A promising new ELISA diagnostic test for cattle babesiosis based on Babesia bigemina Apical Membrane Antigen-1.

Babesiosis due to Babesia bigemina is a relevant tick‑borne disease, affecting cattle worldwide. Many surface proteins of the pathogen including the Apical Membrane Antigen 1 (AMA‑1) ‑ have been analysed for vaccine and diagnostic purposes. This study focused on B. bigemina AMA‑1 and on its use for the assessment of diagnostic tests. After bioinformatic analyses, AMA‑1 codifying region was amplified and cloned into an expression vector used to induce protein synthesis in Escherichia coli cells. AMA‑1 was purified by affinity chromatography and used to set up the best condition for an ELISA protocol. Bovine field sera positive to B. bigemina were used to evaluate the presence of anti‑AMA‑1 antibodies. In order to verify the assay specificity, sera positive to Babesia bovis or to the piroplasm Theileria annulata were also included. Significant differences were obtained between sera negative to both B. bigemina and B. bovis and samples positive to B. bigemina, to B. bovis or to both pathogens. No significant reaction was observed with T. annulata positive sera. The results showed that AMA‑1 protein is suitable to be used as antigen in diagnostic assays for babesiosis diagnosis in cattle, as it does not show any cross reaction with anti-T. annulata antibodies

Palmitoylethanolamide is a disease-modifying agent in peripheral neuropathy : pain relief and neuroprotection share a PPAR-alpha-mediated mechanism

Peer reviewedPublisher PD

An overview of the EXTraS project: Exploring the X-ray Transient and Variable Sky

The EXTraS project (Exploring the X-ray Transient and variable Sky) will harvest the hitherto unexplored temporal domain information buried in the serendipitous data collected by the European Photon Imaging Camera (EPIC) instrument onboard the ESA XMM-Newton X-ray observatory since its launch. This will include a search for fast transients, as well as a search and characterization of variability (both periodic and aperiodic) in hundreds of thousands of sources spanning more than nine orders of magnitude in time scale and six orders of magnitude in flux. X-ray results will be complemented by multiwavelength characterization of new discoveries. Phenomenological classification of variable sources will also be performed. All our results will be made available to the community. A didactic program in selected High Schools in Italy, Germany and the UK will also be implemented. The EXTraS project (2014-2016), funded within the EU/FP7 framework, is carried out by a collaboration including INAF (Italy), IUSS (Italy), CNR/IMATI (Italy), University of Leicester (UK), MPE (Germany) and ECAP (Germany).Comment: 6 pages, 1 figure. Proceedings of "Swift: 10 years of Discovery", to appear in Po

Experimental Signals of Phase Transition

The connection between the thermodynamics of charged finite nuclear systems and the asymptotically measured partitions is presented. Some open questions, concerning in particular equilibrium partitions are discussed. We show a detailed comparison of the decay patterns in Au+ C,Cu,Au central collisions and in Au quasi-projectile events. Observation of abnormally large fluctuations in carefully selected samples of data is reported as an indication of a first order phase transition (negative heat capacity) in the nuclear equation of state.Comment: 8 pages, 8th International Conference on Nucleus-Nucleus Collisions, Moscow 200

Negative heat capacity in the critical region of nuclear fragmentation: an experimental evidence of the liquid-gas phase transition

An experimental indication of negative heat capacity in excited nuclear systems is inferred from the event by event study of energy fluctuations in $Au$ quasi-projectile sources formed in $Au+Au$ collisions at 35 A.MeV. The excited source configuration is reconstructed through a calorimetric analysis of its de-excitation products. Fragment partitions show signs of a critical behavior at about 5 A.MeV excitation energy. In the same energy range the heat capacity shows a negative branch providing a direct evidence of a first order liquid gas phase transition.Comment: 4 Postscript figures, submitted to Phys. Rev. Lett. on 14-apr-199

Impact of a nursing information system in clinical practice: a longitudinal study project

Background: The implementation of adequate clinical information systems helps to deal with the immense flow of health data to ensure the continuity of care and access to a safe and high-quality healthcare system. Currently there is an increasing awareness of the importance of evaluating and measuring the impact of such systems in clinical practice. Implementations often fail, due to inadequate interaction between technology and human elements. Methods: This article describes a research project aimed at evaluating the impact of a clinical nursing information system (CNIS), called Professional Assessment Instrument (PAI), in clinical practice. The study will evaluate PAI Quality, Nurses Satisfaction, PAI Use, Nurses and Environment Characteristics, Net Benefits and Nurses’ Experiences related to the PAI use. A theoretical model developed for this research will guide the study. A quali-quantitative longitudinal design will be performed involving two hospitals over a 9-month period. To measure different dimensions that affect the success/failure of CNIS we will use different tools/ methods of data collection (questionnaires, psychometric tools, surveys and focus groups). Expected Results: This study will evaluate the impact of a CNIS in hospitals providing an overview of the factors which can help and hinder the implementation of an information system. Conclusions: The results of the study will support interventions to improve and implement clinical information systems designed to computerize nursing data, with positive effects on public health and research in general, providing further evidence for health policy

Size and asymmetry of the reaction entrance channel: influence on the probability of neck production

The results of experiments performed to investigate the Ni+Al, Ni+Ni, Ni+Ag reactions at 30 MeV/nucleon are presented. From the study of dissipative midperipheral collisions, it has been possible to detect events in which Intermediate Mass Fragments (IMF) production takes place. The decay of a quasi-projectile has been identified; its excitation energy leads to a multifragmentation totally described in terms of a statistical disassembly of a thermalized system (T$\simeq$4 MeV, E$^*\simeq$4 MeV/nucleon). Moreover, for the systems Ni+Ni, Ni+Ag, in the same nuclear reaction, a source with velocity intermediate between that of the quasi-projectile and that of the quasi-target, emitting IMF, is observed. The fragments produced by this source are more neutron rich than the average matter of the overall system, and have a charge distribution different, with respect to those statistically emitted from the quasi-projectile. The above features can be considered as a signature of the dynamical origin of the midvelocity emission. The results of this analysis show that IMF can be produced via different mechanisms simultaneously present within the same collision. Moreover, once fixed the characteristics of the quasi-projectile in the three considered reactions (in size, excitation energy and temperature), one observes that the probability of a partner IMF production via dynamical mechanism has a threshold (not present in the Ni+Al case) and increases with the size of the target nucleus.Comment: 16 pages, 7 figures, accepted for publication on Nuclear Physics

Histamine plasma levels and elimination diet in chronic idiopathic urticaria

Abstract: Objective: The aim of this study was to evaluate the effects of an oligoantigenic and histamine-free diet on patients affected with chronic idiopathic urticaria (CIU). Design: Ten patients with chronic idiopathic urticaria were prescribed an oligoantigenic and histamine-free diet for 21 days, followed by serial and controlled reintroduction of foods during a further 70 days. Modification in clinical illness as well as histamine plasma levels, post-heparin plasma diamine oxidase (DAO) and intestinal permeability were evaluated. Results: The oligoantigenic and histamine-free diet induced a significant improvement of symptoms (P < 0.05). Moreover, CIU patients on free diet showed higher histamine plasma levels (P < 0.05 vs post-diet and vs controls) that fell to control levels during the oligoantigenic and histamine-free diet. Post-heparin plasma diamine oxidase values were slightly reduced and were unchanged during the diet as well as intestinal permeability, which was always normal in all patients. Conclusions: These data suggest that histamine plays a major role in chronic idiopathic urticaria. The finding of normal intestinal permeability suggests that a morphological damage of intestinal mucosa should be excluded in these patients. However, the presence of low levels of post-heparin plasma diamine oxidase may indicate a subclinical impairment of small bowel enterocyte function that could induce a higher sensitivity to histamine-rich or histamine-producing food

Discovery of periodic dips in the brightest hard X-ray source of M31 with EXTraS

We performed a search for eclipsing and dipping sources in the archive of the EXTraS project - a systematic characterization of the temporal behaviour of XMM-Newton point sources. We discovered dips in the X-ray light curve of 3XMM J004232.1+411314, which has been recently associated with the hard X-ray source dominating the emission of M31. A systematic analysis of XMM-Newton observations revealed 13 dips in 40 observations (total exposure time $\sim$0.8 Ms). Among them, four observations show two dips, separated by $\sim$4.01 hr. Dip depths and durations are variable. The dips occur only during low-luminosity states (L$_{0.2-12}<1\times10^{38}$ erg s$^{-1}$), while the source reaches L$_{0.2-12}\sim2.8\times10^{38}$ erg s$^{-1}$. We propose this system to be a new dipping Low-Mass X-ray Binary in M31 seen at high inclination (60$^{\circ}$-80$^{\circ}$), the observed dipping periodicity is the orbital period of the system. A blue HST source within the Chandra error circle is the most likely optical counterpart of the accretion disk. The high luminosity of the system makes it the most luminous dipper known to date.Comment: 11 pages, 2 figures, 5 tables, accepted for publication in ApJ

On the reliability of negative heat capacity measurements

A global protocol for the thermostatistical analysis of hot nuclear sources is discussed. Within our method of minimization of variances we show that the abnormal kinetic energy fluctuation signal recently reported in different experimental data (M.D'Agostino et al.-Phys. Lett. B 473 (2000) 219, N. Le Neindre et al.- contr. to the XXXVIII Bormio Winter Meeting on Nucl. Phys. (2001) 404) is a genuine signal of a first order phase transition in a finite system.Comment: 15 Postscript figures, submitted to NUCL. Phys. A on 24-apr-200