Eclipsing Binaries in Open Clusters

Detached eclipsing binaries are very useful objects for calibrating theoretical stellar models and checking their predictions. Detached eclipsing binaries in open clusters are particularly important because of the additional constraints on their age and chemical composition from their membership of the cluster. I compile a list containing absolute parameters of well-studied eclipsing binaries in open clusters, and present new observational data on the B-type systems V1481 Cyg and V2263 Cyg which are members of the young open cluster NGC 7128.Comment: 4 pages, 2 colour figures. Poster presentation for IAUS 240 (Binary Stars as Critical Tools and Tests in Contemporary Astrophysics), Prague, August 2006. The poster itself can be dowloaded in ppt and pdf versions from http://www.astro.keele.ac.uk/~jkt/pubs.htm

High-throughput screening of monoclonal antibodies against plant cell wall glycans by hierarchical clustering of their carbohydrate microarray binding profiles

Antibody-producing hybridoma cell lines were created following immunisation with a crude extract of cell wall polymers from the plant Arabidopsis thaliana. In order to rapidly screen the specificities of individual monoclonal antibodies (mAbs), their binding to microarrays containing 50 cell wall glycans immobilized on nitrocellulose was assessed. Hierarchical clustering of microarray binding profiles from newly produced mAbs, together with the profiles for mAbs with previously defined specificities allowed the rapid assignments of mAb binding to antigen classes. mAb specificities were further investigated using subsequent immunochemical and biochemical analyses and two novel mAbs are described in detail. mAb LM13 binds to an arabinanase-sensitive pectic epitope and mAb LM14, binds to an epitope occurring on arabinogalactan-proteins. Both mAbs display novel patterns of recognition of cell walls in plant materials

SARS-CoV-2 Spike triggers barrier dysfunction and vascular leak via integrins and TGF-β signaling

Severe COVID-19 is associated with epithelial and endothelial barrier dysfunction within the lung as well as in distal organs. While it is appreciated that an exaggerated inflammatory response is associated with barrier dysfunction, the triggers of vascular leak are unclear. Here, we report that cell-intrinsic interactions between the Spike (S) glycoprotein of SARS-CoV-2 and epithelial/endothelial cells are sufficient to induce barrier dysfunction in vitro and vascular leak in vivo, independently of viral replication and the ACE2 receptor. We identify an S-triggered transcriptional response associated with extracellular matrix reorganization and TGF-β signaling. Using genetic knockouts and specific inhibitors, we demonstrate that glycosaminoglycans, integrins, and the TGF-β signaling axis are required for S-mediated barrier dysfunction. Notably, we show that SARS-CoV-2 infection caused leak in vivo, which was reduced by inhibiting integrins. Our findings offer mechanistic insight into SARS-CoV-2-triggered vascular leak, providing a starting point for development of therapies targeting COVID-19

The Scientific Foundations of Forecasting Magnetospheric Space Weather

The magnetosphere is the lens through which solar space weather phenomena are focused and directed towards the Earth. In particular, the non-linear interaction of the solar wind with the Earth's magnetic field leads to the formation of highly inhomogenous electrical currents in the ionosphere which can ultimately result in damage to and problems with the operation of power distribution networks. Since electric power is the fundamental cornerstone of modern life, the interruption of power is the primary pathway by which space weather has impact on human activity and technology. Consequently, in the context of space weather, it is the ability to predict geomagnetic activity that is of key importance. This is usually stated in terms of geomagnetic storms, but we argue that in fact it is the substorm phenomenon which contains the crucial physics, and therefore prediction of substorm occurrence, severity and duration, either within the context of a longer-lasting geomagnetic storm, but potentially also as an isolated event, is of critical importance. Here we review the physics of the magnetosphere in the frame of space weather forecasting, focusing on recent results, current understanding, and an assessment of probable future developments.Peer reviewe

Lessons learned from practical approaches to reconcile mismatches between biological population structure and stock units of marine fish

Recent advances in the application of stock identification methods have revealed inconsistencies between the spatial structure of biological populations and the definition of stock units used in assessment and management. From a fisheries management perspective, stocks are typically assumed to be discrete units with homogeneous vital rates that can be exploited independently of each other. However, the unit stock assumption is often violated leading to spatial mismatches that can bias stock assessment and impede sustainable fisheries management. The primary ecological concern is the potential for overexploitation of unique spawning components, which can lead to loss of productivity and reduced biodiversity along with destabilization of local and regional stock dynamics. Furthermore, ignoring complex population structure and stock connectivity can lead to misperception of the magnitude of fish productivity, which can translate to suboptimal utilization of the resource. We describe approaches that are currently being applied to improve the assessment and management process for marine fish in situations where complex spatial structure has led to an observed mismatch between the scale of biological populations and spatially-defined stock units. The approaches include: (i) status quo management, (ii) "weakest link" management, (iii) spatial and temporal closures, (iv) stock composition analysis, and (v) alteration of stock boundaries. We highlight case studies in the North Atlantic that illustrate each approach and synthesize the lessons learned from these real-world applications. Alignment of biological and management units requires continual monitoring through the application of stock identification methods in conjunction with responsive management to preserve biocomplexity and the natural stability and resilience of fish species.</p

Recruitment decline in North Sea herring is accompanied by reduced larval growth rates

The stock of North Sea autumn spawning herring Clupea harengus (L.) has shown an unprecedented 10 yr sequence of sharply reduced recruitment, in spite of a high spawning biomass. Recent work has identified this below-expected recruitment as being determined during the larval phase: however, the underlying mechanism remains elusive. In this study we analysed archived larval samples captured before and after the onset of the reduced survival to test the hypothesis of a concurrent change in the larval growth rate. Individual larval growth rates, averaged over the 21 d prior to capture, were estimated for ~200 larvae from 4 different years using a model-based analysis of otolith ring widths. Hydrographic backtracking models complemented the otolith analysis by reconstructing the environmental history and spawning origin of each larva. A significant reduction in net larval growth rate of 8%, concurrent with the reduced larval survival and recruitment, was identified: after correcting for the effect of other explanatory variables (e.g. temperature changes), the gross reduction was found to be 12%. This reduction is most probably due to changes in either the amount or quality of available food. The study demonstrates the potential of coupling 2 different techniques for affording new insights into fish early life history: otolith microstructure analysis and hydrographic modelling. Finally, the study provides a novel indication of the association between reduced growth and larvae survival, thereby narrowing the range of potential mechanisms underlying the observed reduction in the recruitment of North Sea autumn spawning herring