Genetic testing reveals some mislabeling but general compliance with a ban on herbivorous fish harvesting in Belize

Overfishing of herbivorous fishes is one of the primary causes of Caribbean coral reef decline. In Belize, herbivorous fishes comprised 28% of the catch from 2005 to 2008. In 2009, the Belize Fisheries Department implemented a national ban on herbivorous fish harvesting to mitigate high-macroalgal cover on much of the Belize Barrier Reef. However, compliance with this approach has not been evaluated. We assessed the proportion of herbivorous fish in local markets by genetically identifying fish fillets sold in five major towns in Belize from 2009 to 2011. We found that 5-7% of 111 fillets were identified as herbivorous fish and 32-51% were mislabeled. A 5-7% proportion of parrotfish in local markets suggests some ongoing parrotfish harvesting. However, our results suggest that the ban has reduced herbivorous fish harvesting and has the potential to help facilitate the restoration of coral reef ecosystems

A cross sectional study of the prevalence and associated risks for bursitis in 6250 weaner, grower and finisher pigs from 103 British pig farms

A cross-sectional study of 93 farms in England was carried out to estimate the prevalence and associated risk factors for bursitis. A total of 6250 pigs aged 6–22 weeks were examined for presence and severity of bursitis. Details of pen construction, pen quality and farm management were recorded including floor type, presence of bedding, condition of the floor and floor materials. The prevalence of bursitis was 41.2% and increased with each week of age (OR 1.1). Two-level logistic regression models were developed with the outcome as the proportion of pigs affected with bursitis in a pen. Pigs kept on soil floors with straw bedding were used as the reference level. In comparison with these soil floors, bursitis increased on concrete floors where the bedding was deep throughout (OR 4.6), deep in part (OR 3.7), and sparse throughout (OR 9.0), part slatted floors (OR 8.0), and fully slatted floors (OR 18.8). Slip or skid marks in the dunging area (OR 1.5), pigs observed slipping during the examination of the pen (OR 1.3) and wet floors (OR 3.6) were also associated with an increased risk of bursitis. The results indicate that bursitis is a common condition of growing pigs and that the associated risk factors for bursitis were a lack of bedding in the lying area, presence of voids and pen conditions which increased the likelihood of injury

2D and 3D Polar Plume Analysis from the Three Vantage Positions of STEREO/EUVI A, B, and SOHO/EIT

Polar plumes are seen as elongated objects starting at the solar polar regions. Here, we analyze these objects from a sequence of images taken simultaneously by the three spacecraft telescopes STEREO/EUVI A and B, and SOHO/EIT. We establish a method capable of automatically identifying plumes in solar EUV images close to the limb at 1.01 - 1.39 R in order to study their temporal evolution. This plume-identification method is based on a multiscale Hough-wavelet analysis. Then two methods to determined their 3D localization and structure are discussed: First, tomography using the filtered back-projection and including the differential rotation of the Sun and, secondly, conventional stereoscopic triangulation. We show that tomography and stereoscopy are complementary to study polar plumes. We also show that this systematic 2D identification and the proposed methods of 3D reconstruction are well suited, on one hand, to identify plumes individually and on the other hand, to analyze the distribution of plumes and inter-plume regions. Finally, the results are discussed focusing on the plume position with their cross-section area.Comment: 22 pages, 10 figures, Solar Physics articl

Carbon monoxide-releasing antibacterial molecules target respiration and global transcriptional regulators

Carbon monoxide, a classical respiratory inhibitor, also exerts vasodilatory, anti-inflammatory, and antiapoptotic effects. CO-releasing molecules have therapeutic value, increasing phagocytosis and reducing sepsis-induced lethality. Here we identify for the first time the bacterial targets of Ru(CO)(3)Cl(glycinate) (CORM-3), a ruthenium-based carbonyl that liberates CO rapidly under physiological conditions. Contrary to the expectation that CO would be preferentially inhibitory at low oxygen tensions or anaerobically, Escherichia coli cultures were also sensitive to CORM-3 at concentrations equimolar with oxygen. CORM-3, assayed as ruthenium, was taken up by bacteria and rapidly delivered CO intracellularly to terminal oxidases. Microarray analysis of CORM-3-treated cells revealed extensively modified gene expression, notably down-regulation of genes encoding key aerobic respiratory complexes. Genes involved in metal metabolism, homeostasis, or transport were also differentially expressed, and free intracellular zinc levels were elevated. Probabilistic modeling of transcriptomic data identified the global transcription regulators ArcA, CRP, Fis, FNR, Fur, BaeR, CpxR, and IHF as targets and potential CO sensors. Our discovery that CORM-3 is an effective inhibitor and global regulator of gene expression, especially under aerobic conditions, has important implications for administration of CO-releasing agents in sepsis and inflammatio

Resultados de la enseñanza de estrategias de lectura y escritura en la alfabetización temprana de niños con riesgo social

Introducción: La evidencia científica reciente destaca la importancia de la alfabetización temprana como fundamento del aprendizaje lector posterior, especialmente en niños en riesgo social, pero no existen las condiciones adecuadas para alcanzar su desarrollo. El objetivo del estudio fue evaluar los resultados de una intervención focalizada en el aprendizaje temprano de la lectura y la escritura de niños con riesgo social (5 años 3 meses promedio). Material y Métodos: Se evaluaron 339 niños(as) (132 grupo intervención/207 grupo comparación), al inicio y al fin del año escolar. Los profesores del grupo de intervención participaron en un programa de desarrollo profesional de un año que incluyó capacitación y acompañamiento para la aplicación de estrategias de enseñanza de la alfabetización temprana. Resultados: Se observó un resultado positivo en el conocimiento del alfabeto, la escritura emergente y el reconocimiento visual de palabras, no en comprensión oral. Discusión: Se contrastan los resultados con otros programas de intervención en niños con riesgo social, resaltando la importancia de la capacitación y el acompañamiento a los educadores

Beyond opioid prescribing: Evaluation of a substance use disorder curriculum for OBGYN residents

Objective Amidst the current opioid crisis, there is a need for better integration of substance use disorder screening and treatment across specialties. However, there is no consensus regarding how to best instruct OBGYN trainees in the clinical skills related to opioid and other substance use disorders (SUD). Study objectives were (1) to assess the effectiveness a SUD curriculum to improve self-reported competence among OBGYN residents and (2) to explore its effectiveness to improve attending evaluations of residents clinical skills as well as its feasibility and acceptability from the resident perspective. Methods A pilot 3-session curriculum was developed and adapted to SUD screening and treatment which included readings, didactics, and supervised outpatient clinical experiences for OBGYN post-graduate year 1 (PGY-1) residents rotating through an integrated OBGYNSUD clinic. Eighteen residents completed pre and post clinical skills self-Assessments (SUD screening, counseling, referring, Motivational Interviewing) using an adapted Zwisch Rating Scale (range 1 5). Scores were compared between time points using paired t-Tests. Subsamples also (a) were evaluated by the attending on three relevant Accreditation Council for Graduate Medical Education Milestones (ACGME) milestone sets using the web-based feedback program, myTIPreport (n = 10) and (b) completed a qualitative interview (n = 4). Results All PGY-1s (18/18) across three academic years completed the 3-session SUD curriculum. Clinical skill self-Assessments improved significantly in all areas [SUD Screening (2.44 (0.98) vs 3.56 (0.62), p = 0.01); Counseling (1.81 (0.71) vs 3.56 (0.51), p = .01; Referring (2.03 (0.74) vs 3.17 (0.71), p = .01; Motivational Interviewing (1.94 (1.06) vs 3.33 (0.69), p = .01)]. Milestone set levels assigned by attending evaluations (n = 10) also improved. Qualitative data (n = 4) revealed high acceptability; all curriculum components were viewed positively, and feedback was provided (e.g., desire for more patient exposures). Conclusion A pilot SUD curriculum tailored for OBGYN PGY-1 residents that goes beyond opioid prescribing to encompass SUD management is feasible, acceptable and likely effective at improving SUD core clinical skills

Healthcare-associated outbreak of meticillin-resistant Staphylococcus aureus bacteraemia: role of a cryptic variant of an epidemic clone

BACKGROUND New strains of meticillin-resistant Staphylococcus aureus (MRSA) may be associated with changes in rates of disease or clinical presentation. Conventional typing techniques may not detect new clonal variants that underlie changes in epidemiology or clinical phenotype. AIM To investigate the role of clonal variants of MRSA in an outbreak of MRSA bacteraemia at a hospital in England. METHODS Bacteraemia isolates of the major UK lineages (EMRSA-15 and -16) from before and after the outbreak were analysed by whole-genome sequencing in the context of epidemiological and clinical data. For comparison, EMRSA-15 and -16 isolates from another hospital in England were sequenced. A clonal variant of EMRSA-16 was identified at the outbreak hospital and a molecular signature test designed to distinguish variant isolates among further EMRSA-16 strains. FINDINGS By whole-genome sequencing, EMRSA-16 isolates during the outbreak showed strikingly low genetic diversity (P < 1 × 10(-6), Monte Carlo test), compared with EMRSA-15 and EMRSA-16 isolates from before the outbreak or the comparator hospital, demonstrating the emergence of a clonal variant. The variant was indistinguishable from the ancestral strain by conventional typing. This clonal variant accounted for 64/72 (89%) of EMRSA-16 bacteraemia isolates at the outbreak hospital from 2006. CONCLUSIONS Evolutionary changes in epidemic MRSA strains not detected by conventional typing may be associated with changes in disease epidemiology. Rapid and affordable technologies for whole-genome sequencing are becoming available with the potential to identify and track the emergence of variants of highly clonal organisms

Exceptional collections and D-branes probing toric singularities

We demonstrate that a strongly exceptional collection on a singular toric surface can be used to derive the gauge theory on a stack of D3-branes probing the Calabi-Yau singularity caused by the surface shrinking to zero size. A strongly exceptional collection, i.e., an ordered set of sheaves satisfying special mapping properties, gives a convenient basis of D-branes. We find such collections and analyze the gauge theories for weighted projective spaces, and many of the Y^{p,q} and L^{p,q,r} spaces. In particular, we prove the strong exceptionality for all p in the Y^{p,p-1} case, and similarly for the Y^{p,p-2r} case.Comment: 49 pages, 6 figures; v2 refs added; v3 published versio

Baryonic Generating Functions

We show how it is possible to use the plethystic program in order to compute baryonic generating functions that count BPS operators in the chiral ring of quiver gauge theories living on the world volume of D branes probing a non compact CY manifold. Special attention is given to the conifold theory and the orbifold C^2/Z_2 times C, where exact expressions for generating functions are given in detail. This paper solves a long standing problem for the combinatorics of quiver gauge theories with baryonic moduli spaces. It opens the way to a statistical analysis of quiver theories on baryonic branches. Surprisingly, the baryonic charge turns out to be the quantized Kahler modulus of the geometry.Comment: 44 pages, 7 figures; fonts change