La Florera : Polka

https://digitalcommons.library.umaine.edu/mmb-ps/2716/thumbnail.jp

Predilecta

https://digitalcommons.library.umaine.edu/mmb-ps/3556/thumbnail.jp

Experimental–computational study of carbon nanotube effects on mitochondrial respiration: in silico nano-QSPR machine learning models based on new Raman spectra transform with Markov–Shannon entropy invariants

[Abstract] The study of selective toxicity of carbon nanotubes (CNTs) on mitochondria (CNT-mitotoxicity) is of major interest for future biomedical applications. In the current work, the mitochondrial oxygen consumption (E3) is measured under three experimental conditions by exposure to pristine and oxidized CNTs (hydroxylated and carboxylated). Respiratory functional assays showed that the information on the CNT Raman spectroscopy could be useful to predict structural parameters of mitotoxicity induced by CNTs. The in vitro functional assays show that the mitochondrial oxidative phosphorylation by ATP-synthase (or state V3 of respiration) was not perturbed in isolated rat-liver mitochondria. For the first time a star graph (SG) transform of the CNT Raman spectra is proposed in order to obtain the raw information for a nano-QSPR model. Box–Jenkins and perturbation theory operators are used for the SG Shannon entropies. A modified RRegrs methodology is employed to test four regression methods such as multiple linear regression (LM), partial least squares regression (PLS), neural networks regression (NN), and random forest (RF). RF provides the best models to predict the mitochondrial oxygen consumption in the presence of specific CNTs with R2 of 0.998–0.999 and RMSE of 0.0068–0.0133 (training and test subsets). This work is aimed at demonstrating that the SG transform of Raman spectra is useful to encode CNT information, similarly to the SG transform of the blood proteome spectra in cancer or electroencephalograms in epilepsy and also as a prospective chemoinformatics tool for nanorisk assessmentXunta de Galicia; GRC2014/049Xunta de Galicia; R2014/03

Transcriptome analysis of highly purified mouse spermatogenic cell populations: gene expression signatures switch from meiotic-to postmeiotic-related processes at pachytene stage

Top five canonical pathways and molecular and cellular functions from pairwise comparisons of the four cell populations in chronological order (LZ relative to 2C; PS relative to LZ; RS relative to PS). (XLSX 10 kb

World Press Photo 2012: the discursive construction of the Arab Spring

O presente artigo pretende analisar a forma como o fenómeno da Primavera Árabe foi retratado nas fotografias vencedoras do concurso World Press Photo em 2012. As fotografias jornalísticas, embora se apresentem como índices do real, condicionam frequentemente a percepção dos indivíduos e influenciam as suas práticas sociais. Também as fotografias vencedoras do World Press Photo, um dos concursos mais prestigiados de fotojornalismo, em 2012 não fogem ao construtivismo discursivo que molda a representação e a percepção dos acontecimentos. A partir da análise crítica do discurso, nomeadamente dos instrumentos teóricos da semiótica barthesiana e da semiótica social de Gunther Kress e Theo van Leeuwen, procurámos interpretar as 38 fotografias vencedoras do World Press Photo 2012, a fim de reflectirmos sobre o modo como contribuíram para a compreensão da Revolta Árabe e como despertaram o nosso interesse e a nossa imaginação para o desenrolar do conflito.ABSTRACT:This article aims to analyze how the Arab Spring phenomenon was represented in the award-winning photographs of the World Press Photo contest in 2012. Although news photographs are usually seen as indexes of the real, they often limit the perception of individuals and influence their social practices. Also the winning photographs in 2012 of the World Press Photo, one of the most prestigious photojournalism contests, do not escape the discursive constructivism that shapes the representation and perception of events. Drawing from critical discourse analysis, namely from the theoretical tools of barthesian semiotics and Gunther Kress’s and Theo van Leeuwen’s social semiotics, we sought to interpret the 38 award-winning photographs of the World Press Photo contest in 2012, in order to reflect on the way they have contributed to our understanding of the Arab Revolt and aroused our interest and imagination to the unfolding of the conflict.info:eu-repo/semantics/publishedVersio

CONSERVAÇÃO DE HASTES DE GIRASSOL COM A UTILIZAÇÃO DE DIFERENTES DOSES DE SACAROSE

O objetivo deste trabalho foi avaliar o efeito de diferentes doses de sacarose, sobre a longevidade e resistência pós-colheita de hastes de girassol ornamental. O experimento foi conduzido em delineamento inteiramente casualizado com cinco repetições, sendo analisados dois fatores: período de senescência das hastes florais e influência das diferentes doses do conservante. As hastes de girassol da variedade e Z. elegans ‘Scarleas foram adquirida de um campo de cultivo comercial do Estado de São Paulo, município de, e foram colocadas em vasos de acrílico com solução condicionante nas concentrações: 0,5,15,25 e 35 gramas de sacarose por litro e água. Para a avaliação foram atribuídas notas em ordem decrescente de 0 a 5 conforme os diferentes estádios de senescência das hastes do girassol. Houve interação entre os fatores e assim ocorreu influência das diferentes concentrações no tempo. A sacarose na concentração 15 gramas e 25 gramas mais obtiveram resultados inflorescências do girassol, nas condições testadas aumentando os dias de utilização na comercialização e ornamentação

Open Access

Stable SET knockdown in head and neck squamous cell carcinoma promotes cell invasion and the mesenchymal-like phenotype in vitro, as well as necrosis, cisplatin sensitivity and lymph node metastasis in xenograft tumor model

Alfabetización académica, informacional y digital: trabajo conjunto e integración curricular en el desarrollo de competencias en la comunidad universitaria : El caso de la Universidad Nacional de Lanús

Desde 1997, año de comienzo de las actividades académicas de la UNLa, los espacios de Biblioteca y Pedagogía, ambos dependientes de la Secretaría Académica, han planificado y producido acciones conjuntas que evidencian el entramado de las diferentes alfabetizaciones que tienen lugar en el mundo académico y que requiere de los estudiantes competencias, habilidades, destrezas en la búsqueda y tratamiento de la información, aprovechamiento de las herramientas digitales y estrategias de lectura y escritura de los textos propios del estudio en la universidad. Al mismo tiempo, estas acciones conjuntas se han articulado con distintas propuestas curriculares, con carreras como la Licenciatura en Informática Educativa que con la revisión curricular realizada entre 2013 y 2015 modificó su denominación pasando a llamarse Ciclo de Licenciatura en Tecnologías Digitales para la Educación. También con espacios al interior de las carreras y con la propuesta de Informática para todos los estudiantes de la universidad.Eje 3: Tecnologías digitales y entornos virtuales de la enseñanza universitaria: perspectivas actuales y escenarios futuros. Experiencias de enseñanza universitaria en entornos virtualesSecretaría de Asuntos Académico

Transcriptome analysis of highly purified mouse spermatogenic cell populations: gene expression signatures switch from meiotic-to postmeiotic-related processes at pachytene stage

Background: Spermatogenesis is a complex differentiation process that involves the successive and simultaneous execution of three different gene expression programs: mitotic proliferation of spermatogonia, meiosis, and spermiogenesis. Testicular cell heterogeneity has hindered its molecular analyses. Moreover, the characterization of short, poorly represented cell stages such as initial meiotic prophase ones (leptotene and zygotene) has remained elusive, despite their crucial importance for understanding the fundamentals of meiosis. Results: We have developed a flow cytometry-based approach for obtaining highly pure stage-specific spermatogenic cell populations, including early meiotic prophase. Here we combined this methodology with next generation sequencing, which enabled the analysis of meiotic and postmeiotic gene expression signatures in mouse with unprecedented reliability. Interestingly, we found that a considerable number of genes involved in early as well as late meiotic processes are already on at early meiotic prophase, with a high proportion of them being expressed only for the short time lapse of lepto-zygotene stages. Besides, we observed a massive change in gene expression patterns during medium meiotic prophase (pachytene) when mostly genes related to spermiogenesis and sperm function are already turned on. This indicates that the transcriptional switch from meiosis to post-meiosis takes place very early, during meiotic prophase, thus disclosing a higher incidence of post-transcriptional regulation in spermatogenesis than previously reported. Moreover, we found that a good proportion of the differential gene expression in spermiogenesis corresponds to up-regulation of genes whose expression starts earlier, at pachytene stage this includes transition protein-and protamine-coding genes, which have long been claimed to switch on during spermiogenesis. In addition, our results afford new insights concerning X chromosome meiotic inactivation and reactivation. Conclusions: This work provides for the first time an overview of the time course for the massive onset and turning off of the meiotic and spermiogenic genetic programs. Importantly, our data represent a highly reliable information set about gene expression in pure testicular cell populations including early meiotic prophase, for further data mining towards the elucidation of the molecular bases of male reproduction in mammals