A COMPARATIVE CLINICAL STUDY ON THE EFFECTIVENESS OF BHARANGYADI CHOORNA AND VYAGHRI CHOORNA IN TAMAKA SWASA (BRONCHIAL ASTHMA)

Ayurveda classics mentioned various types of Swasa and Tamaka Swasa is one among them. Tamakaswasa is manifested by aggravated Pranavayu by the obstruction of Kapha. In this case treatment should be to clear out Pranavaha srotas, pacify Vata and remove the blockage due to Kapha. In modern science Tamaka Swasa is correlated with Bronchial Asthma. It’s a chronic inflammatory disorder of the airways in which the chronic inflammation causes an associated increase in airway hyper responsiveness that leads to recurrent episodes of asthmatic exacerbation. Modern science has no permanent cure of Tamaka Swasa, that’s why it is necessity to search herbal and herbo-mineral preparations for the treatment of disease. Present Study was conducted to reduce the symptoms of Tamakaswasa. Bharangyadi Choorna and Vyaghri Choorna have the properties of Kapha Vata hara, Agni Deepana, Pachana, Anulomana, Srotoshodhana, anti-asthamatic and anti-inflammatory property. Materials and Method: Patients who have symptoms of Tamakaswasa fulfill the inclusion criteria were given with Bharangyadi Choorna 4gm thrice a day along with Ardraka Swarasa as Anupana in the trail group i.e., group A and Vyaghri Choorna 4gm thrice a day along with honey as Anupana in the control group i.e. group B. It is a comparative clinical study with 30 patients in each group for 30 days. Analyzing the signs and symptoms, PEFR after each 10 days, Wilcoxon test was done for comparing the effectiveness of treatment between two groups. Comparative analysis of the overall effect of the treatments in both the groups was done by statistically done by Mann-Whitney test. Results: There was statistically significant change in all the signs and symptoms and PEFR after treatment and follow up. All the signs and symptoms have P ≤ 0.05. Conclusion: Bharangyadi Choorna has shown highly significant reduction in the symptoms like Swasakrichratha, Peenasa, Kasa, Ghurghuraka, Krichrabhashana, Shushkasya and PEFR. On comparison between the two groups, Bharangyadi choorna showed a better result in improvement of symptoms- Swasakrichratha, Peenasa, Kasa, Ghurghuraka, Krichrabhashana, Shushkasya and objective parameter- PEFR. Hence H2 hold good

A comparitive pharmaceutico analytical study of Nishamalaki Vati

Vati Kalpana is the widely used dosage forms because of its advantages like palatability, easy transportation and fixation of dose; an effort is made to analyze the organoleptic, physical and analytical changes with and without addition of starch in the preparation of Nishamalaki Vati. Here, a small attempt of two different pharmaceutico-analytical techniques is implied in making of Nishamalaki Vati which may bring change in pharmaceutical science of Ayurveda

S-nitrosation of proteins relevant to Alzheimer's disease during early stages of neurodegeneration

Protein S-nitrosation (SNO-protein), the nitric oxide-mediated posttranslational modification of cysteine thiols, is an important regulatory mechanism of protein function in both physiological and pathological pathways. A key first step toward elucidating the mechanism by which S-nitrosation modulates a protein's function is identification of the targeted cysteine residues. Here, we present a strategy for the simultaneous identification of SNO-cysteine sites and their cognate proteins to profile the brain of the CK-p25-inducible mouse model of Alzheimer's disease-like neurodegeneration. The approach-SNOTRAP (SNO trapping by triaryl phosphine)-is a direct tagging strategy that uses phosphinebased chemical probes, allowing enrichment of SNO-peptides and their identification by liquid chromatography tandem mass spectrometry. SNOTRAP identified 313 endogenous SNO-sites in 251 proteins in the mouse brain, of which 135 SNO-proteins were detected only during neurodegeneration. S-nitrosation in the brain shows regional differences and becomes elevated during early stages of neurodegeneration in the CK-p25 mouse. The SNO-proteome during early neurodegeneration identified increased S-nitrosation of proteins important for synapse function, metabolism, and Alzheimer's disease pathology. In the latter case, proteins related to amyloid precursor protein processing and secretion are S-nitrosated, correlating with increased amyloid formation. Sequence analysis of SNO-cysteine sites identified potential linear motifs that are altered under pathological conditions. Collectively, SNOTRAP is a direct tagging tool for global elucidation of the SNO-proteome, providing functional insights of endogenous SNO proteins in the brain and its dysregulation during neurodegeneration.National Institutes of Health (U.S.) (Grant CA26731)National Institutes of Health (U.S.) (Grant R01 NS051874

S-nitrosation of proteins relevant to Alzheimer’s disease during early stages of neurodegeneration

Protein S-nitrosation (SNO-protein), the nitric oxide-mediated posttranslational modification of cysteine thiols, is an important regulatory mechanism of protein function in both physiological and pathological pathways. A key first step toward elucidating the mechanism by which S-nitrosation modulates a protein’s function is identification of the targeted cysteine residues. Here, we present a strategy for the simultaneous identification of SNO-cysteine sites and their cognate proteins to profile the brain of the CK-p25–inducible mouse model of Alzheimer’s disease-like neurodegeneration. The approach—SNOTRAP (SNO trapping by triaryl phosphine)—is a direct tagging strategy that uses phosphine-based chemical probes, allowing enrichment of SNO-peptides and their identification by liquid chromatography tandem mass spectrometry. SNOTRAP identified 313 endogenous SNO-sites in 251 proteins in the mouse brain, of which 135 SNO-proteins were detected only during neurodegeneration. S-nitrosation in the brain shows regional differences and becomes elevated during early stages of neurodegeneration in the CK-p25 mouse. The SNO-proteome during early neurodegeneration identified increased S-nitrosation of proteins important for synapse function, metabolism, and Alzheimer’s disease pathology. In the latter case, proteins related to amyloid precursor protein processing and secretion are S-nitrosated, correlating with increased amyloid formation. Sequence analysis of SNO-cysteine sites identified potential linear motifs that are altered under pathological conditions. Collectively, SNOTRAP is a direct tagging tool for global elucidation of the SNO-proteome, providing functional insights of endogenous SNO proteins in the brain and its dysregulation during neurodegeneration.National Institutes of Health (U.S.) (NIH Grant CA26731)Massachusetts Institute of Technology. Center for Environmental Health Sciences (Grant ES002109)Simons FoundationNational Institutes of Health (U.S.) (NIH Grant R01 NS051874

Cholesterol Granuloma of Maxillary Sinus – An Unusual Case

Introduction Cholesterol granuloma is a type of foreign body granuloma found in tissues wherein the cholesterol crystals get accumulated. Quite unusual to be present in maxillary sinus owing to its pathogenesis. Case Report A 12 year old male child diagnosed with antrochoanal polyp, underwent endoscopic sinus surgery and the microscopic analyses revealed maxillary sinus cholesterol granuloma. Discussion Cholesterol granuloma is an uncommon tissue reaction to cholesterol crystals in the maxillary sinus owing to its well-ventilated state and is frequently associated with chronic sinuses disease or trauma. Since its signs and symptoms are non–specific, histopathological analysis is essential for correct diagnosis

Bacterial Pneumonia and Pandemic Influenza Planning

Prevention and treatment of secondary bacterial complications are important but neglected areas of planning

Changes in resting-state functional brain activity are associated with waning cognitive functions in HIV-infected children.

Delayed brain development in perinatally HIV-infected children may affect the functional brain activity and subsequently cognitive function. The current study evaluated the functional brain activity in HIV-infected children by quantifying the amplitude of low frequency fluctuations (ALFF) and functional connectivity (FC). Additionally, correlation of ALFF and FC with cognitive measures was performed. Twenty-six HIV-infected children and 20 control children underwent neuropsychological (NP) assessment and resting-state functional magnetic resonance imaging (rs-fMRI). ALFF and FC maps were generated and group differences were analyzed using two-sample t-test. Furthermore, ALFF and FC showing significant group differences were correlated with NP scores using Pearson's correlation. Significantly lower ALFF in the left middle temporal gyrus, precentral and post central gyrus was observed in HIV-infected children compared to controls. FC was significantly reduced in the right inferior parietal, vermis, middle temporal and left postcentral regions, and significantly increased in the right precuneus, superior parietal and left middle frontal regions in HIV-infected children as compared to control. HIV-infected children showed significantly lower NP scores in various domains including closure, exclusion, memory, verbal meaning, quantity and hidden figure than controls. These waning cognitive functions were significantly associated with changes in ALFF and FC in HIV-infected children. The findings suggest that abnormal ALFF and FC may responsible for cognitive deficits in HIV-infected children. ALFF and FC in association with cognitive evaluation may provide a clinical biomarker to evaluate functional brain activity and to plan neurocognitive intervention in HIV-infected children undergoing standard treatment.This study was funded by Department of Science and Technology, New Delhi, India (Grant number: SR/CSI/02/2 0 10, G) and Sidra Medicine, Doha, Qatar, has provided the workstation for image processing

Large-area growth of MoS2 at temperatures compatible with integrating back-end-of-line functionality

Direct growth of transition metal dichalcogenides over large areas within the back-end-of-line (BEOL) thermal budget limit of silicon integrated circuits is a significant challenge for 3D heterogeneous integration. In this work, we report on the growth of MoS2 films (~1-10 nm) on SiO2, amorphous-Al2O3, c-plane sapphire, and glass substrates achieved at low temperatures (350 C-550 C) by chemical vapor deposition in a manufacturing-compatible 300 mm atomic layer deposition reactor. We investigate the MoS2 films as a potential material solution for BEOL logic, memory and sensing applications. Hall-effect/4-point measurements indicate that the ~10 nm MoS2 films exhibit very low carrier concentrations (1014-1015 cm-3), high resistivity, and Hall mobility values of ~0.5-17 cm2 V-1 s-1, confirmed by transistor and resistor test device results. MoS2 grain boundaries and stoichiometric defects resulting from the low thermal budget growth, while detrimental to lateral transport, can be leveraged for the integration of memory and sensing functions. Vertical transport memristor structures (Au/MoS2/Au) incorporating ~3 nm thick MoS2 films grown at 550 C (~0.75 h) show memristive switching and a stable memory window of 105 with a retention time >104 s, between the high-low resistive states. The switching set and reset voltages in these memristors demonstrate a significant reduction compared to memristors fabricated from pristine, single-crystalline MoS2 at higher temperatures, thereby reducing the energy needed for operation. Furthermore, interdigitated electrode-based gas sensors fabricated on ~5 nm thick 550 C-grown (~1.25 h) MoS2 films show excellent selectivity and sub-ppm sensitivity to NO2 gas, with a notable self-recovery at room temperature. The demonstration of large-area MoS2 direct growth at and below the BEOL thermal budget limit, alongside memristive and gas sensing functionality, advances a key enabling technology objective in emerging materials and devices for 3D heterogeneous integration