Identification of Epstein-Barr virus replication proteins in Burkitt’s lymphoma cells

The working model to describe the mechanisms used to replicate the cancer-associated virus Epstein-Barr virus (EBV) is partly derived from comparisons with other members of the Herpes virus family. Many genes within the EBV genome are homologous across the herpes virus family. Published transcriptome data for the EBV genome during its lytic replication cycle show extensive transcription, but the identification of the proteins is limited. We have taken a global proteomics approach to identify viral proteins that are expressed during the EBV lytic replication cycle. We combined an enrichment method to isolate cells undergoing EBV lytic replication with SILAC-labeling coupled to mass-spectrometry and identified viral and host proteins expressed during the OPEN ACCESS Pathogens 2015, 4 740 EBV lytic replication cycle. Amongst the most frequently identified viral proteins are two components of the DNA replication machinery, the single strand DNA binding protein BALF2, DNA polymerase accessory protein BMRF1 and both subunits of the viral ribonucleoside-diphosphate reductase enzyme (BORF2 and BaRF1). An additional 42 EBV lytic cycle proteins were also detected. This provides proteomic identification for many EBV lytic replication cycle proteins and also identifies post-translational modifications

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Global and Regional Sea Level Rise Scenarios for the United States

The Sea Level Rise and Coastal Flood Hazard Scenarios and Tools Interagency Task Force, jointly convened by the U.S. Global Change Research Program (USGCRP) and the National Ocean Council (NOC), began its work in August 2015. The Task Force has focused its efforts on three primary tasks: 1) updating scenarios of global mean sea level (GMSL) rise, 2) integrating the global scenarios with regional factors contributing to sea level change for the entire U.S. coastline, and 3) incorporating these regionally appropriate scenarios within coastal risk management tools and capabilities deployed by individual agencies in support of the needs of specific stakeholder groups and user communities. This technical report focuses on the first two of these tasks and reports on the production of gridded relative sea level (RSL, which includes both ocean-level change and vertical land motion) projections for the United States associated with an updated set of GMSL scenarios. In addition to supporting the longer-term Task Force effort, this new product will be an important input into the USGCRP Sustained Assessment process and upcoming Fourth National Climate Assessment (NCA4) due in 2018. This report also serves as a key technical input into the in-progress USGCRP Climate Science Special Report (CSSR)

The RCK1 domain of the human BK_(Ca) channel transduces Ca^(2+) binding into structural rearrangements

Large-conductance voltage- and Ca^(2+)-activated K^+ (BK_(Ca)) channels play a fundamental role in cellular function by integrating information from their voltage and Ca2+ sensors to control membrane potential and Ca^(2+) homeostasis. The molecular mechanism of Ca^(2+)-dependent regulation of BKCa channels is unknown, but likely relies on the operation of two cytosolic domains, regulator of K^+ conductance (RCK)1 and RCK2. Using solution-based investigations, we demonstrate that the purified BK_(Ca) RCK1 domain adopts an α/β fold, binds Ca^(2+), and assembles into an octameric superstructure similar to prokaryotic RCK domains. Results from steady-state and time-resolved spectroscopy reveal Ca^(2+)-induced conformational changes in physiologically relevant [Ca^(2+)]. The neutralization of residues known to be involved in high-affinity Ca^(2+) sensing (D362 and D367) prevented Ca^(2+)-induced structural transitions in RCK1 but did not abolish Ca^(2+) binding. We provide evidence that the RCK1 domain is a high-affinity Ca^(2+) sensor that transduces Ca^(2+) binding into structural rearrangements, likely representing elementary steps in the Ca^(2+)-dependent activation of human BK_(Ca) channels

Community as an Institutional Learning Goal at the Unversity of Dayton

This working paper summarizes the work of the Habits of Inquiry and Reflection Community Fellows. It considers the meaning of community both in UD’s historic mission and in the ways it is practiced at UD now; identifies obstacles and failures; and offers recommendations for advancing community as a learning goal at UD

The Neutral Hydrogen Properties of Galaxies in Gas-rich Groups

We present an analysis of the integrated neutral hydrogen (Hi) properties for 27 galaxies within nine low mass, gas-rich, late-type dominated groups which we denote \Choirs". We find that majority of the central Choir galaxies have average Hi content: they have a normal gas-mass fraction with respect to isolated galaxies of the same stellar mass. In contrast, we find more satellite galaxies with a lower gas-mass fraction than isolated galaxies of the same stellar mass. A likely reason for the lower gas content in these galaxies is tidal stripping. Both the specific star formation rate and the star formation efficiency of the central group galaxies are similar to galaxies in isolation. The Choir satellite galaxies have similar specific star formation rate as galaxies in isolation, therefore satellites that exhibit a higher star formation efficiency simply owe it to their lower gas-mass fractions. We find that the most Hi massive galaxies have the largest Hi discs and fall neatly onto the Hi size-mass relation, while outliers are galaxies that are experiencing interactions. We find that high specific angular momentum could be a reason for galaxies to retain the large fraction of Hi gas in their discs. This shows that for the Choir groups with no evidence of interactions, as well as those with traces of minor mergers, the internal galaxy properties dominate over the effects of residing in a group. The probed galaxy properties strengthen evidence that the Choir groups represent the early stages of group assembly