Response and recovery of syntrophic and methanogenic activity to saltwater intrusion in a tidal freshwater marsh soil

Tidal freshwater wetland soils contain large amounts of organic carbon, some of which is mineralized to carbon dioxide (CO2) and methane (CH4) by a diverse consortium of anaerobic microorganisms that includes fermenters, syntrophs, and methanogens (MG). These microbial groups are tightly linked and often rely on cooperative interspecies metabolisms (i.e., syntrophy) to survive. Environmental perturbations can disrupt these interactions and thus alter the rates and pathways of carbon cycling. One environmental change of particular concern in coastal wetlands is sea level rise, which can result in increased episodic saltwater intrusion events into these ecosystems. These events cause an influx of sulfate (SO4-2) to the soils and may stimulate sulfate-reducing bacteria (SRB), which can directly compete with syntrophs for energy sources (e.g., fermentation products such as butyrate). Since syntroph metabolism generates byproducts that serve as the energy source for many MG, this competition can have indirect negative effects on methanogenesis. In addition, SRB can directly compete with MG for these byproducts, particularly formate, H2, and/or acetate. The goal of this study was to understand how both MG and syntroph-MG consortia respond to and recover from SRB competition during an episodic saltwater intrusion event. To achieve this, microcosms containing soil slurry from a freshwater wetland were subjected to simulated saltwater intrusion, and metabolic inhibitors were used to isolate the activity of the various functional groups. This study focused on the breakdown of butyrate, which is a key energy source in syntroph‑MG consortia metabolisms. The observed changes in butyrate breakdown rates and byproduct accumulation during butyrate degradation assays confirmed that butyrate breakdown was mediated through syntroph-MG consortia, and that formate, rather than H2, was likely used as an electron carrier during syntrophic activity. Additions of SO4‑2 (as Na2SO4) to the freshwater microcosms stimulated SRB activity and shifted the MG community to favor acetoclastic members. These changes were accompanied by a 24% increase in CO2 production and an 80% decrease in CH4 production. Interestingly, when NaCl was added to achieve similar ionic strength, CH4 production decreased by ~32%, suggesting SRB competition is not the only factor affecting methanogenesis. Butyrate degradation rates demonstrated that while SRB were strong competitors for butyrate, concurrent syntrophic metabolism was possible. Further, data show that SRB were poor competitors for acetate, which could explain the increase in acetoclastic MG. Following removal of SRB competition, CH4 production recovered but only by ~50% after 28 days, which suggests that some MG communities in tidal freshwater wetlands may not be resilient to saltwater intrusion events. Over this same time, rates of syntrophic butyrate breakdown largely recovered, but butyrate breakdown resulted in the production of less CH4 and acetate and more CO2 and formate, indicating saltwater intrusion events may lead to persistent changes in the byproducts and pathways of carbon breakdown in tidal freshwater wetlands

Assessing how disruption of methanogenic communities and their syntrophic relationships in tidal freshwater marshes via saltwater intrusion may affect CH4 emissions

Tidal freshwater wetlands (TFW), which lie at the interface of saltwater and freshwater ecosystems, are predicted to experience moderate salinity increases due to sea level rise. Increases in salinity generally suppress CH4 production, but it is uncertain to what extent elevated salinity will affect CH4 cycling in TFW. It is also unknown whether CH4 production will resume when freshwater conditions return. The ability to produce CH4 is limited to a monophyletic group of the Euryarchaeota phylum called methanogens (MG), who are limited to a small number of substrates (e.g., acetate, H2, and formate) produced from the breakdown of fermentation products. In freshwater anaerobic soils, the degradation of certain fermentation products (e.g., butyrate, propionate) is only energetically favorable when their catabolic byproduct, H2 or formate, is consumed to low concentrations by MGs. This is considered a form of obligate syntrophy. Sulfate reducing bacteria (SRB) are capable of utilizing a larger variety of substrates than MG, including substrates degraded by methanogenic syntrophy (e.g., butyrate, propionate). The introduction of sulfate (SO4 -2) into TFW via saltwater intrusion events may allow SRB to disrupt syntrophic relationships between hydrogenotrophic MG and syntrophic fermenters. This may select for MG taxa that differ in their rate of CH4 production. The objectives of this study were to determine the effect of oligohaline SO4 -2 concentrations on MG community functions (i.e., CH4 production and syntrophic butyrate degradation); and, to assess whether these functions recover after competition with SRB has been removed

Force-induced remodelling of proteins and their complexes

Force can drive conformational changes in proteins, as well as modulate their stability and the affinity of their complexes, allowing a mechanical input to be converted into a biochemical output. These properties have been utilised by nature and force is now recognised to be widely used at the cellular level. The effects of force on the biophysical properties of biological systems can be large and varied. As these effects are only apparent in the presence of force, studies on the same proteins using traditional ensemble biophysical methods can yield apparently conflicting results. Where appropriate, therefore, force measurements should be integrated with other experimental approaches to understand the physiological context of the system under study

Integrin Clustering Is Driven by Mechanical Resistance from the Glycocalyx and the Substrate

Integrins have emerged as key sensory molecules that translate chemical and physical cues from the extracellular matrix (ECM) into biochemical signals that regulate cell behavior. Integrins function by clustering into adhesion plaques, but the molecular mechanisms that drive integrin clustering in response to interaction with the ECM remain unclear. To explore how deformations in the cell-ECM interface influence integrin clustering, we developed a spatial-temporal simulation that integrates the micro-mechanics of the cell, glycocalyx, and ECM with a simple chemical model of integrin activation and ligand interaction. Due to mechanical coupling, we find that integrin-ligand interactions are highly cooperative, and this cooperativity is sufficient to drive integrin clustering even in the absence of cytoskeletal crosslinking or homotypic integrin-integrin interactions. The glycocalyx largely mediates this cooperativity and hence may be a key regulator of integrin function. Remarkably, integrin clustering in the model is naturally responsive to the chemical and physical properties of the ECM, including ligand density, matrix rigidity, and the chemical affinity of ligand for receptor. Consistent with experimental observations, we find that integrin clustering is robust on rigid substrates with high ligand density, but is impaired on substrates that are highly compliant or have low ligand density. We thus demonstrate how integrins themselves could function as sensory molecules that begin sensing matrix properties even before large multi-molecular adhesion complexes are assembled

Dynamics of Citrate Coordination on Gold Nanoparticles Under Low Specific Power Laser‐Induced Heating

International audienceSERS evolution recorded over a drop‐coated coffee‐ring pattern of citrate‐capped gold colloids was investigated as a function of time under low‐specific laser power. Spectral changes caused by plasmon‐induced reaction could not be detected, but a long‐term transient original spectral profile showing additional lines was observed. We performed deep qualitative and quantitative SERS intensity variation analysis based on the complementary use of extreme deviation and cross‐correlation statistics, which provided further insights on the behavior of citrate‐capping layers of gold nanoparticles upon laser illumination. More precisely, the cross‐correlation analysis made possible to follow the so‐called individual events denoting particular resonance structures, in which groups of modes were assigned to an evolution of citrate coordination on gold surface driven by photo‐activation. As a consequence, the detection limit was increased and new lines were related to the presence of a very low amount of dicarboxy‐acetone (DCA), which was already present in the system

The structure of the merging RCS 231953+00 supercluster at z ~ 0.9

The RCS2319+00 supercluster is a massive supercluster at z = 0.9 comprising three optically selected, spectroscopically confirmed clusters separated by <3Mpc on the plane of the sky. This supercluster is one of a few known examples of the progenitors of present-day massive clusters (1015 M ☉ by z ~ 0.5). We present an extensive spectroscopic campaign carried out on the supercluster field resulting, in conjunction with previously published data, in 1961 high-confidence galaxy redshifts. We find 302 structure members spanning three distinct redshift walls separated from one another by ~65Mpc (Δ z = 0.03). The component clusters have spectroscopic redshifts of 0.901, 0.905, and 0.905. The velocity dispersions are consistent with those predicted from X-ray data, giving estimated cluster masses of ~10 14.5-10 14.9 M ☉. The Dressler-Shectman test finds evidence of substructure in the supercluster field and a friends-of-friends analysis identified five groups in the supercluster, including a filamentary structure stretching between two cluster cores previously identified in the infrared by Coppin etal. The galaxy colors further show this filamentary structure to be a unique region of activity within the supercluster, comprised mainly of blue galaxies compared to the ~43%-77% red-sequence galaxies present in the other groups and cluster cores. Richness estimates from stacked luminosity function fits result in average group mass estimates consistent with ~1013 M ☉ halos. Currently, 22% of our confirmed members reside in 1013 M ☉ groups/clusters destined to merge onto the most massive cluster, in agreement with the massive halo galaxy fractions important in cluster galaxy pre-processing in N-body simulation merger tree studiesPeer reviewe