Chesnut-Miller-Manning Papers - Accession 771

The Chesnut-Miller-Manning Family Papers consist of financial and property records, correspondence, estate records, legal documents, and other items. Included are the papers of John Chesnut (1743-1818), James Chesnut, Sr. (1773-1866), James Chesnut, Jr. (1815-1885), Stephen Decatur Miller (1787-1838), and John Laurence Manning (1816-1889). Papers pertain to the business, financial, and plantation affairs and political activities of three generations of the Chesnut family, John Chesnut, James Chesnut, Sr. and James Chesnut, Jr., as well as James Chesnut, Jr.’s father-in-law Stephen Decatur Miller and John Laurence Manning.https://digitalcommons.winthrop.edu/manuscriptcollection_findingaids/1760/thumbnail.jp

Aspirin Use in Children for Fever or Viral Syndromes

Aspirin should not be used to treat acute febrile viral illness in children. (Strength of Recommendation [SOR]: C, based on case- control studies). Although no causal link has been proven, data from case-control and historic cohort studies demonstrate an association between aspirin use and Reye syndrome

Corporate Legal Guardianship: An Innovative Concept in Advocacy and Protective Services

An article written by Michael Seelig and Sandra R. Chestnut and published in the May 1986 issue of Social Work, pages 221-223

Available Coal Resources of the Booneville 7.5–Minute Quadrangle, Owsley County, Kentucky

The Booneville Quadrangle lies within the Southwestern Reserve District of the Eastern Kentucky Coal Field. Six coal beds in the quadrangle have been commercially developed, mainly by surface mining methods, and comprise the basis of this Coal Availability Study. These beds are, in descending stratigraphic order, Copland, Whitesburg, Amburgy, Upper Elkhorn No. 3, Jellico and Manchester. A computerized Geographic Information System (GRASS) was used to calculate estimates of original, mined-out and remaining resources, restrictions to mining and available resources

Calcitonin receptor N-glycosylation enhances peptide hormone affinity by controlling receptor dynamics

The class B G protein-coupled receptor (GPCR) calcitonin receptor (CTR) is a drug target for osteoporosis and diabetes. N-glycosylation of asparagine 130 in its extracellular domain (ECD) enhances calcitonin hormone affinity with the proximal GlcNAc residue mediating this effect through an unknown mechanism. Here, we present two crystal structures of salmon calcitonin-bound, GlcNAc-bearing CTR ECD at 1.78 and 2.85 Å resolutions and analyze the mechanism of the glycan effect. The N130 GlcNAc does not contact the hormone. Surprisingly, the structures are nearly identical to a structure of hormone-bound, N-glycan-free ECD, which suggested that the GlcNAc might affect CTR dynamics not observed in the static crystallographic snapshots. Hydrogen-deuterium exchange mass spectrometry and molecular dynamics simulations revealed that glycosylation stabilized a β-sheet adjacent to the N130 GlcNAc and the N-terminal α-helix near the peptide-binding site, while increasing flexibility of the peptide-binding site turret loop. These changes due to N-glycosylation increased the ligand on-rate and decreased its off rate. The glycan effect extended to RAMP-CTR amylin receptor complexes and was also conserved in the related CGRP receptor. These results reveal that N-glycosylation can modulate GPCR function by altering receptor dynamics

Barcoding cells using cell-surface programmable DNA-binding domains

We report an approach to barcode cells through cell-surface expression of programmable zinc-finger DNA-binding domains (surface zinc fingers, sZFs). We show that sZFs enable sequence-specific labeling of living cells by dsDNA, and we develop a sequential labeling approach to image more than three cell types in mixed populations using three fluorophores. We demonstrate the versatility of sZFs through applications in which they serve as surrogate reporters, function as selective cell capture reagents and facilitate targeted cellular delivery of viruses