Prorenin anno 2008

For many years, prorenin has been considered to be nothing more than the inactive precursor of renin. Yet, its elevated levels in diabetic subjects with microvascular complications and its extrarenal production at various sites in the body suggest otherwise. This review discusses the origin, regulation, and enzymatic activity of prorenin, its role during renin inhibition, and the angiotensin-dependent and angiotensin-independent consequences of its binding to the recently discovered (pro)renin receptor. The review ends with the concept that prorenin rather than renin determines tissue angiotensin generation

Patients diabétiques de type 2 et réseau social Facebook : retour d’expérience et perspectives d’amélioration de l’accompagnement du patient diabétique

Introduction : Le diabète, représenté dans plus de 90% des cas par le diabète de type 2, est une maladie chronique aux répercussions médicales, psychologiques et sociales. Les réseaux sociaux, notamment Facebook, offrent aux patients diabétiques différents espaces pour s’informer et échanger autour de leur pathologie. L’étude de cet usage permet de s’enquérir du regard intime des patients diabétiques de type 2 notamment par leur recours aux groupes de discussion qui leur sont dédiés. Le travail de cette thèse consistait à explorer les motivations et les retours d’expérience du recours à ces groupes de discussion et d’apporter un éclairage sur les pistes d’améliorations de l’accompagnement des patients diabétiques

Crb3 is required to organize the apical domain of multiciliated cells

International audienceCell shape changes mainly rely on the remodeling of the actin cytoskeleton. Multiciliated cells (MCCs) of the mucociliary epidermis of Xenopus laevis embryos, as they mature, dramatically reshape their apical domain to grow cilia, in coordination with the underlying actin cytoskeleton. Crumbs (Crb) proteins are multifaceted transmembrane apical polarity proteins known to recruit actin linkers and promote apical membrane growth. Here, we identify the homeolog Crb3.L as an important player for centrioles/basal bodies (BB) migration and apical domain morphogenesis in MCCs. Crb3.L is present in cytoplasmic vesicles close to the ascending centrioles/BBs where it partially colocalizes with Rab11a. Crb3.L morpholino-mediated depletion in MCCs caused abnormal migration of centrioles/BBs, reduction of their apical surface, disorganization of their apical actin meshwork, and defective ciliogenesis. Rab11a morpholino-mediated depletion phenocopied Crb3.L loss-of-function in MCCs. Thus, the control of centrioles/BBs migration by Crb3.L might be mediated by Rab11adependent apical trafficking. Furthermore, we show that both phospho-activated ERM (pERM) and Crb3.L are recruited to the growing apical domain of MCCs, where Crb3.L likely anchors pERM allowing actin-dependent expansion of the apical membrane