Plasticity of the Berry Ripening Program in a White Grape Variety

Grapevine (Vitis vinifera L) is considered one of the most environmentally sensitive crops and is characterized by broad phenotypic plasticity, offering important advantages such as the large range of different wines that can be produced from the same cultivar, and the adaptation of existing cultivars to diverse growing regions. The uniqueness of berry quality traits reflects complex interactions between the grapevine plant and the combination of natural factors and human cultural practices, defined as terroir, which leads to the expression of wine typicity. Despite the scientific and commercial importance of genotype interactions with growing conditions, few studies have characterized the genes and metabolites directly involved in this phenomenon. Here we used two large-scale analytical approaches to explore the metabolomic and transcriptomic basis of the broad phenotypic plasticity of Garganega, a white berry variety grown at four sites characterized by different pedoclimatic conditions (altitudes, soil texture and composition). These conditions determine berry ripening dynamics in terms of sugar accumulation and the abundance of phenolic compounds. Multivariate analysis unraveled a highly plastic metabolomic response to different environments, especially the accumulation of hydroxycinnamic and hydroxybenzoic acids and flavonols. Principal component analysis revealed that the four sites strongly affected the berry transcriptome allowing the identification of environmentally-modulated genes and the plasticity of commonly-modulated transcripts at different sites. Many genes that control transcription, translation, transport and carbohydrate metabolism showed different expression depending on the environmental conditions, indicating a key role in the observed transcriptomic plasticity of Garganega berries. Interestingly, genes representing the phenylpropanoid/flavonoid pathway showed plastic responses to the environment mirroring the accumulation of the corresponding metabolites. The comparison of Garganega and Corvina berries showed that the metabolism of phenolic compounds is more plastic in ripening Garganega berries under different pedoclimatic conditions

The deceleration of Giant Herbig-Haro Flows

It has been recently discovered that spatially separated Herbig-Haro objects, once considered unrelated, are linked within a chain that may extend for parsecs in either direction of the embedded protostar forming a "giant Herbig-Haro jet". Presently, several dozen of these giant flows have been detected and the best documented example, the HH~34 system, shows a systematic velocity decrease with distance on either side of the source. In this paper, we have modeled giant jets by performing fully three-dimensional simulations of overdense, radiatively cooling jets modulated with long-period (P $\sim$ several hundred years) and large amplitude sinusoidal velocity variability at injection ($\Delta v \sim$ mean jet flow velocity). Allowing them to travel over a distance well beyond the source, we have found that multiple travelling pulses develop and their velocity indeed falls off smoothly and systematically with distance. This deceleration is fastest if the jet is pressure-confined, in which case the falloff in velocity is roughly consistent with the observations. The deceleration occurs as momentum is transferred by gas expelled sideways from the traveling pulses. The simulation of a pressure-confined, steady-state jet with similar initial conditions to those of the pulsed jet shows that the flow in this case experiences $acceleration$. This result is thus an additional indication that the primary source of deceleration in the giant flows $cannot$ be attributed to braking of the jet head against the external medium.Comment: 25 pages, 10 figures (see higher resolution figures in: http://www.iagusp.usp.br/~dalpino/giants/apj00.tar.gz); ApJ in pres

A Gamma-convergence argument for the blow-up of a non-local semilinear parabolic equation with Neumann boundary conditions

In this paper we study a simple non-local semilinear parabolic equation with Neumann boundary condition. We give local existence result and prove global existence for small initial data. A natural non increasing in time energy is associated to this equation. We prove that the solution blows up at finite time $T$ if and only if its energy is negative at some time before $T$. The proof of this result is based on a Gamma-convergence technique

Milky Way Disk-Halo Transition in HI: Properties of the Cloud Population

Using 21cm HI observations from the Parkes Radio Telescope's Galactic All-Sky Survey, we measure 255 HI clouds in the lower Galactic halo that are located near the tangent points at 16.9 < l < 35.3 degrees and |b| < 20 degrees. The clouds have a median mass of 700 Msun and a median distance from the Galactic plane of 660 pc. This first Galactic quadrant (QI) region is symmetric to a region of the fourth quadrant (QIV) studied previously using the same data set and measurement criteria. The properties of the individual clouds in the two quadrants are quite similar suggesting that they belong to the same population, and both populations have a line of sight cloud-cloud velocity dispersion of sigma_cc ~ 16 km/s. However, there are three times as many disk-halo clouds at the QI tangent points and their scale height, at h=800 pc, is twice as large as in QIV. Thus the observed line of sight random cloud motions are not connected to the cloud scale height or its variation around the Galaxy. The surface density of clouds is nearly constant over the QI tangent point region but is peaked near R~4 kpc in QIV. We ascribe all of these differences to the coincidental location of the QI region at the tip of the Milky Way's bar, where it merges with a major spiral arm. The QIV tangent point region, in contrast, covers only a segment of a minor spiral arm. The disk-halo HI cloud population is thus likely tied to and driven by large-scale star formation processes, possibly through the mechanism of supershells and feedback.Comment: 14 pages, 20 figures, to be published in ApJ (accepted August 3 2010

Patterns of variability in Be/X-ray pulsars during giant outbursts

The discovery of source states in the X-ray emission of black-hole binaries and neutron-star low-mass X-ray binaries constituted a major step forward in the understanding of the physics of accretion onto compact objects. While there are numerous studies on the correlated timing and spectral variability of these systems, very little work has been done on high-mass X-ray binaries, the third major type of X-ray binaries. The main goal of this work is to investigate whether Be accreting X-ray pulsars display source states and characterise those states through their spectral and timing properties. We have made a systematic study of the power spectra, energy spectra and X-ray hardness-intensity diagrams of nine Be/X-ray pulsars. The evolution of the timing and spectral parameters were monitored through changes over two orders of magnitude in luminosity. We find that Be/X-ray pulsars trace two different branches in the hardness-intensity diagram: the horizontal branch corresponds to a low-intensity state of the source and it is characterised by fast colour and spectral changes and high X-ray variability. The diagonal branch is a high-intensity state that emerges when the X-ray luminosity exceeds a critical limit. The photon index anticorrelates with X-ray flux in the horizontal branch but correlates with it in the diagonal branch. The correlation between QPO frequency and X-ray flux reported in some pulsars is also observed if the peak frequency of the broad-band noise that accounts for the aperiodic variability is used. The two branches may reflect two different accretion modes, depending on whether the luminosity of the source is above or below a critical value. This critical luminosity is mainly determined by the magnetic field strength, hence it differs for different sources.Comment: Complete missing words in title. Proof corrections adde

Increasing thyroid cancer incidence in Lithuania in 1978–2003

BACKGROUND: The aim of this paper is to analyze changes in thyroid cancer incidence trends in Lithuania during the period 1978–2003 using joinpoint regression models, with special attention to the period 1993–2003. METHODS: The study was based on all cases of thyroid cancer reported to the Lithuanian Cancer Registry between 1978 and 2003. Age group-specific rates and standardized rates were calculated for each gender, using the direct method (world standard population). The joinpoint regression model was used to provide estimated annual percentage change and to detect points in time where significant changes in the trends occur. RESULTS: During the study period the age-standardized incidence rates increased in males from 0.7 to 2.5 cases per 100 000 and in females from 1.5 to 11.4 per 100 000. Annual percentage changes during this period in the age-standardized rates were 4.6% and 7.1% for males and females, respectively. Joinpoint analysis showed two time periods with joinpoint in the year 2000. A change in the trend occurred in which a significant increase changed to a dramatic increase in thyroid cancer incidence rates. Papillary carcinoma and stage I thyroid cancer increases over this period were mainly responsible for the pattern of changes in trend in recent years. CONCLUSION: A moderate increase in thyroid cancer incidence has been observed in Lithuania between the years 1978 and 2000. An accelerated increase in thyroid cancer incidence rates took place in the period 2000–2003. It seems that the increase in thyroid cancer incidence can be attributed mainly to the changes in the management of non palpable thyroid nodules with growing applications of ultrasound-guided fine needle aspiration biopsy in clinical practice

Explicit vs. tacit collusion: the impact of communication in oligopoly experiments

publication-status: Acceptedtypes: ArticlePre-print draft published as working paper by Düsseldorf Institute for Competition Economics (DICE). NOTICE: this is the author’s version of a work that was accepted for publication in European Economic Review. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in European Economic Review, Elsevier, vol. 56(8), 2012, DOI: 10.1016/j.euroecorev.2012.09.002We explore the difference between explicit and tacit collusion by investigating the impact communication has in experimental markets. For Bertrand oligopolies with various numbers of firms, we compare pricing behavior with and without the possibility to communicate among firms. We find strong evidence that talking helps to obtain higher pro fits for any number of firms, however, the gain from communicating is nonmonotonic in the number of firms, with medium-sized industries having the largest additional profi t from talking. We also find that industries continue to collude successfully after communication is disabled. Communication supports firms in coordinating on collusive pricing schemes, and it is also used for conflict mediation.Financial support from Deutsche Forschungsgemeinschaft (DFG) is gratefully acknowledge

International variation in ethics committee requirements: comparisons across five Westernised nations

BACKGROUND: Ethics committees typically apply the common principles of autonomy, nonmaleficence, beneficence and justice to research proposals but with variable weighting and interpretation. This paper reports a comparison of ethical requirements in an international cross-cultural study and discusses their implications. DISCUSSION: The study was run concurrently in New Zealand, UK, Israel, Canada and USA and involved testing hypotheses about believability of testimonies regarding alleged child sexual abuse. Ethics committee requirements to conduct this study ranged from nil in Israel to considerable amendments designed to minimise participant harm in New Zealand. Assessment of minimal risk is a complex and unreliable estimation further compounded by insufficient information on probabilities of particular individuals suffering harm. Estimating potential benefits/ risks ratio and protecting participants' autonomy similarly are not straightforward exercises. SUMMARY: Safeguarding moral/humane principles should be balanced with promotion of ethical research which does not impede research posing minimal risk to participants. In ensuring that ethical standards are met and research has scientific merit, ethics committees have obligations to participants (to meet their rights and protect them from harm); to society (to ensure good quality research is conducted); and to researchers (to treat their proposals with just consideration and respect). To facilitate meeting all these obligations, the preferable focus should be promotion of ethical research, rather than the prevention of unethical research, which inevitably results in the impediment of researchers from doing their work. How the ethical principles should be applied and balanced requires further consideration

Forcing single-column models using high-resolution model simulations

To use single column models (SCMs) as a research tool for parametrisation development and process studies, the SCM must be supplied with realistic initial profiles, forcing fields and boundary conditions. We propose a new technique for deriving these required profiles, motivated by the increase in number and scale of high-resolution convection-permitting simulations. We suggest that these high-resolution simulations be coarse-grained to the required resolution of an SCM, and thereby be used as a proxy for the ‘true’ atmosphere. This paper describes the implementation of such a technique. We test the proposed methodology using high-resolution data from the UK Met Office’s Unified Model (MetUM), with a resolution of 4 km, covering a large tropical domain. This data is coarse grained and used to drive the European Centre for Medium-Range Weather Forecast’s (ECMWF) Integrated Forecasting 26 System (IFS) SCM. The proposed method is evaluated by deriving IFS SCM forcing profiles from a consistent T639 IFS simulation. The SCM simulations track the global model, indicating a consistency between the estimated forcing fields and the ‘true’ dynamical forcing in the global model. We demonstrate the benefits of selecting SCM forcing profiles from across a large-domain, namely robust statistics, and the ability to test the SCM over a range of boundary conditions. We also compare driving the SCM with the coarse-grained datase to driving it using the ECMWF operational analysis. We conclude by highlighting the importance of understanding biases in the high-resolution dataset, and suggest that our approach be used in combination with observationally derived forcing datasets