10 research outputs found
Shallow Silicon Vacancy Centers with lifetime-limited optical linewidths in Diamond Nanostructures
The negatively charged silicon vacancy center (SiV) in diamond is a
promising, yet underexplored candidate for single-spin quantum sensing at
sub-kelvin temperatures and tesla-range magnetic fields. A key ingredient for
such applications is the ability to perform all-optical, coherent addressing of
the electronic spin of near-surface SiV centers. We present a robust and
scalable approach for creating individual, 50nm deep SiV with
lifetime-limited optical linewidths in diamond nanopillars through an
easy-to-realize and persistent optical charge-stabilization scheme. The latter
is based on single, prolonged 445nm laser illumination that enables continuous
photoluminescence excitation spectroscopy, without the need for any further
charge stabilization or repumping. Our results constitute a key step towards
the use of near-surface, optically coherent SiV for sensing under extreme
conditions, and offer a powerful approach for stabilizing the
charge-environment of diamond color centers for quantum technology
applications.Comment: 15 pages, 13 figures including supplementary informatio
Low temperature photo-physics of single NV centers in diamond
International audienceWe investigate the magnetic field dependent photo-physics of individual Nitrogen-Vacancy (NV) color centers in diamond under cryogenic conditions. At distinct magnetic fields, we observe significant reductions in the NV photoluminescence rate, which indicate a marked decrease in the optical readout efficiency of the NV's ground state spin. We assign these dips to excited state level anti-crossings, which occur at magnetic fields that strongly depend on the effective, local strain environment of the NV center. Our results offer new insights into the structure of the NVs' excited states and a new tool for their effective characterization. Using this tool, we observe strong indications for strain-dependent variations of the NV's orbital g-factor, obtain new insights into NV charge state dynamics, and draw important conclusions regarding the applicability of NV centers for low-temperature quantum sensing
Transcriptional induction of slit diaphragm genes by Lmx1b is required in podocyte differentiation
LMX1B encodes a LIM-homeodomain transcription factor. Mutations in LMX1B cause nail-patella syndrome (NPS), an autosomal dominant disease with skeletal abnormalities, nail hypoplasia, and nephropathy. Expression of glomerular basement membrane (GBM) collagens is reduced in Lmx1b(–/–) mice, suggesting one basis for NPS nephropathy. Here, we show that Lmx1b(–/–) podocytes have reduced numbers of foot processes, are dysplastic, and lack typical slit diaphragms, indicating an arrest in development. Using antibodies to podocyte proteins important for podocyte function, we found that Lmx1b(–/–) podocytes express near-normal levels of nephrin, synaptopodin, ZO-1, α3 integrin, and GBM laminins. However, mRNA and protein levels for CD2AP and podocin were greatly reduced, suggesting a cooperative role for these molecules in foot process and slit diaphragm formation. We identified several LMX1B binding sites in the putative regulatory regions of both CD2AP and NPHS2 (podocin) and demonstrated that LMX1B binds to these sequences in vitro and can activate transcription through them in cotransfection assays. Thus, LMX1B regulates the expression of multiple podocyte genes critical for podocyte differentiation and function. Our results indicate that reduced levels of proteins associated with foot processes and the glomerular slit diaphragm likely contribute, along with reduced levels of GBM collagens, to the nephropathy associated with NPS
Transcriptional induction of slit diaphragm genes by Lmx1b is required in podocyte differentiation
Doublecortin May Play a Role in Defining Chondrocyte Phenotype
Embryonic development of articular cartilage has not been well understood and the role of doublecortin (DCX) in determination of chondrocyte phenotype is unknown. Here, we use a DCX promoter-driven eGFP reporter mouse model to study the dynamic gene expression profiles in mouse embryonic handplates at E12.5 to E13.5 when the condensed mesenchymal cells differentiate into either endochondral chondrocytes or joint interzone cells. Illumina microarray analysis identified a variety of genes that were expressed differentially in the different regions of mouse handplate. The unique expression patterns of many genes were revealed. Cytl1 and 3110032G18RIK were highly expressed in the proximal region of E12.5 handplate and the carpal region of E13.5 handplate, whereas Olfr538, Kctd15, and Cited1 were highly expressed in the distal region of E12.5 and the metacarpal region of E13.5 handplates. There was an increasing gradient of Hrc expression in the proximal to distal direction in E13.5 handplate. Furthermore, when human DCX protein was expressed in human adipose stem cells, collagen II was decreased while aggrecan, matrilin 2, and GDF5 were increased during the 14-day pellet culture. These findings suggest that DCX may play a role in defining chondrocyte phenotype