5-HT2C Receptors Localize to Dopamine and GABA Neurons in the Rat Mesoaccumbens Pathway

The serotonin 5-HT2C receptor (5-HT2CR) is localized to the limbic-corticostriatal circuit, which plays an integral role in mediating attention, motivation, cognition, and reward processes. The 5-HT2CR is linked to modulation of mesoaccumbens dopamine neurotransmission via an activation of γ-aminobutyric acid (GABA) neurons in the ventral tegmental area (VTA). However, we recently demonstrated the expression of the 5-HT2CR within dopamine VTA neurons suggesting the possibility of a direct influence of the 5-HT2CR upon mesoaccumbens dopamine output. Here, we employed double-label fluorescence immunochemistry with the synthetic enzymes for dopamine (tyrosine hydroxylase; TH) and GABA (glutamic acid decarboxylase isoform 67; GAD-67) and retrograde tract tracing with FluoroGold (FG) to uncover whether dopamine and GABA VTA neurons that possess 5-HT2CR innervate the nucleus accumbens (NAc). The highest numbers of FG-labeled cells were detected in the middle versus rostral and caudal levels of the VTA, and included a subset of TH- and GAD-67 immunoreactive cells, of which >50% also contained 5-HT2CR immunoreactivity. Thus, we demonstrate for the first time that the 5-HT2CR colocalizes in DA and GABA VTA neurons which project to the NAc, describe in detail the distribution of NAc-projecting GABA VTA neurons, and identify the colocalization of TH and GAD-67 in the same NAc-projecting VTA neurons. These data suggest that the 5-HT2CR may exert direct influence upon both dopamine and GABA VTA output to the NAc. Further, the indication that a proportion of NAc-projecting VTA neurons synthesize and potentially release both dopamine and GABA adds intriguing complexity to the framework of the VTA and its postulated neuroanatomical roles

Characterization of Bacterial Magnetic Nanostructures Using High-Resolution Transmission Electron Microscopy and Off-Axis Electron Holography

Magnetotactic bacteria can be regarded as model systems for studying the structural, chemical, and magnetic properties of arrangements of ferrimagnetic iron oxide and sulfide nanocrystals. The aim of the present chapter is to show how the size, shape, crystal structure, crystallographic orientation, and spatial arrangement of bacterial magnetite (Fe 3 O 4) and greigite (Fe3S4) crystals affect their magnetic properties. We present recent results obtained using transmission electron microscopy (TEM) techniques, including high-resolution TEM imaging and off-axis electron holography

Spatial distribution of carbon dust in the early solar nebula and the carbon content of planetesimals

A high fraction of carbon bound in solid carbonaceous material is observed to exist in bodies formed in the cold outskirts of the solar nebula, while bodies in the terrestrial planets region contain nearly none. We study the fate of the carbonaceous material during the spiral-in of matter as the sun accretes matter from the solar nebula. From observational data on the composition of the dust component in comets and interplanetary dust particles, and from data on pyrolysis experiments, we construct a model for the composition of the pristine carbonaceous material in the outer parts of the solar nebula. We study the pyrolysis of the refractory and volatile organic component and the concomitant release of high-molecular-weight hydrocarbons under quiescent conditions of disk evolution where matter migrates inwards. We also study the decomposition and oxidation of the carbonaceous material during violent flash heating events, which are thought to be responsible for the formation of chondrules. It is found that the complex hydrocarbon components are removed from the solid disk matter at temperatures between 250 and 400 K, while the amorphous carbon component survives up to 1200 K. Without efficient carbon destruction during flash-heating associated with chondrule formation the carbon abundance of terrestrial planets, except for Mercury, would be not as low as it is found in cosmochemical studies. Chondrule formation seems to be a process that is crucial for the carbon-poor composition of the material of terrestrial planets.Comment: 17 pages, 7 figures, accepted by Astronomy & Astrophysic