The methodology of functional modeling as a tool for formalizing the process of intercepting computer information through spurious electromagnetic radiation and interference

The article is devoted to the formalization of the process of unauthorized getting of computer information by intercepting informative signals of secondary electromagnetic radiation of the main technical means and systems by technical means of intelligence, as well as the removal informative guidance signals on the conductive lines of the main technical means by technical means of intelligence. Analyzing the completeness problem of the assessing information leakage threats due to the interception of informative signals of side electromagnetic radiation and leads, it must be stated that the procedure for identifying such threats is limited only to their detection by controlling only individual signs of manifestations of these threats. At the same time, the dynamics of the interception implementation process is not taken into account. This determines the need to solve the scientific problem of developing methodological foundations for the synthesis of algorithms for recognizing information leakage threats. The article presents the main stages of the procedure for formalizing the actions of the violator to intercept information. The basis for solving such problems is the procedure for forming the recognition feature space. In order to form such a space, functional modeling methods are used. The article presents a representation of the functional diagram in terms of the Markov process, as well as obtaining a mathematical expression to determine the average value of the time spent by the violator to achieve the target interception function

Kanamycin alters cytoplasmic and nuclear phosphoinositide signaling in the organ of Corti in vivo

Aminoglycoside antibiotics strongly bind to phosphoinositides and affect their membrane distribution and metabolism. Kanamycin treatment also disrupts Rac/Rho signaling pathways to the actin cytoskeleton in the mouse inner ear in vivo . Here, we investigate the influence of kanamycin on phosphoinositide signaling in sensory cells (hair cells) of the mouse cochlea. Immunoreactivity to phosphatidylinositol-3,4,5-trisphosphate (PIP 3 ) decreased in the organ of Corti, especially in outer hair cells, after 3–7 days of drug treatment, whereas imunoreactivity to phosphatidylinositol-4,5-bisphosphate (PIP 2 ) increased. Immunoreactivity to PIP 2 was present at the apical poles of outer hair cells, but appeared in their nuclei only after drug treatment. Furthermore, nuclear PIP 2 formed a complex with histone H3 and attenuated its acetylation in outer hair cells. In agreement with reduced PIP 3 signaling, phosphorylated Akt decreased in both the cytoplasm and nuclei of outer hair cells after kanamycin treatment. This study suggests that kanamycin disturbs the balance between PIP 2 and PIP 3 , modifies gene transcription via histone acetylation and diminishes the PI3K/Akt survival pathway. These actions may contribute to the death of outer hair cells, which is a consequence of chronic kanamycin treatment.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/65167/1/j.1471-4159.2006.04117.x.pd

Genomic tagging reveals a random association of endogenous PtdIns5P 4-kinases IIα and IIβ and a partial nuclear localization of the IIα isoform

PtdIns5P 4-kinases IIα and IIβ are cytosolic and nuclear respectively when transfected into cells, including DT40 cells [Richardson, Wang, Clarke, Patel and Irvine (2007) Cell. Signalling 19, 1309–1314]. In the present study we have genomically tagged both type II PtdIns5P 4-kinase isoforms in DT40 cells. Immunoprecipitation of either isoform from tagged cells, followed by MS, revealed that they are associated directly with each other, probably by heterodimerization. We quantified the cellular levels of the type II PtdIns5P 4-kinase mRNAs by real-time quantitative PCR and the absolute amount of each isoform in immunoprecipitates by MS using selective reaction monitoring with 14N,13C-labelled internal standard peptides. The results suggest that the dimerization is complete and random, governed solely by the relative concentrations of the two isoforms. Whereas PtdIns5P 4-kinase IIβ is >95% nuclear, as expected, the distribution of PtdIns4P 4-kinase IIα is 60% cytoplasmic (all bound to membranes) and 40% nuclear. In vitro, PtdIns5P 4-kinase IIα was 2000-fold more active as a PtdIns5P 4-kinase than the IIβ isoform. Overall the results suggest a function of PtdIns5P 4-kinase IIβ may be to target the more active IIα isoform into the nucleus

Two novel phosphatidylinositol-4-phosphate 5-kinase type Iγ splice variants expressed in human cells display distinctive cellular targeting

The generation of various phosphoinositide messenger molecules at distinct locations within the cell is mediated via the specific targeting of different isoforms and splice variants of phosphoinositide kinases. The lipid messenger PtdIns(4,5)P2 is generated by several of these enzymes when targeted to distinct cellular compartments. Several splice variants of the type Iγ isoform of PIPK (PtdIns4P 5-kinase), which generate PtdIns(4,5)P2, have been identified, and each splice variant is thought to serve a unique functional role within cells. Here, we have identified two novel C-terminal splice variants of PIPKIγ in human cells consisting of 700 and 707 amino acids. These two splice variants are expressed in multiple tissue types and display PIPK activity in vitro. Interestingly, both of these novel splice variants display distinct subcellular targeting. With the addition of these two new splice isoforms, there are minimally five PIPKIγ splice variants that have been identified in mammals. Therefore, we propose the use of the HUGO (Human Genome Organization) nomenclature in the naming of the splice isoforms. PIPKIγ_i4 (700 amino acids) is present in the nucleus, a targeting pattern that has not been previously observed in any PIPKIγ splice variant. PIPKIγ_i5 (707 amino acids) is targeted to intracellular vesicle-like structures, where it co-localizes with markers of several types of endosomal compartments. As occurs with other PIPKIγ splice variants, the distinctive C-terminal sequences of PIPKIγ_i4 and PIPKIγ_i5 may facilitate association with unique protein targeting factors, thereby localizing the kinases to their appropriate cellular subdomains for the site-specific generation of PtdIns(4,5)P2

Nuclear Inositide Signaling Via Phospholipase C

The existence of an independent nuclear inositide pathway distinct from the cytoplasmic one has been demonstrated in different physiological systems and in diseases. In this prospect we analyze the role of PI-PLC1 nuclear isoform in relation to the cell cycle regulation, the cell differentiation, and different physiopathological pathways focusing on the importance of the nuclear localization from both molecular and clinical point of view. PI-PLC1 is essential for G1/S transition through DAG and Cyclin D3 and plays also a central role in G2/M progression through Cyclin B1 and PKC. In the differentiation process of C2C12 cells PI-PLC1 increases in both myogenic differentiation and osteogenic differentiation. PI-PLC1 and Cyclin D3 reduction has been observed in Myotonic Dystrophy (DM) suggesting a pivotal role of these enzymes in DM physiopathology. PI-PLC1 is also involved in adipogenesis through a double phase mechanism. Moreover, PI-PLC1 plays a key role in the normal hematopoietic differentiation where it seems to decrease in erythroid differentiation and increase in myeloid differentiation. In Myelodysplastic Syndromes (MDS) PI-PLC1 has a genetic and epigenetic relevance and it is related to MDS patients' risk of Acute Myeloid Leukemia (AML) evolution. In MDS patients PI-PLC1 seems to be also a therapeutic predictive outcome marker. In the central nervous system, PI-PLC1 seems to be involved in different pathways in both brain cortex development and synaptic plasticity related to different diseases. Another PI-PLC isozyme that could be related to nuclear activities is PI-PLC that is involved in infertility processes. J. Cell. Biochem. 118: 1969-1978, 2017

Phase interaction in the metal-oxide melts-gas system: the modeling of structure, properties and processes

This monograph describes mathematical models that enable prediction of phase compositions for various technological processes, as developed on the base of a complex physico-chemical analysis of reaction. It studies thermodynamics and kinetics of specific stages of complex pyrometallurgical processes involving boron, carbon, sulfur, tungsten, phosphorus, and many more, as well as their exposure to all sorts of factors. First and foremost, this enables to optimize processes and technologies at the stage of design, while traditional empirical means of development of new technologies are basically incapable of providing an optimal solution. Simulation results of metals and alloys production, welding and coating technologies allow obtaining materials with pre-given composition, structure and properties in a cost-saving and conscious manner. Moreover, a so-called "inverse problem", i.e., selecting source materials which would ensure the required results, cannot be solved by any other means

ARTERIAL HYPERTENSISON IN POSTMENOPAUSAL WOMEN: CURRENT POSSIBILITIES OF MEDICAL THERAPY IN OUT-PATIENTS

Arterial hypertensison in postmenopausal women: current possibilities of medical therapy in out-patients