300 research outputs found
Barrier and internal wave contributions to the quantum probability density and flux in light heavy-ion elastic scattering
We investigate the properties of the optical model wave function for light
heavy-ion systems where absorption is incomplete, such as Ca
and O around 30 MeV incident energy. Strong focusing effects
are predicted to occur well inside the nucleus, where the probability density
can reach values much higher than that of the incident wave. This focusing is
shown to be correlated with the presence at back angles of a strong enhancement
in the elastic cross section, the so-called ALAS (anomalous large angle
scattering) phenomenon; this is substantiated by calculations of the quantum
probability flux and of classical trajectories. To clarify this mechanism, we
decompose the scattering wave function and the associated probability flux into
their barrier and internal wave contributions within a fully quantal
calculation. Finally, a calculation of the divergence of the quantum flux shows
that when absorption is incomplete, the focal region gives a sizeable
contribution to nonelastic processes.Comment: 16 pages, 15 figures. RevTeX file. To appear in Phys. Rev. C. The
figures are only available via anonynous FTP on
ftp://umhsp02.umh.ac.be/pub/ftp_pnt/figscat
Nonlinear Elasticity in Biological Gels
Unlike most synthetic materials, biological materials often stiffen as they
are deformed. This nonlinear elastic response, critical for the physiological
function of some tissues, has been documented since at least the 19th century,
but the molecular structure and the design principles responsible for it are
unknown. Current models for this response require geometrically complex ordered
structures unique to each material. In this Article we show that a much simpler
molecular theory accounts for strain stiffening in a wide range of molecularly
distinct biopolymer gels formed from purified cytoskeletal and extracellular
proteins. This theory shows that systems of semi-flexible chains such as
filamentous proteins arranged in an open crosslinked meshwork invariably
stiffen at low strains without the need for a specific architecture or multiple
elements with different intrinsic stiffnesses.Comment: 23 pages, 5 figures, submitted to Natur
Strain-controlled criticality governs the nonlinear mechanics of fibre networks
Disordered fibrous networks are ubiquitous in nature as major structural
components of living cells and tissues. The mechanical stability of networks
generally depends on the degree of connectivity: only when the average number
of connections between nodes exceeds the isostatic threshold are networks
stable (Maxwell, J. C., Philosophical Magazine 27, 294 (1864)). Upon increasing
the connectivity through this point, such networks undergo a mechanical phase
transition from a floppy to a rigid phase. However, even sub-isostatic networks
become rigid when subjected to sufficiently large deformations. To study this
strain-controlled transition, we perform a combination of computational
modeling of fibre networks and experiments on networks of type I collagen
fibers, which are crucial for the integrity of biological tissues. We show
theoretically that the development of rigidity is characterized by a
strain-controlled continuous phase transition with signatures of criticality.
Our experiments demonstrate mechanical properties consistent with our model,
including the predicted critical exponents. We show that the nonlinear
mechanics of collagen networks can be quantitatively captured by the
predictions of scaling theory for the strain-controlled critical behavior over
a wide range of network concentrations and strains up to failure of the
material
ANIA:ANnotation and Integrated Analysis of the 14-3-3 interactome
The dimeric 14-3-3 proteins dock onto pairs of phosphorylated Ser and Thr residues on hundreds of proteins, and thereby regulate many events in mammalian cells. To facilitate global analyses of these interactions, we developed a web resource named ANIA: ANnotation and Integrated Analysis of the 14-3-3 interactome, which integrates multiple data sets on 14-3-3-binding phosphoproteins. ANIA also pinpoints candidate 14-3-3-binding phosphosites using predictor algorithms, assisted by our recent discovery that the human 14-3-3-interactome is highly enriched in 2R-ohnologues. 2R-ohnologues are proteins in families of two to four, generated by two rounds of whole genome duplication at the origin of the vertebrate animals. ANIA identifies candidate ‘lynchpins’, which are 14-3-3-binding phosphosites that are conserved across members of a given 2R-ohnologue protein family. Other features of ANIA include a link to the catalogue of somatic mutations in cancer database to find cancer polymorphisms that map to 14-3-3-binding phosphosites, which would be expected to interfere with 14-3-3 interactions. We used ANIA to map known and candidate 14-3-3-binding enzymes within the 2R-ohnologue complement of the human kinome. Our projections indicate that 14-3-3s dock onto many more human kinases than has been realized. Guided by ANIA, PAK4, 6 and 7 (p21-activated kinases 4, 6 and 7) were experimentally validated as a 2R-ohnologue family of 14-3-3-binding phosphoproteins. PAK4 binding to 14-3-3 is stimulated by phorbol ester, and involves the ‘lynchpin’ site phosphoSer99 and a major contribution from Ser181. In contrast, PAK6 and PAK7 display strong phorbol ester-independent binding to 14-3-3, with Ser113 critical for the interaction with PAK6. These data point to differential 14-3-3 regulation of PAKs in control of cell morphology. Database URL: https://ania-1433.lifesci.dundee.ac.uk/prediction/webserver/index.p
Mating system of the Eurasian badger, Meles meles, in a high density population
Badgers are facultatively social, forming large groups at high density. Group-living appears
to have high reproductive costs for females, and may lead to increased levels of inbreeding.
The extent of female competition for reproduction has been estimated from field data, but
knowledge of male reproductive success and the extent of extra-group paternity remains
limited. Combining field data with genetic data (16 microsatellite loci), we studied the mating
system of 10 badger social groups across 14 years in a high-density population. From 923
badgers, including 425 cubs, we were able to assign maternity to 307 cubs, with both parents
assigned to 199 cubs (47%) with 80% confidence, and 14% with 95% confidence. Age had a
significant effect on the probability of reproduction, seemingly as a result of a deficit of
individuals aged two years and greater than eight years attaining parentage. We estimate
that approximately 30% of the female population successfully reproduced in any given
year, with a similar proportion of the male population gaining paternity across the same
area. While it was known there was a cost to female reproduction in high density populations,
it appears that males suffer similar, but not greater, costs. Roughly half of assigned paternity
was attributed to extra-group males, the majority of which were from neighbouring social
groups. Few successful matings occurred between individuals born in the same social group
(22%). The high rate of extra-group mating, previously unquantified, may help reduce inbreeding,
potentially making philopatry a less costly strategy
Rethinking health sector procurement as developmental linkages in East Africa
Health care forms a large economic sector in all countries, and procurement of medicines and other essential commodities necessarily creates economic linkages between a country's health sector and local and international industrial development. These procurement processes may be positive or negative in their effects on populations' access to appropriate treatment and on local industrial development, yet procurement in low and middle income countries (LMICs) remains under-studied: generally analysed, when addressed at all, as a public sector technical and organisational challenge rather than a social and economic element of health system governance shaping its links to the wider economy. This article uses fieldwork in Tanzania and Kenya in 2012–15 to analyse procurement of essential medicines and supplies as a governance process for the health system and its industrial links, drawing on aspects of global value chain theory. We describe procurement work processes as experienced by front line staff in public, faith-based and private sectors, linking these experiences to wholesale funding sources and purchasing practices, and examining their implications for medicines access and for local industrial development within these East African countries. We show that in a context of poor access to reliable medicines,
extensive reliance on private medicines purchase, and increasing globalisation of procurement systems, domestic linkages between health and industrial sectors have been weakened, especially in Tanzania. We argue in consequence for a more developmental perspective on health sector procurement design, including closer policy attention to strengthening vertical and horizontal relational working within local health-industry value chains, in the interests of both wider access to treatment and improved industrial development in Africa
Single-crosslink microscopy in a biopolymer network dissects local elasticity from molecular fluctuations
Polymer networks are fundamental from cellular biology to plastics technology but their intrinsic inhomogeneity is masked by the usual ensemble-averaged measurements. Here, we construct direct maps of crosslinks-symbolic depiction of spatially-distributed elements highlighting their physical features and the relationships between them-in an actin network. We selectively label crosslinks with fluorescent markers, track their thermal fluctuations, and characterize the local elasticity and cross-correlations between crosslinks. Such maps display massive heterogeneity, reveal abundant anticorrelations, and may contribute to address how local responses scale up to produce macroscopic elasticity. Single-crosslink microscopy offers a general, microscopic framework to better understand crosslinked molecular networks in undeformed or strained states
Using formative research to develop the healthy eating component of the CHANGE! school-based curriculum intervention
Background: Childhood obesity is a significant public health concern. Many intervention studies have attempted
to combat childhood obesity, often in the absence of formative or preparatory work. This study describes the
healthy eating component of the formative phase of the Children’s Health Activity and Nutrition: Get Educated!
(CHANGE!) project. The aim of the present study was to gather qualitative focus group and interview data
regarding healthy eating particularly in relation to enabling and influencing factors, barriers and knowledge in
children and adults (parents and teachers) from schools within the CHANGE! programme to provide populationspecific
evidence to inform the subsequent intervention design.
Methods: Semi-structured focus group interviews were conducted with children, parents and teachers across 11
primary schools in the Wigan borough of North West England. Sixty children (N = 24 boys), 33 parents (N = 4 male)
and 10 teachers (N = 4 male) participated in the study. Interview questions were structured around the PRECEDE
phases of the PRECEDE-PROCEED model. Interviews were transcribed verbatim and analysed using the pen-profiling
technique.
Results: The pen-profiles revealed that children’s knowledge of healthy eating was generally good, specifically
many children were aware that fruit and vegetable consumption was ‘healthy’ (N = 46). Adults’ knowledge was also
good, including restricting fatty foods, promoting fruit and vegetable intake, and maintaining a balanced diet. The
important role parents play in children’s eating behaviours and food intake was evident. The emerging themes
relating to barriers to healthy eating showed that external drivers such as advertising, the preferred sensory
experience of “unhealthy” foods, and food being used as a reward may play a role in preventing healthy eating.
Conclusions: Data suggest that; knowledge related to diet composition was not a barrier per se to healthy eating,
and education showing how to translate knowledge into behavior or action is required. The key themes that
emerged through the focus groups and pen-profiling data analysis technique will be used to inform and tailor the
healthy eating component of the CHANGE! intervention study.
Trial registration: Current Controlled Trials ISRCTN03863885
Keywords: Nutrition, Childhood obesity, Pen-profiles, Health, School
In Silico and In Vitro Investigations of the Mutability of Disease-Causing Missense Mutation Sites in Spermine Synthase
Spermine synthase (SMS) is a key enzyme controlling the concentration of spermidine and spermine in the cell. The importance of SMS is manifested by the fact that single missense mutations were found to cause Snyder-Robinson Syndrome (SRS). At the same time, currently there are no non-synonymous single nucleoside polymorphisms, nsSNPs (harmless mutations), found in SMS, which may imply that the SMS does not tolerate amino acid substitutions, i.e. is not mutable.To investigate the mutability of the SMS, we carried out in silico analysis and in vitro experiments of the effects of amino acid substitutions at the missense mutation sites (G56, V132 and I150) that have been shown to cause SRS. Our investigation showed that the mutation sites have different degree of mutability depending on their structural micro-environment and involvement in the function and structural integrity of the SMS. It was found that the I150 site does not tolerate any mutation, while V132, despite its key position at the interface of SMS dimer, is quite mutable. The G56 site is in the middle of the spectra, but still quite sensitive to charge residue replacement.The performed analysis showed that mutability depends on the detail of the structural and functional factors and cannot be predicted based on conservation of wild type properties alone. Also, harmless nsSNPs can be expected to occur even at sites at which missense mutations were found to cause diseases
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