The effect of an indoor-outdoor temperature difference on transient cross-ventilation

We examine the effect of an indoor-outdoor temperature difference on the transient wind-driven cross-ventilation of a room. Laboratory experiments are performed in a water flume using a reduced-scale model room. For solely wind-driven cross-ventilation with no initial temperature difference between the room and the external fluid, the ventilation rate is constant. In experiments, the mean dye concentration decays exponentially, which is expected when the room remains well-mixed. When there is an initial temperature difference but no wind, the buoyancy-driven exchange-ventilation results lie between a model that assumes the room is well-mixed and a new model that assumes no mixing between the incoming flow and the room. When both wind and buoyancy drive the flow, the relative importance of these two effects can be described by a Froude number, Fr. For buoyancy-dominated ventilation (Fr1), a temperature difference slightly reduces the ventilation rate, but only by up to 6%, a change that can be neglected in most applications. Two processes compete to ventilate the room in combined cases: the removal of fluid from a lower layer by flow through the windows and the erosion of an upper layer by entrainment into the jet that crosses the room. The relative rates of these two processes depend on the geometry of the room.This work is supported by the Engineering and Physical Sciences Research Council (EPSRC) Grand Challenge grant Managing Air for Green Inner Cities (MAGIC) [grant number EP/N010221/1]

Specific prebiotics modulate gut microbiota and immune activation in HAART-naive HIV-infected adults: results of the “COPA” pilot randomized trial

Intestinal mucosal immune system is an early target for human immunodeficiency virus type 1 (HIV-1) infection, resulting in CD4+ T-cell depletion, deterioration of gut lining, and fecal microbiota composition. We evaluated the effects of a prebiotic oligosaccharide mixture in highly active antiretroviral therapy (HAART)-naive HIV-1-infected adults. In a pilot double-blind, randomized, placebo-controlled study, 57 HAART-naive HIV-1-infected patients received a unique oligosaccharide mixture (15 or 30 g short chain galactooligosaccharides/long chain fructooligosaccharides/pectin hydrolysate-derived acidic oligosaccharides (scGOS/lcFOS/pAOS) daily) or a placebo for 12 weeks. Microbiota composition improved significantly with increased bifidobacteria, decreased Clostridium coccoides/Eubacterium rectale cluster, and decreased pathogenic Clostridium lituseburense/Clostridium histolyticum group levels upon prebiotic supplementation. In addition, a reduction of soluble CD14 (sCD14), activated CD4+/CD25+ T cells, and significantly increased natural killer (NK) cell activity when compared with control group were seen in the treatment group. The results of this pilot trial highly significantly show that dietary supplementation with a prebiotic oligosaccharide mixture results in improvement of the gut microbiota composition, reduction of sCD14, CD4+ T-cell activation (CD25), and improved NK cell activity in HAART-naive HIV-infected individuals

Infection of Semen-Producing Organs by SIV during the Acute and Chronic Stages of the Disease

International audienceBACKGROUND: Although indirect evidence suggests the male genital tract as a possible source of persistent HIV shedding in semen during antiretroviral therapy, this phenomenon is poorly understood due to the difficulty of sampling semen-producing organs in HIV+ asymptomatic individuals. METHODOLOGY/PRINCIPAL FINDINGS: Using a range of molecular and cell biological techniques, this study investigates SIV infection within reproductive organs of macaques during the acute and chronic stages of the disease. We demonstrate for the first time the presence of SIV in the testes, epididymides, prostate and seminal vesicles as early as 14 days post-inoculation. This infection persists throughout the chronic stage and positively correlates with blood viremia. The prostate and seminal vesicles appear to be the most efficiently infected reproductive organs, followed by the epididymides and testes. Within the male genital tract, mostly T lymphocytes and a small number of germ cells harbour SIV antigens and RNA. In contrast to the other organs studied, the testis does not display an immune response to the infection. Testosteronemia is transiently increased during the early phase of the infection but spermatogenesis remains unaffected. CONCLUSIONS/SIGNIFICANCE: The present study reveals that SIV infection of the macaque male genital tract is an early event and that semen-producing organs display differential infection levels and immune responses. These results help elucidate the origin of HIV in semen and constitute an essential base to improving the design of antiretroviral therapies to eradicate virus from semen

The effect of an indoor-outdoor temperature difference on transient cross-ventilation

We examine the effect of an indoor-outdoor temperature difference on the transient wind-driven cross-ventilation of a room. Laboratory experiments are performed in a water flume using a reduced-scale model room. For solely wind-driven cross-ventilation with no initial temperature difference between the room and the external fluid, the ventilation rate is constant. In experiments, the mean dye concentration decays exponentially, which is expected when the room remains well-mixed. When there is an initial temperature difference but no wind, the buoyancy-driven exchange-ventilation results lie between a model that assumes the room is well-mixed and a new model that assumes no mixing between the incoming flow and the room. When both wind and buoyancy drive the flow, the relative importance of these two effects can be described by a Froude number, Fr. For buoyancy-dominated ventilation (Fr1), a temperature difference slightly reduces the ventilation rate, but only by up to 6%, a change that can be neglected in most applications. Two processes compete to ventilate the room in combined cases: the removal of fluid from a lower layer by flow through the windows and the erosion of an upper layer by entrainment into the jet that crosses the room. The relative rates of these two processes depend on the geometry of the room

Stage-specific IFN-induced and IFN gene expression reveal convergence of type I and type II IFN and highlight their role in both acute and chronic stage of pathogenic SIV infection.

Interferons (IFNs) play a major role in controlling viral infections including HIV/SIV infections. Persistent up-regulation of interferon stimulated genes (ISGs) is associated with chronic immune activation and progression in SIV/HIV infections, but the respective contribution of different IFNs is unclear. We analyzed the expression of IFN genes and ISGs in tissues of SIV infected macaques to understand the respective roles of type I and type II IFNs. Both IFN types were induced in lymph nodes during early stage of primary infection and to some extent in rectal biopsies but not in PBMCs. Induction of Type II IFN expression persisted during the chronic phase, in contrast to undetectable induction of type I IFN expression. Global gene expression analysis with a major focus on ISGs revealed that at both acute and chronic infection phases most differentially expressed ISGs were inducible by both type I and type II IFNs and displayed the highest increases, indicating strong convergence and synergy between type I and type II IFNs. The analysis of functional signatures of ISG expression revealed temporal changes in IFN expression patterns identifying phase-specific ISGs. These results suggest that IFN-γ strongly contribute to shape ISG upregulation in addition to type I IFN