Snakes and snake bite in Nepal

At Nepal's northern border with Tibet is the great Himalaya Range. capped by Mount Everest itself (8848 ni) and including four of the other eight highest mountains in the world. South of the Kathmandu Valley at the centre of the country are the lesser mountains of the Mahabharat Range and Churia Ghati Hills. below 3000 m, and in the far south, stretching to India. are the fertile alluvial Terai Plains with scattered swamps and hardwood and bamboo forests. The distribution of the 14 species of venomous snakes known to inhabit Nepal is determined by geography and climate. The range of only one species. the Himalayan pit viper (Agkistrotlori hir~itilri~tiiiu.s), extends into the higher elevations.Published versio

Quality of cookies with partial substitution of wheat flour for okra flour

Okra is a vegetable of high nutritional value, but highly perishable when stored in natura. Cookies are products of great popular acceptance and allow the use of alternative flours. The objective was to evaluate cookies made with partial substitution of wheat flour for okra. Okra fruits were dried to obtain the flour. This was applied in biscuit formulations at concentrations of 0 (A), 5 (B), 10 (C) and 15% (D). The cookies were evaluated for the difference in mass, thermal factor, yield, humidity, ash, fibers, proteins, lipids, carbohydrates, caloric value, presence of Coliforms at 45 ° C, positive coagulase Staphylococcus and Samonella sp., In addition to the sensory evaluation acceptance and purchase intention. The dough, ash content, fiber and protein of the cookies enriched with okra flour were higher. The carbohydrate content and caloric value was higher in formulation A. All cookies are within the microbiological standards required by law. Sensory acceptance and purchase intention were superior in formulations A and B. The differences between the cookies are due to the different composition of the okra and vary according to the concentration in which it is added. Formulation B obtained better nutritional quality without interfering with its good sensory acceptance.Okra is a vegetable of high nutritional value, but highly perishable when stored in natura. Cookies are products of great popular acceptance and allow the use of alternative flours. The objective was to evaluate cookies made with partial substitution of wheat flour for okra. Okra fruits were dried to obtain the flour. This was applied in biscuit formulations at concentrations of 0 (A), 5 (B), 10 (C) and 15% (D). The cookies were evaluated for the difference in mass, thermal factor, yield, humidity, ash, fibers, proteins, lipids, carbohydrates, caloric value, presence of Coliforms at 45 ° C, positive coagulase Staphylococcus and Samonella sp., In addition to the sensory evaluation acceptance and purchase intention. The dough, ash content, fiber and protein of the cookies enriched with okra flour were higher. The carbohydrate content and caloric value was higher in formulation A. All cookies are within the microbiological standards required by law. Sensory acceptance and purchase intention were superior in formulations A and B. The differences between the cookies are due to the different composition of the okra and vary according to the concentration in which it is added. Formulation B obtained better nutritional quality without interfering with its good sensory acceptance

Symplectic and Poisson geometry on b-manifolds

Let $M^{2n}$ be a Poisson manifold with Poisson bivector field $\Pi$. We say that $M$ is b-Poisson if the map $\Pi^n:M\to\Lambda^{2n}(TM)$ intersects the zero section transversally on a codimension one submanifold $Z\subset M$. This paper will be a systematic investigation of such Poisson manifolds. In particular, we will study in detail the structure of $(M,\Pi)$ in the neighbourhood of $Z$ and using symplectic techniques define topological invariants which determine the structure up to isomorphism. We also investigate a variant of de Rham theory for these manifolds and its connection with Poisson cohomology.Comment: 34 pages. Some changes have been implemented mainly in Sections 2 and 6. Minor changes in exposition. References have been adde

Vanishing Viscous Limits for 3D Navier-Stokes Equations with A Navier-Slip Boundary Condition

In this paper, we investigate the vanishing viscosity limit for solutions to the Navier-Stokes equations with a Navier slip boundary condition on general compact and smooth domains in $\mathbf{R}^3$. We first obtain the higher order regularity estimates for the solutions to Prandtl's equation boundary layers. Furthermore, we prove that the strong solution to Navier-Stokes equations converges to the Eulerian one in $C([0,T];H^1(\Omega))$ and L^\infty((0,T)\times\o), where $T$ is independent of the viscosity, provided that initial velocity is regular enough. Furthermore, rates of convergence are obtained also.Comment: 45page

A bovine lymphosarcoma cell line infected with theileria annulata exhibits an irreversible reconfiguration of host cell gene expression

Theileria annulata, an intracellular parasite of bovine lymphoid cells, induces substantial phenotypic alterations to its host cell including continuous proliferation, cytoskeletal changes and resistance to apoptosis. While parasite induced modulation of host cell signal transduction pathways and NFκB activation are established, there remains considerable speculation on the complexities of the parasite directed control mechanisms that govern these radical changes to the host cell. Our objectives in this study were to provide a comprehensive analysis of the global changes to host cell gene expression with emphasis on those that result from direct intervention by the parasite. By using comparative microarray analysis of an uninfected bovine cell line and its Theileria infected counterpart, in conjunction with use of the specific parasitacidal agent, buparvaquone, we have identified a large number of host cell gene expression changes that result from parasite infection. Our results indicate that the viable parasite can irreversibly modify the transformed phenotype of a bovine cell line. Fifty percent of genes with altered expression failed to show a reversible response to parasite death, a possible contributing factor to initiation of host cell apoptosis. The genes that did show an early predicted response to loss of parasite viability highlighted a sub-group of genes that are likely to be under direct control by parasite infection. Network and pathway analysis demonstrated that this sub-group is significantly enriched for genes involved in regulation of chromatin modification and gene expression. The results provide evidence that the Theileria parasite has the regulatory capacity to generate widespread change to host cell gene expression in a complex and largely irreversible manner

Expression Analysis of the Theileria parva Subtelomere-Encoded Variable Secreted Protein Gene Family

Background The intracellular protozoan parasite Theileria parva transforms bovine lymphocytes inducing uncontrolled proliferation. Proteins released from the parasite are assumed to contribute to phenotypic changes of the host cell and parasite persistence. With 85 members, genes encoding subtelomeric variable secreted proteins (SVSPs) form the largest gene family in T. parva. The majority of SVSPs contain predicted signal peptides, suggesting secretion into the host cell cytoplasm. Methodology/Principal Findings We analysed SVSP expression in T. parva-transformed cell lines established in vitro by infection of T or B lymphocytes with cloned T. parva parasites. Microarray and quantitative real-time PCR analysis revealed mRNA expression for a wide range of SVSP genes. The pattern of mRNA expression was largely defined by the parasite genotype and not by host background or cell type, and found to be relatively stable in vitro over a period of two months. Interestingly, immunofluorescence analysis carried out on cell lines established from a cloned parasite showed that expression of a single SVSP encoded by TP03_0882 is limited to only a small percentage of parasites. Epitope-tagged TP03_0882 expressed in mammalian cells was found to translocate into the nucleus, a process that could be attributed to two different nuclear localisation signals. Conclusions Our analysis reveals a complex pattern of Theileria SVSP mRNA expression, which depends on the parasite genotype. Whereas in cell lines established from a cloned parasite transcripts can be found corresponding to a wide range of SVSP genes, only a minority of parasites appear to express a particular SVSP protein. The fact that a number of SVSPs contain functional nuclear localisation signals suggests that proteins released from the parasite could contribute to phenotypic changes of the host cell. This initial characterisation will facilitate future studies on the regulation of SVSP gene expression and the potential biological role of these enigmatic proteins

First observations of separated atmospheric nu_mu and bar{nu-mu} events in the MINOS detector

The complete 5.4 kton MINOS far detector has been taking data since the beginning of August 2003 at a depth of 2070 meters water-equivalent in the Soudan mine, Minnesota. This paper presents the first MINOS observations of nuµ and [overline nu ]µ charged-current atmospheric neutrino interactions based on an exposure of 418 days. The ratio of upward- to downward-going events in the data is compared to the Monte Carlo expectation in the absence of neutrino oscillations, giving Rup/downdata/Rup/downMC=0.62-0.14+0.19(stat.)±0.02(sys.). An extended maximum likelihood analysis of the observed L/E distributions excludes the null hypothesis of no neutrino oscillations at the 98% confidence level. Using the curvature of the observed muons in the 1.3 T MINOS magnetic field nuµ and [overline nu ]µ interactions are separated. The ratio of [overline nu ]µ to nuµ events in the data is compared to the Monte Carlo expectation assuming neutrinos and antineutrinos oscillate in the same manner, giving R[overline nu ][sub mu]/nu[sub mu]data/R[overline nu ][sub mu]/nu[sub mu]MC=0.96-0.27+0.38(stat.)±0.15(sys.), where the errors are the statistical and systematic uncertainties. Although the statistics are limited, this is the first direct observation of atmospheric neutrino interactions separately for nuµ and [overline nu ]µ

A Functional Gene Array for Detection of Bacterial Virulence Elements

Emerging known and unknown pathogens create profound threats to public health. Platforms for rapid detection and characterization of microbial agents are critically needed to prevent and respond to disease outbreaks. Available detection technologies cannot provide broad functional information about known or novel organisms. As a step toward developing such a system, we have produced and tested a series of high-density functional gene arrays to detect elements of virulence and antibiotic resistance mechanisms. Our first generation array targets genes from Escherichia coli strains K12 and CFT073, Enterococcus faecalis and Staphylococcus aureus. We determined optimal probe design parameters for gene family detection and discrimination. When tested with organisms at varying phylogenetic distances from the four target strains, the array detected orthologs for the majority of targeted gene families present in bacteria belonging to the same taxonomic family. In combination with whole-genome amplification, the array detects femtogram concentrations of purified DNA, either spiked in to an aerosol sample background, or in combinations from one or more of the four target organisms. This is the first report of a high density NimbleGen microarray system targeting microbial antibiotic resistance and virulence mechanisms. By targeting virulence gene families as well as genes unique to specific biothreat agents, these arrays will provide important data about the pathogenic potential and drug resistance profiles of unknown organisms in environmental samples