Projeto e Desenvolvimento de Fechadura Eletrônica controlada pela Internet

O controle de acesso a ambientes pode ser realizado de diversas formas: das tradicionais fechaduras com chaves mecânicas aos sistemas que utilizam reconhecimento biométrico. Há, ainda, as fechaduras eletrônicas que permitem a abertura de portas por meio de um browser. Considerando a importância de fornecer controle de acesso a ambientes e a dsponibilidade de dispositivos eletrônicos que podem ser programados para realizar este controle, foi realizado um projeto com o objetivo de desenvolver um sistema para controle de uma fechadura eletrônica com abertura por meio de um browser Internet, via RFID e teclado numérico. Por meio de um navegador é também realizada a manutenção dos usuários e a visualização de logs de acesso, armazenado em um cartão micro SD. O projeto foi desenvolvido com um microcontrolador ATmega 2560 integrado no kit de desenvolvimento Arduino

Contagem por NMP e sorovares de Salmonella isoladas de carcaças de frangos resfriadas

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Contagem por NMP e sorovares de Salmonella isoladas de carcaças de frangos resfriadas

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Evaluation of the cytotoxic and genotoxic effects of Sida planicaulis Cav extract using human neuroblastoma cell line SH-SY5Y

Sida planicaulis is a weed thought to have originated in Brazil, where it is present in abundant quantities, but also this plant is also found in south-central Florida, Indian Ocean Islands, and the Pacific Islands. Sida planicaulis produces neurotoxicity that adversely affects livestock breeding with heavy animal losses and consequent negative impact on Brazil’s economy. The aim of this study was to determine the chemical profile, cytotoxic and genotoxic effects of ethanolic extracts of S. planicaulis collected in winter (leaf extract) and summer (leaf extract and leaf + flower extract) using an in vitro model of human neuroblastoma cell line SH-SY5Y. Phytochemical screening demonstrated the presence of alkaloids, flavonoids, and apolar compounds. Rutin, quercetin, and swainsonine were detected by HPLC and GC/MS, respectively. Phosphorus, potassium, iron, and zinc were the inorganic elements found. Extracts produced cytotoxicity at all concentrations tested (7–4,000 μg/ml) as evidenced by the colorimetric assay [3-(4,5-dimethyl-thiazol-2-yl) −2,5-diphenyltetrazolium bromide (MTT)]. Based upon the alkaline comet assay extracts were found to induce genotoxicity at concentrations ranging from 0.437 to 7 μg/ml. DNA damage produced by extracts was affirmed using a modified comet assay with the enzymes Endo III and FPG in a concentration dependent manner. Further, enzyme-modified comet assay showed both oxidized purines and pyrimidines, and consequently oxidative stress was related to genomic instability and cell death. Data suggest that low concentrations of ethanolic extracts of S. planicaulis (different seasons) induced increased DNA damage related to oxidative stress and chemical composition

Rhipicephalus sanguineus (ACARI: IXODIDAE) BITING A HUMAN BEING IN PORTO ALEGRE CITY, RIO GRANDE DO SUL, BRAZIL

We report the finding of a female brown dog tick, Rhipicephalus sanguineus (Acari: Ixodidae) on the scalp of a male patient inPorto Alegre, Rio Grande do Sul, Brazil. Human parasitism by this tick is rare and has seldomly been reported in the literature, despite its recognized importance since it can act as a vector of Rickettsia rickettsii, the agent of spotted fever