7,586 research outputs found
Novel Au–SiO2–WO3 Core–Shell Composite Nanoparticles for Surface-Enhanced Raman Spectroscopy with Potential Application in Cancer Cell Imaging
This is the final version. Available on open access from Wiley via the DOI in this recordWith the rapid development of nanotechnology during the last decades, the ability to detect
and control individual objects at the nanoscale has enabled to deal with complex biomedical
challenges. In cancer imaging, novel nanoparticles (NPs) offer promising potential to identify
single cancer cells and precisely label larger areas of cancer tissues. Herein, a new class of
size tunable core-shell composite (Au-SiO2-WO3) nanoparticles is reported. These
nanoparticles display an easily improvable ∼ 103
surface-enhanced Raman scattering (SERS)
enhancement factor (EF) with a double Au shell for dried samples over Si wafers and several
orders of magnitude for liquid samples. WO3 core nanoparticles of 20-50 nm in diameter are
sheathed by an intermediate 10-60 nm silica layer, produced by following the Stöber basedprocess and Turkevich method, followed by a 5-20 nm thick Au outer shell. By attaching 4-
mercaptobenzoic acid (4-MBA) molecules as Raman reporters to the Au, high-resolution
Raman maps which pinpoint the nanoparticles’ location are obtained. Our preliminary results
confirm their advantageous SERS properties for single-molecule detection, significant cell
viability after 24 h and in vitro cell imaging using coherent anti-stokes Raman scattering
(CARS). Our long-term objective is to measure SERS nanoparticles in vivo using NearInfrared light.Engineering and Physical Sciences Research Council (EPSRC
Quenching of Weak Interactions in Nucleon Matter
We have calculated the one-body Fermi and Gamow-Teller charge-current, and
vector and axial-vector neutral-current nuclear matrix elements in nucleon
matter at densities of 0.08, 0.16 and 0.24 fm and proton fractions
ranging from 0.2 to 0.5. The correlated states for nucleon matter are obtained
by operating on Fermi-gas states by a symmetrized product of pair correlation
operators determined from variational calculations with the Argonne v18 and
Urbana IX two- and three-nucleon interactions. The squares of the charge
current matrix elements are found to be quenched by 20 to 25 % by the
short-range correlations in nucleon matter. Most of the quenching is due to
spin-isospin correlations induced by the pion exchange interactions which
change the isospins and spins of the nucleons. A large part of it can be
related to the probability for a spin up proton quasi-particle to be a bare
spin up/down proton/neutron. We also calculate the matrix elements of the
nuclear Hamiltonian in the same correlated basis. These provide relatively mild
effective interactions which give the variational energies in the Hartree-Fock
approximation. The calculated two-nucleon effective interaction describes the
spin-isospin susceptibilities of nuclear and neutron matter fairly accurately.
However 3-body terms are necessary to reproduce the compressibility. All
presented results use the simple 2-body cluster approximation to calculate the
correlated basis matrix elements.Comment: submitted to PR
Susceptibility and dilution effects of the kagome bi-layer geometrically frustrated network. A Ga-NMR study of SrCr_(9p)Ga_(12-9p)O_(19)
We present an extensive gallium NMR study of the geometrically frustrated
kagome bi-layer compound SrCr_(9p)Ga_(12-9p)O_(19) (Cr^3+, S=3/2) over a broad
Cr-concentration range (.72<p<.95). This allows us to probe locally the kagome
bi-layer susceptibility and separate the intrinsic properties due to the
geometric frustration from those related to the site dilution. Our major
findings are: 1) The intrinsic kagome bi-layer susceptibility exhibits a
maximum in temperature at 40-50 K and is robust to a dilution as high as ~20%.
The maximum reveals the development of short range antiferromagnetic
correlations; 2) At low-T, a highly dynamical state induces a strong wipe-out
of the NMR intensity, regardless of dilution; 3) The low-T upturn observed in
the macroscopic susceptibility is associated to paramagnetic defects which stem
from the dilution of the kagome bi-layer. The low-T analysis of the NMR
lineshape suggests that the defect can be associated with a staggered
spin-response to the vacancies on the kagome bi-layer. This, altogether with
the maximum in the kagome bi-layer susceptibility, is very similar to what is
observed in most low-dimensional antiferromagnetic correlated systems; 4) The
spin glass-like freezing observed at T_g=2-4 K is not driven by the
dilution-induced defects.Comment: 19 pages, 19 figures, revised version resubmitted to PRB Minor
modifications: Fig.11 and discussion in Sec.V on the NMR shif
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Working sick and out of sorts: a cross-cultural approach on presenteeism climate, organizational justice and work-family conflict
A climate of presenteeism has important effects on employee well-being and the organization itself. Our study, based on surveys of health sector employees in six different countries (Brazil, Ecuador, Lebanon, Portugal, Russia and Spain) examines whether organizational justice plays a mediating role in the relationship between a presenteeism climate in the organization and work-family conflict (WFC). Our results indicate that the perception of organizational justice and the presenteeism climate do influence WFC. Moreover, higher levels of WFC were found in non-Latin countries. This study contributes to the work attendance and life balance field by providing cross-cultural empirical evidence corroborating the effect of justice and presenteeism climate on the WFC
Cytogenetic analysis of three sea catfish species (Teleostei, Siluriformes, Ariidae) with the first report of Ag-NOR in this fish family
Despite their ecological and economical importance, fishes of the family Ariidae are still genetically and cytogenetically poorly studied. Among the 133 known species of ariids, only eight have been karyotyped. Cytogenetic analyses performed on Genidens barbus and Sciades herzbergii revealed that both species have 2n = 56 chromosomes and Cathorops aff. mapale has 2n = 52 chromosomes: Genidens barbus has 10 Metacentrics (M), 14 Submetacentrics (SM), 26 Subtelocentrics (ST), and 6 Acrocentrics (A), Sciades herzbergii has 14M, 20SM, 18ST and 4A, whereas Cathorops aff. mapale has 14M, 20SM, and 18ST. The nucleolus organizer regions (NORs) were found in a single chromosome pair on the short arm of a large-sized ST pair in Genidens barbus and on the short arm of a middle-size SM pair in Cathorops aff. mapale. Multiple NORs on the short arms of two large-sized ST pairs were found in Sciades herzbergii. The occurrence of diploid numbers ranging from 2n = 52 through 56 chromosomes and the presence of different karyotypic compositions, besides the number and position of NORs suggest that several numeric and structural chromosome rearrangements were fixed during the evolutionary history of this fish family
The microRNA-29 family in cartilage homeostasis and osteoarthritis
MicroRNAs have been shown to function in cartilage development and homeostasis, as well as in progression of osteoarthritis. The objective of the current study was to identify microRNAs involved in the onset or early progression of osteoarthritis and characterise their function in chondrocytes. MicroRNA expression in mouse knee joints post-DMM surgery was measured over 7 days. Expression of miR-29b-3p was increased at day 1 and regulated in the opposite direction to its potential targets. In a mouse model of cartilage injury and in end-stage human OA cartilage, the miR-29 family were also regulated. SOX9 repressed expression of miR-29a-3p and miR-29b-3p via the 29a/b1 promoter. TGFβ1 decreased expression of miR-29a, b and c (3p) in primary chondrocytes, whilst IL-1β increased (but LPS decreased) their expression. The miR-29 family negatively regulated Smad, NFκB and canonical WNT signalling pathways. Expression profiles revealed regulation of new WNT-related genes. Amongst these, FZD3, FZD5, DVL3, FRAT2, CK2A2 were validated as direct targets of the miR-29 family. These data identify the miR-29 family as microRNAs acting across development and progression of OA. They are regulated by factors which are important in OA and impact on relevant signalling pathways
Operational experience, improvements, and performance of the CDF Run II silicon vertex detector
The Collider Detector at Fermilab (CDF) pursues a broad physics program at
Fermilab's Tevatron collider. Between Run II commissioning in early 2001 and
the end of operations in September 2011, the Tevatron delivered 12 fb-1 of
integrated luminosity of p-pbar collisions at sqrt(s)=1.96 TeV. Many physics
analyses undertaken by CDF require heavy flavor tagging with large charged
particle tracking acceptance. To realize these goals, in 2001 CDF installed
eight layers of silicon microstrip detectors around its interaction region.
These detectors were designed for 2--5 years of operation, radiation doses up
to 2 Mrad (0.02 Gy), and were expected to be replaced in 2004. The sensors were
not replaced, and the Tevatron run was extended for several years beyond its
design, exposing the sensors and electronics to much higher radiation doses
than anticipated. In this paper we describe the operational challenges
encountered over the past 10 years of running the CDF silicon detectors, the
preventive measures undertaken, and the improvements made along the way to
ensure their optimal performance for collecting high quality physics data. In
addition, we describe the quantities and methods used to monitor radiation
damage in the sensors for optimal performance and summarize the detector
performance quantities important to CDF's physics program, including vertex
resolution, heavy flavor tagging, and silicon vertex trigger performance.Comment: Preprint accepted for publication in Nuclear Instruments and Methods
A (07/31/2013
Association between footwear use and neglected tropical diseases: a systematic review and meta-analysis
BACKGROUND
The control of neglected tropical diseases (NTDs) has primarily focused on preventive chemotherapy and case management. Less attention has been placed on the role of ensuring access to adequate water, sanitation, and hygiene and personal preventive measures in reducing exposure to infection. Our aim was to assess whether footwear use was associated with a lower risk of selected NTDs.
METHODOLOGY
We conducted a systematic review and meta-analysis to assess the association between footwear use and infection or disease for those NTDs for which the route of transmission or occurrence may be through the feet. We included Buruli ulcer, cutaneous larva migrans (CLM), leptospirosis, mycetoma, myiasis, podoconiosis, snakebite, tungiasis, and soil-transmitted helminth (STH) infections, particularly hookworm infection and strongyloidiasis. We searched Medline, Embase, Cochrane, Web of Science, CINAHL Plus, and Popline databases, contacted experts, and hand-searched reference lists for eligible studies. The search was conducted in English without language, publication status, or date restrictions up to January 2014. Studies were eligible for inclusion if they reported a measure of the association between footwear use and the risk of each NTD. Publication bias was assessed using funnel plots. Descriptive study characteristics and methodological quality of the included studies were summarized. For each study outcome, both outcome and exposure data were abstracted and crude and adjusted effect estimates presented. Individual and summary odds ratio (OR) estimates and corresponding 95% confidence intervals (CIs) were calculated as a measure of intervention effect, using random effects meta-analyses.
PRINCIPAL FINDINGS
Among the 427 studies screened, 53 met our inclusion criteria. Footwear use was significantly associated with a lower odds of infection of Buruli ulcer (OR=0.15; 95% CI: 0.08-0.29), CLM (OR=0.24; 95% CI: 0.06-0.96), tungiasis (OR=0.42; 95% CI: 0.26-0.70), hookworm infection (OR=0.48; 95% CI: 0.37-0.61), any STH infection (OR=0.57; 95% CI: 0.39-0.84), strongyloidiasis (OR=0.56; 95% CI: 0.38-0.83), and leptospirosis (OR=0.59; 95% CI: 0.37-0.94). No significant association between footwear use and podoconiosis (OR=0.63; 95% CI: 0.38-1.05) was found and no data were available for mycetoma, myiasis, and snakebite. The main limitations were evidence of heterogeneity and poor study quality inherent to the observational studies included.
CONCLUSIONS/SIGNIFICANCE
Our results show that footwear use was associated with a lower odds of several different NTDs. Access to footwear should be prioritized alongside existing NTD interventions to ensure a lasting reduction of multiple NTDs and to accelerate their control and elimination.
PROTOCOL REGISTRATION
PROSPERO International prospective register of systematic reviews CRD42012003338
Guidelines for delineation of lymphatic clinical target volumes for high conformal radiotherapy: head and neck region
The success of radiotherapy depends on the accurate delineation of the clinical target volume. The delineation of the lymph node regions has most impact, especially for tumors in the head and neck region. The purpose of this article was the development an atlas for the delineation of the clinical target volume for patients, who should receive radiotherapy for a tumor of the head and neck region. Literature was reviewed for localisations of the adjacent lymph node regions and their lymph drain in dependence of the tumor entity. On this basis the lymph node regions were contoured on transversal CT slices. The probability for involvement was reviewed and a recommendation for the delineation of the CTV was generated
Spontaneous Inter-layer Coherence in Double-Layer Quantum-Hall Systems I: Charged Vortices and Kosterlitz-Thouless Phase Transitions
At strong magnetic fields double-layer two-dimensional-electron-gas systems
can form an unusual broken symmetry state with spontaneous inter-layer phase
coherence. In this paper we explore the rich variety of quantum and
finite-temperature phase transitions associated with this broken symmetry. We
describe the system using a pseudospin language in which the layer
degree-of-freedom is mapped to a fictional spin 1/2 degree-of-freedom. With
this mapping the spontaneous symmetry breaking is equivalent to that of a spin
1/2 easy-plane ferromagnet. In this language spin-textures can carry a charge.
In particular, vortices carry e/2 electrical charge and vortex-antivortex pairs
can be neutral or carry charge e. We derive an effective low-energy action and
use it to discuss the charged and collective neutral excitations of the system.
We have obtained the parameters of the Landau-Ginzburg functional from
first-principles estimates and from finite-size exact diagonalization studies.
We use these results to estimate the dependence of the critical temperature for
the Kosterlitz-Thouless phase transition on layer separation.Comment: 56 pages, 19 figures available upon request at
[email protected]. RevTex 3.0. IUCM94-00
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