1,420 research outputs found
A measurement of the cosmological mass density from clustering in the 2dF Galaxy Redshift Survey
The large-scale structure in the distribution of galaxies is thought to arise from the gravitational instability of small fluctuations in the initial density field of the Universe. A key test of this hypothesis is that forming superclusters of galaxies should generate a systematic infall of other galaxies. This would be evident in the pattern of recessional velocities, causing an anisotropy in the inferred spatial clustering of galaxies. Here we report a precise measurement of this clustering, using the redshifts of more than 141,000 galaxies from the two-degree-field (2dF) galaxy redshift survey. We determine the parameter β = Ω0.6/b = 0.43 +/- 0.07, where Ω is the total mass-density parameter of the Universe and b is a measure of the `bias' of the luminous galaxies in the survey. (Bias is the difference between the clustering of visible galaxies and of the total mass, most of which is dark.) Combined with the anisotropy of the cosmic microwave background, our results favour a low-density Universe with Ω ~ 0.3
The 2dF galaxy redshift survey: near-infrared galaxy luminosity functions
We combine the Two Micron All Sky Survey (2MASS) Extended Source Catalogue and the 2dF Galaxy Redshift Survey to produce an infrared selected galaxy catalogue with 17173 measured redshifts. We use this extensive data set to estimate the galaxy luminosity functions in the J- and KS-bands. The luminosity functions are fairly well fitted by Schechter functions with parameters MJ*-5logh=-22.36+/-0.02, αJ=-0.93+/-0.04, ΦJ*=0.0104+/-0.0016h3Mpc-3 in the J-band and MKS*-5logh=-23.44+/-0.03, αKS=-0.96+/-0.05, ΦKS*=0.0108+/-0.0016h3Mpc-3 in the KS-band (2MASS Kron magnitudes). These parameters are derived assuming a cosmological model with Ω0=0.3 and Λ0=0.7. With data sets of this size, systematic rather than random errors are the dominant source of uncertainty in the determination of the luminosity function. We carry out a careful investigation of possible systematic effects in our data. The surface brightness distribution of the sample shows no evidence that significant numbers of low surface brightness or compact galaxies are missed by the survey. We estimate the present-day distributions of bJ-KS and J-KS colours as a function of the absolute magnitude and use models of the galaxy stellar populations, constrained by the observed optical and infrared colours, to infer the galaxy stellar mass function. Integrated over all galaxy masses, this yields a total mass fraction in stars (in units of the critical mass density) of Ωstarsh=(1.6+/-0.24)×10-3 for a Kennicutt initial mass function (IMF) and Ωstarsh=(2.9+/-0.43)×10-3 for a Salpeter IMF. These values are consistent with those inferred from observational estimates of the total star formation history of the Universe provided that dust extinction corrections are modest
Cosmological Parameters Degeneracies and Non-Gaussian Halo Bias
We study the impact of the cosmological parameters uncertainties on the
measurements of primordial non-Gaussianity through the large-scale non-Gaussian
halo bias effect. While this is not expected to be an issue for the standard
LCDM model, it may not be the case for more general models that modify the
large-scale shape of the power spectrum. We consider the so-called local
non-Gaussianity model and forecasts from planned surveys, alone and combined
with a Planck CMB prior. In particular, we consider EUCLID- and LSST-like
surveys and forecast the correlations among and the running of the
spectral index , the dark energy equation of state , the effective
sound speed of dark energy perturbations , the total mass of massive
neutrinos , and the number of extra relativistic degrees of
freedom . Neglecting CMB information on and scales /Mpc, we find that, if is assumed to be known, the
uncertainty on cosmological parameters increases the error on by
10 to 30% depending on the survey. Thus the constraint is
remarkable robust to cosmological model uncertainties. On the other hand, if
is simultaneously constrained from the data, the
error increases by . Finally, future surveys which provide a large
sample of galaxies or galaxy clusters over a volume comparable to the Hubble
volume can measure primordial non-Gaussianity of the local form with a
marginalized 1-- error of the order , after
combination with CMB priors for the remaining cosmological parameters. These
results are competitive with CMB bispectrum constraints achievable with an
ideal CMB experiment.Comment: 17 pages, 1 figure added, typos corrected, comments added, matches
the published versio
A molecular switch in RCK2 triggers sodium-dependent activation of KNa.1 (KCNT1) potassium channels
The Na⁺-activated K⁺ channel KNa1.1, encoded by the KCNT1 gene, is an important regulator of neuronal excitability. How intracellular Na⁺ ions bind and increase channel activity is not well understood. Analysis of KNa1.1 channel structures indicate that there is a large twisting of the βN-αQ loop in the intracellular RCK2 domain between the inactive and Na⁺-activated conformations, with a lysine (K885, human subunit numbering) close enough to potentially form a salt bridge with an aspartate (D839) in βL in the Na⁺-activated state. Concurrently, an aspartate (D884) adjacent in the same loop adopts a position within a pocket formed by the βO strand. In carrying out mutagenesis and electrophysiology with human KNa1.1, we found alanine substitution of selected residues in these regions resulted in almost negligible currents in the presence of up to 40 mM intracellular Na⁺. The exception was D884A, which resulted in constitutively active channels in both the presence and absence of intracellular Na⁺. Further mutagenesis of this site revealed an amino acid size-dependent effect. Substitutions at this site by an amino acid smaller than aspartate (D884V) also yielded constitutively active KNa1.1, D884I had Na⁺-dependence similar to wild-type KNa1.1, whilst increasing the side chain size larger than aspartate (D884E or D884F) yielded channels that could not be activated by up to 40 mM intracellular Na⁺. We conclude that Na⁺ binding results in a conformational change that accommodates D884 in the βO pocket, which triggers further conformational changes in the RCK domains and channel activation
Chaperone-mediated native folding of a β-scorpion toxin in the periplasm of E.coli
Background: Animal neurotoxin peptides are valuable probes for investigating ion channel structure/function relationships and represent lead compounds for novel therapeutics and insecticides. However, misfolding and aggregation are common outcomes when toxins containing multiple disulfides are expressed in bacteria.
Methods: The ß-scorpion peptide toxin Bj-xtrIT from Hottentotta judaica and four chaperone enzymes (DsbA, DsbC, SurA and FkpA) were co-secreted into the oxidizing environment of the E.coli periplasm. Expressed Bj-xtrIT was purified and analyzed by HPLC and FPLC chromatography. Its thermostability was assessed using synchrotron radiation circular dichroism spectroscopy and its crystal structure was determined.
Results: Western blot analysis showed that robust expression was only achieved when cells co-expressed the chaperones. The purified samples were homogenous and monodisperse and the protein was thermostable. The crystal structure of the recombinant toxin confirmed that it adopts the native disulfide connectivity and fold.
Conclusions: The chaperones enabled correct folding of the four-disulfide-bridged Bj-xtrIT toxin. There was no apparent sub-population of misfolded Bj-xtrIT, which attests to the effectiveness of this expression method.
General Significance: We report the first example of a disulfide-linked scorpion toxin natively folded during bacterial expression. This method eliminates downstream processing steps such as oxidative refolding or cleavage of a fusion-carrier and therefore enables efficient production of insecticidal Bj-xtrIT. Periplasmic chaperone activity may produce native folding of other extensively disulfide-reticulated proteins including animal neurotoxins. This work is therefore relevant to venomics and studies of a wide range of channels and receptors
Distribution function approach to redshift space distortions. Part IV: perturbation theory applied to dark matter
We develop a perturbative approach to redshift space distortions (RSD) using
the phase space distribution function approach and apply it to the dark matter
redshift space power spectrum and its moments. RSD can be written as a sum over
density weighted velocity moments correlators, with the lowest order being
density, momentum density and stress energy density. We use standard and
extended perturbation theory (PT) to determine their auto and cross
correlators, comparing them to N-body simulations. We show which of the terms
can be modeled well with the standard PT and which need additional terms that
include higher order corrections which cannot be modeled in PT. Most of these
additional terms are related to the small scale velocity dispersion effects,
the so called finger of god (FoG) effects, which affect some, but not all, of
the terms in this expansion, and which can be approximately modeled using a
simple physically motivated ansatz such as the halo model. We point out that
there are several velocity dispersions that enter into the detailed RSD
analysis with very different amplitudes, which can be approximately predicted
by the halo model. In contrast to previous models our approach systematically
includes all of the terms at a given order in PT and provides a physical
interpretation for the small scale dispersion values. We investigate RSD power
spectrum as a function of \mu, the cosine of the angle between the Fourier mode
and line of sight, focusing on the lowest order powers of \mu and multipole
moments which dominate the observable RSD power spectrum. Overall we find
considerable success in modeling many, but not all, of the terms in this
expansion.Comment: 37 pages, 13 figures, published in JCA
Spin polarization and magneto-luminescence of confined electron-hole systems
A BCS-like variational wave-function, which is exact in the infinite field
limit, is used to study the interplay among Zeeman energies, lateral
confinement and particle correlations induced by the Coulomb interactions in
strongly pumped neutral quantum dots. Band mixing effects are partially
incorporated by means of field-dependent masses and g-factors. The spin
polarization and the magneto-luminescence are computed as functions of the
number of electron-hole pairs present in the dot and the applied magnetic
field.Comment: To appear in Phys. Rev.
Problematising parent–professional partnerships in education
The value of, and need for, parent–professional partnerships is an unchallenged mantra within policy relating to ‘special educational needs’. In spite of this, partnerships continue to be experienced as problematic by both parents and professionals. This paper brings together the different perspectives of two disability researchers: one a parent of a disabled child while the other was a teacher for 20 years of children with the label autism. The paper deconstructs the concept of partnership and then, drawing on the expertise of parents, suggests how enabling and empowering parent–professional relationships might be achieved
Problematising parent–professional partnerships in education
The value of, and need for, parent–professional partnerships is an unchallenged mantra within policy relating to ‘special educational needs’. In spite of this, partnerships continue to be experienced as problematic by both parents and professionals. This paper brings together the different perspectives of two disability researchers: one a parent of a disabled child while the other was a teacher for 20 years of children with the label autism. The paper deconstructs the concept of partnership and then, drawing on the expertise of parents, suggests how enabling and empowering parent–professional relationships might be achieved
Strangeness Enhancement in p-A Collisions: Consequences for the Interpretation of Strangeness Production in A-A Collisions
Published measurements of semi-inclusive Lambda production in p-Au collisions
at the AGS are used to estimate the yields of singly strange hadrons in
nucleus-nucleus A-A collisions. Results of a described extrapolation technique
are shown and compared to measurements of K+ production in Si-Al, Si-Au, and
Au-Au collisions at the AGS and net Lambda production in Su-Su, S-Ag, Pb-Pb,
and inclusive p-A collisions at the SPS. The extrapolations can account for
more than 75% of the measured strange particle yields in all of the studied
systems except for very central Au-Au collisions at the AGS where RQMD
comparisons suggest large re-scattering contributions.Comment: 9 pages, 4 figure
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