The Lyngen Gabbro: the lower crust of an Ordovician Incipient-Arc

We present evidence for the origin of the Lyngen Gabbro of the Ordovician Lyngen Magmatic Complex in Troms, Northern Norway. The two magmatic suites of the Lyngen Gabbro strike parallel NNE-SSW, and have distinct magmatic signatures. We define these signatures by using major and trace-element analyses together with selected major- and trace-element mineral analyses and 143Nd/144Nd-isotope whole-rock analyses of gabbroic to tonalitic plutonic rocks from seven detailed cross-sections from this large gabbro-complex. The Western suite of the Lyngen Gabbro precipitated from magma that may have been derived from the same system as the associated volcanic rocks. The gabbros have high An-content (An>90) of their plagioclases relative to co-existing mafic minerals. Together with somewhat high ɛNd(t) values (+6), this implies that the parental magmas were hydrous tholeiites similar to those found in back arc basins today. The Eastern suite, on the other hand, consist of cumulates that were precipitated from melts resembling those of ultra-depleted high-Ca boninitic magmas found in fore-arcs. Extremely high-An plagioclases (An>95) co-exist with evolved mafic minerals and oxides, and the ɛNd(t) values are lower (+4) than in the Western suite. The Eastern suite has no volcanic counterpart, but dikes intersecting the suites have compositions that possibly represent its parental magma. The oceanic Rypdalen Shear Zone generally separates the two suites in the north, but several non-tectonic transitions from boninitic to tholeiitic signatures southwards advocate that the magmatism happened concurrently. The magmatic proximity between the suites, the hydrous magmatism and the absence of a silicic or calc-alkaline mature arc section, suggests that the Lyngen Gabbro formed in the Iapetus Ocean under conditions presently found in incipient arcs later emplaced as outer arc highs

The Methylococcus capsulatus (Bath) Secreted Protein, MopE*, Binds Both Reduced and Oxidized Copper

Under copper limiting growth conditions the methanotrophic bacterium Methylococcus capsulatus (Bath) secrets essentially only one protein, MopE*, to the medium. MopE* is a copper-binding protein whose structure has been determined by X-ray crystallography. The structure of MopE* revealed a unique high affinity copper binding site consisting of two histidine imidazoles and one kynurenine, the latter an oxidation product of Trp130. In this study, we demonstrate that the copper ion coordinated by this strong binding site is in the Cu(I) state when MopE* is isolated from the growth medium of M. capsulatus. The conclusion is based on X-ray Near Edge Absorption spectroscopy (XANES), and Electron Paramagnetic Resonance (EPR) studies. EPR analyses demonstrated that MopE*, in addition to the strong copper-binding site, also binds Cu(II) at two weaker binding sites. Both Cu(II) binding sites have properties typical of non-blue type II Cu (II) centres, and the strongest of the two Cu(II) sites is characterised by a relative high hyperfine coupling of copper (

Tailoring Hydrothermal Vent Biodiversity Toward Improved Biodiscovery Using a Novel in situ Enrichment Strategy

Deep-sea hydrothermal vents are amongst the most extreme environments on Earth and represent interesting targets for marine bioprospecting and biodiscovery. The microbial communities in hydrothermal vents are often dominated by chemolithoautotrophs utilizing simple chemical compounds, though the full extent of their heterotrophic abilities is still being explored. In the bioprocessing industry, where degradation of complex organic materials often is a major challenge, new microbial solutions are heavily needed. To meet these needs, we have developed novel in situ incubators and tested if deployment of recalcitrant materials from fish farming and wood-pulping industries introduced changes in the microbial community structure in hot marine hydrothermal sediments. The incubation chambers were deployed in sediments at the Bruse vent site located within the Jan Mayen vent field for 1 year, after which the microbial populations in the chambers were profiled by 16S rRNA Ion Torrent amplicon sequencing. A total of 921 operational taxonomic units (OTUs) were assigned into 74 different phyla where differences in community structure were observed depending on the incubated material, chamber depth below the sea floor and/or temperature. A high fraction of putative heterotrophic microbial lineages related to cultivated members within the Thermotogales were observed. However, considerable fractions of previously uncultivated and novel Thermotogales and Bacteroidetes were also identified. Moreover, several novel lineages (e.g., members within the DPANN superphylum, unidentified archaeal lineages, unclassified Thermoplasmatales and Candidatus division BRC-1 bacterium) of as-yet uncultivated thermophilic archaea and bacteria were identified. Overall, our data illustrate that amendment of hydrothermal vent communities by in situ incubation of biomass induces shifts in community structure toward increased fractions of heterotrophic microorganisms. The technologies utilized here could aid in subsequent metagenomics-based enzyme discovery for diverse industries.publishedVersio

Vp/Vs-ratios and anisotropy on the northern Jan Mayen Ridge, North Atlantic, determined from ocean bottom seismic data

In order to gain insight into the lithology and crustal evolution of the northern Jan Mayen Ridge, North Atlantic, the horizontal components of an Ocean Bottom Seismometer (OBS) dataset were analyzed with regard to Vp/Vs-modeling and seismic anisotropy. The modeling suggests that the northernmost part of the ridge consists of Icelandic type oceanic crust, bordered to the north by anomalously thick oceanic crust formed at the Mohns spreading ridge. The modeled Vp/Vs-ratios suggest variations in gabbroic composition and present-day temperatures in the area. Anisotropy analysis reveals a fast S-wave component along the Jan Mayen Ridge. This pattern of anisotropy is most readily interpreted as dikes intruded along the ridge, suggesting that the magmatism can be related to the development of a leaky transform since Early Oligocene

Crustal structure of the ultra-slow spreading Knipovich Ridge, North Atlantic, along a presumed ridge segment center

A combined ocean bottom seismometer, multichannel seismic reflection and gravity study has been carried out along the spreading direction of the Knipovich Ridge over a topographic high that defines a segment center. The youngest parts of the crust in the immediate vicinity of the ridge reveal fractured Oceanic Layer 2 and thermally expanded and possibly serpentinized Oceanic Layer 3. The mature part of the crust has normal thickness and seismic velocities with no significant crustal thickness and seismic velocity variations. Mature Oceanic Layer 2 is in addition broken into several rotated fault blocks. Comparison with a profile acquired *40 km north of the segment center reveals significant differences. Along this profile, reported earlier, periods of slower spreading led to generation of thin crust with a high P-wave velocity (Vp), composed of a mixture of gabbro and serpentinized mantle, while periods of faster spreading led to generation of more normal gabbroic crust. For the profile across the segment center no clear relation exists between spreading rate and crustal thickness and seismic velocity. In this study we have found that higher magmatism may lead to generation of oceanic crust with normal thickness even at ultra-slow spreading rates