Os livros entre as cores e a conscientização de professores para o uso da biblioteca escolar

This study aims to report the activities undertaken during the Supervised Course of Specialization in Educational Supervision, State University of Piauí (UESPI), to promote the use of the school library an educational unit of the state in the city of Teresina (PI .) In a diagnosis it was found that the school library ineffective its purposes and objectives. The motivation for this, according to the speeches made by the manager and supervisor is the lack of a professional within the school library. Our interventionist project was developed in order to demystify this concept from the awareness of teachers and the entire teaching staff of the school based activities that enable a reflection on the importance of this area and especially from the organization by classification in color permits the organization and usability of the acquis in a practical way, without necessarily having a person to enable the loan worksO presente trabalho tem por objetivo relatar as atividades desenvolvidas durante o Estágio Supervisionado do curso de Especialização em Supervisão Educacional da Universidade Estadual do Piauí (UESPI), para promover a utilização da biblioteca escolar de uma unidade educacional da rede estadual no município de Teresina (PI). Em um diagnóstico constatou-se que a biblioteca da escola não efetiva as suas finalidades e objetivos. A motivação para tal, segundo os discursos proferidos pela gestora e supervisora decorre da ausência de um profissional no espaço da biblioteca escolar. Nosso projeto interventivo foi desenvolvido com o intuito de desmitificar esta concepção a partir da conscientização dos docentes e de toda a equipe pedagógica da escola a partir de atividades que permitissem uma reflexão sobre a importância deste espaço e principalmente a partir da organização mediante a classificação em cores que possibilita a organização e usabilidade do acervo de forma prática, sem necessariamente ter uma pessoa para viabilizar o empréstimo das obras

Dia D da cardiopatia congênita como ação de extensão universitária: contribuindo com o diagnóstico precoce das cardiopatias / D-Day of Congenital Heart Disease as a University Extension Action: Contributing to the Early Diagnosis of Heart Diseases

INTRODUÇÃO: A cardiopatia congênita (CC) permanece como importante causa  de óbito por malformações congênitas, sendo a terceira causa de morte no período  neonatal. O seu diagnóstico precoce é fundamental. OBJETIVO: Descrever uma ação de  educação em saúde voltada à conscientização sobre as CC para população geral.  METODOLOGIA: Estudo descritivo e qualitativo. RESULTADOS: A ação contou com  um público alvo estimado de 800 pessoas e cerca de 100 participantes na equipe  organizadora. O evento “Dia D da CC” (DDCC) permitiu divulgar o conhecimento básico  sobre CC, a importância da rede de relações sociais no enfrentamento às dificuldades do  cotidiano da criança cardiopata, e a necessidade da presença de equipe multiprofissional.  Os acadêmicos desenvolveram habilidades importantes, em virtude do uso de estratégias  participativas, da articulação com a equipe de saúde e do estabelecimento do vínculo com a  público-alvo. O DDCC consolidou um grande processo de ensino-aprendizagem para os  acadêmicos e profissionais participantes, tendo em vista o engajamento geral para a  realização de um projeto voluntário e de cunho educativo. CONCLUSÕES: O DDCC se  constituiu em uma prática de educação em saúde, expandindo as atividades acadêmicas em prol do ensino e da realização de atividades sociais, de modo a fomentar a conscientização  acerca das CC, contribuindo para a melhora da qualidade de vida da população acometida  e com a busca pela redução da morbimortalidade

Principais fatores associados ao Diabetes Mellitus Gestacional (DMG): uma revisão

Pregnancy is a physiological process for human reproduction. However, there are some factors that hinder this gestational process, such as Gestational Diabetes Mellitus (GDM), which is defined as any degree of glucose intolerance, with the first recognition during pregnancy. This study aimed to identify the factors associated with Diabetes GDM in pregnant women. Through an integrative literature review, descriptive and exploratory based on scientific evidence. Confirming that GDM is associated with a sedentary lifestyle, poor diet and that it can trigger miscarriage and pre-eclampsia, in addition to causing problems for both mother and baby. The nursing diagnosis in prenatal care makes all the difference, for screening and early treatment, through interventions and health education. It is still of great importance that the nurse advises on the risk factors and thus contributes to the prevention of more pathologies such as GDM, which can be avoided. Keywords: Gestational Diabetes Mellitus; Pregnancy; Risk factorsEl embarazo es un proceso fisiológico de la reproducción humana. Sin embargo, existen algunos factores que dificultan este proceso gestacional, como la Diabetes Mellitus Gestacional (DMG), que se define como cualquier grado de intolerancia a la glucosa, con el primer reconocimiento durante el embarazo. Este estudio tuvo como objetivo identificar los factores asociados con la Diabetes DMG en mujeres embarazadas. A través de una revisión bibliográfica integradora, descriptiva y exploratoria basada en evidencia científica. Confirmando que la DMG está asociada al sedentarismo, a la mala alimentación y que puede desencadenar abortos y preeclampsia, además de causar problemas tanto a la madre como al bebé. El diagnóstico de enfermería en el prenatal marca la diferencia, para el tamizaje y tratamiento precoz, a través de intervenciones y educación en salud. Sigue siendo de gran importancia que la enfermera asesore sobre los factores de riesgo y así contribuir a la prevención de más patologías como la DMG, que se pueden evitar. Palabras clave: Diabetes Mellitus Gestacional; El embarazo; Factores de riesgoA gestação é um processo fisiológico para a reprodução humana. Entretanto, existem alguns fatores que dificultam esse processo gestacional como a Diabetes Mellitus Gestacional (DMG), que definhado como qualquer grau de intolerância à glicose, com o primeiro reconhecimento na gestação. Este estudo teve por finalidade identificar os fatores associados ao Diabetes DMG em gestantes. Por meio de uma revisão integrativa da literatura, de caráter descritivo e exploratório baseado em evidências científicas. Confirmando que a DMG está associada ao sedentarismo, má alimentação e que pode desencadear aborto espontâneo e pré-eclâmpsia, além de trazer problemas tanto para mãe como ao bebê. O diagnóstico da enfermagem no pré-natal faz toda diferença, para o rastreamento e tratamento precoce, por meio intervenções e educação em saúde. Ainda é de grande importância que o enfermeiro oriente quanto aos fatores de risco e assim contribua para a prevenção de mais patologias como a DMG, que podem ser evitada. &nbsp

Transcriptional profiles of the human pathogenic fungus paracoccidioides brasiliensis in mycelium and yeast cells

This work was supported by MCT, CNPq, CAPES, FUB, UFG, and FUNDECT-MS. PbGenome Network: Alda Maria T. Ferreira, Alessandra Dantas, Alessandra J. Baptista, Alexandre M. Bailão, Ana Lídia Bonato, André C. Amaral, Bruno S. Daher, Camila M. Silva, Christiane S. Costa, Clayton L. Borges, Cléber O. Soares, Cristina M. Junta, Daniel A. S. Anjos, Edans F. O. Sandes, Eduardo A. Donadi, Elza T. Sakamoto-Hojo, Flábio R. Araújo, Flávia C. Albuquerque, Gina C. Oliveira, João Ricardo M. Almeida, Juliana C. Oliveira, Kláudia G. Jorge, Larissa Fernandes, Lorena S. Derengowski, Luís Artur M. Bataus, Marcus A. M. Araújo, Marcus K. Inoue, Marlene T. De-Souza, Mauro F. Almeida, Nádia S. Parachin, Nadya S. Castro, Odair P. Martins, Patrícia L. N. Costa, Paula Sandrin-Garcia, Renata B. A. Soares, Stephano S. Mello, and Viviane C. B. ReisParacoccidioides brasiliensis is the causative agent of paracoccidioidomycosis, a disease that affects 10 million individuals in Latin America. This report depicts the results of the analysis of 6,022 assembled groups from mycelium and yeast phase expressed sequence tags, covering about 80% of the estimated genome of this dimorphic, thermo-regulated fungus. The data provide a comprehensive view of the fungal metabolism, including overexpressed transcripts, stage-specific genes, and also those that are up- or down-regulated as assessed by in silico electronic subtraction and cDNA microarrays. Also, a significant differential expression pattern in mycelium and yeast cells was detected, which was confirmed by Northern blot analysis, providing insights into differential metabolic adaptations. The overall transcriptome analysis provided information about sequences related to the cell cycle, stress response, drug resistance, and signal transduction pathways of the pathogen. Novel P. brasiliensis genes have been identified, probably corresponding to proteins that should be addressed as virulence factor candidates and potential new drug targets

Comprehensive Fragment Screening of the SARS-CoV-2 Proteome Explores Novel Chemical Space for Drug Development

12 pags., 4 figs., 3 tabs.SARS-CoV-2 (SCoV2) and its variants of concern pose serious challenges to the public health. The variants increased challenges to vaccines, thus necessitating for development of new intervention strategies including anti-virals. Within the international Covid19-NMR consortium, we have identified binders targeting the RNA genome of SCoV2. We established protocols for the production and NMR characterization of more than 80 % of all SCoV2 proteins. Here, we performed an NMR screening using a fragment library for binding to 25 SCoV2 proteins and identified hits also against previously unexplored SCoV2 proteins. Computational mapping was used to predict binding sites and identify functional moieties (chemotypes) of the ligands occupying these pockets. Striking consensus was observed between NMR-detected binding sites of the main protease and the computational procedure. Our investigation provides novel structural and chemical space for structure-based drug design against the SCoV2 proteome.Work at BMRZ is supported by the state of Hesse. Work in Covid19-NMR was supported by the Goethe Corona Funds, by the IWBEFRE-program 20007375 of state of Hesse, the DFG through CRC902: “Molecular Principles of RNA-based regulation.” and through infrastructure funds (project numbers: 277478796, 277479031, 392682309, 452632086, 70653611) and by European Union’s Horizon 2020 research and innovation program iNEXT-discovery under grant agreement No 871037. BY-COVID receives funding from the European Union’s Horizon Europe Research and Innovation Programme under grant agreement number 101046203. “INSPIRED” (MIS 5002550) project, implemented under the Action “Reinforcement of the Research and Innovation Infrastructure,” funded by the Operational Program “Competitiveness, Entrepreneurship and Innovation” (NSRF 2014–2020) and co-financed by Greece and the EU (European Regional Development Fund) and the FP7 REGPOT CT-2011-285950—“SEE-DRUG” project (purchase of UPAT’s 700 MHz NMR equipment). The support of the CERM/CIRMMP center of Instruct-ERIC is gratefully acknowledged. This work has been funded in part by a grant of the Italian Ministry of University and Research (FISR2020IP_02112, ID-COVID) and by Fondazione CR Firenze. A.S. is supported by the Deutsche Forschungsgemeinschaft [SFB902/B16, SCHL2062/2-1] and the Johanna Quandt Young Academy at Goethe [2019/AS01]. M.H. and C.F. thank SFB902 and the Stiftung Polytechnische Gesellschaft for the Scholarship. L.L. work was supported by the French National Research Agency (ANR, NMR-SCoV2-ORF8), the Fondation de la Recherche Médicale (FRM, NMR-SCoV2-ORF8), FINOVI and the IR-RMN-THC Fr3050 CNRS. Work at UConn Health was supported by grants from the US National Institutes of Health (R01 GM135592 to B.H., P41 GM111135 and R01 GM123249 to J.C.H.) and the US National Science Foundation (DBI 2030601 to J.C.H.). Latvian Council of Science Grant No. VPP-COVID-2020/1-0014. National Science Foundation EAGER MCB-2031269. This work was supported by the grant Krebsliga KFS-4903-08-2019 and SNF-311030_192646 to J.O. P.G. (ITMP) The EOSC Future project is co-funded by the European Union Horizon Programme call INFRAEOSC-03-2020—Grant Agreement Number 101017536. Open Access funding enabled and organized by Projekt DEALPeer reviewe

Large-Scale Recombinant Production of the SARS-CoV-2 Proteome for High-Throughput and Structural Biology Applications

The highly infectious disease COVID-19 caused by the Betacoronavirus SARS-CoV-2 poses a severe threat to humanity and demands the redirection of scientific efforts and criteria to organized research projects. The international COVID19-NMR consortium seeks to provide such new approaches by gathering scientific expertise worldwide. In particular, making available viral proteins and RNAs will pave the way to understanding the SARS-CoV-2 molecular components in detail. The research in COVID19-NMR and the resources provided through the consortium are fully disclosed to accelerate access and exploitation. NMR investigations of the viral molecular components are designated to provide the essential basis for further work, including macromolecular interaction studies and high-throughput drug screening. Here, we present the extensive catalog of a holistic SARS-CoV-2 protein preparation approach based on the consortium’s collective efforts. We provide protocols for the large-scale production of more than 80% of all SARS-CoV-2 proteins or essential parts of them. Several of the proteins were produced in more than one laboratory, demonstrating the high interoperability between NMR groups worldwide. For the majority of proteins, we can produce isotope-labeled samples of HSQC-grade. Together with several NMR chemical shift assignments made publicly available on covid19-nmr.com, we here provide highly valuable resources for the production of SARS-CoV-2 proteins in isotope-labeled form

Rationale, study design, and analysis plan of the Alveolar Recruitment for ARDS Trial (ART): Study protocol for a randomized controlled trial

Background: Acute respiratory distress syndrome (ARDS) is associated with high in-hospital mortality. Alveolar recruitment followed by ventilation at optimal titrated PEEP may reduce ventilator-induced lung injury and improve oxygenation in patients with ARDS, but the effects on mortality and other clinical outcomes remain unknown. This article reports the rationale, study design, and analysis plan of the Alveolar Recruitment for ARDS Trial (ART). Methods/Design: ART is a pragmatic, multicenter, randomized (concealed), controlled trial, which aims to determine if maximum stepwise alveolar recruitment associated with PEEP titration is able to increase 28-day survival in patients with ARDS compared to conventional treatment (ARDSNet strategy). We will enroll adult patients with ARDS of less than 72 h duration. The intervention group will receive an alveolar recruitment maneuver, with stepwise increases of PEEP achieving 45 cmH(2)O and peak pressure of 60 cmH2O, followed by ventilation with optimal PEEP titrated according to the static compliance of the respiratory system. In the control group, mechanical ventilation will follow a conventional protocol (ARDSNet). In both groups, we will use controlled volume mode with low tidal volumes (4 to 6 mL/kg of predicted body weight) and targeting plateau pressure <= 30 cmH2O. The primary outcome is 28-day survival, and the secondary outcomes are: length of ICU stay; length of hospital stay; pneumothorax requiring chest tube during first 7 days; barotrauma during first 7 days; mechanical ventilation-free days from days 1 to 28; ICU, in-hospital, and 6-month survival. ART is an event-guided trial planned to last until 520 events (deaths within 28 days) are observed. These events allow detection of a hazard ratio of 0.75, with 90% power and two-tailed type I error of 5%. All analysis will follow the intention-to-treat principle. Discussion: If the ART strategy with maximum recruitment and PEEP titration improves 28-day survival, this will represent a notable advance to the care of ARDS patients. Conversely, if the ART strategy is similar or inferior to the current evidence-based strategy (ARDSNet), this should also change current practice as many institutions routinely employ recruitment maneuvers and set PEEP levels according to some titration method.Hospital do Coracao (HCor) as part of the Program 'Hospitais de Excelencia a Servico do SUS (PROADI-SUS)'Brazilian Ministry of Healt

Structural Analysis of Proteins from Bacterial Secretion Systems and Their Assemblies by NMR Spectroscopy

International audienc

Structure and Assembly of the Enterohemorrhagic Escherichia coli Type 4 Pilus

International audienceBacterial type 4a pili are dynamic surface filaments that promote bacterial adherence, motility, and macromolecular transport. Their genes are highly conserved among enterobacteria and their expression in enterohemorrhagic Escherichia coli (EHEC) promotes adhesion to intestinal epithelia and pro-inflammatory signaling. To define the molecular basis of EHEC pilus assembly, we determined the structure of the periplasmic domain of its major subunit PpdD (PpdDp), a prototype of an enterobacterial pilin subfamily containing two disulfide bonds. The structure of PpdDp, determined by NMR, was then docked into the density envelope of purified EHEC pili obtained by cryoelectron microscopy (cryo-EM). Cryo-EM reconstruction of EHEC pili at ∼8 Å resolution revealed extremely high pilus flexibility correlating with a large extended region of the pilin stem. Systematic mutagenesis combined with functional and interaction analyses identified charged residues essential for pilus assembly. Structural information on exposed regions and interfaces between EHEC pilins is relevant for vaccine and drug discovery

Interaction of a partially disordered antisigma factor with its partner, the signaling domain of the TonB-dependent transporter HasR.

International audienceBacteria use diverse signaling pathways to control gene expression in response to external stimuli. In Gram-negative bacteria, the binding of a nutrient is sensed by an outer membrane transporter. This signal is then transmitted to an antisigma factor and subsequently to the cytoplasm where an ECF sigma factor induces expression of genes related to the acquisition of this nutrient. The molecular interactions involved in this transmembrane signaling are poorly understood and structural data on this family of antisigma factor are rare. Here, we present the first structural study of the periplasmic domain of an antisigma factor and its interaction with the transporter. The study concerns the signaling in the heme acquisition system (Has) of Serratia marcescens. Our data support unprecedented partially disordered periplasmic domain of an anti-sigma factor HasS in contact with a membrane-mimicking environment. We solved the 3D structure of the signaling domain of HasR transporter and identified the residues at the HasS-HasR interface. Their conservation in several bacteria suggests wider significance of the proposed model for the understanding of bacterial transmembrane signaling