Desarrollo de la página web específica del máster universitario en psicogerontología de la usal

Memoria ID12-0025. Ayudas de la Universidad de Salamanca para la innovación docente, curso 2012-2013

A Decision Support System for Water Optimization in Anti-Frost Techniques by Sprinklers

[EN] Precision agriculture is a growing sector that improves traditional agricultural processes through the use of new technologies. In southeast Spain, farmers are continuously fighting against harsh conditions caused by the effects of climate change. Among these problems, the great variability of temperatures (up to 20 degrees C in the same day) stands out. This causes the stone fruit trees to flower prematurely and the low winter temperatures freeze the flower causing the loss of the crop. Farmers use anti-freeze techniques to prevent crop loss and the most widely used techniques are those that use water irrigation as they are cheaper than other techniques. However, these techniques waste too much water and it is a scarce resource, especially in this area. In this article, we propose a novel intelligent Internet of Things (IoT) monitoring system to optimize the use of water in these anti-frost techniques while minimizing crop loss. The intelligent component of the IoT system is designed using an approach based on a multivariate Long Short-Term Memory (LSTM) model, designed to predict low temperatures. We compare the proposed approach of multivariate model with the univariate counterpart version to figure out which model obtains better accuracy to predict low temperatures. An accurate prediction of low temperatures would translate into significant water savings, as anti-frost techniques would not be activated without being necessary. Our experimental results show that the proposed multivariate LSTM approach improves the univariate counterpart version, obtaining an average quadratic error no greater than 0.65 degrees C and a coefficient of determination R2 greater than 0.97. The proposed system has been deployed and is currently operating in a real environment obtained satisfactory performance.This work has been partially supported by the Spanish Ministry of Science and Innovation, under the Ramon y Cajal Program (Grant No. RYC2018-025580-I) and under grants RTI2018-096384-B-I00, RTC-2017-6389-5 and RTC2019-007159-5, by the Fundacion Seneca del Centro de Coordinacion de la Investigacion de la Region de Murcia under Project 20813/PI/18, and by the "Conselleria de Educacion, Investigacion, Cultura y Deporte, Direccio General de Ciencia i Investigacio, Proyectos AICO/2020", Spain, under Grant AICO/2020/302.Guillén-Navarro, MA.; Martínez-España, R.; Bueno-Crespo, A.; Morales-García, J.; Ayuso, B.; Cecilia-Canales, JM. (2020). A Decision Support System for Water Optimization in Anti-Frost Techniques by Sprinklers. Sensors. 20(24):1-15. https://doi.org/10.3390/s20247129S1152024Melgarejo-Moreno, J., López-Ortiz, M.-I., & Fernández-Aracil, P. (2019). Water distribution management in South-East Spain: A guaranteed system in a context of scarce resources. Science of The Total Environment, 648, 1384-1393. doi:10.1016/j.scitotenv.2018.08.263Ferrández-Pastor, F., García-Chamizo, J., Nieto-Hidalgo, M., & Mora-Martínez, J. (2018). Precision Agriculture Design Method Using a Distributed Computing Architecture on Internet of Things Context. Sensors, 18(6), 1731. doi:10.3390/s18061731Liaghat. (2010). A Review: The Role of Remote Sensing in Precision Agriculture. American Journal of Agricultural and Biological Sciences, 5(1), 50-55. doi:10.3844/ajabssp.2010.50.55Nelson, G. C., van der Mensbrugghe, D., Ahammad, H., Blanc, E., Calvin, K., Hasegawa, T., … Willenbockel, D. (2013). Agriculture and climate change in global scenarios: why don’t the models agree. Agricultural Economics, 45(1), 85-101. doi:10.1111/agec.12091Crookston, R. K. (2006). A Top 10 List of Developments and Issues Impacting Crop Management and Ecology During the Past 50 Years. Crop Science, 46(5), 2253-2262. doi:10.2135/cropsci2005.11.0416gasDutta, R., Morshed, A., Aryal, J., D’Este, C., & Das, A. (2014). Development of an intelligent environmental knowledge system for sustainable agricultural decision support. Environmental Modelling & Software, 52, 264-272. doi:10.1016/j.envsoft.2013.10.004Zhang, J., Zhu, Y., Zhang, X., Ye, M., & Yang, J. (2018). Developing a Long Short-Term Memory (LSTM) based model for predicting water table depth in agricultural areas. Journal of Hydrology, 561, 918-929. doi:10.1016/j.jhydrol.2018.04.065Sahoo, S., Russo, T. A., Elliott, J., & Foster, I. (2017). Machine learning algorithms for modeling groundwater level changes in agricultural regions of the U.S. Water Resources Research, 53(5), 3878-3895. doi:10.1002/2016wr019933Coopersmith, E. J., Minsker, B. S., Wenzel, C. E., & Gilmore, B. J. (2014). Machine learning assessments of soil drying for agricultural planning. Computers and Electronics in Agriculture, 104, 93-104. doi:10.1016/j.compag.2014.04.004Mohammadi, K., Shamshirband, S., Motamedi, S., Petković, D., Hashim, R., & Gocic, M. (2015). Extreme learning machine based prediction of daily dew point temperature. Computers and Electronics in Agriculture, 117, 214-225. doi:10.1016/j.compag.2015.08.008Feng, Y., Peng, Y., Cui, N., Gong, D., & Zhang, K. (2017). Modeling reference evapotranspiration using extreme learning machine and generalized regression neural network only with temperature data. Computers and Electronics in Agriculture, 136, 71-78. doi:10.1016/j.compag.2017.01.027Jin, X.-B., Yu, X.-H., Wang, X.-Y., Bai, Y.-T., Su, T.-L., & Kong, J.-L. (2020). Deep Learning Predictor for Sustainable Precision Agriculture Based on Internet of Things System. Sustainability, 12(4), 1433. doi:10.3390/su12041433Castañeda-Miranda, A., & Castaño-Meneses, V. M. (2020). Internet of things for smart farming and frost intelligent control in greenhouses. Computers and Electronics in Agriculture, 176, 105614. doi:10.1016/j.compag.2020.105614Tzounis, A., Katsoulas, N., Bartzanas, T., & Kittas, C. (2017). Internet of Things in agriculture, recent advances and future challenges. Biosystems Engineering, 164, 31-48. doi:10.1016/j.biosystemseng.2017.09.007Shi, X., An, X., Zhao, Q., Liu, H., Xia, L., Sun, X., & Guo, Y. (2019). State-of-the-Art Internet of Things in Protected Agriculture. Sensors, 19(8), 1833. doi:10.3390/s19081833Jawad, H., Nordin, R., Gharghan, S., Jawad, A., & Ismail, M. (2017). Energy-Efficient Wireless Sensor Networks for Precision Agriculture: A Review. Sensors, 17(8), 1781. doi:10.3390/s17081781Guillén‐Navarro, M. A., Martínez‐España, R., López, B., & Cecilia, J. M. (2019). A high‐performance IoT solution to reduce frost damages in stone fruits. Concurrency and Computation: Practice and Experience, 33(2). doi:10.1002/cpe.5299Guillén, M. A., Llanes, A., Imbernón, B., Martínez-España, R., Bueno-Crespo, A., Cano, J.-C., & Cecilia, J. M. (2020). Performance evaluation of edge-computing platforms for the prediction of low temperatures in agriculture using deep learning. The Journal of Supercomputing, 77(1), 818-840. doi:10.1007/s11227-020-03288-

Programa Intergeneracional de coaprendizaje: transmisión de valores en la etapa infantil a través de varias generaciones

Memoria ID12-0210. Ayudas de la Universidad de Salamanca para la innovación docente, curso 2012-2013

Antisense Oligonucleotide Rescue of Deep-Intronic Variants Activating Pseudoexons in the 6-Pyruvoyl-Tetrahydropterin Synthase Gene

We report two new 6-pyruvoyl-tetrahydropterin synthase splicing variants identified through genomic sequencing and transcript analysis in a patient with tetrahydrobiopterin deficiency, presenting with hyperphenylalaninemia and monoamine neurotransmitter deficiency. Variant c.243 + 3A>G causes exon 4 skipping. The deep-intronic c.164-672C>T variant creates a potential 5' splice site that leads to the inclusion of four overlapping pseudoexons, corresponding to exonizations of an antisense short interspersed nuclear element AluSq repeat sequence. Two of the identified pseudoexons have been reported previously, activated by different deep-intronic variants, and were also detected at residual levels in control cells. Interestingly, the predominant pseudoexon is nearly identical to a disease causing activated pseudoexon in the F8 gene, with the same 3' and 5' splice sites. Splice switching antisense oligonucleotides (SSOs) were designed to hybridize with splice sites and/or predicted binding sites for regulatory splice factors. Different SSOs corrected the aberrant pseudoexon inclusion, both in minigenes and in fibroblasts from patients carrying the new variant c.164-672C>T or the previously described c.164-716A>T. With SSO treatment PTPS protein was recovered, illustrating the therapeutic potential of the approach, for patients with different pseudoexon activating variants in the region. In addition, the natural presence of pseudoexons in the wild type context suggests the possibility of applying the antisense strategy in patients with hypomorphic PTS variants with the purpose of upregulating their expression to increase overall protein and activity

University teachers' attitudes towards the educational use of ICTS: the case study at the University of Valladolid

Producción CientíficaDebido al proceso de adaptación y convergencia en el que nos hallamos inmersos en la actualidad y a la incorporación de los nuevos planes europeos de enseñanzas universitarias, resulta necesario plantear la inclusión de metodologías activas en el proceso de enseñanza-aprendizaje que, por una parte, prioricen la participación activa de los alumnos en este proceso y, por otra, establezcan una relación diferente entre docentes y discentes. Son muchas las acciones de innovación educativa que se están poniendo en práctica en la Universidad española, con el fin de superar los esquemas didácticos tradicionales y lograr una enseñanza de calidad, objetivos fundamentales del proceso de convergencia europea que se está viviendo en la actualidad. El éxito de estas iniciativas, muchas de ellas apoyadas en las denominadas Tecnologías de la Información y de la Comunicación (TIC) depende, por una parte, de la actitud y del compromiso de los docentes con el cambio metodológico y, por otro, del apoyo que se les brinda desde la institución universitaria para satisfacer sus necesidades formativas y facilitar, así, la incorporación de estos nuevos recursos al proceso de enseñanza-aprendizaje (E/A). Este artículo presenta un estudio de las actitudes de los profesores de la Facultad de Traducción e Interpretación de la Universidad de Valladolid (España) hacia la integración de las TIC en su práctica docente, ya que consideramos que la dimensión actitudinal representa un elemento clave para la renovación pedagógica exigida por el Espacio Europeo de Educación Superior (EEES).The process of European convergence and harmonization and the EHEA-adaptation plan that our universities are implementing nowadays entail not only a methodological change- that is the introduction of active methodologies that emphasize the role played by students in their own learning process- but also a change in the role assumed by teachers in the teaching-learning process. Spanish universities are currently carrying out many experimental and innovative educational projects which try to overcome traditional methodological limitations in order to guarantee solid and quality educational programs in our higher education institutions. Achieving success in innovative instructional practices-most of them based on ICTs- depends both on university teachers' attitude and commitment to the methodological changes involved and on the institutional and organizational support provided by universities in order to enhance ICT competence development of teachers. That is teachers’ attitudes towards ICTs, which in most cases are related to their ICT competence level, play a crucial role in implementing new innovative tools in the teaching-learning process. This research article shows the attitudes of a group of teachers of the Faculty of Translation and Interpreting (University of Valladolid) to the educational use of ICTs, as we consider that the attitudinal dimension is a key issue in the integration of technology into educational environments

Chloroquine-induced DNA damage synergizes with DNA repair inhibitors causing cancer cell death

BackgroundCancer is a global health problem accounting for nearly one in six deaths worldwide. Conventional treatments together with new therapies have increased survival to this devastating disease. However, the persistent challenges of treatment resistance and the limited therapeutic arsenal available for specific cancer types still make research in new therapeutic strategies an urgent need.MethodsChloroquine was tested in combination with different drugs (Panobinostat, KU-57788 and NU-7026) in 8 human-derived cancer cells lines (colorectal: HCT116 and HT29; breast: MDA-MB-231 and HCC1937; glioblastoma: A-172 and LN-18; head and neck: CAL-33 and 32816). Drug´s effect on proliferation was tested by MTT assays and cell death was assessed by Anexin V-PI apoptosis assays. The presence of DNA double-strand breaks was analyzed by phospho-H2AX fluorescent staining. To measure homologous recombination efficiency the HR-GFP reporter was used, which allows flow cytometry-based detection of HR stimulated by I-SceI endonuclease-induced DSBs.ResultsThe combination of chloroquine with any of the drugs employed displayed potent synergistic effects on apoptosis induction, with particularly pronounced efficacy observed in glioblastoma and head and neck cancer cell lines. We found that chloroquine produced DNA double strand breaks that depended on reactive oxygen species formation, whereas Panobinostat inhibited DNA double-strand breaks repair by homologous recombination. Cell death caused by chloroquine/Panobinostat combination were significantly reduced by N-Acetylcysteine, a reactive oxygen species scavenger, underscoring the pivotal role of DSB generation in CQ/LBH-induced lethality. Based on these data, we also explored the combination of CQ with KU-57788 and NU-7026, two inhibitors of the other main DSB repair pathway, nonhomologous end joining (NHEJ), and again synergistic effects on apoptosis induction were observed.ConclusionOur data provide a rationale for the clinical investigation of CQ in combination with DSB inhibitors for the treatment of different solid tumors

Protocolo del cuestionario para identificar el ejercicio de participación de jóvenes de 12 a 16 años que estudian en INS de la ciudad de L’Hospitalet de Llobregat.

Los objetivos específicos que este instrumento ha permitido cubrir la información respecto a las siguientes dimensiones: 1. El concepto de participación 2. El nivel (grados de participación) 3. Los espacios de participación (físicos y “online”) 4. Las consecuencias de la participación 5. Propuestas, acciones, actividades que realizan los jóvene

Pathogenic variants of DNAJC12 and evaluation of the encoded cochaperone as a genetic modifier of hyperphenylalaninemia

This is the peer reviewed version of the following article: Pathogenic variants of DNAJC12 and evaluation of the encoded cochaperone as a genetic modifier of hyperphenylalaninemia. Human Mutation (2020): 25 April, which has been published in final form at [https://doi.org/10.1002/humu.24026. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived VersionsThe variants identified in this study are openly available at http://www.lovd.nl/ with reference numbers 0000644164, 0000645396, 0000644166, and 0000405673Biallelic variants of the gene DNAJC12, which encodes a cochaperone, were recently described in patients with hyperphenylalaninemia (HPA). This paper reports the retrospective genetic analysis of a cohort of unsolved cases of HPA. Biallelic variants of DNAJC12 were identified in 20 patients (generally neurologically asymptomatic) previously diagnosed with phenylalanine hydroxylase (PAH) deficiency (phenylketonuria [PKU]). Further, mutations of DNAJC12 were identified in four carriers of a pathogenic variant of PAH. The genetic spectrum of DNAJC12 in the present patients included four new variants, two intronic changes c.298‐2A>C and c.502+1G>C, presumably affecting the splicing process, and two exonic changes c.309G>T (p.Trp103Cys) and c.524G>A (p.Trp175Ter), classified as variants of unknown clinical significance (VUS). The variant p.Trp175Ter was detected in 83% of the mutant alleles, with 14 cases homozygous, and was present in 0.3% of a Spanish control population. Functional analysis indicated a significant reduction in PAH and its activity, reduced tyrosine hydroxylase stability, but no effect on tryptophan hydroxylase 2 stability, classifying the two VUS as pathogenic variants. Additionally, the effect of the overexpression of DNAJC12 on some destabilizing PAH mutations was examined and a mutation‐specific effect on stabilization was detected suggesting that the proteostasis network could be a genetic modifier of PAH deficiency and a potential target for developing mutation‐specific treatments for PKUThis work was funded by grant PI16/00573, B2017/BMD-3721, the Fundación Isabel Gemio and the Fundación La Caixa (LCF/PR/PR16/11110018), an institutional grant from the Fundación Ramón Areces to the Centro de Biología Molecular Severo Ochoa, and the European Regional Development Fun

Integrated flow cytometry and sequencing to reconstruct evolutionary patterns from dysplasia to acute myeloid leukemia

Clonal evolution in acute myeloid leukemia (AML) originates long before diagnosis and is a dynamic process that may affect survival. However, it remains uninvestigated during routine diagnostic workups. We hypothesized that the mutational status of bone marrow dysplastic cells and leukemic blasts, analyzed at the onset of AML using integrated multidimensional flow cytometry (MFC) immunophenotyping and fluorescence-activated cell sorting (FACS) with next-generation sequencing (NGS), could reconstruct leukemogenesis. Dysplastic cells were detected by MFC in 285 of 348 (82%) newly diagnosed patients with AML. Presence of dysplasia according to MFC and World Health Organization criteria had no prognostic value in older adults. NGS of dysplastic cells and blasts isolated at diagnosis identified 3 evolutionary patterns: stable (n = 12 of 21), branching (n = 4 of 21), and clonal evolution (n = 5 of 21). In patients achieving complete response (CR), integrated MFC and FACS with NGS showed persistent measurable residual disease (MRD) in phenotypically normal cell types, as well as the acquisition of genetic traits associated with treatment resistance. Furthermore, whole-exome sequencing of dysplastic and leukemic cells at diagnosis and of MRD uncovered different clonal involvement in dysplastic myelo-erythropoiesis, leukemic transformation, and chemoresistance. Altogether, we showed that it is possible to reconstruct leukemogenesis in ∼80% of patients with newly diagnosed AML, using techniques other than single-cell multiomics.This work was supported by grants from the Área de Oncología del Instituto de Salud Carlos III, Centro de Investigación Biomédica en Red (CIBER-ONC) (CB16/12/00369, CB16/12/00233, CB16/12/00489, and CB16/12/00284), Instituto de Salud Carlos III/Subdirección General de Investigación Sanitaria (FIS numbers PI16/01661, PI16/00517, and PI19/01518), and the Plan de Investigación de la Universidad de Navarra (PIUNA 2014-18). This work was supported internationally by the Cancer Research UK, FCAECC, and AIRC under the Accelerator Award Program (EDITOR)

La grabación sonora: un recurso pedagógico multidisciplinar para la reinterpretación de la Historia

Mediante el presente proyecto se ha pretendido abordar un nuevo acercamiento a la historia de la música, en tanto manifestación cultural, basado en una metodología de reciente creación que entronca directamente con los estudios sobre la praxis interpretativa. Para ello hemos profundizado en el correcto manejo de los software informáticos que permiten el análisis de estas fuentes sonoras (fundamentalmente visualizadores de ondas y editores de sonido). Como resultado, hemos constituido un grupo de trabajo abierto a profesores y alumnos, ubicado físicamente en la Facultad de Geografía e Historia de la UCM