The contribution of cohesin-SA1 to gene expression and chromatin architecture in two murine tissues

Cohesin, which in somatic vertebrate cells consists of SMC1, SMC3, RAD21 and either SA1 or SA2, mediates higher-order chromatin organization. To determine how cohesin contributes to the establishment of tissue-specific transcriptional programs, we compared genome-wide cohesin distribution, gene expression and chromatin architecture in cerebral cortex and pancreas from adult mice. More than one third of cohesin binding sites differ between the two tissues and these show reduced overlap with CCCTC-binding factor (CTCF) and are enriched at the regulatory regions of tissue-specific genes. Cohesin/CTCF sites at active enhancers and promoters contain, at least, cohesin-SA1. Analyses of chromatin contacts at the Protocadherin (Pcdh) and Regenerating islet-derived (Reg) gene clusters, mostly expressed in brain and pancreas, respectively, revealed remarkable differences that correlate with the presence of cohesin. We could not detect significant changes in the chromatin contacts at the Pcdh locus when comparing brains from wild-type and SA1 null embryos. In contrast, reduced dosage of SA1 altered the architecture of the Reg locus and decreased the expression of Reg genes in the pancreas of SA1 heterozygous mice. Given the role of Reg proteins in inflammation, such reduction may contribute to the increased incidence of pancreatic cancer observed in these animals.The Spanish Ministry of Economy and Competitiveness (MINECO) [BFU2013-48481-R to A.L.]; 'Ramon y Cajal' Contract [RYC-2010-06122 to A.C.]; Fundacion La Caixa [PhD Fellowship to S.R.]. Funding for open access charge: The Spanish Ministry of Economy and Competitiveness (MINECO) [BFU2013-48481-R]S

Prototipo de control de fauna de pequeño tamaño basado en Raspberry Pi y el uso de identificación RFID

El proyecto implementa un sistema en Raspberry Pi, que mediante un lector RFID y una cámara identifica al ave que porta la etiqueta, guardando sus datos y su fotografía en una base de datos.<br /

Procedimientos innovadores utilizados en las prácticas externas para el desarrollo de la identidad profesional

Las prácticas profesionales externas constituyen un momento clave en el proceso de desarrollo y afianzamiento de la identidad profesional del futuro profesional de la educación social. La investigación realizada, centrada en la asignatura de Prácticas Profesionales III del Grado de Educación Social de la UNED, pretende determinar la influencia que la introducción de técnicas e instrumentos cualitativos durante la realización de éstas, ejerce en la identidad profesional de los estudiantes. Así, mediante una metodología mixta, los resultados obtenidos en la investigación efectuada durante el curso 2016-17 evidencian que la utilización de la autobiografía, grupos de discusión y cuestionarios de valoración de la práctica durante la estancia en la institución de prácticas, facilitan la reflexión de los propios estudiantes y la toma de conciencia sobre su avance formativo, aspectos que inciden directamente en la adquisición y reforzamiento de su identidad profesional desde tres dimensiones: la autoimagen, la misión social y el compromiso ético con la profesión

Cold-water corals and deep-sea sponges by-catch mitigation: Dealing with groundfish survey data in the management of the northwest Atlantic Ocean high seas fisheries

The integration of survey data in the processes of the Regional Fisheries Management Organisations is a key step for conservation of deep-sea ecosystems and sustainable exploitation of deep-sea fisheries resources, including the mitigation of by-catch and discards of cold-water corals and deep-sea sponges, both considered by FAO as vulnerable marine ecosystems (VMEs) indicator species. Information on corals and sponges from annual bottom trawl groundfish surveys in areas beyond national jurisdictions has been integrated into the “ecosystem management cycle” of the Northwest Atlantic Fisheries Organization (NAFO). Survey data have improved our knowledge on VMEs identification, distribution and extent, and has led to the proposal and implementation of conservation and management measures. These data have particular relevance to delineate and refine the boundaries of areas closed to commercial bottom fishing (14 closures), in order to prevent significant adverse impacts on VMEs, according to the mandate of United Nations General Assembly Resolution 61/105. Considering the European groundfish surveys in the NAFO Regulatory Area (high seas) as a case study, the paper presents an overview of how invertebrate catch data have been integrated into the fisheries management process as a basis to the implementation of VMEs closed areas. Fishing closures are considered effective spatial management measures to avoid by-catch and discards of cold-water corals and deep-sea sponges in commercial bottom fishing, mitigating the adverse impacts on deep-sea ecosystemsVersión del editor1,86

Actividad alelopática de la cafeína en plántulas de trigo y lenteja

La cafeína es un alcaloide que posee efectos aleloquímicos y autotóxicos. En el presente trabajo se ha extraído cafeína de hojas de té, utilizando agua caliente alcalinizada con CaCO3, extracción con diclorometano y precipitación en mezcla de acetona caliente-hexano, y se ha comprobado la pureza de la cafeína extraída mediante técnicas cromatográficas y espectrofotométricas. Con el extracto obtenido se ha llevado a cabo un ensayo para valorar el efecto que ejerce este alcaloide sobre ciertos parámetros de crecimiento en trigo y lenteja. Mediante este procedimiento se pudo observar que la cafeína inhibía el crecimiento, tanto de la raíz como del tallo, en ambas especies, en un rango amplio de concentraciones, aunque este efecto fue mayor en las raíce

A theoretical exercise of Marine Spatial Planning in the Flemish Cap and Flemish Pass (NAFO Divs. 3LM): implications for fisheries management in the high seas

A theoretical exercise of Maritime Spatial Planning (MSP) is currently being conducted within the Regulatory Area of the Northwest Atlantic Fisheries Organization (NAFO), using the MESMA framework to assess whether the existing science base is sufficient to support a potential spatially managed area. The case study is located in the high seas within the Flemish Cap – Flemish Pass area. It includes cold-water coral and deep-sea sponge vulnerable marine ecosystems (VMEs), bottom fishing closed areas, and different blue economy activities, such as high seas fisheries and offshore oil and gas. The paper summarizes the context setting for MSP, in a theoretical scenario to accommodate an emergent offshore hydrocarbon exploration and exploitation, minimizing impacts on VMEs and existing high seas fisheries. Biophysical and socio-economic components of the ecosystem are mapped, including the spatial overlapping between new and traditional uses of the marine space, focused on potential conflicts user-user (e.g. hydrocarbon industry and deep-sea fisheries) or userenvironment (e.g. hydrocarbon industry and VMEs) and considering transboundary conflicts (e.g. recent oil spills). Current and potential management measures are described. This is followed by a discussion on the role and challenges of MSP in Areas Beyond National Jurisdiction, as lessons learned from the present exercise. Finally, the future work is briefly outlined

Fully-Convolutional Denoising Auto-Encoders for NILM in Large Non-Residential Buildings

[EN] Great concern regarding energy efficiency has led the research community to develop approaches which enhance the energy awareness by means of insightful representations. An example of intuitive energy representation is the parts-based representation provided by Non-Intrusive Load Monitoring (NILM) techniques which decompose non-measured individual loads from a single total measurement of the installation, resulting in more detailed information about how the energy is spent along the electrical system. Although there are previous works that have achieved important results on NILM, the majority of the NILM systems were only validated in residential buildings, leaving a niche for the study of energy disaggregation in non-residential buildings, which present a specific behavior. In this paper, we suggest a novel fully-convolutional denoising auto-encoder architecture (FCN-dAE) as a convenient NILM system for large non-residential buildings, and it is compared, in terms of particular aspects of large buildings, to previous denoising auto-encoder approaches (dAE) using real electrical consumption from a hospital facility. Furthermore, by means of three use cases, we show that our approach provides extra helpful funcionalities for energy management tasks in large buildings, such as meter replacement, gap filling or novelty detection.SIThis work was supported by the Principado de Asturias Government through the Predoctoral Grant “Severo Ochoa.” Paper no. TSG-00737-202