Design of mechatronic systems through aspect and object-oriented modeling

Design of mechatronic systems involves the use of multiple disciplines, from mechanics to electronics and computer science. Different granularities of hybrid co-simulations with increasing details can be used during the design process. However, there is the need of modeling tools for effectively managing the necessary abstraction layers. This work proposes a combination of Aspect-Oriented and Object-Oriented modeling for reaching the goal. Moreover, it shows how the utilization of these tools can facilitate design-space exploration, segregation of domains of expertise and enhances co-design

Dermoscopy and Reflectance Confocal Microscopy for Monitoring the Treatment of Actinic Keratosis with Ingenol Mebutate Gel: Report of Two Cases

Introduction: A relatively novel application for dermoscopy and reflectance confocal microscopy (RCM) is their use in the monitoring of topical treatment response for non-melanoma skin cancer. Actinic keratosis (AK) is the early phase of a multistep biologic continuum leading to invasive squamous cell carcinoma. A number of topical therapies are now available for the treatment of AK but their disadvantages include long treatment duration and prolonged local reactions. Ingenol mebutate is a newer therapy for AK which is only applied for 2 or 3 days. Case Report: Dermoscopy and RCM findings in two patients with AK treated with ingenol mebutate confirm that it induces rapid lesion necrosis and specific neutrophil-mediated, antibody-dependent cellular cytotoxicity. Necrosis occurs via mitochondrial membrane disruption, with subsequent eradication of residual tumor cells via transient inflammation. Local skin reactions to ingenol mebutate should be considered part of the drug’s mechanism of action rather than an adverse effect. Conclusion: Ingenol mebutate is a valuable therapy for the treatment of AK. This case report adds further evidence to the usefulness of dermoscopy and RCM in the assessment and monitoring of treatment outcome

Periosteal derived cells and bone tissue regenerative medicine

Mesenchymal stem cells (MSCs), showing a high capacity of self-renewal and differentiation into various lineages, have primarily used for the biological repair of cartilage and bone. Even though MSCs have been identified in different organ tissues, cells from different sources may show phenotypic heterogeneity, different in vivo results and specific functions of graft regions after transplantation. Therefore, a correct selection of MSC source is crucial to obtain a more efficient treatment for regeneration of injured osteochondral tissues. Periosteum Derived Progenitor Cells (PDPCs), which possesses multipotency at single cell level and can form cartilage and bone in vitro and in vivo may represent as an interesting cell resource for bone tissue engineering. Aim of the present study is the isolation and characterization of human PDPCs and the evaluation of their ability to grow on bioresorbable composite scaffolds for bone tissue engineering applications. PDCPs were obtained from periosteal tissue harvested from healthy subjects undergoing surgery for orthopedic trauma. Prior to cells seeding cell were phenotipically characterized. Three composite scaffolds, differing in weight ratios between the components were tested. The scaffolds were coded as CEL2/POL 0/100, 40/60 and 70/30 where CEL2 is a bioglass and POL the organic component based on chitosan and gelatin. Cells were cultured for 14 and 21 days. Our culture conditions favour the selection of a mesenchymal stem cells population. The obtained PDPCs displayed a good ability to interact with the different tested scaffolds. Morphological and biochemical analysis performed showed that cells maintain their metabolic activity and ability to proliferate on the scaffolds. Differentiation over proliferation that occurred to PDCPs at the increase of bioactive glass concentration proves the capacity of these scaffolds to modulate osteogenic properties. This strengthens the hypothesis of periosteum as stem cell source for an osteochondral tissue regenerative medicine based on in situ cell recruitment. This work was supported by RBAP10MLK7 FIRB project

Contributo al femminile all’educazione tecnica e scientifica per le materie STEM. Fare rete tra scienziate/i, enti, università, associazioni, media e portatori d’interesse

Questo “contributo al femminile” è stato realizzato da un gruppo di perso- ne che ambisce a contribuire al miglioramento dell’educazione scolastica; è destinato a donne, uomini, ragazze, ragazzi, bambine e bambini per mettere in luce potenzialità, diseguaglianze, specificità di vita e professionali e favorire la parità di genere soprattutto negli ambiti tecnico-scientifici, in particolar modo nelle discipline STEM (Science, Technology, Engineering, Mathematics)

Influenza vaccination coverage among medical residents: An Italian multicenter survey

Although influenza vaccination is recognized to be safe and effective, recent studies have confirmed that immunization coverage among health care workers remain generally low, especially among medical residents (MRs). Aim of the present multicenter study was to investigate attitudes and determinants associated with acceptance of influenza vaccination among Italian MRs. A survey was performed in 2012 on MRs attending post-graduate schools of 18 Italian Universities. Each participant was interviewed via an anonymous, self-administered, web-based questionnaire including questions on attitudes regarding influenza vaccination. A total of 2506 MRs were recruited in the survey and 299 (11.9%) of these stated they had accepted influenza vaccination in 2011-2012 season. Vaccinated MRs were older (P = 0.006), working in clinical settings (P = 0.048), and vaccinated in the 2 previous seasons (P < 0.001 in both seasons). Moreover, MRs who had recommended influenza vaccination to their patients were significantly more compliant with influenza vaccination uptake in 2011-2012 season (P < 0.001). "To avoid spreading influenza among patients" was recognized as the main reason for accepting vaccination by less than 15% of vaccinated MRs. Italian MRs seem to have a very low compliance with influenza vaccination and they seem to accept influenza vaccination as a habit that is unrelated to professional and ethical responsibility. Otherwise, residents who refuse vaccination in the previous seasons usually maintain their behaviors. Promoting correct attitudes and good practice in order to improve the influenza immunization rates of MRs could represent a decisive goal for increasing immunization coverage among health care workers of the future. © 2014 Landes Bioscience

Functional annotation of human long noncoding RNAs via molecular phenotyping

Long noncoding RNAs (lncRNAs) constitute the majority of transcripts in the mammalian genomes, and yet, their functions remain largely unknown. As part of the FANTOM6 project, we systematically knocked down the expression of 285 lncRNAs in human dermal fibroblasts and quantified cellular growth, morphological changes, and transcriptomic responses using Capped Analysis of Gene Expression (CAGE). Antisense oligonucleotides targeting the same lncRNAs exhibited global concordance, and the molecular phenotype, measured by CAGE, recapitulated the observed cellular phenotypes while providing additional insights on the affected genes and pathways. Here, we disseminate the largest-todate lncRNA knockdown data set with molecular phenotyping (over 1000 CAGE deep-sequencing libraries) for further exploration and highlight functional roles for ZNF213-AS1 and lnc-KHDC3L-2.Peer reviewe

A model-based design methodology for the development of mechatronic systems

The development of mechatronic systems involves the use of multiple disciplines, from mechanical engineering to electronics engineering and computer science. Traditionally, every discipline was developed independently and then integrated to generate the final system. However, high-quality designs cannot be achieved without simultaneously considering all the engineering disciplines. This integrated approach carries an intrinsic complexity into system design process and numerous researches are on-going in order to find out optimal methods. This article illustrates a methodology based on Model-Based System Engineering to support the integrated development of complex mechatronic devices. The main contribution is the introduction of a design methodology based on the W model and the identification of SysML as the tool to support the whole process. This method will also address the problem of “devices interchangeability”, that means the possibility to develop the functionality of a system with different operation principles, at a very early stage of the development process (i.e. during the conceptual development). To achieve this goal, the methodology treats the problem of linking the conceptual with executable models to enable the validation by simulation. Main advantages of this methodology are in providing, to the mechatronic systems designers, a fixed schedule which does not limit their intuition and reduces complexity through a hierarchical approach. The process has been tested through the rationalization of the choices that have brought to the current solution of the filling system of an automatic filling machine for liquid foodstuff

Tools for the development of a design methodology for mechatronic systems

Tools for the Development of a Design Methodology for Mechatronic Systems

Overcoming real time bond in high level simulation environments

Overcoming Real Time Bond in High Level Simulation Environment