Effect of Gender, Energetics, and Biomechanics on Swimming Masters Performance

The purpose of this study was to analyze the effect of gender and energetics on biomechanics and performance of masters swimmers over 1 season. Twenty-five masters swimmers (14 male and 11 female) were assessed 3 times (TP1, TP2, and TP3) during a season (male personal record in 200-m freestyle event: 173.00 ± 31.41 seconds: female personal record in 200-m freestyle event: 200.73 ± 25.02 seconds). An incremental 5 × 200-m step test was selected to evaluate velocity at 4 mmol·l⁻¹ of blood lactate concentration (v4), maximal blood lactate concentration after exercise (La(peak)), maximal oxygen uptake (V̇O2max), stroke frequency, stroke length (SL), stroke index (SI), and propelling efficiency of the arm stroke (η(p)). The 200-m freestyle performance and average swimming velocity (v200) were also monitored. Significant differences were observed between males and females for the 200-m freestyle performance, SL, SI, and La(peak). Performance (205.18 ± 24.47 seconds; 197.45 ± 20.97 seconds; 193.45 ± 18.12 seconds), SL (1.69 ± 0.17 m; 1.79 ± 0.13 m; 1.78 ± 0.15 m), SI (1.68 ± 0.31 m²·c⁻¹·s⁻¹; 1.83 ± 0.27 m²·c⁻¹·s⁻¹; 1.85 ± 0.27 m²·c⁻¹·s⁻¹), η(p) (0.32 ± 0.04; 0.33 ± 0.03; 0.33 ± 0.04), and V̇O2max (38.71 ± 3.44 ml·kg⁻¹·min⁻¹; 43.43 ± 3.71 ml·kg⁻¹·min⁻¹; 43.95 ± 7.02 ml·kg⁻¹·min⁻¹) have changed significantly throughout the season (TP1, TP2, and TP3, respectively) in female swimmers. In male, significant changes were found in η(p) (0.33 ± 0.07; 0.36 ± 0.05; 0.36 ± 0.06) and V̇O2max (41.65 ± 7.30 ml·kg⁻¹·min⁻¹; 45.19 ± 6.55 ml·kg⁻¹·min⁻¹; 50.19 ± 9.65 ml·kg⁻¹·min⁻¹) over the season (TP1, TP2, and TP3, respectively). Gender presented a significant effect on SL (TP2: η(p)² = 0.29; TP3: η(p)² = 0.37), SI (TP2: η(p)² = 0.25), and La(peak) (TP3: η(p)² = 0.42). v4 (TP1: η(p)² = 0.23), SL (TP1: η(p)² = 0.46), SI (TP1: η(p)² = 0.78; TP2: η(p)² = 0.37; TP3: η(p)² = 0.32), and η(p) (TP1: η(p)² = 0.28) had a significant effect on performance. Male masters swimmers have better performance, SL, SI, and La(peak) than female counterparts. Female masters swimmers enhanced significantly the 200-m freestyle performance over the season due to the improvement in swimming technique (SL, SI, and η(p)) and energetic factors (v4 and V̇O2max). Nonsignificant improvements were observed for the males' performance. Gender has a significant effect on SL, SI, and La(peak). Therefore, performance is more dependent on technical factors than energetics.This work was supported by University of Beira Interior and Santander Totta bank (UBI/ FCSH/Santander/2010).info:eu-repo/semantics/publishedVersio

Aplicação de dados geofísicos na caracterização de falhas com potencial sismogénico no Vale Inferior do Tejo

O Vale Inferior do Tejo (VIT) tem sofrido os efeitos de sismos históricos. Algumas das fontes sismogénicas são conhecidas. Com vista a uma avaliação correcta das fontes sismogénicas em futuros estudos de perigosidade sísmica, integraram-se dados de reflexão sísmica, com dados de métodos potenciais e dados de epicentros relocalizados. As informações recolhidas a partir dos parâmetros das falhas irão ajudar à localização de fontes de sismos históricos utilizando elementos finitos de simulação de movimentos fortes

Seismogenic sources in the Lower Tagus Area using geophysical data

The Lisbon and Lower Tagus Valley area have suffered the effect of historical earthquakes that caused important damages and loss of lives. Some of these earthquake sources are local but they are still poorly known due to the difficulty in finding geological outcrops, together with low-slip rates and erosion/sedimentation processes that erase surface ruptures. Identification of seismogenic sources capable of producing large earthquakes from low magnitude instrumental seismicity has also proved unreliable in other areas of the world. Therefore, for a proper assessment of seismogenic sources for seismic hazard purposes we have used recently reprocessed and reinterpreted seismic reflection, potential-field data and overlaid relocated epicentres. Here we present a revised structural interpretation of the area based on seismic data and a basement map produced by magnetic modelling, 2D Euler deconvolution and trend analysis. This basement map, which greatly enlarges the seismic coverage of the study area, has shown new insights into deep-seated structures of the Lower Tagus Valley area and their relationship with seismicity. The information from fault parameters collected in this work will also help to locate the sources of historical events such as the 1909 Benavente earthquake using finite-element strong-motion simulation and comparison with observed seismic intensitie

Natural Resistance of Leishmania infantum to Miltefosine Contributes to the Low Efficacy in the Treatment of Visceral Leishmaniasis in Brazil

In India visceral leishmaniasis (VL) caused by Leishmania donovani has been successfully treated with miltefosine with a cure rate of > 90%. To assess the efficacy and safety of oral miltefosine in L. infantum-causing Brazilian VL patients, a phase II, open-label, dose-escalation study of oral miltefosine was conducted in children (ages 2-12) and adolescent-adults (ages 13-60). Definitive cure was assessed at a 6 month follow-up visit. The cure rate was only 42% (6 out of 14 patients) with the recommended 28 days of therapy and 68% (19 out of 28 patients) with an extended treatment of 42 days. The in vitro miltefosine susceptibility profile of intracellular amastigote stages of the pre-treatment isolates, from cured and relapsed patients, showed a positive correlation with clinical outcome. The IC50 mean (SEM) of eventual cures was 5.1 (0.4) µM whereas that of eventual failures was 12.8 (1.9) µM (P = 0.0002). An IC50 below or above 8.0 µM predicts cure or failure, respectively with 82% sensitivity and 100% specificity. The finding of L. infantum amastigotes resistant to miltefosine in isolates from patients who eventually failed treatment, strongly suggests natural resistance to this drug, as miltefosine had never been used in Brazil before this trial was carried out

Sensitive and specific serodiagnosis of Leishmania infantum infection in dogs by using peptides selected from hypothetical proteins identified by an immunoproteomic approach

In Brazil, the percentage of infected dogs living in areas where canine visceral leishmaniasis (CVL) is endemic ranges from 10 to 62%; however, the prevalence of infection in dogs is probably higher than figures reported from serological studies. In addition, problems with the occurrence of false-positive or false-negative results in the serodiagnosis of CVL have been reported. The present work analyzed the potential of synthetic peptides mapped from hypothetical proteins for improvement of the serodiagnosis of Leishmania infantum infection in dogs. From 26 identified leishmanial proteins, eight were selected, considering that no homologies between these proteins and others from trypanosomatide sequence databases were encountered. The sequences of these proteins were mapped to identify linear B-cell epitopes, and 17 peptides were synthesized and tested in enzyme-linked immunosorbent assays (ELISAs) for the serodiagnosis of L. infantum infection in dogs. Of these, three exhibited sensitivity and specificity values higher than 75% and 90%, respectively, to differentiate L. infantum-infected animals from Trypanosoma cruziinfected animals and healthy animals. Soluble Leishmania antigen (SLA) showed poor sensitivity (4%) and specificity (36%) to differentiate L. infantum-infected dogs from healthy and T. cruzi-infected dogs. Lastly, the three selected peptides were combined in different mixtures and higher sensitivity and specificity values were obtained, even when sera from T. cruzi-infected dogs were used. The study’s findings suggest that these three peptides can constitute a potential tool for more sensitive and specific serodiagnosis of L. infantum infection in dogsThis work was supported by grants from the Pró-Reitoria de Pesquisa from UFMG (Edital 07/2012), Instituto Nacional de Ciência e Tecnologia em Nano-biofarmacêutica (INCT-NANOBIOFAR, Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG) (CBB-APQ-02364-08, CBB-APQ-00356-10, CBB-APQ-00496-11, and CBB-APQ-00819-12), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) (APQ-472090/2011-9), and the Instituto Nacional de Ciência e Tecnologia em Vacinas (INCT-V). E.A.F.C. and A.P.F. are CNPq grant recipients. M.A.C.-F. is a FAPEMIG/CAPES grant recipient. This study was supported in Spain, in part, by grants from the Ministerio de Ciencia e Innovación (FIS/PI1100095)

Cinerascetins, new peptides from Hypsiboas cinerascens : MALDI LIFT-TOF-MS/MS de novo sequence and imaging analysis

The continuous search for antimicrobial candidates pushes the pursuit of compounds in the most diverse organisms. Amphibians are known as a prolific source of antibacterial peptides. Based on the rich biodiversity of the Amazon region the unexplored green-tree frog (Hypsiboas cinerascens) was studied for its skin secretion peptide content. Chromatographic separations and established tandem mass spectrometry (MS/MS) methods were used for sequencing the primary structures of the purified compounds. De novo sequencing lead to the identification of five new peptides related to hylaseptin P1, displaying an aminated C-terminal. Sequencing of the complementary deoxyribonucleic acid (cDNA) analysis allowed the disambiguation of isobaric amino-acids for C-01. Matrix assisted laser desorption ionization (MALDI) was carried out, demonstrating the in situ co-occurrence of the identified peptides in the dorsal skin. The major peptide C-01 was synthesized and assayed against a selection of microorganisms displaying minimal inhibitory concentrations (MICs) ranging from 4 to 16 µM

Wild dogs at stake: deforestation threatens the only Amazon endemic canid, the short-eared dog (Atelocynus microtis)

The persistent high deforestation rate and fragmentation of the Amazon forests are the main threats to their biodiversity. To anticipate and mitigate these threats, it is important to understand and predict how species respond to the rapidly changing landscape. The short-eared dog Atelocynus microtis is the only Amazon-endemic canid and one of the most understudied wild dogs worldwide. We investigated short-eared dog habitat associations on two spatial scales. First, we used the largest record database ever compiled for short-eared dogs in combination with species distribution models to map species habitat suitability, estimate its distribution range and predict shifts in species distribution in response to predicted deforestation across the entire Amazon (regional scale). Second, we used systematic camera trap surveys and occupancy models to investigate how forest cover and forest fragmentation affect the space use of this species in the Southern Brazilian Amazon (local scale). Species distribution models suggested that the short-eared dog potentially occurs over an extensive and continuous area, through most of the Amazon region south of the Amazon River. However, approximately 30% of the short-eared dog's current distribution is expected to be lost or suffer sharp declines in habitat suitability by 2027 (within three generations) due to forest loss. This proportion might reach 40% of the species distribution in unprotected areas and exceed 60% in some interfluves (i.e. portions of land separated by large rivers) of the Amazon basin. Our local-scale analysis indicated that the presence of forest positively affected short-eared dog space use, while the density of forest edges had a negative effect. Beyond shedding light on the ecology of the short-eared dog and refining its distribution range, our results stress that forest loss poses a serious threat to the conservation of the species in a short time frame. Hence, we propose a re-assessment of the short-eared dog's current IUCN Red List status (Near Threatened) based on findings presented here. Our study exemplifies how data can be integrated across sources and modelling procedures to improve our knowledge of relatively understudied species