 Published: JUN 4 2018The development of high throughput SNP genotyping technologies has improved the genetic dissection of simple and complex traits in many species including cats. The properties of feline 62,897 SNPs Illumina Infinium iSelect DNA array are described using a dataset of over 2,000 feline samples, the most extensive to date, representing 41 cat breeds, a random bred population, and four wild felid species. Accuracy and efficiency of the array's genotypes and its utility in performing population-based analyses were evaluated. Average marker distance across the array was 37,741 Kb, and across the dataset, only 1% (625) of the markers exhibited poor genotyping and only 0.35% (221) showed Mendelian errors. Marker polymorphism varied across cat breeds and the average minor allele frequency (MAF) of all markers across domestic cats was 0.21. Population structure analysis confirmed a Western to Eastern structural continuum of cat breeds. Genome-wide linkage disequilibrium ranged from 50-1,500 Kb for domestic cats and 750 Kb for European wildcats (Felis silvestris silvestris). Array use in trait association mapping was investigated under different modes of inheritance, selection and population sizes. The efficient array design and cat genotype dataset continues to advance the understanding of cat breeds and will support monogenic health studies across feline breeds and populations.Peer reviewe

Cancer risks associated with germline PALB2 pathogenic variants: An international study of 524 families

PURPOSE To estimate age-specific relative and absolute cancer risks of breast cancer and to estimate risks of ovarian, pancreatic, male breast, prostate, and colorectal cancers associated with germline PALB2 pathogenic variants (PVs) because these risks have not been extensively characterized. METHODS We analyzed data from 524 families with PALB2 PVs from 21 countries. Complex segregation analysis was used to estimate relative risks (RRs; relative to country-specific population incidences) and absolute risks of cancers. The models allowed for residual familial aggregation of breast and ovarian cancer and were adjusted for the family-specific ascertainment schemes. RESULTS We found associations between PALB2 PVs and risk of female breast cancer (RR, 7.18; 95% CI, 5.82 to 8.85; P = 6.5 × 10-76), ovarian cancer (RR, 2.91; 95% CI, 1.40 to 6.04; P = 4.1 × 10-3), pancreatic cancer (RR, 2.37; 95% CI, 1.24 to 4.50; P = 8.7 × 10-3), and male breast cancer (RR, 7.34; 95% CI, 1.28 to 42.18; P = 2.6 3 1022). There was no evidence for increased risks of prostate or colorectal cancer. The breast cancer RRs declined with age (P for trend = 2.0 × 10-3). After adjusting for family ascertainment, breast cancer risk estimates on the basis of multiple case families were similar to the estimates from families ascertained through population-based studies (P for difference = .41). On the basis of the combined data, the estimated risks to age 80 years were 53% (95% CI, 44% to 63%) for female breast cancer, 5% (95% CI, 2% to 10%) for ovarian cancer, 2%-3% (95% CI females, 1% to 4%; 95% CI males, 2% to 5%) for pancreatic cancer, and 1% (95% CI, 0.2% to 5%) for male breast cancer. CONCLUSION These results confirm PALB2 as a major breast cancer susceptibility gene and establish substantial associations between germline PALB2 PVs and ovarian, pancreatic, and male breast cancers. These findings will facilitate incorporation of PALB2 into risk prediction models and optimize the clinical cancer risk management of PALB2 PV carriers

Data from: Elevated atmospheric concentrations of carbon dioxide reduce monarch tolerance and increase parasite virulence by altering the medicinal properties of milkweeds

Hosts combat their parasites using mechanisms of resistance and tolerance, which together determine parasite virulence. Environmental factors, including diet, mediate the impact of parasites on hosts, with diet providing nutritional and medicinal properties. Here, we present the first evidence that ongoing environmental change decreases host tolerance and increases parasite virulence through a loss of dietary medicinal quality. Monarch butterflies use dietary toxins (cardenolides) to reduce the deleterious impacts of a protozoan parasite. We fed monarch larvae foliage from four milkweed species grown under either elevated or ambient CO2, and measured changes in resistance, tolerance, and virulence. The most high-cardenolide milkweed species lost its medicinal properties under elevated CO2; monarch tolerance to infection decreased, and parasite virulence increased. Declines in medicinal quality were associated with declines in foliar concentrations of lipophilic cardenolides. Our results emphasize that global environmental change may influence parasite-host interactions through changes in the medicinal properties of plants

Elevated Atmospheric Concentrations of Carbon Dioxide Reduce Monarch Tolerance and Increase Parasite Virulence by Altering the Medicinal Properties of Milkweeds

This file contains two datasets describing monarch performance in response to parasitic infection in relation to changes in milkweed chemistry induced by elevated CO2

Data from: Phytochemical changes in milkweed induced by elevated CO2 alter wing morphology but not toxin sequestration in monarch butterflies

1. Environmental change has the potential to influence trophic interactions by altering the defensive phenotype of prey. 2. Here, we examine the effects of a pervasive environmental change driver, elevated atmospheric concentrations of CO2 (eCO2), on toxin sequestration and flight morphology of a specialist herbivore. 3. We fed monarch butterfly larvae, Danaus plexippus, foliage from four milkweed, Asclepias, species of varying chemical defense profiles grown under either ambient or eCO2. We also infected a subset of these herbivores with a protozoan parasite, Ophryocystis elektroscirrha, to understand how infection and environmental change combine to alter herbivore defenses. We measured changes in phytochemistry induced by eCO2 and assessed cardenolide, toxic steroid, sequestration and wing morphology of butterflies. 4. Monarchs compensated for lower plant cardenolide concentrations under eCO2 by increasing cardenolide sequestration rate, maintaining similar cardenolide composition and concentrations in their wings under both CO2 treatments. We suggest that these increases in sequestration rate are a byproduct of compensatory feeding aimed at maintaining a nutritional target in response to declining dietary quality. 5. Monarch wings were more suitable for sustained flight (more elongated) when reared on plants grown under eCO2 or when reared on A. syriaca or A. incarnata rather than on A. curassavica or A. speciosa. Parasite infection engendered wings less suitable for sustained flight (wings became rounder) on three of four milkweed species. Wing loading (associated with powered flight) was higher on A. syriaca than on other milkweeds, whereas wing density was lower on A. curassavica. Monarchs that fed on high cardenolide milkweed developed rounder, thinner wings, which are less efficient at gliding flight. 6. Ingesting foliage from milkweed high in cardenolides may provide protection from enemies through sequestration yet come at a cost to monarchs manifested as lower quality flight phenotypes: rounder, thinner wings with lower wing loading values. 7. Small changes in morphology may have important consequences for enemy evasion and migration success in many animals. Energetic costs associated with alterations in defense and morphology may, therefore, have important consequences for trophic interactions in a changing world