Appendix: Brief Chronology of Cendrars. Bibliography of Cendrars\u27s works in English

Appendix: Brief Chronology of Cendrars. Bibliography of Cendrars\u27s works in Englis

Osmotic stress sensing in Populus: Components identification of a phosphorelay system

AbstractTo study the Populus response to an osmotic stress, we have isolated one cDNA encoding a histidine-aspartate kinase (HK1) and four cDNAs encoding histidine-containing phosphotransfer proteins (HPts), HPt1–4. The predicted HK1 protein shares a typical structure with ATHK1 and SLN1 osmosensors. The 4 HPTs are characterized by the histidine phosphotransfer domain. We have shown that HK1 is upregulated during an osmotic stress in hydroponic culture. We have detected an interaction between HK1 and HPt2, using the yeast two-hybrid system. These results suggest the existence of a multi-step phosphorelay pathway probably involved in osmotic stress sensing in Populus

Highlighting type A RRs as potential regulators of the dkHK1 multi-step phosphorelay pathway in Populus

In previous studies, we highlighted a multistep phosphorelay (MSP) system in poplars composed of two hybrid-type Histidine aspartate Kinases, dkHK1a and dkHK1b, which interact with three Histidine Phosphotransfer proteins, dkHPt2, 7, and 9, which in turn interact with six type B Response Regulators. These interactions correspond to the dkHK1a-b/dkHPts/dkRRBs MSP. This MSP is putatively involved in an osmosensing pathway, as dkHK1a-b are orthologous to the Arabidopsis osmosensor AHK1, and able to complement a mutant yeast deleted for its osmosensors. Since type A RRs have been characterized as negative regulators in cytokinin MSP signaling due to their interaction with HPt proteins, we decided in this study to characterize poplar type A RRs and their implication in the MSP. For a global view of this MSP, we isolated 10 poplar type A RR cDNAs, and determined their subcellular localization to check the in silico prediction experimentally. For most of them, the in planta subcellular localization was as predicted, except for three RRAs, for which this experimental approach gave a more precise localization. Interaction studies using yeast two-hybrid and in planta BiFC assays, together with transcript expression analysis in poplar organs led to eight dkRRAs being singled out as partners which could interfere the dkHK1a-b/dkHPts/dkRRBs MSP identified in previous studies. Consequently, the results obtained in this study now provide an exhaustive view of dkHK1a-b partners belonging to a poplar MSP

Diversity and Evolution of Sensor Histidine Kinases in Eukaryotes

Histidine kinases (HKs) are primary sensor proteins that act in cell signaling pathways generically referred to as "two component systems" (TCSs). TCSs are among the most widely distributed transduction systems used by both prokaryotic and eukaryotic organisms to detect and respond to a broad range of environmental cues. The structure and distribution of HK proteins are now well documented in prokaryotes but information is still fragmentary for eukaryotes. Here, we have taken advantage of recent genomic resources to explore the structural diversity and the phylogenetic distribution of HKs in the prominent eukaryotic supergroups. Searches of the genomes of 67 eukaryotic species spread evenly throughout the phylogenetic tree of life identified 748 predicted HK proteins. Independent phylogenetic analyses of predicted HK proteins were carried out for each of the major eukaryotic supergroups. This allowed most of the compiled sequences to be categorised into previously described HK groups. Beyond the phylogenetic analysis of eukaryotic HKs, this study revealed some interesting findings: (i) characterisation of some previously undescribed eukaryotic HK groups with predicted functions putatively related to physiological traits; (ii) discovery of HK groups that were previously believed to be restricted to a single kingdom in additional supergroups and (iii) indications that some evolutionary paths have led to the appearance, transfer, duplication, and loss of HK genes in some phylogenetic lineages. This study provides an unprecedented overview of the structure and distribution of HKs in the Eukaryota and represents a first step towards deciphering the evolution of TCS signaling in living organisms

Introduction. Blaise Cendrars: A «symphonie contrastante.»

Introduction for this special issue on Blaise Cendrars

Introduction. Blaise Cendrars: A «symphonie contrastante.»

Introduction for this special issue on Blaise Cendrars

Introduction. Blaise Cendrars: A «symphonie contrastante.»

Introduction for this special issue on Blaise Cendrars

Etude d'un canal d'irrigation MIMO

Treball de recerca fet per l'alumne Nicolas Chefdor durant la seva estada al laboratori de recerca VIS-ESAII a la UPC. Dirigit pels Dr. Yolanda Bolea i Dr. Antoni Grau. Estada del 1/06/2011-30/09/2011Preprin

MIMO LPV state-space identification of an open-flow irrigation canal for control

In this paper a linear parameter varying (LPV) statespace canal control model is obtained by identification in a local way. This LPV identification procedure is based on subspace methods for some operating points of an irrigation canal covering all the operation range. Different subspace algorithms have been used and compared. The model that best represents the canal behavior in a precise way is chosen. This model has been validated by error functions and analysis correlation of residuals in a laboratory multi-reach pilot canal providing satisfactory results.Postprint (author’s final draft

Recherche d un phosphorelais multiple impliqué dans la perception et la transduction du signal stress hydrique chez le peuplier

A partir d une banque d ADNc de racines de peuplier Dorskamp , nous avons isolé un ADNc codant pour une histidine-aspartate kinase appelé HK1 et quatre ADNc codant pour trois protéines à domaine transmetteur de phosphate à histidine, HPt1, HPt2 et HPt3/4. Nous avons montré dans les racines de peuplier cultivé en hydroponie que le taux de transcrits HK1 augmente 5 min après l application d une contrainte hyper-osmotique et qu il existe une interaction entre HK1 et HPt2 chez la levure. L ensemble de ces résultats est un argument fort en faveur de l implication d un système de phosphorelais multiple dans la perception et la transduction du signal stress hydrique chez le peuplier.ORLEANS-BU Sciences (452342104) / SudocSudocFranceF