Telomere structure and shortening in telomerase-deficient Trypanosoma brucei

Telomerase consists of a reverse transcriptase (TERT) and an RNA that contains a template for telomere-repeat extension. Telomerase is required to prevent telomere erosion and its activity or lack thereof is important for tumorigenesis and ageing. Telomerase has been identified in numerous organisms but it has not been studied in kinetoplastid protozoa. Trypanosoma brucei, the causative agent of African sleeping sickness, evades the host immune response by frequently changing its variant surface glycoprotein (VSG). The single expressed VSG is transcribed from one of ∼20 subtelomeric ‘Expression Sites’, but the role telomeres might play in regulating VSG transcription and switching is unknown. We identified and sequenced the T.brucei TERT gene. Deleting TERT resulted in progressive telomere shortening of 3–6 bp per generation. In other organisms, the rate of telomere shortening is proportional to the length of the terminal 3′ single-strand overhang. In T.brucei, G-overhangs were undetectable (<30 nt) by in-gel hybridization. The rate of telomere shortening therefore, agrees with the predicted shortening due to the end replication problem, and is consistent with our observation that G-overhangs are short. Trypanosomes whose telomere length can be manipulated provide a new tool to investigate the role of telomeres in antigenic variation

Blue Variable Stars from the MACHO database I: Photometry and Spectroscopy of the LMC sample

We present the photometric properties of 1279 blue variable stars within the LMC. Photometry is derived from the MACHO database. The lightcurves of the sample exhibit a variety of quasi-periodic and aperiodic outburst behavior. A characteristic feature of the photometric variation is that the objects are reddest when at maximum outburst. A subset of 102 objects were examined spectroscopically. Within this subset, 91% exhibited Balmer emission in at least one epoch, in some cases with spectacular spectral variability. The variability observed in the sample is consistent with the establishment and maintenance of the Be phenomenon.Comment: 19 pages, AJ accepte

Explaining UXOR variability with self-shadowed disks

In this Letter we propose a new view on UX Orionis type variability. The idea is based on the earlier proposal by various authors that UXORs are nearly-edge-on disks in which hydrodynamic fluctuations could cause clumps of dust and gas to cross the line of sight. However, because the standard disk models have a flaring geometry, it is mostly the outer regions of the disk that obscure the star. The time scales for such obscuration events would be too long to match the observed time scales of weeks to months. Recent 2-D self-consistent models of Herbig Ae/Be protoplanetary disks (Dullemond et al. 2002,2003 henceforth D02/DD03), however, have indicated that for Herbig Ae/Be star disks there exists, in addition to the usual flared disks, also a new class of disks: those that are fully self-shadowed. Only their puffed-up inner rim (at the dust evaporation radius) is directly irradiated by the star, while the disk at larger radius resides in the shadow of the rim. For these disks there exist inclinations at which the line of sight towards the star skims the upper parts of the puffed-up inner rim, while passing high over the surface of outer disk regions. Small hydrodynamic fluctuations in the puffed-up inner rim could then be held responsible for the extinction events seen in UXORs. If this idea is correct, it makes a prediction for the shape of the SEDs of these stars. It was shown by D02/DD03 that flared disks have a strong far-IR excess and can be classified as `group I' (in the classification of Meeus et al. 2001), while self-shadowed disks have a relatively weak far-IR excess and are classified as `group II'. Our model therefore predicts that UXORs belong to the `group II' sources. We show that this correlation is indeed found within a sample of 86 Herbig Ae/Be stars.Comment: Accepted for publication in ApJ Letters (a few lines added to original version to accommodate comments of referee

Chiral and herringbone symmetry breaking in water-surface monolayers

We report the observation from monolayers of eicosanoic acid in the L′2 phase of three distinct out-of-plane first-order diffraction peaks, indicating molecular tilt in a nonsymmetry direction and hence the absence of mirror symmetry. At lower pressures the molecules tilt in the direction of their nearest neighbors. In this region we find a structural transition, which we tentatively identify as the rotator-herringbone transition L2d−L2h

Phase Behaviour of Amphiphilic Monolayers: Theory and Simulation

Coarse grained models of monolayers of amphiphiles (Langmuir monolayers) have been studied theoretically and by computer simulations. We discuss some of the insights obtained with this approach, and present new simulation results which show that idealised models can successfully reproduce essential aspects of the generic phase behaviour of Langmuir monolayers.Comment: To appear in J. Phys.: Cond. Matte

Relation between the luminosity of young stellar objects and their circumstellar environment

We present a new model-independent method of comparison of NIR visibility data of YSOs. The method is based on scaling the measured baseline with the YSO's distance and luminosity, which removes the dependence of visibility on these two variables. We use this method to compare all available NIR visibility data and demonstrate that it distinguishes YSOs of luminosity >1000L_sun (low-L) from YSOs of <1000L_sun (high-L). This confirms earlier suggestions, based on fits of image models to the visibility data, for the difference between the NIR sizes of these two luminosity groups. When plotted against the ``scaled'' baseline, the visibility creates the following data clusters: low-L Herbig Ae/Be stars, T Tauri stars, and high-L Herbig Be stars. The T Tau cluster is similar to the low-L Herbig Ae/Be cluster, which has ~7 times smaller ``scaled'' baselines than the high-L Herbig Be cluster. We model the shape and size of clusters with different image models and find that low-L Herbig stars are the best explained by the uniform brightness ring and the halo model, T Tauri stars with the halo model, and high-L Herbig stars with the accretion disk model. However, the plausibility of each model is not well established. Therefore, we try to build a descriptive model of the circumstellar environment consistent with various observed properties of YSOs. We argue that low-L YSOs have optically thick disks with the optically thin inner dust sublimation cavity and an optically thin dusty outflow above the inner disk regions. High-L YSOs have optically thick accretion disks with high accretion rates enabling gas to dominate the NIR emission over dust. Although observations would favor such a description of YSOs, the required dust distribution is not supported by our current understanding of dust dynamics.Comment: 20 pages, 12 figures, Accepted for publication in the Astrophysical Journa

VLT/NACO adaptive optics imaging of the TY CrA system - A fourth stellar component candidate detected

We report the detection of a possible subsolar mass companion to the triple young system TY CrA using the NACO instrument at the VLT UT4 during its commissioning. Assuming for TY CrA a distance similar to that of the close binary system HD 176386, the photometric spectral type of this fourth stellar component candidate is consistent with an ~M4 star. We discuss the dynamical stability of this possible quadruple system as well as the possible location of dusty particles inside or outside the system.Comment: 4 pages, 2 figures postscrip

Inner disc rearrangement revealed by dramatic brightness variations in the young star PV Cep

Young Sun-like stars at the beginning of the pre-main sequence (PMS) evolution are surrounded by accretion discs and remnant protostellar envelopes. Photometric and spectroscopic variations of these stars are driven by interactions of the star with the disc. Time scales and wavelength dependence of the variability carry information on the physical mechanisms behind these interactions. We conducted multi-epoch, multi-wavelength study of PV Cep, a strongly variable, accreting PMS star. By combining our own observations from 2004-2010 with archival and literature data, we show that PV Cep started a spectacular fading in 2005, reaching an I_C-band amplitude of 4 mag. Analysis of variation of the optical and infrared fluxes, colour indices, and emission line fluxes suggests that the photometric decline in 2005-2009 resulted from an interplay between variable accretion and circumstellar extinction: since the central luminosity of the system is dominated by accretion, a modest drop in the accretion rate could induce the drastic restructuring of the inner disc. Dust condensation in the inner disc region might have resulted in the enhancement of the circumstellar extinction.Comment: 11 pages, 4 figures, accepted for publication by MNRAS. 3 online tables adde

Magnetospheres and Disk Accretion in Herbig Ae/Be Stars

We present evidence of magnetically-mediated disk accretion in Herbig Ae/Be stars. Magnetospheric accretion models of Balmer and sodium profiles calculated with appropriate stellar and rotational parameters are in qualitative agreement with the observed profiles of the Herbig Ae star UX Ori, and yield a mass accretion rate of ~ 10^{-8} Msun/yr. If more recent indications of an extremely large rotation rate for this object are correct, the magnetic field geometry must deviate from that of a standard dipole in order to produce line emission consistent with observed flux levels. Models of the associated accretion shock qualitatively explain the observed distribution of excess fluxes in the Balmer discontinuity for a large ensemble of Herbig Ae/Be stars, and imply typically small mass accretion rates, < 10^{-7} Msun/yr. In order for accretion to proceed onto the star, significant amounts of gas must exist inside the dust destruction radius, which is potentially problematic for recently advocated scenarios of "puffed" inner dust wall geometries. However, our models of the inner gas disk show that for the typical accretion rates we have derived, the gas should be generally optically thin, thus allowing direct stellar irradiation of the inner dust edge of the disk.Comment: 32 pages, 12 figures, accepted by Ap

Herringbone ordering and lattice distortions in a planar-molecule model for Langmuir monolayers

A model of planar molecules, made up of "atoms" interacting by Lennard-Jones potentials and arranged to mimic the cross section of alkyl chains, is used to study the problem of backbone plane ordering in Langmuir monolayers. It is shown that two minima of the interaction energy are reached if molecules lie on the sites of a centered rectangular lattice in a herringbone configuration with two different dihedral angles. These orientationally ordered phases can be related to the so-called herringbone and pseudoherringbone structures, whose lattice distortions qualitatively agree with those determined by means of grazing incidence x-ray diffraction experiments on Langmuir monolayers. A third energy minimum is obtained for a configuration of parallel molecules on an oblique lattice, which has also been observed in some experiments. The competition between the three phases is investigated, upon varying geometric parameters of the model molecules and surface pressure. The effect of temperature is analyzed in a mean field approximation, by taking into account the orientational entropy contribution on a lattice system with variable unit cell parameters. In this framework the transition to an orientationally disordered phase is also pointed out