132 research outputs found
The synthesis of recombinant membrane proteins in yeast for structural studies
Historically, recombinant membrane protein production has been a major challenge meaning that many fewer membrane protein structures have been published than those of soluble proteins. However, there has been a recent, almost exponential increase in the number of membrane protein structures being deposited in the Protein Data Bank. This suggests that empirical methods are now available that can ensure the required protein supply for these difficult targets. This review focuses on methods that are available for protein production in yeast, which is an important source of recombinant eukaryotic membrane proteins. We provide an overview of approaches to optimize the expression plasmid, host cell and culture conditions, as well as the extraction and purification of functional protein for crystallization trials in preparation for structural studies
Role of electrostatic interactions in amyloid beta-protein (Abeta) oligomer formation: A discrete molecular dynamics study
Pathological folding and oligomer formation of the amyloid beta-protein
(Abeta) are widely perceived as central to Alzheimer's disease (AD).
Experimental approaches to study Abeta self-assembly are problematic, because
most relevant aggregates are quasi-stable and inhomogeneous. We apply a
discrete molecular dynamics (DMD) approach combined with a four-bead protein
model to study oligomer formation of the amyloid beta-protein (Abeta). We
address the differences between the two most common Abeta alloforms, Abeta40
and Abeta42, which oligomerize differently in vitro. We study how the presence
of electrostatic interactions (EIs) between pairs of charged amino acids
affects Abeta40 and Abeta42 oligomer formation. Our results indicate that EIs
promote formation of larger oligomers in both Abeta40 and Abeta42. The Abeta40
size distribution remains unimodal, whereas the Abeta42 distribution is
trimodal, as observed experimentally. Abeta42 folded structure is characterized
by a turn in the C-terminus that is not present in Abeta40. We show that the
same C-terminal region is also responsible for the strongest intermolecular
contacts in Abeta42 pentamers and larger oligomers. Our results suggest that
this C-terminal region plays a key role in the formation of Abeta42 oligomers
and the relative importance of this region increases in the presence of EIs.
These results suggest that inhibitors targeting the C-terminal region of
Abeta42 oligomers may be able to prevent oligomer formation or structurally
modify the assemblies to reduce their toxicity.Comment: Accepted for publication at Biophysical Journa
Generalizations of Gronwall-Bihari Inequalities on Time Scales
We establish some nonlinear integral inequalities for functions defined on a
time scale. The results extend some previous Gronwall and Bihari type
inequalities on time scales. Some examples of time scales for which our results
can be applied are provided. An application to the qualitative analysis of a
nonlinear dynamic equation is discussed.Comment: This is a preprint of an article accepted (16/May/2008) for
publication in the "Journal of Difference Equations and Applications"; J.
Difference Equ. Appl. is available online at http://www.informaworld.co
A signal theory approach to support vector classification: the sinc kernel
Fourier-based regularisation is considered for the support vector machine classification problem over absolutely integrable loss functions. By invoking the modest assumption that the decision function belongs to a Paley–Wiener space, it is shown that the classification problem can be developed in the context of signal theory. Furthermore, by employing the Paley–Wiener reproducing kernel, namely the sinc function, it is shown that a principled and finite kernel hyper-parameter search space can be discerned, a priori. Subsequent simulations performed on a commonly-available hyperspectral image data set reveal that the approach yields results that surpass state-of-the-art benchmarks
Causal categories: relativistically interacting processes
A symmetric monoidal category naturally arises as the mathematical structure
that organizes physical systems, processes, and composition thereof, both
sequentially and in parallel. This structure admits a purely graphical
calculus. This paper is concerned with the encoding of a fixed causal structure
within a symmetric monoidal category: causal dependencies will correspond to
topological connectedness in the graphical language. We show that correlations,
either classical or quantum, force terminality of the tensor unit. We also show
that well-definedness of the concept of a global state forces the monoidal
product to be only partially defined, which in turn results in a relativistic
covariance theorem. Except for these assumptions, at no stage do we assume
anything more than purely compositional symmetric-monoidal categorical
structure. We cast these two structural results in terms of a mathematical
entity, which we call a `causal category'. We provide methods of constructing
causal categories, and we study the consequences of these methods for the
general framework of categorical quantum mechanics.Comment: 43 pages, lots of figure
Sum rules and energy scales in the high-temperature superconductor YBa2Cu3O6+x
The Ferrell-Glover-Tinkham (FGT) sum rule has been applied to the temperature
dependence of the in-plane optical conductivity of optimally-doped
YBa_2Cu_3O_{6.95} and underdoped YBa_2Cu_3O_{6.60}. Within the accuracy of the
experiment, the sum rule is obeyed in both materials. However, the energy scale
\omega_c required to recover the full strength of the superfluid \rho_s in the
two materials is dramatically different; \omega_c \simeq 800 cm^{-1} in the
optimally doped system (close to twice the maximum of the superconducting gap,
2\Delta_0), but \omega_c \gtrsim 5000 cm^{-1} in the underdoped system. In both
materials, the normal-state scattering rate close to the critical temperature
is small, \Gamma < 2\Delta_0, so that the materials are not in the dirty limit
and the relevant energy scale for \rho_s in a BCS material should be twice the
energy gap. The FGT sum rule in the optimally-doped material suggests that the
majority of the spectral weight of the condensate comes from energies below
2\Delta_0, which is consistent with a BCS material in which the condensate
originates from a Fermi liquid normal state. In the underdoped material the
larger energy scale may be a result of the non-Fermi liquid nature of the
normal state. The dramatically different energy scales suggest that the nature
of the normal state creates specific conditions for observing the different
aspects of what is presumably a central mechanism for superconductivity in
these materials.Comment: RevTeX 4 file, 9 pages with 7 embedded eps figure
Neutrinoless double-beta decay and effective field theory
We analyze neutrinoless double -decay (\nbb-decay) mediated by heavy
particles from the standpoint of effective field theory. We show how symmetries
of the \nbb-decay quark operators arising in a given particle physics model
determine the form of the corresponding effective, hadronic operators. We
classify the latter according to their symmetry transformation properties as
well as the order at which they appear in a derivative expansion. We apply this
framework to several particle physics models, including R-parity violating
supersymmetry (RPV SUSY) and the left-right symmetric model (LRSM) with mixing
and a right-handed Majorana neutrino. We show that, in general, the pion
exchange contributions to \nbb-decay dominate over the short-range
four-nucleon operators. This confirms previously published RPV SUSY results and
allows us to derive new constraints on the masses in the LRSM. In particular,
we show how a non-zero mixing angle in the left-right symmetry model
produces a new potentially dominant contribution to \nbb-decay that
substantially modifies previous limits on the masses of the right-handed
neutrino and boson stemming from constraints from \nbb-decay and vacuum
stability requirements.Comment: 37 pages. Accepted for publication in PR
Microwave determination of the quasiparticle scattering time in YBa2Cu3O6.95
We report microwave surface resistance (Rs) measurements on two very-high-quality YBa2Cu3O6.95 crystals which exhibit extremely low residual loss at 1.2 K (2-6 μΩ at 2 GHz), a broad, reproducible peak at around 38 K, and a rapid increase in loss, by 4 orders of magnitude, between 80 and 93 K. These data provide one ingredient in the determination of the temperature dependence of the real part of the microwave conductivity, σ1(T), and of the quasiparticle scattering time. The other necessary ingredient is an accurate knowledge of the magnitude and temperature dependence of the London penetration depth, λ(T). This is derived from published data, from microwave data of Anlage, Langley, and co-workers and from, high-quality μSR data. We infer, from a careful analysis of all available data, that λ2(0)/λ2(T) is well approximated by the simple function 1-t2, where t=T/Tc, and that the low-temperature data are incompatible with the existence of an s-wave, BCS-like gap. Combining the Rs and λ(T) data, we find that σ1(T), has a broad peak around 32 K with a value about 20 times that at Tc. Using a generalized two-fluid model, we extract the temperature dependence of the quasiparticle scattering rate which follows an exponential law, exp(T/T0), where T0≊12 K, for T between 15 and 84 K. Such a temperature dependence has previously been observed in measurements of the nuclear spin-lattice relaxation rate. Both the uncertainties in our analysis and the implications for the mechanism of high-temperature superconductivity are discussed
Avaliação do impacto da suplementação alimentar a gestantes no cotrole do baixo peso ao nascer no município de São Paulo, SP (Brasil)
A partir de estudo realizado em oito grandes maternidades do Município de São Paulo, SP (Brasil) que atendem clientela predominantemente de baixo nível sócio-econômico, objetivou-se analisar o impacto da suplementação alimentar durante a assistência pré-natal sobre a incidência de recém-nascidos de baixo peso ao nascer (peso < 2.500 g). Foram envolvidos no estudo 1.060 recém-nascidos de mães que receberam suplementação e 664 recém-nascidos de mães que não a receberam. Ã incidência de baixo peso ao nascer foi de cerca de 11%, considerada elevada e semelhante em ambos os grupos de recém-nascidos. A análise multivariada, realizada para controlar eventuais diferenças entre os grupos, que não a condição de suplementação, descartou qualquer associação significativa entre suplementação e peso ao nascer e revelou, por outro lado, que tabagismo e morbidade na gestação e determinadas características antropométricas e reprodutivas da mãe, prévias à gestação, são importantes fatores de risco para o baixo peso ao nascer. A aparente explicação para a ausência de impacto da suplementação alimentar na população estudada parece residir não na quantidade insuficiente da suplementação alimentar oferecida (370 Kcal/dia), mas no predomínio de fatores não alimentares na determinação do baixo peso ao nascer. São formuladas recomendações quanto ao controle do baixo peso ao nascer no contexto estudado
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