91 research outputs found
Dynamics of ions in the selectivity filter of the KcsA channel
The statistical and dynamical properties of ions in the selectivity filter of the KcsA ion channel are considered on the basis of molecular dynamics (MD) simulations of the KcsA protein embedded in a lipid membrane surrounded by an ionic solution. A new approach to the derivation of a Brownian dynamics (BD) model of ion permeation through the filter is discussed, based on unbiased MD simulations. It is shown that depending on additional assumptions, ion’s dynamics can be described either by under-damped Langevin equation with constant damping and white noise or by Langevin equation with a fractional memory kernel. A comparison of the potential of the mean force derived from unbiased MD simulations with the potential produced by the umbrella sampling method demonstrates significant differences in these potentials. The origin of these differences is an open question that requires further clarifications
Homogeneous bubble nucleation limit of mercury under the normal working conditions of the planned European Spallation Source
In spallation neutron sources, liquid mercury is the subject of big thermal
and pressure shocks, upon adsorbing the proton beam. These changes can cause
unstable bubbles in the liquid, which can damage the structural material. While
there are methods to deal with the pressure shock, the local temperature shock
cannot be avoided. In our paper we calculated the work of the critical cluster
formation (i.e. for mercury micro-bubbles) together with the rate of their
formation (nucleation rate). It is shown that the homogeneous nucleation rates
are very low even after adsorbing several proton pulses, therefore the
probability of temperature induced homogeneous bubble nucleation is negligible.Comment: 22 Pages, 11 figures, one of them is colour, we plan to publish it in
Eur. Phys. J.
Female rat sexual behavior is unaffected by perinatal fluoxetine exposure
Serotonin plays an important role in adult female sexual behavior, however little is known about the influence of serotonin during early development on sexual functioning in adulthood. During early development, serotonin acts as neurotrophic factor, while it functions as a modulatory neurotransmitter in adulthood. The occurrence of serotonin release, could thus have different effects on behavioral outcomes, depending on the developmental period in which serotonin is released. Because serotonin is involved in the development of the HPG axis which is required for puberty establishment, serotonin could also alter expression patterns of for instance the estrogen receptor ɑ (ERɑ). The aim of our study was to investigate the effects of increased serotonin levels during early development on adult female rat sexual behavior during the full behavioral estrus in a seminatural environment. To do so, rats were perinatally exposed with the selective serotonin reuptake inhibitor (SSRI) fluoxetine (10 mg/kg FLX) and sexual performance was tested during adulthood. All facets of female sexual behavior between the first and last lordosis (behavioral estrus), and within each copulation bout of the behavioral estrus were analyzed. Besides the length and onset of the behavioral estrus and the sexual behaviors patterns, other social and conflict behavior were also investigated. In addition, we studied the effects of perinatal FLX exposure on ERɑ expression patterns in the medial preoptic nucleus, ventromedial nucleus of the hypothalamus, medial amygdala, bed nucleus of the stria terminalis, and the dorsal raphé nucleus. The results showed that perinatal fluoxetine exposure has no effect on adult female sexual behavior. The behavioral estrus of FLX-females had the same length and pattern as CTR-females. In addition, FLX- and CTR-females showed the same amount of paracopulatory behavior and lordosis, both during the full behavioral estrus and the "most active bout". Furthermore, no differences were found in the display of social and conflict behaviors, nor in ERɑ expression patterns in the brain. We conclude that increases in serotonin levels during early development do not have long-term consequences for female sexual behavior in adulthood.</p
Unidentified gamma-ray sources off the Galactic plane as low-mass microquasars?
A subset of the unidentified EGRET gamma-ray sources with no active galactic
nucleus or other conspicuous counterpart appears to be concentrated at medium
latitudes. Their long-term variability and their spatial distribution indicate
that they are distinct from the more persistent sources associated with the
nearby Gould Belt. They exhibit a large scale height of 1.3 +/- 0.6 kpc above
the Galactic plane. Potential counterparts for these sources include
microquasars accreting from a low-mass star and spewing a continuous jet.
Detailed calculations have been performed of the jet inverse Compton emission
in the radiation fields from the star, the accretion disc, and a hot corona.
Different jet Lorentz factors, powers, and aspect angles have been explored.
The up-scattered emission from the corona predominates below 100 MeV whereas
the disc and stellar contributions are preponderant at higher energies for
moderate (~15 deg) and small (~1 deg) aspect angles, respectively. Yet, unlike
in the high-mass, brighter versions of these systems, the external Compton
emission largely fails to produce the luminosities required for 5 to 10 kpc
distant EGRET sources. Synchrotron-self-Compton emission appears as a promising
alternative.Comment: 11 pages, 5 figures. Contributed paper to the "Multiwavelength
Approach to Unidentified Gamma-Ray Sources", Eds. K.S. Cheng & G.E. Romero,
to appear in Astrophysics and Space Science journa
Tomato: a crop species amenable to improvement by cellular and molecular methods
Tomato is a crop plant with a relatively small DNA content per haploid genome and a well developed genetics. Plant regeneration from explants and protoplasts is feasable which led to the development of efficient transformation procedures.
In view of the current data, the isolation of useful mutants at the cellular level probably will be of limited value in the genetic improvement of tomato. Protoplast fusion may lead to novel combinations of organelle and nuclear DNA (cybrids), whereas this technique also provides a means of introducing genetic information from alien species into tomato. Important developments have come from molecular approaches. Following the construction of an RFLP map, these RFLP markers can be used in tomato to tag quantitative traits bred in from related species. Both RFLP's and transposons are in the process of being used to clone desired genes for which no gene products are known. Cloned genes can be introduced and potentially improve specific properties of tomato especially those controlled by single genes. Recent results suggest that, in principle, phenotypic mutants can be created for cloned and characterized genes and will prove their value in further improving the cultivated tomato.
Observing Supermassive Black Holes across cosmic time: from phenomenology to physics
In the last decade, a combination of high sensitivity, high spatial
resolution observations and of coordinated multi-wavelength surveys has
revolutionized our view of extra-galactic black hole (BH) astrophysics. We now
know that supermassive black holes reside in the nuclei of almost every galaxy,
grow over cosmological times by accreting matter, interact and merge with each
other, and in the process liberate enormous amounts of energy that influence
dramatically the evolution of the surrounding gas and stars, providing a
powerful self-regulatory mechanism for galaxy formation. The different
energetic phenomena associated to growing black holes and Active Galactic
Nuclei (AGN), their cosmological evolution and the observational techniques
used to unveil them, are the subject of this chapter. In particular, I will
focus my attention on the connection between the theory of high-energy
astrophysical processes giving rise to the observed emission in AGN, the
observable imprints they leave at different wavelengths, and the methods used
to uncover them in a statistically robust way. I will show how such a combined
effort of theorists and observers have led us to unveil most of the SMBH growth
over a large fraction of the age of the Universe, but that nagging
uncertainties remain, preventing us from fully understating the exact role of
black holes in the complex process of galaxy and large-scale structure
formation, assembly and evolution.Comment: 46 pages, 21 figures. This review article appears as a chapter in the
book: "Astrophysical Black Holes", Haardt, F., Gorini, V., Moschella, U and
Treves A. (Eds), 2015, Springer International Publishing AG, Cha
- …