The effects of female sex hormones (birth control contraceptive) on measures of endothelial function

Background: Endothelial function has been shown to be influenced by many variables, including, but not limited to body composition, disease state, dietary fat intake, medication, and sex hormones, in particular estrogen. Specifically in women, changes in the functioning level of endothelial cells vary in response to changes in estrogen levels in the body. Purpose: The purpose of this study was to investigate the effects of hormonal birth control contraceptives on endothelial function, or more specifically, on flow-mediated dilatation (FMD). Method: 61 female participants between the ages of 18 and 28 (21.54 ± 2.03) with BMI ranging from 17.47 to 35.03 (23.04 ± 3.37) were recruited for this study from Texas Christian University by word of mouth and flier. Upon enrollment into the study, participants completed a medical questionnaire and signed a University approved informed consent. Based on self-report, the group was divided into those using birth control contraceptives (BC, n = 31) and those not using contraceptives (NC, n = 30). Endothelial function was assessed via FMD of the brachial artery using an Acuson, Aspin Advance color Doppler ultrasound unit with a L10 linear array transducer. Briefly, with the subjects in a supine position, the diameter of the brachial artery was measured in longitudinal section image using the caliper system in the Acuson ultrasound unit. The brachial artery was then occluded for 5 min with a blood pressure cuff inflated to 50 mmHg above systolic blood pressure. For 5 min following the occlusion, an image of the blood vessel diameter was obtained every 30 sec. Results: The average pre-occlusion and peak FMD responses for the groups were 0.295 ± 0.034 and 0.351 ± 0.034 cm, and 0.308 ± 0.039 and 0.373 ± 0.044 cm for the BC and NC groups, respectively. This corresponded to an average FMD percent change of 19.27 ± 9.27 and 21.52 ± 7.02 for the BC and NC groups, respectively. No significant difference was found between the two groups for either absolute or percent change responses. Conclusion: Birth control medications do not influence endothelial function assessed via flow-mediated dilatation

SCOPmap: Automated assignment of protein structures to evolutionary superfamilies

BACKGROUND: Inference of remote homology between proteins is very challenging and remains a prerogative of an expert. Thus a significant drawback to the use of evolutionary-based protein structure classifications is the difficulty in assigning new proteins to unique positions in the classification scheme with automatic methods. To address this issue, we have developed an algorithm to map protein domains to an existing structural classification scheme and have applied it to the SCOP database. RESULTS: The general strategy employed by this algorithm is to combine the results of several existing sequence and structure comparison tools applied to a query protein of known structure in order to find the homologs already classified in SCOP database and thus determine classification assignments. The algorithm is able to map domains within newly solved structures to the appropriate SCOP superfamily level with ~95% accuracy. Examples of correctly mapped remote homologs are discussed. The algorithm is also capable of identifying potential evolutionary relationships not specified in the SCOP database, thus helping to make it better. The strategy of the mapping algorithm is not limited to SCOP and can be applied to any other evolutionary-based classification scheme as well. SCOPmap is available for download. CONCLUSION: The SCOPmap program is useful for assigning domains in newly solved structures to appropriate superfamilies and for identifying evolutionary links between different superfamilies

Probabilistic Clustering of Sequences: Inferring new bacterial regulons by comparative genomics

Genome wide comparisons between enteric bacteria yield large sets of conserved putative regulatory sites on a gene by gene basis that need to be clustered into regulons. Using the assumption that regulatory sites can be represented as samples from weight matrices we derive a unique probability distribution for assignments of sites into clusters. Our algorithm, 'PROCSE' (probabilistic clustering of sequences), uses Monte-Carlo sampling of this distribution to partition and align thousands of short DNA sequences into clusters. The algorithm internally determines the number of clusters from the data, and assigns significance to the resulting clusters. We place theoretical limits on the ability of any algorithm to correctly cluster sequences drawn from weight matrices (WMs) when these WMs are unknown. Our analysis suggests that the set of all putative sites for a single genome (e.g. E. coli) is largely inadequate for clustering. When sites from different genomes are combined and all the homologous sites from the various species are used as a block, clustering becomes feasible. We predict 50-100 new regulons as well as many new members of existing regulons, potentially doubling the number of known regulatory sites in E. coli.Comment: 27 pages including 9 figures and 3 table

Vertical structure use by the Stout Iguana (Cyclura pinguis) on Guana Island, BVI

The Stout Iguana (Cyclura pinguis) is a critically endangered species endemic to the Puerto Rico Bank and currently restricted to the British Virgin Islands (BVI). Our study on Guana Island, BVI, focused on vertical structure use by adult (n = 4) and juvenile (n = 11) iguanas with tracking bobbins. We recorded structure types used, heights attained on structures, distances between structures, and structure sizes. We found that Stout Iguanas used vertical structure more than previously documented. Trees comprised a significantly greater (P <  0.001) proportion of structures used by juveniles than by adults, whereas rocks comprised the greatest proportion of structures used by adults. In addition to differential structure use, juveniles climbed significantly higher (2.4 vs. 0.9 m on average; P < 0.001) than adults. We found no difference in the diameter or distance between structures used by adults and juveniles. Our results suggest that vertical structure use may be an important habitat element for free-ranging juvenile Stout Iguanas. Habitat management that provides vertical structure may be advantageous for the conservation of this species

The State of Capacity Development Evaluation in Biodiversity Conservation and Natural Resource Management

Capacity development is critical to long-term conservation success, yet we lack a robust and rigorous understanding of how well its effects are being evaluated. A comprehensive summary of who is monitoring and evaluating capacity development interventions, what is being evaluated and how, would help in the development of evidence-based guidance to inform design and implementation decisions for future capacity development interventions and evaluations of their effectiveness. We built an evidence map by reviewing peer-reviewed and grey literature published since 2000, to identify case studies evaluating capacity development interventions in biodiversity conservation and natural resource management. We used inductive and deductive approaches to develop a coding strategy for studies that met our criteria, extracting data on the type of capacity development intervention, evaluation methods, data and analysis types, categories of outputs and outcomes assessed, and whether the study had a clear causal model and/or used a systems approach. We found that almost all studies assessed multiple outcome types: most frequent was change in knowledge, followed by behaviour, then attitude. Few studies evaluated conservation outcomes. Less than half included an explicit causal model linking interventions to expected outcomes. Half of the studies considered external factors that could influence the efficacy of the capacity development intervention, and few used an explicit systems approach. We used framework synthesis to situate our evidence map within the broader literature on capacity development evaluation. Our evidence map (including a visual heat map) highlights areas of low and high representation in investment in research on the evaluation of capacity development

Juegos serios para el tratamiento o la prevención de la depresión: una revisión sistemática

Serious games (computerised interventions which utilise gaming for serious purposes) have been shown to support improved outcomes in several health conditions. We aimed to review evidence regarding serious games for depression. We undertook electronic searches of PsycInfo, EMBASE and Medline, using terms relevant to computer games and depression. We included fulltext articles published in English in peer-reviewed literature since 2000, where the intervention was designed to treat or prevent depression and which included pre-and post-intervention measurement of depression. Nine studies relating to a total of six interventions met inclusion criteria. Most studies were small and were carried out by the developers of the programs. All were tested with young people (ages between 9 and 25 years). Most reported promising results with some positive impact on depression although one universal program had mixed results. Serious gaming interventions show promise for depression, however evidence is currently very limited.Se ha demostrado que los juegos serios (intervenciones computarizadas que utilizan juegos) mejoran los resultados en diferentes problemas de salud. Pretendemos examinar las evidencias de estos juegos para la depresión. Se realizaron búsquedas electrónicas en PsycINFO, EMBASE y Medline usando términos relacionados con juegos de ordenador y depresión. Se incluyeron artículos publicados desde el año 2000, donde se diseñó la intervención para tratar o prevenir la depresión incluyendo medidas pre- y post-intervención. Nueve estudios sobre un total de seis intervenciones cumplieron los criterios de inclusión. La mayoría de estos fueron pequeños y los llevaron a cabo los desarrolladores de los programas. Todos incluían población joven (9 - 25 años). La mayoría presentan resultados prometedores con un impacto positivo sobre la depresión aunque un programa universal tuvo resultados mixtos. Se concluye que las intervenciones basadas en juegos serios son prometedoras para la depresión, aunque la evidencia es todavía muy limitada

Radiotherapy optimAl Design: An Academic Radiotherapy Treatment Design System

Optimally designing radiotherapy and radiosurgery treatments to increase the likelihood of a successful recovery from cancer is an important application of operations research. Researchers have been hindered by the lack of academic software that supports head-to-head comparisons of different techniques, and this article addresses the inherent difficulties of designing and implementing an academic treatment planning system. In particular, this article details the algorithms and the software design of Radiotherapy optimAl Design (RAD)

Global distribution patterns of mycoheterotrophy

Environmental Biolog