Minimal information for studies of extracellular vesicles (MISEV2023): From basic to advanced approaches

© 2024 The Authors. Journal of Extracellular Vesicles, published by Wiley Periodicals, LLC on behalf of the International Society for Extracellular Vesicles. This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY), https://creativecommons.org/licenses/by/4.0/Extracellular vesicles (EVs), through their complex cargo, can reflect the state of their cell of origin and change the functions and phenotypes of other cells. These features indicate strong biomarker and therapeutic potential and have generated broad interest, as evidenced by the steady year-on-year increase in the numbers of scientific publications about EVs. Important advances have been made in EV metrology and in understanding and applying EV biology. However, hurdles remain to realising the potential of EVs in domains ranging from basic biology to clinical applications due to challenges in EV nomenclature, separation from non-vesicular extracellular particles, characterisation and functional studies. To address the challenges and opportunities in this rapidly evolving field, the International Society for Extracellular Vesicles (ISEV) updates its 'Minimal Information for Studies of Extracellular Vesicles', which was first published in 2014 and then in 2018 as MISEV2014 and MISEV2018, respectively. The goal of the current document, MISEV2023, is to provide researchers with an updated snapshot of available approaches and their advantages and limitations for production, separation and characterisation of EVs from multiple sources, including cell culture, body fluids and solid tissues. In addition to presenting the latest state of the art in basic principles of EV research, this document also covers advanced techniques and approaches that are currently expanding the boundaries of the field. MISEV2023 also includes new sections on EV release and uptake and a brief discussion of in vivo approaches to study EVs. Compiling feedback from ISEV expert task forces and more than 1000 researchers, this document conveys the current state of EV research to facilitate robust scientific discoveries and move the field forward even more rapidly.Peer reviewe

Minimal information for studies of extracellular vesicles (MISEV2023): From basic to advanced approaches

Extracellular vesicles (EVs), through their complex cargo, can reflect the state of their cell of origin and change the functions and phenotypes of other cells. These features indicate strong biomarker and therapeutic potential and have generated broad interest, as evidenced by the steady year-on-year increase in the numbers of scientific publications about EVs. Important advances have been made in EV metrology and in understanding and applying EV biology. However, hurdles remain to realising the potential of EVs in domains ranging from basic biology to clinical applications due to challenges in EV nomenclature, separation from non-vesicular extracellular particles, characterisation and functional studies. To address the challenges and opportunities in this rapidly evolving field, the International Society for Extracellular Vesicles (ISEV) updates its 'Minimal Information for Studies of Extracellular Vesicles', which was first published in 2014 and then in 2018 as MISEV2014 and MISEV2018, respectively. The goal of the current document, MISEV2023, is to provide researchers with an updated snapshot of available approaches and their advantages and limitations for production, separation and characterisation of EVs from multiple sources, including cell culture, body fluids and solid tissues. In addition to presenting the latest state of the art in basic principles of EV research, this document also covers advanced techniques and approaches that are currently expanding the boundaries of the field. MISEV2023 also includes new sections on EV release and uptake and a brief discussion of in vivo approaches to study EVs. Compiling feedback from ISEV expert task forces and more than 1000 researchers, this document conveys the current state of EV research to facilitate robust scientific discoveries and move the field forward even more rapidly

Estudos sobre a população de Portunus spinicarpus (Stimpson, 1871) (Crustacea: Decapoda: Brachiura) da região do Cabo Frio, Brasil

During 33 months (January/86 to December/88) 99 trawlings have been made at 30, 45 and 60 m depth in front of Massambaba beach (Arraial do Cabo, R.J.), with lhe purpose to know the softbottom benthos of the in·fra littoral from an area characterized by the upwelling phenomenon. A Van-Veen grab, a rectangular dredge and an "otter-trawl" "' type net have been used. Portunus spinicarpus was the dominant species, summing up 85, 31. of all animals captured with the net. They are of small size (average size: CL= 5, 5 cm and W= 12, 03 g) and thus not comercialized in the region. Although captured in large amounts (120. 523 animals in 33 months) during daytime, it's peak of activity is nocturnal so the biomass of 630 g/20 min. trawls is underestimated. Males are larger (CL= 5, 65 cm) than females (CL= 5,35 cm) and the sex-ratio is 1, 1 m. :1 f.; however this relation changes according to depth, being males practically the double of females at 60 m. The egg-bearing females occur during the whole year, being their greatest numbers in Summer at 45 m and in Spring at 60 m. The smallest ones carrying eggs measured 3,9 cm (CL) and 3,65 g. Portunus spinicarpus population shows migratory movements apparently restricted to 45 and 60 m depth, which seems to be linked to food and temperature. The greatest abundance and benthic fauna diversity was observed at 45 m depth, whereas during Summer (and Spring, with intermitlent downwellings) this area remains watered by lhe cold upwelled waters from the ACAS (Central Waters of South Atlantic). It is discussed wheter this "migration" to 45 m would be dependent only on lower temperatures, or on the additional available food provided by the "trashfish" discarded from shrimp trawlers at this depth in Summer. The biological cycle of Portunus spinicarpus is performed in 12 months, with the development from egg to juveniles (CL= 3 cm) taking approximately 4 months. It is inferred from the obtained results that spawning occurs in Summer at 45 m depth, the larvae appear in Autumn, juvenile in Winter and pre-adults in Spring.Durante 33 meses (de Janeiro/86 a Dezembro/88) foram efetuados 99 arrastas diurnos nas profundidades de 30, 45 e 60 metros, em frente à praia de Massambaba (A. do Cabo, R.J.), com o objetivo de conhecer-se o bentos do infra-litoral de fundos moles, numa região caracterizada pelo fenômeno da ressurgência. Foram utilizados um pegador Van-Veen, uma draga retangular e uma rede de arrasto tipo "otter-trawl". Os siris Portunus spinicarpus constituíram-se na espécie dominante, totalizando 85,3% dos animais capturados com a rede. Esta espécie é de pequeno porte (tamanho médio: LC= 5,5 e P= 12,03 g) e, portanto, não comercializada na região. cm Embora tendo sido capturada em grandes quantidades nos arrastos diurnos (120. 523 animais durante o período de estudos) sua maior atividade é noturna, donde a biomassa de 630 g/arrasto de 20 minutos está subestimada. Os machos são maiores (LC= 5,65 cm) do que as fêmeas (LC= 5, 35 cm) e sua proporção é de 1, 1 m : 1 f ; entretanto esta relação modifica-se de acordo com as profundidades, sendo o praticamente o dobro do de fêmeas nos 60 metros. As fêmeas ovadas ocorrem durante todo o ano, seu número com a maior abundância no Verão a 45 metros e na Primavera a 60 metros, sendo que as menores ovadas capturadas mediam 3,9 cm (LC) e 3,65 g. A população de P. spinicarpus apresenta movimentos migratórios aparentemente restritos às profundidades de 45 e 60 metros, os quais parecem estar vinculados a alimentação e à temperatura. Na profundidade de 45 metros foi observada a maior abundância e diversidade de fauna bêntica, sendo que, durante o Verão (e na Primavera, com subsidências intermitentes) esta área permanece banhada pelas águas frias ressurgidas da ACAS (Água Central do Atlântico Sul); todavia discute-se se esta "migração" para os 45 metros seria dependente apenas das baixas temperaturas sobre a plataforma ou devido à maior disponibilidade de alimento adicional nesta profundidade, promovido pelo "by-catch" da pesca de camarões durante o Verão. O ciclo biológico dos Portunus spinicarpus na região estudada processa-se em 12 meses, com o desenvolvimento do ôvo a jovem (LC= 3 cm) em aproximadamente 4 meses. Infere-se dos resultados obtidos que as desovas ocorram no Verão a 45 metros, as larvas surjam no Outono, os jovens no Inverno e os pré-adultos na Primavera

Mémoire sur Miltiade et les auteurs de sa race

Séguier de Saint Brisson Nicolas Maximilien Sidoine. Mémoire sur Miltiade et les auteurs de sa race. In: Mémoires de l'Institut national de France, tome 15, 1ᵉ partie, 1842. pp. 137-214

Assessing the introduction of universal varicella vaccination in the Netherlands.

Although varicella is seen as a benign disease in the Netherlands, about 40,000 visits to a general practitioner (GP) are made, over 200 hospital admission occur, and 2.3 persons die on average each year. Most of this burden of disease can be prevented by universal varicella childhood vaccination. Ten years after the introduction of the single-shot, single-component varicella childhood vaccination in the USA, a major reduction in hospitalization, mortality, and burden of disease has been reported. Using our recently vaccine evaluation model for the introduction of a new vaccine in our national immunization program, we have analyzed the feasibility of universal varicella vaccination by replacing the measles-mumps-rubella (MMR) vaccine with a measles-mumps-rubella-varicella (MMRV) vaccine. After structuring and reviewing the available data, two major points of uncertainty remain: (1) the influence of universal childhood vaccination on the incidence of zoster later in life; (2) the cost-effectiveness ratio for the Dutch situation. Despite these uncertainties it is clear that universal childhood vaccination will prevent most of the varicella related GP-visits, hospitalizations, and deaths

« Numénius et la tradition judéo-hellénistique : une relecture du fragment 21 F (13 dP) », Semitica et classica, 2021 (sous presse).

International audienc

La bataille de l'imprimé

Il est difficile d'exagérer l'ampleur de la révolution que nous sommes en train de vivre et la tourmente dans laquelle est entraîné inexorablement le livre. Celui-ci n'est déjà plus le vecteur du savoir qu'il a été depuis plus de cinq millénaires. Le bouleversement dont nous sommes témoins sera plus radical que l'invention de l'imprimerie et de plus longue portée que le passage du volumen (ou rouleau de papyrus) au codex (ou cahier cousu et ancêtre de notre livre).Pour Sandrine et Hélène Arwe